ES2081115T3 - Procedimiento inmunologico para la determinacion de anticuerpos en fluidos biologicos asi como kit para realizar el procedimiento. - Google Patents
Procedimiento inmunologico para la determinacion de anticuerpos en fluidos biologicos asi como kit para realizar el procedimiento.Info
- Publication number
- ES2081115T3 ES2081115T3 ES92912568T ES92912568T ES2081115T3 ES 2081115 T3 ES2081115 T3 ES 2081115T3 ES 92912568 T ES92912568 T ES 92912568T ES 92912568 T ES92912568 T ES 92912568T ES 2081115 T3 ES2081115 T3 ES 2081115T3
- Authority
- ES
- Spain
- Prior art keywords
- antibodies
- procedure
- antigen
- kit
- determination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000013060 biological fluid Substances 0.000 title 1
- 230000001900 immune effect Effects 0.000 title 1
- 239000000427 antigen Substances 0.000 abstract 6
- 102000036639 antigens Human genes 0.000 abstract 6
- 108091007433 antigens Proteins 0.000 abstract 6
- 239000003550 marker Substances 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 210000000056 organ Anatomy 0.000 abstract 1
- 239000007790 solid phase Substances 0.000 abstract 1
- 210000001685 thyroid gland Anatomy 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/564—Immunoassay; Biospecific binding assay; Materials therefor for pre-existing immune complex or autoimmune disease, i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Rehabilitation Therapy (AREA)
- Rheumatology (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
PARA DETERMINAR LA PRESENCIA DE ANTICUERPOS (AK), EN PARTICULAR AUTO-ANTICUERPOS TALES COMO AQUELLOS CONTRA PRO-OXIDASA TIROIDES (TPO), LA INVENCION PROPONE QUE LA MUESTRA A CHEQUEAR REACCIONE CON DOS DE OTROS TIPOS DE ANTICUERPOS, EN PARTICULAR ANTICUERPOS MONOCLONALES, Y CON UN ANTIGENO. LOS OTROS DOS TIPOS DE ANTICUERPOS SON UN ANTICUERPO (AKIMM) INMOVILIZADO Y UN ANTICUERPO (AK*) MARCADO DE FORMA ADECUADA CONTRA EL ANTIGENO (AG). EL ANTIGENO ES UTILIZADO PREFERENTEMENTE EN FORMA NATURAL, EN ESTADO BRUTO, POR EJEMPLO COMO EXTRACTO DE UN ORGANO. LA PRESENCIA DEL ANTICUERPO (AK) A SER DETERMINADA ES INDICADA MEDIANTE MODIFICACION DE LA ESTRUCTURA DE UN SANDWICH COMPUESTO DE LOS DOS ANTICUERPOS (AKIMM Y AK*) Y EL ANTIGENO (AG) AÑADIDO, Y POR MEDIO DE LA REDUCCION RESULTANTE EN EL ENLACE DEL MARCADOR DE LA FASE SOLIDA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE4120412A DE4120412C1 (es) | 1991-06-20 | 1991-06-20 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2081115T3 true ES2081115T3 (es) | 1996-02-16 |
Family
ID=6434394
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES92912568T Expired - Lifetime ES2081115T3 (es) | 1991-06-20 | 1992-06-15 | Procedimiento inmunologico para la determinacion de anticuerpos en fluidos biologicos asi como kit para realizar el procedimiento. |
Country Status (9)
Country | Link |
---|---|
US (1) | US5501955A (es) |
EP (1) | EP0544869B2 (es) |
JP (1) | JP2675676B2 (es) |
AT (1) | ATE132976T1 (es) |
CA (1) | CA2089870C (es) |
DE (2) | DE4120412C1 (es) |
DK (1) | DK0544869T3 (es) |
ES (1) | ES2081115T3 (es) |
WO (1) | WO1993000587A1 (es) |
Families Citing this family (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5515705A (en) * | 1992-01-23 | 1996-05-14 | Board Of Regents, The University Of Texas System | Apparatus and method for deforming a workpiece |
DE4243375C2 (de) * | 1992-12-21 | 1998-04-23 | Brahms Diagnostica Gmbh | Bestimmungsverfahren zur Bestimmung von Antikörpern in biologischen Flüssigkeiten |
DE4343255A1 (de) * | 1993-07-13 | 1995-06-22 | Henning Berlin Gmbh | Verfahren zur quantitativen Bestimmung von humaner Thyreoidaler Peroxidase und Kit für die Durchführung des Verfahrens |
DE4323436C2 (de) * | 1993-07-13 | 1995-06-08 | Henning Berlin Gmbh | Verfahren zur quantitativen Bestimmung von humaner Thyreoidaler Peroxidase und Kit für die Durchführung des Verfahrens |
DE4328070C1 (de) * | 1993-08-20 | 1994-11-24 | Henning Berlin Gmbh | Verfahren zur Bestimmung eines Analyten in einem Volumen einer flüssigen Probe sowie seine Anwendung zur Bestimmung von anti-TSH-Rezeptor-Autoantikörpern in einem Patientenserum |
