ES2112302T3 - Metodo para producir un polinucleotido para uso en amplificacion con un unico cebador y oligonucleotidos que contienen fosforotioato como cebadores para la amplificacion de acidos nucleicos. - Google Patents
Metodo para producir un polinucleotido para uso en amplificacion con un unico cebador y oligonucleotidos que contienen fosforotioato como cebadores para la amplificacion de acidos nucleicos.Info
- Publication number
- ES2112302T3 ES2112302T3 ES92309231T ES92309231T ES2112302T3 ES 2112302 T3 ES2112302 T3 ES 2112302T3 ES 92309231 T ES92309231 T ES 92309231T ES 92309231 T ES92309231 T ES 92309231T ES 2112302 T3 ES2112302 T3 ES 2112302T3
- Authority
- ES
- Spain
- Prior art keywords
- polynucleotide
- extender probe
- amplification
- sequence
- target sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
SE PRESENTA UN METODO PARA EXTENDER UNA SONDA EXTENDEDORA PARA PRODUCIR UN POLIDEOXINUCLEOTIDO RAMIFICADO SIMPLE QUE ESTA LIBRE DE SONDAS EXTENDEDORAS NO REACCIONADAS Y QUE TIENE DOS SEGMENTOS QUE NO SON CONTIGUOS Y QUE SON COMPLEMENTARIOS UNO CON RESPECTO AL OTRO. EL METODO COMPRENDE LOS PASOS DE (1) SUMINISTRAR EN COMBINACION (A) UN POLINUCLEOTIDO QUE TENGA DOS SECUENCIAS NUCLEOTIDAS CONTIGUAS NO COMPLEMENTARIAS S1 Y S2 EN DONDE S2 ES 5'' DE S1 Y AL MENOS DIEZ DEOXINUCLEOTIDOS DE LONGITUD, (B) UNA SONDA EXTENDEDORA COMPUESTA DE DOS SECUENCIAS DEOXINUCLEOTIDAS, EN DONDE LA SECUENCIA EN EL EXTREMO 3'' DE LA SONDA EXTENDEDORA (EP1) ES HIBRIDIZABLE CON S1 Y LA OTRA SECUENCIA DE OXINUCLEOTIDA (EP2) ES SUBSTANCIALMENTE IDENTICA A S2 Y (C) MEDIOS PARA MODIFICAR EL EXTREMO 3'' DE LA SONDA EXTENDEDORA QUE NO SE HIBRIDIZA CON EL POLINUCLEOTIDO Y (2) EXTENDIENDO LA SONDA EXTENDEDORA A LO LARGO DEL POLINUCLEOTIDO EN DONDE LA SONDA EXTENDEDORA NO HIBRIDIZADA CON EL POLINUCLEOTIDO SE MODIFICA EN SU EXTREMO 3''. TAMBIEN SE PRESENTA UN METODO PARA FORMAR UNA SECUENCIA POLINUCLEOTIDA COMPLEMENTARIA DE UNA SECUENCIA POLINUCLEOTIDA RAMIFICADA DE INTERES. EN EL METODO UN POLINUCLEOTIDO PRIMARIO QUE TENGA UN TERMINAL 3'' COMPUESTO DE UN MONOFOSFATO NUCLEOTIDO, QUE TENGA AL MENOS UN ENLACE FOSFATO-AZUFRE, SE HIBRIDIZA CON EL EXTREMO 3'' DE LA SECUENCIA DE INTERES. EL POLINUCLEOTIDO INICIAL SE EXTIENDE A LO LARGO DE LA SECUENCIA DE INTERES Y EL POLINUCLEOTIDO INICIAL EXTENDIDO SE DISOCIA ENTONCES DE LA SECUENCIA DE INTERES. EL METODO ENCUENTRA UNA APLICACION PARTICULAR EN EL AREA DE LA AMPLIFICACION DEL ACIDO NUCLEICO.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US77653891A | 1991-10-11 | 1991-10-11 | |
US92307092A | 1992-07-31 | 1992-07-31 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2112302T3 true ES2112302T3 (es) | 1998-04-01 |
Family
ID=27119199
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES92309231T Expired - Lifetime ES2112302T3 (es) | 1991-10-11 | 1992-10-09 | Metodo para producir un polinucleotido para uso en amplificacion con un unico cebador y oligonucleotidos que contienen fosforotioato como cebadores para la amplificacion de acidos nucleicos. |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP0549107B1 (es) |
JP (1) | JPH07143900A (es) |
