ES2079467T3 - Procedimiento y dispositivo para la transformacion genetica de celulas. - Google Patents

Procedimiento y dispositivo para la transformacion genetica de celulas.

Info

Publication number
ES2079467T3
ES2079467T3 ES90810970T ES90810970T ES2079467T3 ES 2079467 T3 ES2079467 T3 ES 2079467T3 ES 90810970 T ES90810970 T ES 90810970T ES 90810970 T ES90810970 T ES 90810970T ES 2079467 T3 ES2079467 T3 ES 2079467T3
Authority
ES
Spain
Prior art keywords
cells
procedure
gold particles
atomised
genetic transformation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90810970T
Other languages
English (en)
Inventor
Christof Dr Sautter
Heinz Waldner
Ingo Prof Dr Potrykus
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Eidgenoessische Technische Hochschule Zurich ETHZ
Original Assignee
Ciba Geigy AG
Eidgenoessische Technische Hochschule Zurich ETHZ
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ciba Geigy AG, Eidgenoessische Technische Hochschule Zurich ETHZ filed Critical Ciba Geigy AG
Application granted granted Critical
Publication of ES2079467T3 publication Critical patent/ES2079467T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Sustainable Development (AREA)
  • Mechanical Engineering (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

LA INVENCION SE REFIERE A LA TRANSFORMACION GENETICA DE CELULAS Y CONSISTE EN; PULVERIZAR UNAS GOTAS (T) DE SOLUCION DE DNA CON PARTICULAS DE ORO SUSPENDIDAS A TRAVES DE PRESIONADORES Y EN LA DESEMBOCADURA DE UNA CANULA (42) PERTENECIENTE A UNA CAMARA DE PRESION TUBULAR (17); PRESIONAR LAS GOTAS QUE LLEVAN LAS PARTICULAS SUSPENDIDAS Y LOS DNA MIGRATORIOS; ACELERAR LAS GOTAS; ENFOCAR LAS GOTAS EN EL EXTREMO DE LA CAMARA DE PRESION A TRAVES DE UNA RESTRICCION (17); HACER PASAR LAS GOTAS A TRAVES DEL ESPACIO LIBRE DE UNA CAMARA (2) DE ENSAYO DE EVACUACION; FIJAR LAS CELULAS EN UNA ZONA PUNTUAL LIMITADORA DE UN SOPORTE (25) Y HACER PENETRAR A LAS GOTAS EN EL SOPORTE.
ES90810970T 1989-12-19 1990-12-11 Procedimiento y dispositivo para la transformacion genetica de celulas. Expired - Lifetime ES2079467T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CH456289 1989-12-19

Publications (1)

Publication Number Publication Date
ES2079467T3 true ES2079467T3 (es) 1996-01-16

Family

ID=4278390

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90810970T Expired - Lifetime ES2079467T3 (es) 1989-12-19 1990-12-11 Procedimiento y dispositivo para la transformacion genetica de celulas.

Country Status (12)

Country Link
US (2) US5976880A (es)
EP (1) EP0434616B1 (es)
JP (1) JPH0568575A (es)
AT (1) ATE130371T1 (es)
AU (1) AU636422B2 (es)
CA (1) CA2032443A1 (es)
DE (1) DE59009881D1 (es)
ES (1) ES2079467T3 (es)
HU (1) HUT56141A (es)
IE (1) IE904582A1 (es)
IL (1) IL96689A0 (es)
NZ (1) NZ236486A (es)

