ES2068279T3 - Procedimiento modificado del fosforoamidito para la preparacion de acidos nucleicos modificados. - Google Patents
Procedimiento modificado del fosforoamidito para la preparacion de acidos nucleicos modificados.Info
- Publication number
- ES2068279T3 ES2068279T3 ES90109092T ES90109092T ES2068279T3 ES 2068279 T3 ES2068279 T3 ES 2068279T3 ES 90109092 T ES90109092 T ES 90109092T ES 90109092 T ES90109092 T ES 90109092T ES 2068279 T3 ES2068279 T3 ES 2068279T3
- Authority
- ES
- Spain
- Prior art keywords
- modified
- preparation
- nucleic acids
- phosphoramidite procedure
- phosphoramidite
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title abstract 2
- 150000008300 phosphoramidites Chemical class 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000002777 nucleoside Substances 0.000 abstract 1
- -1 nucleoside phosphoramidite Chemical class 0.000 abstract 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/22—Amides of acids of phosphorus
- C07F9/24—Esteramides
- C07F9/2454—Esteramides the amide moiety containing a substituent or a structure which is considered as characteristic
- C07F9/2458—Esteramides the amide moiety containing a substituent or a structure which is considered as characteristic of aliphatic amines
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/22—Amides of acids of phosphorus
- C07F9/26—Amides of acids of phosphorus containing P-halide groups
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
- C07F9/6558—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system
- C07F9/65586—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing at least two different or differently substituted hetero rings neither condensed among themselves nor condensed with a common carbocyclic ring or ring system at least one of the hetero rings does not contain nitrogen as ring hetero atom
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
- C07F9/6561—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing systems of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring or ring system, with or without other non-condensed hetero rings
- C07F9/65616—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom containing systems of two or more relevant hetero rings condensed among themselves or condensed with a common carbocyclic ring or ring system, with or without other non-condensed hetero rings containing the ring system having three or more than three double bonds between ring members or between ring members and non-ring members, e.g. purine or analogs
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Luminescent Compositions (AREA)
Abstract
EL INVENTO SE REFIERE A UN PROCEDIMIENTO DE MODIFICACION DE FOSFORAMIDITA Y DESCRIBE LA APLICACION DE LA FOSFORAMIDITA MODIFICADA EN LA SINTESIS DE SECUENCIAS NUCLEOTIDAS. EL PROCEDIMIENTO SE CARACTERIZA PORQUE EMPLEANDO UNAS NUCLEOSIDASFOSFORAMIDITAS MODIFICADAS SE POSIBILITA LA PRODUCCION DE SECUENCIAS NUCLEOTIDAS QUE PRESENTAN RESTOS DE FOSFATOS MODIFICADOS.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE3916871A DE3916871A1 (de) | 1989-05-24 | 1989-05-24 | Modifiziertes phosphoramidit-verfahren zur herstellung von modifizierten nukleinsaeuren |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2068279T3 true ES2068279T3 (es) | 1995-04-16 |