DE19505266C1 (de) * | 1995-02-16 | 1996-10-02 | Brahms Diagnostica Gmbh | Verwendung von polyklonalen humanen anti-hTg-Autoantikörpern als Reagenz für die klinische Diagnostik von Schilddrüsen-Autoimmunerkrankungen sowie Reagenziensatz für eine Bestimmung von anti-hTg-Autoantikörpern in Patientenseren |
DE19527160C1 (de) * | 1995-07-25 | 1997-01-23 | Brahms Diagnostica Gmbh | Verfahren zur Messung der Konzentration eines Enzyms durch photometrische Absorptionsmessung eines enzymatisch gebildeten Farbstoffs in einer Meßlösung |
DE19651093C2 (de) * | 1996-12-09 | 1999-06-10 | Brahms Diagnostica Gmbh | Rezeptorbindungsassay zum Nachweis von TSH-Rezeptor-Autoantikörpern sowie Reagenziensatz für die Durchführung eines solchen Rezeptorbindungsassays |
DE19710211C2 (de) * | 1997-03-12 | 1999-12-16 | Brahms Diagnostica Gmbh | Verfahren zur Bestimmung von Schilddrüsen-Autoantikörpern |
GB9719357D0 (en) * | 1997-09-11 | 1997-11-12 | Ortho Clinical Diagnostics | Immunoassay Utilizing Two Incubations With Labelled Antigen |
GB9810040D0 (en) | 1998-05-11 | 1998-07-08 | Univ Nottingham | Blood borne tumour markers |
GB9823397D0 (en) * | 1998-10-27 | 1998-12-23 | Rsr Ltd | Assays for thyroid autoantibodies |
GB9827228D0 (en) * | 1998-12-10 | 1999-02-03 | Univ Nottingham | Cancer detection method and reagents |
US20030232399A1 (en) * | 2000-06-14 | 2003-12-18 | Robertson John Forsyth Russell | Cancer detection methods and reagents |
GB2424273B (en) * | 2002-11-14 | 2007-06-27 | Univ Nottingham | Method for preparing tumour marker protein |
GB2426581A (en) * | 2005-05-27 | 2006-11-29 | Univ Nottingham | Immunoassay methods |
SI1889059T1 (sl) | 2005-05-27 | 2009-12-31 | Oncimmune Ltd | Izboljšani imunotestni postopek |
US8574848B2 (en) * | 2006-09-13 | 2013-11-05 | Oncimmune Ltd. | Immunoassay methods |
JP5373610B2 (ja) * | 2007-08-06 | 2013-12-18 | 中外製薬株式会社 | リガンド−レセプター結合阻害活性の測定方法 |
GB0725239D0 (en) * | 2007-12-24 | 2008-02-06 | Oncimmune Ltd | Calibrator for autoantibody assay |
JP7153054B2 (ja) * | 2017-07-13 | 2022-10-13 | マグアレイ,インコーポレイテッド | 自己抗体の定量方法 |
CN113156129B (zh) * | 2021-01-13 | 2022-04-05 | 广东菲鹏生物有限公司 | 中和抗体高敏检测方法及产品 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3654090A (en) * | 1968-09-24 | 1972-04-04 | Organon | Method for the determination of antigens and antibodies |
US4469787A (en) * | 1982-05-14 | 1984-09-04 | Mallinckrodt Inc. | Immunoassay involving soluble complex of second antibody and labeled binding protein |
IL73938A (en) * | 1984-01-02 | 1989-09-28 | Boehringer Mannheim Gmbh | Process and reagent for the determination of polyvalent antigen by incubation with three different receptors |
JPS61271457A (ja) * | 1985-05-28 | 1986-12-01 | Olympus Optical Co Ltd | 免疫学的分析方法 |
-
1991
- 1991-06-20 DE DE4120412A patent/DE4120412C1/de not_active Expired - Lifetime
-
1992
- 1992-06-15 DK DK92912568.0T patent/DK0544869T3/da active
- 1992-06-15 US US07/971,822 patent/US5501955A/en not_active Expired - Lifetime
- 1992-06-15 ES ES92912568T patent/ES2081115T3/es not_active Expired - Lifetime
- 1992-06-15 EP EP92912568A patent/EP0544869B2/de not_active Expired - Lifetime
- 1992-06-15 WO PCT/EP1992/001348 patent/WO1993000587A1/de active IP Right Grant
- 1992-06-15 JP JP5501305A patent/JP2675676B2/ja not_active Expired - Lifetime
- 1992-06-15 AT AT92912568T patent/ATE132976T1/de active
- 1992-06-15 CA CA002089870A patent/CA2089870C/en not_active Expired - Lifetime
- 1992-06-15 DE DE59205012T patent/DE59205012D1/de not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
EP0544869B2 (de) | 2001-09-05 |
DE4120412C1 (es) | 1993-01-07 |
DE59205012D1 (de) | 1996-02-22 |
ATE132976T1 (de) | 1996-01-15 |
EP0544869B1 (de) | 1996-01-10 |
WO1993000587A1 (de) | 1993-01-07 |
EP0544869A1 (de) | 1993-06-09 |
US5501955A (en) | 1996-03-26 |
DK0544869T3 (da) | 1996-02-26 |
JPH06501103A (ja) | 1994-01-27 |
JP2675676B2 (ja) | 1997-11-12 |
CA2089870A1 (en) | 1992-12-21 |
CA2089870C (en) | 2003-09-09 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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