AT (1) | ATE161586T1 (es) |
CA (1) | CA2080305A1 (es) |
DE (1) | DE69223755T2 (es) |
DK (1) | DK0549107T3 (es) |
ES (1) | ES2112302T3 (es) |
Families Citing this family (32)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5525462A (en) * | 1991-05-02 | 1996-06-11 | Toyo Boseki Kabushiki Kaisha | Nucleic acid sequence amplification method, detection method, and reagent kit therefor |
ZA929319B (en) * | 1991-12-11 | 1993-05-24 | Igen Inc | Method for exponential amplification of nucleic acid by a single unpaired primer. |
US5889159A (en) * | 1993-10-29 | 1999-03-30 | Dana-Farber Cancer Institute, Inc. | Monoclonal antibodies to tumor marker TC1 and methods for use of same |
US5480783A (en) * | 1994-03-31 | 1996-01-02 | The Perkin-Elmer Corporation | Method for reducing background signals in DNA replication/detection assays |
FR2721945B1 (fr) * | 1994-07-04 | 1996-10-18 | David Fabrice | Accroissement genique, un procede d'amplicication genique isotherme et ses applications |
US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
WO1996034983A1 (en) | 1995-05-05 | 1996-11-07 | The Perkin-Elmer Corporation | Methods and reagents for combined pcr amplification and hybridization probing assay |
US5882857A (en) * | 1995-06-07 | 1999-03-16 | Behringwerke Ag | Internal positive controls for nucleic acid amplification |
DE69636838T2 (de) * | 1995-11-25 | 2007-10-18 | Roche Diagnostics Gmbh | Verfahren zur herstellung vielfältiger doppelsträngiger nukleinsäuren |
US6326143B1 (en) | 1996-11-22 | 2001-12-04 | Roche Diagnostics Gmbh | Method for generating multiple double stranded nucleic acids |
US6482590B1 (en) | 1996-12-20 | 2002-11-19 | Aventis Behring Gmbh | Method for polynucleotide amplification |
US6143496A (en) | 1997-04-17 | 2000-11-07 | Cytonix Corporation | Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly |
US8445664B2 (en) | 1998-06-24 | 2013-05-21 | Enzo Diagnostics, Inc. | Kits for amplifying and detecting nucleic acid sequences |
US7315786B2 (en) | 1998-10-16 | 2008-01-01 | Xencor | Protein design automation for protein libraries |
US20020048772A1 (en) | 2000-02-10 | 2002-04-25 | Dahiyat Bassil I. | Protein design automation for protein libraries |
ES2320814T3 (es) * | 1998-11-09 | 2009-05-28 | Eiken Kagaku Kabushiki Kaisha | Procedimiento de sintesis de acido nucleico. |
US6274353B1 (en) * | 1999-09-22 | 2001-08-14 | Genecopoeia, Inc. | Method and compositions for improved polynucleotide synthesis |
JP4721603B2 (ja) | 1999-11-08 | 2011-07-13 | 栄研化学株式会社 | 変異および/または多型の検出方法 |
BR0015382B1 (pt) * | 1999-11-08 | 2014-04-29 | Eiken Chemical | Processos e kits para sintetizacao e amplificação do acido nucleico bem como, processos para detectar uma sequencia de nucleotideo alvo e de uma mutacao da dita sequencia |
AU1057801A (en) * | 1999-11-08 | 2001-06-06 | Eiken Kagaku Kabushiki Kaisha | Method of detecting variation or polymorphism |
US6800439B1 (en) | 2000-01-06 | 2004-10-05 | Affymetrix, Inc. | Methods for improved array preparation |
US6806361B1 (en) | 2000-03-17 | 2004-10-19 | Affymetrix, Inc. | Methods of enhancing functional performance of nucleic acid arrays |