Family
ID=6381283
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES90109092T Expired - Lifetime ES2068279T3 (es) | 1989-05-24 | 1990-05-15 | Procedimiento modificado del fosforoamidito para la preparacion de acidos nucleicos modificados. |
Country Status (11)
Country | Link |
---|---|
US (2) | US5700919A (es) |
EP (1) | EP0399330B1 (es) |
JP (2) | JPH0730108B2 (es) |
KR (2) | KR920007274B1 (es) |
AT (1) | ATE116327T1 (es) |
CA (1) | CA2017369C (es) |
DE (2) | DE3916871A1 (es) |
DK (1) | DK0399330T3 (es) |
ES (1) | ES2068279T3 (es) |
GR (1) | GR3015223T3 (es) |
ZA (1) | ZA903975B (es) |
Families Citing this family (58)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3916871A1 (de) * | 1989-05-24 | 1990-11-29 | Boehringer Mannheim Gmbh | Modifiziertes phosphoramidit-verfahren zur herstellung von modifizierten nukleinsaeuren |
US5859221A (en) * | 1990-01-11 | 1999-01-12 | Isis Pharmaceuticals, Inc. | 2'-modified oligonucleotides |
US5670633A (en) * | 1990-01-11 | 1997-09-23 | Isis Pharmaceuticals, Inc. | Sugar modified oligonucleotides that detect and modulate gene expression |
US6399754B1 (en) | 1991-12-24 | 2002-06-04 | Isis Pharmaceuticals, Inc. | Sugar modified oligonucleotides |
US5623065A (en) * | 1990-08-13 | 1997-04-22 | Isis Pharmaceuticals, Inc. | Gapped 2' modified oligonucleotides |
US6005087A (en) | 1995-06-06 | 1999-12-21 | Isis Pharmaceuticals, Inc. | 2'-modified oligonucleotides |
US5872232A (en) * | 1990-01-11 | 1999-02-16 | Isis Pharmaceuticals Inc. | 2'-O-modified oligonucleotides |
US7101993B1 (en) | 1990-01-11 | 2006-09-05 | Isis Pharmaceuticals, Inc. | Oligonucleotides containing 2′-O-modified purines |
US5914396A (en) * | 1990-01-11 | 1999-06-22 | Isis Pharmaceuticals, Inc. | 2'-O-modified nucleosides and phosphoramidites |
US5955589A (en) * | 1991-12-24 | 1999-09-21 | Isis Pharmaceuticals Inc. | Gapped 2' modified oligonucleotides |
US5391785A (en) * | 1990-01-16 | 1995-02-21 | La Jolla Pharmaceutial Company | Intermediates for providing functional groups on the 5' end of oligonucleotides |
US5965722A (en) * | 1991-05-21 | 1999-10-12 | Isis Pharmaceuticals, Inc. | Antisense inhibition of ras gene with chimeric and alternating oligonucleotides |
US7015315B1 (en) | 1991-12-24 | 2006-03-21 | Isis Pharmaceuticals, Inc. | Gapped oligonucleotides |
JP2899111B2 (ja) * | 1991-07-15 | 1999-06-02 | ラ ホヤ ファーマシューティカル カンパニー | オリゴヌクレオチドの5’末端へ官能基を提供するための修飾された亜リン酸中間体 |
US7119184B2 (en) | 1991-08-12 | 2006-10-10 | Isis Pharmaceuticals, Inc. | Oligonucleotides having A-DNA form and B-DNA form conformational geometry |
US6307040B1 (en) | 1992-03-05 | 2001-10-23 | Isis Pharmaceuticals, Inc. | Sugar modified oligonucleotides that detect and modulate gene expression |
IT1249732B (it) * | 1991-11-26 | 1995-03-09 | Angeletti P Ist Richerche Bio | Oligonucleotidi antisenso. |
ATE515510T1 (de) * | 1991-12-24 | 2011-07-15 | Isis Pharmaceuticals Inc | Durch dna-abschnitte unterbrochene modifizierte oligonukleotide |
US6277603B1 (en) | 1991-12-24 | 2001-08-21 | Isis Pharmaceuticals, Inc. | PNA-DNA-PNA chimeric macromolecules |
US5856455A (en) * | 1991-12-24 | 1999-01-05 | Isis Pharmaceuticals, Inc. | Gapped 2'-modified oligonucleotides |
US5700922A (en) * | 1991-12-24 | 1997-12-23 | Isis Pharmaceuticals, Inc. | PNA-DNA-PNA chimeric macromolecules |