Families Citing this family (82)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5550318A (en) * 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6803499B1 (en) 1989-08-09 2004-10-12 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
JPH0376568A (ja) * 1989-08-16 1991-04-02 Minoru Igari 細胞処理用噴射装置及び同装置のピストン
US6777589B1 (en) 1990-01-22 2004-08-17 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6946587B1 (en) * 1990-01-22 2005-09-20 Dekalb Genetics Corporation Method for preparing fertile transgenic corn plants
US6329574B1 (en) 1990-01-22 2001-12-11 Dekalb Genetics Corporation High lysine fertile transgenic corn plants
US6025545A (en) 1990-01-22 2000-02-15 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US6395966B1 (en) 1990-08-09 2002-05-28 Dekalb Genetics Corp. Fertile transgenic maize plants containing a gene encoding the pat protein
AU2781892A (en) * 1991-10-07 1993-05-03 Ciba-Geigy Ag Particle gun for introducing dna into intact cells
US6118047A (en) 1993-08-25 2000-09-12 Dekalb Genetic Corporation Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction
US6326527B1 (en) 1993-08-25 2001-12-04 Dekalb Genetics Corporation Method for altering the nutritional content of plant seed
US6433154B1 (en) * 1997-06-12 2002-08-13 Bristol-Myers Squibb Company Functional receptor/kinase chimera in yeast cells
US6586661B1 (en) 1997-06-12 2003-07-01 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid
US6284345B1 (en) 1997-12-08 2001-09-04 Washington University Designer particles of micron and submicron dimension
US6153811A (en) * 1997-12-22 2000-11-28 Dekalb Genetics Corporation Method for reduction of transgene copy number
US6307123B1 (en) 1998-05-18 2001-10-23 Dekalb Genetics Corporation Methods and compositions for transgene identification
GB9816138D0 (en) 1998-07-24 1998-09-23 Ciba Geigy Ag Organic compounds
DE19926216A1 (de) * 1999-06-09 2001-02-22 Metallgesellschaft Ag Verfahren zur Herstellung von Bariumsulfat, Bariumsulfat und Verwendung des Bariumsulfats
AU1803601A (en) * 1999-11-29 2001-06-04 Midwest Oilseeds, Inc. Methods and compositions for the introduction of molecules into cells
US7314969B2 (en) 1999-11-29 2008-01-01 Midwest Oilseeds, Inc. Methods and compositions for the introduction of molecules into cells
GB0002814D0 (en) 2000-02-09 2000-03-29 Univ York Nucleic acids and their uses
GB0008494D0 (en) 2000-04-07 2000-05-24 Secr Defence Microprojectile delivery system
WO2001087491A1 (en) 2000-05-16 2001-11-22 Regents Of The University Of Minnesota High mass throughput particle generation using multiple nozzle spraying
WO2002018607A2 (en) 2000-08-30 2002-03-07 North Carolina State University Transgenic plants containing molecular decoys that alter protein content therein
JP2004533807A (ja) * 2000-11-07 2004-11-11 ノース・キャロライナ・ステイト・ユニヴァーシティ プトレッシン−ｎ−メチルトランスフェラーゼプロモーター
CA2429272C (en) * 2000-11-28 2016-06-28 Midwest Oilseeds, Inc. Methods and compositions for the introduction of molecules into cells
SG135907A1 (en) 2001-03-15 2007-10-29 Sumitomo Chemical Co Analysis of agonist-activity and antagonist-activity to cytokinin receptor
US7247338B2 (en) 2001-05-16 2007-07-24 Regents Of The University Of Minnesota Coating medical devices
IL159213A0 (en) * 2001-06-08 2004-06-01 Vector Tobacco Ltd Modifying nicotine and nitrosamine levels in tobacco
US20060157072A1 (en) * 2001-06-08 2006-07-20 Anthony Albino Method of reducing the harmful effects of orally or transdermally delivered nicotine
WO2003040370A1 (fr) 2001-10-19 2003-05-15 Sumitomo Chemical Company, Limited Proteines du metabolisme permettant de lutter contre les mauvaises herbes, leurs genes et leur utilisation
EP1499188A4 (en) * 2002-04-09 2007-11-14 Vector Tobacco Ltd TOBACCO WITH A REDUCED NICOTINE AND NITROSAMINE CONTENT
US20060005282A1 (en) * 2002-04-23 2006-01-05 Activx Biosciences, Inc Production and use of salt tolerant and culture density tolerant organisms
CA2492917C (en) 2002-07-19 2011-10-18 University Of South Carolina Compositions and methods for the modulation of gene expression in plants
WO2004013333A2 (de) 2002-07-26 2004-02-12 Basf Plant Science Gmbh Revertierung der negativ-selektiven wirkung von negativen markerproteinen als selektionsverfahren
EP1502954A1 (en) 2003-07-31 2005-02-02 greenovation Biotech GmbH Utilisation of constructs comprising recombination sequence motifes for enhancing gene expression in moss
ES2346224T4 (es) 2003-11-10 2011-06-01 Icon Genetics Gmbh Sistema de expresión de planta derivado de virus de arn.
EP1616959A1 (en) 2004-07-07 2006-01-18 Icon Genetics AG Biological safe transient protein expression in plants
US7957507B2 (en) 2005-02-28 2011-06-07 Cadman Patrick F Method and apparatus for modulating a radiation beam
US8232535B2 (en) 2005-05-10 2012-07-31 Tomotherapy Incorporated System and method of treating a patient with radiation therapy