US6833450B1 (en) | 2000-03-17 | 2004-12-21 | Affymetrix, Inc. | Phosphite ester oxidation in nucleic acid array preparation |
AU779160B2 (en) * | 2000-03-28 | 2005-01-06 | Eiken Kagaku Kabushiki Kaisha | Method of synthesizing nucleic acids |
US8017357B2 (en) | 2000-04-07 | 2011-09-13 | Eiken Kagaku Kabushiki Kaisha | Method of amplifying nucleic acid by using double-stranded nucleic acid as template |
US7005259B1 (en) | 2000-06-01 | 2006-02-28 | Affymetrix, Inc. | Methods for array preparation using substrate rotation |
DE10119203B4 (de) * | 2001-04-19 | 2004-09-16 | Rolf Dr. Postina | Verfahren zur Erzeugung von doppelsträngigen Nukleinsäuren mit einzelsträngigen Überhängen beliebiger Länge und Nukleotidzusammensetzung |
EP1503321A3 (en) * | 2001-08-10 | 2006-08-30 | Xencor, Inc. | Protein design automation for protein libraries |
CN1414110A (zh) * | 2002-08-19 | 2003-04-30 | 张旭 | 利用具有3'至5'外切酶活性的多聚酶进行基因序列分析 |
EP1604044B1 (en) * | 2003-03-19 | 2009-05-13 | Discerna Limited | Recovery of dna |
US20070054301A1 (en) * | 2005-09-06 | 2007-03-08 | Gen-Probe Incorporated | Methods, compositions and kits for isothermal amplification of nucleic acids |
CN112159836B (zh) * | 2020-10-09 | 2022-09-06 | 中国科学院长春应用化学研究所 | 一种双发夹连接介导恒温扩增的核酸检测新方法 |
Family Cites Families (34)
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---|---|---|---|---|
US4663283A (en) | 1980-03-24 | 1987-05-05 | Genentech, Inc. | Method of altering double-stranded DNA |
US4358535A (en) | 1980-12-08 | 1982-11-09 | Board Of Regents Of The University Of Washington | Specific DNA probes in diagnostic microbiology |
FI63596C (fi) | 1981-10-16 | 1983-07-11 | Orion Yhtymae Oy | Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser |
US4423153A (en) | 1981-12-03 | 1983-12-27 | Board Of Regents, The University Of Texas System | Methods and compositions for the detection and determination of cellular DNA |
US4480040A (en) | 1981-12-03 | 1984-10-30 | The United States Of America As Represented By The Secretary Of Agriculture | Sensitive and rapid diagnosis of viroid diseases and viruses |
US4656134A (en) | 1982-01-11 | 1987-04-07 | Board Of Trustees Of Leland Stanford Jr. University | Gene amplification in eukaryotic cells |
US4490472A (en) | 1982-06-17 | 1984-12-25 | Imreg, Inc. | Sensitive tests for malignancies based on DNA detection |
US4677054A (en) | 1983-08-08 | 1987-06-30 | Sloan-Kettering Institute For Cancer Research | Method for simple analysis of relative nucleic acid levels in multiple small samples by cytoplasmic dot hybridization |
US4599303A (en) | 1983-12-12 | 1986-07-08 | Hri Associates, Inc. | Nucleic acid hybridization assay employing probes crosslinkable to target sequences |
US4724202A (en) | 1983-12-12 | 1988-02-09 | Molecular Diagnostics, Inc. | Use of non-hybridizable nucleic acids for the detection of nucleic acid hybridization |
US4683194A (en) | 1984-05-29 | 1987-07-28 | Cetus Corporation | Method for detection of polymorphic restriction sites and nucleic acid sequences |
US4647529A (en) | 1984-06-01 | 1987-03-03 | Rodland Karin D | Hybridization method of detecting nucleic acid sequences with probe containing thionucleotide |
