ES2101116T3 - Metodo de deteccion de una secuencia de acidos nucleicos. - Google Patents

Metodo de deteccion de una secuencia de acidos nucleicos.

Info

Publication number
ES2101116T3
ES2101116T3 ES92919473T ES92919473T ES2101116T3 ES 2101116 T3 ES2101116 T3 ES 2101116T3 ES 92919473 T ES92919473 T ES 92919473T ES 92919473 T ES92919473 T ES 92919473T ES 2101116 T3 ES2101116 T3 ES 2101116T3
Authority
ES
Spain
Prior art keywords
nucleic acid
acid sequence
probes
probe
detection method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92919473T
Other languages
English (en)
Inventor
Donald Leonard Nicholas Cardy
Sabine Yolande Joseph Delnatte
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytocell Ltd
Original Assignee
Cytocell Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB919119650A external-priority patent/GB9119650D0/en
Priority claimed from GB919120656A external-priority patent/GB9120656D0/en
Application filed by Cytocell Ltd filed Critical Cytocell Ltd
Application granted granted Critical
Publication of ES2101116T3 publication Critical patent/ES2101116T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

SE PRESENTA UN METODO PARA ENSAYAR LA PRESENCIA DE UNA SECUENCIA DE ACIDOS NUCLEICOS DE INTERES EN UNA MUESTRA, QUE CONSISTE EN: PONER EN CONTACTO LA MUESTRA CON UNA PRIMERA SONDA Y UNA SEGUNDA SONDA, EN DONDE PORCIONES DE DICHAS SONDAS SON CAPACES DE HIBRIDAR LA SECUENCIA DE INTERES DE TAL MODO QUE LAS SONDAS QUEDEN ADYACENTES O SUSTANCIALMENTE ADYACENTES ENTRE SI, PARA ASI PERMITIR QUE OTRAS PORCIONES DE LA PRIMERA Y SEGUNDA SONDAS SE RECUEZAN ENTRE SI; TRATAR LA MUESTRA PARA PROVOCAR LA EXTENSION EN CADENA DE UNA DE LAS SONDAS UTILIZANDO PARTE DE UNA SONDA COMO PLANTILLA; Y DETECTAR AL MENOS PARTE DE LA SONDA EXTENDIDA.
ES92919473T 1991-09-13 1992-09-14 Metodo de deteccion de una secuencia de acidos nucleicos. Expired - Lifetime ES2101116T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB919119650A GB9119650D0 (en) 1991-07-05 1991-09-13 Homogeneous nucleic acids probe-based test & substances
GB919120656A GB9120656D0 (en) 1991-09-13 1991-09-27 Amplification process

Publications (1)

Publication Number Publication Date
ES2101116T3 true ES2101116T3 (es) 1997-07-01

Family

ID=26299539

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92919473T Expired - Lifetime ES2101116T3 (es) 1991-09-13 1992-09-14 Metodo de deteccion de una secuencia de acidos nucleicos.

Country Status (9)