US8442287B2 (en) 2005-07-22 2013-05-14 Tomotherapy Incorporated Method and system for evaluating quality assurance criteria in delivery of a treatment plan
CA2616136A1 (en) * 2005-07-22 2007-02-01 Tomotherapy Incorporated System and method of evaluating dose delivered by a radiation therapy system
CA2616301A1 (en) * 2005-07-22 2007-02-01 Tomotherapy Incorporated Method and system for evaluating delivered dose
CA2616299A1 (en) * 2005-07-22 2007-02-01 Tomotherapy Incorporated Method of placing constraints on a deformation map and system for implementing same
JP2009502250A (ja) * 2005-07-22 2009-01-29 トモセラピー・インコーポレーテッド 放射線療法治療計画に関連するデータを処理するための方法およびシステム
KR20080044247A (ko) * 2005-07-22 2008-05-20 토모테라피 인코포레이티드 의료 기기의 동작을 모니터링하는 시스템 및 방법
ATE507879T1 (de) * 2005-07-22 2011-05-15 Tomotherapy Inc System zur verabreichung einer strahlentherapie auf ein sich bewegendes zielgebiet
KR20080049716A (ko) 2005-07-22 2008-06-04 토모테라피 인코포레이티드 치료 계획의 전달과 관련된 퀄리티 보증 기준을 평가하는방법 및 시스템
CA2616304A1 (en) * 2005-07-22 2007-02-01 Tomotherapy Incorporated System and method of delivering radiation therapy to a moving region of interest
CN101267858A (zh) * 2005-07-22 2008-09-17 断层放疗公司 根据生物学模型修改放射疗法治疗计划的方法和***
WO2007014090A2 (en) 2005-07-23 2007-02-01 Tomotherapy Incorporated Radiation therapy imaging and delivery utilizing coordinated motion of gantry and couch
US20070164133A1 (en) * 2006-01-18 2007-07-19 Hao-Jan Lin Low pressure gas accelerated gene gun
US20070173470A1 (en) * 2006-01-23 2007-07-26 Chi-Hung Lin Methods for delivering extracellular target into cells
US9248217B2 (en) 2006-01-31 2016-02-02 Nanocopocia, LLC Nanoparticle coating of surfaces
CA2637883C (en) 2006-01-31 2015-07-07 Regents Of The University Of Minnesota Electrospray coating of objects
US9108217B2 (en) 2006-01-31 2015-08-18 Nanocopoeia, Inc. Nanoparticle coating of surfaces
US9040816B2 (en) 2006-12-08 2015-05-26 Nanocopoeia, Inc. Methods and apparatus for forming photovoltaic cells using electrospray
US8153863B2 (en) 2007-03-23 2012-04-10 New York University Transgenic plants expressing GLK1 and CCA1 having increased nitrogen assimilation capacity
GB0709835D0 (en) 2007-05-22 2007-07-04 Plant Bioscience Ltd Composition and method for modulating plant root hair development
US8642847B2 (en) * 2007-06-21 2014-02-04 Lee K. French Corn inbreds like FAR601 and hybrids thereof
US8945653B2 (en) * 2007-06-21 2015-02-03 Suntava, Llc Extracted whole corn kernels and improved processed and processable corn produced thereby
GB2465749B (en) 2008-11-25 2013-05-08 Algentech Sas Plant cell transformation method
GB2465748B (en) 2008-11-25 2012-04-25 Algentech Sas Plant cell transformation method
GB2467167B (en) 2009-01-26 2013-09-11 Algentech Sas Gene targeting in plants
US20120174263A1 (en) 2009-06-15 2012-07-05 Plant Bioscience Limited Production of viral capsids
US20120131701A1 (en) 2009-08-05 2012-05-24 Chromocell Corporation Improved plants, microbes, and organisms
EP2357239A1 (en) 2009-10-29 2011-08-17 Universität zu Köln Methods and means for a selectable marker system in plants
GB201008720D0 (en) 2010-05-25 2010-07-07 Univ Aberdeen RxLR-leader peptides and protein translocation
WO2012095841A1 (en) 2011-01-10 2012-07-19 State Of Israel, Ministry Of Agriculture And Rural Development, A.R.O. - Volcani Center Improved tomato plants
EP2820037B1 (en) 2012-02-29 2020-02-19 Syngenta Participations AG Modulation of seed vigor
US20140123339A1 (en) 2012-10-31 2014-05-01 Pioneer Hi Bred International Inc Transformed Plants Having Increased Beta-Carotene Levels, Increased Half-Life and Bioavailability and Methods of Producing Such
CN105027227B (zh) 2013-02-26 2017-09-08 安科锐公司 电磁致动的多叶准直器
WO2014147235A1 (en) 2013-03-22 2014-09-25 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Kits comprising plus-sense single stranded rna viral vectors and methods for producing polypeptides using the kits
UA119645C2 (uk) 2013-08-14 2019-07-25 Інстітьют Оф Дженетікс Енд Девелопментал Біолоджи Спосіб підвищення врожайності рослин
GB201319876D0 (en) 2013-11-11 2013-12-25 Plant Bioscience Ltd Methods of modulating seed and organ size in plants
CA2965509C (en) * 2014-10-24 2023-03-14 Avectas Limited Delivery across cell plasma membranes
JP7449646B2 (ja) 2015-12-30 2024-03-14 アヴェクタス リミテッド 細胞および組織への遺伝子編集タンパク質および組成物の、ベクターなしでの送達
EP3260542A1 (en) 2016-06-20 2017-12-27 Algentech Protein production in plant cells
US20200332243A1 (en) * 2017-12-20 2020-10-22 Sqz Biotechnologies Company System for delivery of a payload into a cell
WO2019185129A1 (en) 2018-03-27 2019-10-03 The University Court Of The University Of Glasgow Bacterial pathogen derived resistance in plants
CA3180301A1 (en) * 2020-05-26 2021-12-02 Otto J. Prohaska Devices and methods for transfection

