ES2114504A1 - Method for the enzyme synthesis of deoxyoligonucleotides on a solid substrate - Google Patents
Method for the enzyme synthesis of deoxyoligonucleotides on a solid substrateInfo
- Publication number
- ES2114504A1 ES2114504A1 ES09601945A ES9601945A ES2114504A1 ES 2114504 A1 ES2114504 A1 ES 2114504A1 ES 09601945 A ES09601945 A ES 09601945A ES 9601945 A ES9601945 A ES 9601945A ES 2114504 A1 ES2114504 A1 ES 2114504A1
- Authority
- ES
- Spain
- Prior art keywords
- solid substrate
- deoxyoligonucleotides
- enzyme synthesis
- synthesis
- terminal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Method for the enzyme synthesis of deoxyoligonucleotides on a solid substrate, which comprises performing the synthesis on a solid substrate in the (5') to (3') direction using terminal transferase, a DNA polymerase regardless of type which preferentially catalyses the incorporation of deoxyribonucleoside (5') triphosphates to single chain DNA terminal (3') hydroxyl and uses deoxyribonucleotides with their (3') hydroxyls temporarily protected in order to add a single nucleotide in each cycle.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9601945A ES2114504B1 (en) | 1996-09-13 | 1996-09-13 | METHOD FOR THE ENZYMATIC SYNTHESIS OF DEOXYOLIGONUCLEOTIDES IN SOLID SUPPORT. |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES9601945A ES2114504B1 (en) | 1996-09-13 | 1996-09-13 | METHOD FOR THE ENZYMATIC SYNTHESIS OF DEOXYOLIGONUCLEOTIDES IN SOLID SUPPORT. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2114504A1 true ES2114504A1 (en) | 1998-05-16 |
ES2114504B1 ES2114504B1 (en) | 1999-02-01 |
Family
ID=8296028
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES9601945A Expired - Fee Related ES2114504B1 (en) | 1996-09-13 | 1996-09-13 | METHOD FOR THE ENZYMATIC SYNTHESIS OF DEOXYOLIGONUCLEOTIDES IN SOLID SUPPORT. |
Country Status (1)
Country | Link |
---|---|
ES (1) | ES2114504B1 (en) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4605735A (en) * | 1983-02-14 | 1986-08-12 | Wakunaga Seiyaku Kabushiki Kaisha | Oligonucleotide derivatives |
US4689405A (en) * | 1983-01-20 | 1987-08-25 | Gesellschaft Fur Biotechnologische Forschung Mbh (Gbf) | Process for the simultaneous synthesis of several oligonucleotides on a solid phase |
US4816571A (en) * | 1987-06-04 | 1989-03-28 | Applied Biosystems, Inc. | Chemical capping by phosphitylation during oligonucleotide synthesis |
WO1992009615A1 (en) * | 1990-11-26 | 1992-06-11 | Pharmacia Lkb Biotechnology Ab | Method and means for oligonucleotide synthesis |
WO1996007669A1 (en) * | 1994-09-02 | 1996-03-14 | Rose-Hiatt Biotechnology Partnership | Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides |
-
1996
- 1996-09-13 ES ES9601945A patent/ES2114504B1/en not_active Expired - Fee Related
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4689405A (en) * | 1983-01-20 | 1987-08-25 | Gesellschaft Fur Biotechnologische Forschung Mbh (Gbf) | Process for the simultaneous synthesis of several oligonucleotides on a solid phase |
US4605735A (en) * | 1983-02-14 | 1986-08-12 | Wakunaga Seiyaku Kabushiki Kaisha | Oligonucleotide derivatives |
US4816571A (en) * | 1987-06-04 | 1989-03-28 | Applied Biosystems, Inc. | Chemical capping by phosphitylation during oligonucleotide synthesis |
WO1992009615A1 (en) * | 1990-11-26 | 1992-06-11 | Pharmacia Lkb Biotechnology Ab | Method and means for oligonucleotide synthesis |
WO1996007669A1 (en) * | 1994-09-02 | 1996-03-14 | Rose-Hiatt Biotechnology Partnership | Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides |
Also Published As
Publication number | Publication date |
---|---|
ES2114504B1 (en) | 1999-02-01 |
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Legal Events
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EC2A | Search report published |
Date of ref document: 19980516 Kind code of ref document: A1 Effective date: 19980516 |
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Effective date: 20161227 |