US4675283A (en) | 1984-07-19 | 1987-06-23 | Massachusetts Institute Of Technology | Detection and isolation of homologous, repeated and amplified nucleic acid sequences |
US4883750A (en) | 1984-12-13 | 1989-11-28 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
CA1278755C (en) | 1985-03-15 | 1991-01-08 | William J. Knowles | Labelling of oligonucleotides |
US5008182A (en) | 1986-01-10 | 1991-04-16 | Cetus Corporation | Detection of AIDS associated virus by polymerase chain reaction |
ES8706823A1 (es) | 1985-03-28 | 1987-06-16 | Cetus Corp | Un procedimiento para detectar la presencia o ausencia de al menos una secuencia especifica de acidos nucleicos en una muestra |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
GB8515276D0 (en) * | 1985-06-17 | 1985-07-17 | Amersham Int Plc | Nucleic acid sequencing |
US4868104A (en) | 1985-09-06 | 1989-09-19 | Syntex (U.S.A.) Inc. | Homogeneous assay for specific polynucleotides |
US4910300A (en) | 1985-12-11 | 1990-03-20 | Chiron Corporation | Method for making nucleic acid probes |
US4882269A (en) | 1985-12-13 | 1989-11-21 | Princeton University | Amplified hybridization assay |
US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
BE904402A (fr) | 1986-03-12 | 1986-09-12 | Univ Libre De Bruxelles Iribhn | Moule de sonde de detection de sequende d'adn et preparation d'un tel moule et d'une telle sonde. |
US4908307A (en) | 1986-12-19 | 1990-03-13 | Karin D. Rodland | Hybridization method and probe for detecting nucleic acid sequences |
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IL90359A0 (en) | 1988-05-26 | 1989-12-15 | University Patents Inc | Nucleoside and polynucleotide thiophosphoramidite and phosphorodithioate compounds and their production |
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CA2007431A1 (en) | 1989-01-10 | 1990-07-10 | Larry W. Mclauglin | Labeling of nucleic acids with fluorescent markers |
EP0379369B1 (en) | 1989-01-19 | 1996-09-04 | BEHRINGWERKE Aktiengesellschaft | Nucleic acid amplification using single primer |
US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
US5595891A (en) | 1990-07-19 | 1997-01-21 | Behringwerke Ag | Method for producing a polynucleotide for use in single primer amplification |
-
1992
- 1992-10-09 EP EP92309231A patent/EP0549107B1/en not_active Expired - Lifetime
- 1992-10-09 DE DE69223755T patent/DE69223755T2/de not_active Expired - Fee Related
- 1992-10-09 ES ES92309231T patent/ES2112302T3/es not_active Expired - Lifetime
- 1992-10-09 CA CA002080305A patent/CA2080305A1/en not_active Abandoned
- 1992-10-09 AT AT92309231T patent/ATE161586T1/de not_active IP Right Cessation
- 1992-10-09 DK DK92309231T patent/DK0549107T3/da active
- 1992-10-12 JP JP4313917A patent/JPH07143900A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
EP0549107A1 (en) | 1993-06-30 |
EP0549107B1 (en) | 1997-12-29 |
DE69223755T2 (de) | 1998-04-23 |
DK0549107T3 (da) | 1998-08-31 |
JPH07143900A (ja) | 1995-06-06 |
CA2080305A1 (en) | 1993-04-12 |
ATE161586T1 (de) | 1998-01-15 |
DE69223755D1 (de) | 1998-02-05 |
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