US6346614B1 (en) | 1992-07-23 | 2002-02-12 | Hybridon, Inc. | Hybrid oligonucleotide phosphorothioates |
TW244371B (es) * | 1992-07-23 | 1995-04-01 | Tri Clover Inc | |
US5652355A (en) * | 1992-07-23 | 1997-07-29 | Worcester Foundation For Experimental Biology | Hybrid oligonucleotide phosphorothioates |
EP0593901B2 (de) | 1992-09-24 | 2008-04-09 | Sanofi-Aventis Deutschland GmbH | Oligoribonucleotid- und Ribozym-Analoga mit terminalen 3'-3'-bzw.5'-5'-Verknüpfungen |
DE4239531A1 (de) * | 1992-11-25 | 1994-05-26 | Gabor Dr Igloi | Reagenz zur Kupplung verschiedener Substanzen an Nukleinsäuren sowie Verfahren zu dessen Herstellung |
KR100361933B1 (ko) | 1993-09-08 | 2003-02-14 | 라 졸라 파마슈티칼 컴파니 | 화학적으로정의된비중합성결합가플랫폼분자및그것의콘주게이트 |
DE4418691A1 (de) * | 1994-05-28 | 1996-02-22 | Boehringer Mannheim Gmbh | 3'-(4'-) nicht-radioaktiv markierte Nukleoside und Nukleotide mit Aminocarbonsäure-, Peptid- oder Carbonsäure-Spacer |
SE9500342D0 (sv) | 1995-01-31 | 1995-01-31 | Marek Kwiatkowski | Novel chain terminators, the use thereof for nucleic acid sequencing and synthesis and a method of their preparation |
US6017700A (en) * | 1995-08-04 | 2000-01-25 | Bayer Corporation | Cationic oligonucleotides, and related methods of synthesis and use |
US5856099A (en) * | 1996-05-21 | 1999-01-05 | Isis Pharmaceuticals, Inc. | Antisense compositions and methods for modulating type I interleukin-1 receptor expression |
US5898031A (en) | 1996-06-06 | 1999-04-27 | Isis Pharmaceuticals, Inc. | Oligoribonucleotides for cleaving RNA |
US7812149B2 (en) | 1996-06-06 | 2010-10-12 | Isis Pharmaceuticals, Inc. | 2′-Fluoro substituted oligomeric compounds and compositions for use in gene modulations |
US9096636B2 (en) | 1996-06-06 | 2015-08-04 | Isis Pharmaceuticals, Inc. | Chimeric oligomeric compounds and their use in gene modulation |
DE19637042A1 (de) * | 1996-09-12 | 1998-03-19 | Boehringer Mannheim Gmbh | Heterocyclische Verbindungen und deren Verwendung in der Detektion von Nucleinsäuren |
US6576752B1 (en) | 1997-02-14 | 2003-06-10 | Isis Pharmaceuticals, Inc. | Aminooxy functionalized oligomers |
US6172209B1 (en) | 1997-02-14 | 2001-01-09 | Isis Pharmaceuticals Inc. | Aminooxy-modified oligonucleotides and methods for making same |
US6127533A (en) | 1997-02-14 | 2000-10-03 | Isis Pharmaceuticals, Inc. | 2'-O-aminooxy-modified oligonucleotides |
US6184347B1 (en) | 1998-11-19 | 2001-02-06 | Agilent Technologies Inc. | Minimization of blooming in high-density arrays by using reactive wash reagents |
CA2370478A1 (en) | 1999-03-24 | 2000-09-28 | Serge L. Beaucage | N-acylphosphoramidites and their use in oligonucleotide synthesis |
US6306599B1 (en) | 1999-07-16 | 2001-10-23 | Agilent Technologies Inc. | Biopolymer arrays and their fabrication |
CN1434726A (zh) * | 2000-06-08 | 2003-08-06 | 拉卓拉药物公司 | 包含高分子量聚环氧乙烷的多价平台分子 |
EP1176151B1 (en) | 2000-07-28 | 2014-08-20 | Agilent Technologies, Inc. | Synthesis of polynucleotides using combined oxidation/deprotection chemistry |
US6693187B1 (en) * | 2000-10-17 | 2004-02-17 | Lievre Cornu Llc | Phosphinoamidite carboxlates and analogs thereof in the synthesis of oligonucleotides having reduced internucleotide charge |
