ES2112254T3 - Procedimiento de produccion de glicosiltransferasas segregables. - Google Patents

Procedimiento de produccion de glicosiltransferasas segregables.

Info

Publication number
ES2112254T3
ES2112254T3 ES90916656T ES90916656T ES2112254T3 ES 2112254 T3 ES2112254 T3 ES 2112254T3 ES 90916656 T ES90916656 T ES 90916656T ES 90916656 T ES90916656 T ES 90916656T ES 2112254 T3 ES2112254 T3 ES 2112254T3
Authority
ES
Spain
Prior art keywords
soluble
segregable
secretable
production procedure
glycosyltransferases
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90916656T
Other languages
English (en)
Inventor
James C Paulson
Eryn Ujita-Lee
Karen J Colley
Beverly Adler
Jeffrey K Browne
Jasminder Weinstein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
University of California
Original Assignee
Amgen Inc
University of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc, University of California filed Critical Amgen Inc
Application granted granted Critical
Publication of ES2112254T3 publication Critical patent/ES2112254T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

UN METODO PARA LA INGENIERIA GENETICA DE CELULAS PARA PRODUCIR ENZIMAS DE PROCESAMIENTO GOLGI SOLUBLES Y SEGREGABLES EN LUGAR DE ENZIMAS UNIDAS A LA MEMBRANA QUE SURGEN DE MANERA NATURAL. LAS CELULAS PASAN POR UN PROCESO DE INGENIERIA GENETICA PARA EXPRESAR GLICOSILTRANSFERASAS QUE NO TENGAN NI UN ANCLA DE MEMBRANA NI UNA SEÑAL DE RETENCION. LA ENZIMA ALTERADA RESULTANTE ES CONVERTIDA EN SOLUBLE Y SEGREGABLE POR LA CELULA SIN PERDER SU ACTIVIDAD CATALITICA. LA SECRECION DE LA GLICOSILTRANSFERASA SOLUBLE POR LA CELULA PROPORCIONA UNA PRODUCCION MAYOR Y UNA RECUPERACION SIMPLIFICADA DE GLICOSILTRANSFERASA.
ES90916656T 1989-10-24 1990-10-24 Procedimiento de produccion de glicosiltransferasas segregables. Expired - Lifetime ES2112254T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/426,577 US5032519A (en) 1989-10-24 1989-10-24 Method for producing secretable glycosyltransferases and other Golgi processing enzymes

Publications (1)

Publication Number Publication Date
ES2112254T3 true ES2112254T3 (es) 1998-04-01

Family

ID=23691361

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90916656T Expired - Lifetime ES2112254T3 (es) 1989-10-24 1990-10-24 Procedimiento de produccion de glicosiltransferasas segregables.

Country Status (11)

Country Link
US (1) US5032519A (es)
EP (1) EP0497878B1 (es)
JP (1) JP3121342B2 (es)
AT (1) ATE161044T1 (es)
AU (1) AU646233B2 (es)
CA (1) CA2065839C (es)
DE (1) DE69031805T2 (es)
DK (1) DK0497878T3 (es)
ES (1) ES2112254T3 (es)
GR (1) GR3026217T3 (es)
WO (1) WO1991006635A1 (es)

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Also Published As

Publication number Publication date
EP0497878A4 (en) 1993-01-07
EP0497878A1 (en) 1992-08-12
US5032519A (en) 1991-07-16
ATE161044T1 (de) 1997-12-15
CA2065839C (en) 2000-01-11
AU646233B2 (en) 1994-02-17
DE69031805T2 (de) 1998-04-23
WO1991006635A1 (en) 1991-05-16
GR3026217T3 (en) 1998-05-29
AU6648090A (en) 1991-05-31
JP3121342B2 (ja) 2000-12-25
EP0497878B1 (en) 1997-12-10
DE69031805D1 (de) 1998-01-22
JPH05504678A (ja) 1993-07-22
DK0497878T3 (da) 1998-01-12
CA2065839A1 (en) 1991-04-25

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