Country Link
EP (1) EP0666927B1 (es)
JP (1) JP3403405B2 (es)
AT (1) ATE152778T1 (es)
AU (1) AU672367B2 (es)
CA (1) CA2118913C (es)
DE (1) DE69219627T2 (es)
DK (1) DK0666927T3 (es)
ES (1) ES2101116T3 (es)
WO (1) WO1993006240A1 (es)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0652973T3 (da) * 1992-07-31 1997-09-15 Behringwerke Ag Fremgangsmåde til indføring af definerede sekvenser ved 3-enden af polynukleotider
US5846709A (en) * 1993-06-15 1998-12-08 Imclone Systems Incorporated Chemical process for amplifying and detecting nucleic acid sequences
EP0705905B1 (de) * 1994-07-16 2001-10-10 Roche Diagnostics GmbH Verfahren zum sensitiven Nachweis von Nukleinsäuren
DE19610255B4 (de) * 1996-03-15 2004-11-04 Universität Heidelberg Verfahren zur Herstellung von Nukleinsäuresequenzen und Verfahren zum Nachweis von Translokationen zwischen Chromosomen
GB9626074D0 (en) * 1996-12-16 1997-02-05 Cytocell Ltd Nucleic acids amplification assay
CA2218439A1 (en) * 1996-12-21 1998-06-21 Henrik Orum Method of identifying a nucleic acid using triple helix formation of adjacently annealed probes
CA2282705A1 (en) * 1997-02-28 1998-09-03 Exact Laboratories, Inc. Nucleic acid analysis methods
US20030165888A1 (en) 2001-07-18 2003-09-04 Brown Bob D. Oligonucleotide probes and primers comprising universal bases for diagnostic purposes
US6518017B1 (en) 1997-10-02 2003-02-11 Oasis Biosciences Incorporated Combinatorial antisense library
DE69902086T2 (de) * 1998-01-27 2003-03-13 Cytocell Ltd Verfahren zum nachweis von nukleinsäure-zielsequenzen mittels in vitro transkription von einem rna-promoter
CA2319662C (en) * 1998-01-27 2009-11-24 Cytocell Limited Modified nucleic acid probes and uses thereof
US6280947B1 (en) 1999-08-11 2001-08-28 Exact Sciences Corporation Methods for detecting nucleotide insertion or deletion using primer extension
US6503718B2 (en) 1999-01-10 2003-01-07 Exact Sciences Corporation Methods for detecting mutations using primer extension for detecting disease
AU779591B2 (en) * 1999-07-29 2005-02-03 British Biocell International Limited Method for detecting nucleic acid target sequences involving in vitro transcription from an RNA polymerase promoter
GB9917816D0 (en) * 1999-07-29 1999-09-29 Cytocell Ltd Chemically modified probes used to regulate nucleic acid amplification assays
US7005261B1 (en) 1999-07-29 2006-02-28 British Biocell International Limited Method for detecting nucleic acid target sequences involving in vitro transcription from an RNA polymerase promoter
US7306904B2 (en) 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
SE516272C2 (sv) * 2000-02-18 2001-12-10 Ulf Landegren Metoder och kit för analytdetektion mha proximitets-probning
JP2005532827A (ja) * 2002-07-12 2005-11-04 ブリティッシュ・バイオセル・インターナショナル・リミテッド 側方フローアッセイ用のデバイスおよびその方法
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
US9777314B2 (en) 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
GB201101621D0 (en) 2011-01-31 2011-03-16 Olink Ab Method and product
CN105121659A (zh) * 2013-03-15 2015-12-02 富鲁达公司 单细胞中的靶蛋白和靶核酸的同时检测
GB201518655D0 (en) 2015-10-21 2015-12-02 Olink Ab Method for generating proximity probes
GB202403821D0 (en) 2024-03-18 2024-05-01 Biotangents Ltd Probes, primers and methods for nucleic acid detection

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3877279A (en) * 1972-10-30 1975-04-15 Mark Products Corp Van Sheet metal brake
JP3276955B2 (ja) * 1988-09-30 2002-04-22 ジーン−トラック・システムス Ｒｎａの鋳型の末端に結合したプローブ構造およびその使用方法
EP0419081A3 (en) * 1989-09-22 1992-07-22 Microgenics Corporation Method for protein binding enzyme complementation assays
NO904633L (no) * 1989-11-09 1991-05-10 Molecular Diagnostics Inc Amplifikasjon av nukleinsyrer ved transkriberbar haarnaalsprobe.
US5215899A (en) * 1989-11-09 1993-06-01 Miles Inc. Nucleic acid amplification employing ligatable hairpin probe and transcription

Also Published As

Publication number Publication date
CA2118913A1 (en) 1993-04-01
JPH06510669A (ja) 1994-12-01
AU2558692A (en) 1993-04-27
WO1993006240A1 (en) 1993-04-01
EP0666927B1 (en) 1997-05-07
ATE152778T1 (de) 1997-05-15
DE69219627T2 (de) 1997-09-04
JP3403405B2 (ja) 2003-05-06
DK0666927T3 (da) 1997-09-15
DE69219627D1 (de) 1997-06-12
CA2118913C (en) 2008-08-19
EP0666927A1 (en) 1995-08-16
AU672367B2 (en) 1996-10-03

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