US6932943B1 (en) | 2001-01-26 | 2005-08-23 | Third Wave Technologies | Nucleic acid synthesizers |
US7435390B2 (en) * | 2001-01-26 | 2008-10-14 | Third Wave Technologies, Inc. | Nucleic acid synthesizers |
DE10133779A1 (de) * | 2001-07-16 | 2003-02-06 | Chemogenix Gmbh | Multimerpolynukleotidsynthese |
AU2002353001A1 (en) * | 2001-12-03 | 2003-06-17 | The Government Of The United States Of America, Represented By The Secretary Of The Department Of He | Thermolabile hydroxyl protecting groups and methods of use |
AU2003291755A1 (en) | 2002-11-05 | 2004-06-07 | Isis Pharmaceuticals, Inc. | Oligomers comprising modified bases for binding cytosine and uracil or thymine and their use |
US8569474B2 (en) | 2004-03-09 | 2013-10-29 | Isis Pharmaceuticals, Inc. | Double stranded constructs comprising one or more short strands hybridized to a longer strand |
US8394947B2 (en) | 2004-06-03 | 2013-03-12 | Isis Pharmaceuticals, Inc. | Positionally modified siRNA constructs |
US7884086B2 (en) | 2004-09-08 | 2011-02-08 | Isis Pharmaceuticals, Inc. | Conjugates for use in hepatocyte free uptake assays |
WO2006065751A2 (en) * | 2004-12-13 | 2006-06-22 | Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services | Cpg oligonucleotide prodrugs, compositions thereof and associated therapeutic methods |
US8084589B2 (en) * | 2007-08-31 | 2011-12-27 | University Of Massachusetts | Phosphoramidite nucleoside analogs |
LT2794627T (lt) | 2011-12-22 | 2019-01-10 | Alios Biopharma, Inc. | Pakeistieji nukleozidai, nukleotidai ir jų analogai |
USRE48171E1 (en) | 2012-03-21 | 2020-08-25 | Janssen Biopharma, Inc. | Substituted nucleosides, nucleotides and analogs thereof |
US9441007B2 (en) | 2012-03-21 | 2016-09-13 | Alios Biopharma, Inc. | Substituted nucleosides, nucleotides and analogs thereof |
WO2014197714A1 (en) * | 2013-06-05 | 2014-12-11 | Am Chemicals Llc | Phosphoramidite building blocks for sugar-conjugated oligonucleotides |
Family Cites Families (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4415732A (en) * | 1981-03-27 | 1983-11-15 | University Patents, Inc. | Phosphoramidite compounds and processes |
US4668777A (en) * | 1981-03-27 | 1987-05-26 | University Patents, Inc. | Phosphoramidite nucleoside compounds |
CA1223831A (en) * | 1982-06-23 | 1987-07-07 | Dean Engelhardt | Modified nucleotides, methods of preparing and utilizing and compositions containing the same |
US4547569A (en) * | 1982-11-24 | 1985-10-15 | The United States Of America As Represented By The Department Of Health And Human Services | Intercalating agents specifying nucleotides |
FR2540122B1 (fr) * | 1983-01-27 | 1985-11-29 | Centre Nat Rech Scient | Nouveaux composes comportant une sequence d'oligonucleotide liee a un agent d'intercalation, leur procede de synthese et leur application |
WO1984003285A1 (en) * | 1983-02-22 | 1984-08-30 | Molecular Biosystems Inc | Defined sequence single strand oligonucleotides incorporating reporter groups, process for the chemical synthesis thereof, and nucleosides useful in such synthesis |
JPS59165362A (ja) * | 1983-03-10 | 1984-09-18 | Mitsubishi Electric Corp | 高圧放電灯 |
DE3329892A1 (de) * | 1983-08-18 | 1985-03-07 | Köster, Hubert, Prof. Dr., 2000 Hamburg | Verfahren zur herstellung von oligonucleotiden |
