ES2073451T3 - Vectores de clonacion y de expresion en una cepa de actinomiceto, procedimiento de transformacion de dicha cepa, cepa de actinomiceto obtenida y preparacion de proteinas. - Google Patents
Vectores de clonacion y de expresion en una cepa de actinomiceto, procedimiento de transformacion de dicha cepa, cepa de actinomiceto obtenida y preparacion de proteinas.Info
- Publication number
- ES2073451T3 ES2073451T3 ES89401347T ES89401347T ES2073451T3 ES 2073451 T3 ES2073451 T3 ES 2073451T3 ES 89401347 T ES89401347 T ES 89401347T ES 89401347 T ES89401347 T ES 89401347T ES 2073451 T3 ES2073451 T3 ES 2073451T3
- Authority
- ES
- Spain
- Prior art keywords
- strain
- expression
- cloning
- actinomicet
- actinomicate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Saccharide Compounds (AREA)
Abstract
VECTOR DE CLONAJE Y DE EXPRESION DE UNA SECUENCIA DE ADN CODIFICADA PARA UNA PROTEINA DETERMINADA EN UNA CEPA DE ACTIOMICETE. SE CARACTERIZA POR TENER: UNA SECUENCIA DE ENLACE ATT; - UNA SECUENCIA DE ADN CODIFICADA PARA UNA SECUENCIA INT FUNCIONAL EN DICHA CEPA; Y - UNA SECUENCIA DE ADN CODIFICADA PARA DICHA PROTEINA ESPECIFICA Y PARA LOS ELEMENTOS QUE ASEGURAN LA EXPRESION DE DICHA SECUENCIA EN DICHA CEPA.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR8806638A FR2631634B1 (fr) | 1988-05-18 | 1988-05-18 | Vecteurs de clonage et d'expression dans une souche d'actinomycete, procede de transformation de cette souche, souche d'actynomycete obtenue et preparation de proteines |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2073451T3 true ES2073451T3 (es) | 1995-08-16 |
Family
ID=9366396
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES89401347T Expired - Lifetime ES2073451T3 (es) | 1988-05-18 | 1989-05-16 | Vectores de clonacion y de expresion en una cepa de actinomiceto, procedimiento de transformacion de dicha cepa, cepa de actinomiceto obtenida y preparacion de proteinas. |
Country Status (8)
Country | Link |
---|---|
US (1) | US5688689A (es) |
EP (1) | EP0350341B1 (es) |
JP (1) | JP2913406B2 (es) |
AT (1) | ATE122399T1 (es) |
CA (1) | CA1340746C (es) |
DE (1) | DE68922533T2 (es) |
ES (1) | ES2073451T3 (es) |
FR (1) | FR2631634B1 (es) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2713658B1 (fr) * | 1993-12-08 | 1996-04-12 | Rhone Poulenc Rorer Sa | Séquences d'acides nucléiques régulatrices et utilisations. |
US6825012B2 (en) | 1995-02-23 | 2004-11-30 | Gencell S.A. | DNA molecules, preparation and use in gene therapy |
FR2731014B1 (fr) * | 1995-02-23 | 1997-03-28 | Rhone Poulenc Rorer Sa | Molecules d'adn, preparation et utilisation en therapie genique |
US7255989B1 (en) | 1999-11-29 | 2007-08-14 | Aventis Pharma S.A. | Method for obtaining nucleic acids from an environment sample, resulting nucleic acids and use in synthesis of novel compounds |
US6861513B2 (en) | 2000-01-12 | 2005-03-01 | Schering Corporation | Everninomicin biosynthetic genes |
US7220567B2 (en) | 2000-05-17 | 2007-05-22 | Schering Corporation | Isolation of Micromonospora carbonacea var africana pMLP1 integrase and use of integrating function for site-specific integration into Micromonospora halophitica and Micromonospora carbonacea chromosome |
FR2921663A1 (fr) | 2007-10-02 | 2009-04-03 | Bluestar Silicones France Soc | Polyorganosiloxanes a fonction piperidine depourvus de toxicite par contact cutane et utilisation de ces derniers dans des compositions cosmetiques |
DE102011118018B4 (de) | 2011-10-25 | 2017-10-26 | Plasmidfactory Gmbh & Co. Kg | Minicircles mit Transpositionskassetten und ihre Verwendung zur Transformation von Zellen |
DE102013220859B4 (de) | 2013-10-15 | 2016-09-08 | Plasmidfactory Gmbh & Co. Kg | Minicircles mit viralen Expressionskassetten und ihre Verwendung zur Transformation von Zellen zur Erzeugung rekombinanter Viren oder viraler Genvektoren |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4743546A (en) * | 1985-02-13 | 1988-05-10 | Biotechnica International, Inc. | Controlled gene excision |
AU604475B2 (en) * | 1986-02-28 | 1990-12-20 | Smithkline Beckman Corporation | The gal operon streptomyces |
DE3614310A1 (de) * | 1986-04-28 | 1987-10-29 | Hoechst Ag | Verfahren zur isolierung mutierter gene sowie der entsprechenden wildtyp-gene |
-
1988
- 1988-05-18 FR FR8806638A patent/FR2631634B1/fr not_active Expired - Fee Related
-
1989
- 1989-05-15 CA CA000599737A patent/CA1340746C/fr not_active Expired - Lifetime
- 1989-05-16 ES ES89401347T patent/ES2073451T3/es not_active Expired - Lifetime
- 1989-05-16 DE DE68922533T patent/DE68922533T2/de not_active Expired - Lifetime
- 1989-05-16 JP JP1122704A patent/JP2913406B2/ja not_active Expired - Lifetime
- 1989-05-16 EP EP89401347A patent/EP0350341B1/fr not_active Expired - Lifetime
- 1989-05-16 AT AT89401347T patent/ATE122399T1/de not_active IP Right Cessation
-
1995
- 1995-06-07 US US08/477,502 patent/US5688689A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
DE68922533D1 (de) | 1995-06-14 |
DE68922533T2 (de) | 1995-10-19 |
FR2631634A1 (fr) | 1989-11-24 |
FR2631634B1 (fr) | 1991-06-14 |
EP0350341A1 (fr) | 1990-01-10 |
ATE122399T1 (de) | 1995-05-15 |
EP0350341B1 (fr) | 1995-05-10 |
CA1340746C (fr) | 1999-09-14 |
JP2913406B2 (ja) | 1999-06-28 |
JPH0269185A (ja) | 1990-03-08 |
US5688689A (en) | 1997-11-18 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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