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) * 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
IL84459A (en) * 1986-12-05 1993-07-08 Agracetus Apparatus and method for the injection of carrier particles carrying genetic material into living cells
US5015580A (en) * 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
US5141131A (en) * 1989-06-30 1992-08-25 Dowelanco Method and apparatus for the acceleration of a propellable matter
WO1991000915A1 (en) * 1989-07-11 1991-01-24 Biotechnology Research & Development Corporation Aerosol beam microinjector
US5240842A (en) * 1989-07-11 1993-08-31 Biotechnology Research And Development Corporation Aerosol beam microinjector
JPH0376568A (ja) * 1989-08-16 1991-04-02 Minoru Igari 細胞処理用噴射装置及び同装置のピストン

Also Published As

Publication number Publication date
IL96689A0 (en) 1991-09-16
JPH0568575A (ja) 1993-03-23
CA2032443A1 (en) 1991-06-20
US5976880A (en) 1999-11-02
ATE130371T1 (de) 1995-12-15
AU6818290A (en) 1991-06-27
EP0434616A1 (de) 1991-06-26
DE59009881D1 (de) 1995-12-21
US5877023A (en) 1999-03-02
EP0434616B1 (de) 1995-11-15
IE904582A1 (en) 1991-06-19
HUT56141A (en) 1991-07-29
AU636422B2 (en) 1993-04-29
NZ236486A (en) 1992-04-28

Similar Documents

Publication Publication Date Title
ES2079467T3 (es) Procedimiento y dispositivo para la transformacion genetica de celulas.
US4877745A (en) Apparatus and process for reagent fluid dispensing and printing
CA1308467C (en) Apparatus and process for reagent fluid dispensing and printing
EP1180135B1 (en) Apparatus and method for cell disruption
US9943848B2 (en) Apparatus and method for cell disruption
US5180065A (en) Apparatus for and method of fractionating particle in particle-suspended liquid in conformity with the properties thereof
US7392908B2 (en) Methods and apparatus for sorting particles hydraulically
AU3154797A (en) System and methods for monitoring for dna amplification by fluorescence
JPH0640061B2 (ja) フローサイトメータ用の捕獲管分類装置及びその方法
EP0202937A3 (en) Surface analysis spectroscopy apparatus
US20060027686A1 (en) Apparatus and method for cell disruption
WO1997029355A1 (de) Verfahren und vorrichtung zur berührungslosen mikroinjektion sowie zum sortieren und zur gewinnung von planar ausgebrachten biologischen objekten mit laserstrahlen
CN110333282B (zh) 一种流式单细胞质谱分析仪及其使用方法
EP1876451A3 (en) Handling method of body fluid sample and analysis apparatus using the same
EP0881482A3 (en) High duty cycle assembly for detecting molecules
CA2042408A1 (fr) Instrument pour la manutention de biopsies et de prelevements
CA2212472A1 (en) Apparatus for effective collection of explosive and narcotic samples
Telford Flow cytometry and cell sorting
JP2644795B2 (ja) 凍結試料採取方法及び装置
D'Arena et al. Circulating hematopoietic progenitor cells in leukapheresis: flow cytometric estimation
ES2050369T3 (es) Pistola de particulas mejorada.
JPH04204254A (ja) セルソータ
JPH0448245A (ja) 粒子測定装置
JPH03251187A (ja) 生物または細胞に異物を導入し取り込ませる方法および該方法に使用する装置
RU1778631C (ru) Устройство многофракционной сортировки клеток, хромосом и микрочастиц

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 434616

Country of ref document: ES