DE3332068A1 (de) * | 1983-09-06 | 1985-03-21 | Hoechst Ag, 6230 Frankfurt | Verfahren zur herstellung von nukleosidalkyl-, aralkyl- und arylphosphoniten und -phosphonaten |
FR2568254B1 (fr) * | 1984-07-25 | 1988-04-29 | Centre Nat Rech Scient | Application d'oligonucleotides lies a un agent intercalant, notamment a titre de medicament |
DE3431536A1 (de) * | 1984-08-28 | 1986-03-13 | Boehringer Mannheim Gmbh, 6800 Mannheim | Derivatisierte nucleinsaeure-sequenz, verfahren zu deren herstellung sowie deren verwendung zum nachweis von nucleinsaeuren |
JPH064670B2 (ja) * | 1984-08-28 | 1994-01-19 | 有機合成薬品工業株式会社 | 酵素標識化されたポリヌクレオチドおよびその製法 |
JPS61112093A (ja) * | 1984-11-05 | 1986-05-30 | Wakunaga Seiyaku Kk | ヌクレオチド誘導体 |
US5252760A (en) * | 1985-03-28 | 1993-10-12 | Chiron Corporation | Method of using colored phosphorylating reagents |
US4762779A (en) * | 1985-06-13 | 1988-08-09 | Amgen Inc. | Compositions and methods for functionalizing nucleic acids |
EP0216357A3 (en) * | 1985-09-25 | 1988-08-31 | Nippon Zeon Co., Ltd. | Phosphoramidite compounds and process for production thereof |
DE3642939A1 (de) * | 1986-06-03 | 1987-12-10 | Europ Lab Molekularbiolog | Verfahren zur dna-markierung |
JPS638396A (ja) * | 1986-06-30 | 1988-01-14 | Wakunaga Pharmaceut Co Ltd | ポリ標識化オリゴヌクレオチド誘導体 |
EP0290583B1 (fr) * | 1986-12-02 | 1993-04-07 | Centre National De La Recherche Scientifique (Cnrs) | OLIGONUCLEOTIDES $g(a) |
AU619170B2 (en) * | 1987-01-09 | 1992-01-23 | Abbott Laboratories | Diagnostic assays using nucleic acid probes |
DD273445A1 (de) * | 1988-06-29 | 1989-11-15 | Akad Wissenschaften Ddr | Verfahren zur herstellung von kovalenten konjugaten aus oligodesoxynukleotiden und polyaminosaeuren oder proteinen |
SE8802574D0 (sv) * | 1988-07-08 | 1988-07-08 | Wallac Oy | Oligonucleotide hybridization probes and means for the synthesis of the most preferred probes |
DE3916871A1 (de) * | 1989-05-24 | 1990-11-29 | Boehringer Mannheim Gmbh | Modifiziertes phosphoramidit-verfahren zur herstellung von modifizierten nukleinsaeuren |
-
1989
- 1989-05-24 DE DE3916871A patent/DE3916871A1/de not_active Withdrawn
-
1990
- 1990-05-15 ES ES90109092T patent/ES2068279T3/es not_active Expired - Lifetime
- 1990-05-15 AT AT90109092T patent/ATE116327T1/de not_active IP Right Cessation
- 1990-05-15 DE DE59008105T patent/DE59008105D1/de not_active Expired - Lifetime
- 1990-05-15 EP EP90109092A patent/EP0399330B1/de not_active Expired - Lifetime
- 1990-05-15 DK DK90109092.8T patent/DK0399330T3/da active
- 1990-05-21 KR KR1019900007260A patent/KR920007274B1/ko not_active IP Right Cessation
- 1990-05-22 JP JP2132429A patent/JPH0730108B2/ja not_active Expired - Lifetime
- 1990-05-23 CA CA002017369A patent/CA2017369C/en not_active Expired - Lifetime
- 1990-05-23 ZA ZA903975A patent/ZA903975B/xx unknown
-
1992
- 1992-07-13 KR KR1019920012473A patent/KR920007558B1/ko not_active IP Right Cessation
-
1994
- 1994-09-19 JP JP6223299A patent/JP2710756B2/ja not_active Expired - Lifetime
-
1995
- 1995-01-10 US US08/370,836 patent/US5700919A/en not_active Expired - Lifetime
- 1995-02-28 GR GR950400427T patent/GR3015223T3/el unknown
-
1997
- 1997-09-19 US US08/934,018 patent/US5902878A/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
KR920007558B1 (ko) | 1992-09-07 |
DE59008105D1 (de) | 1995-02-09 |
JPH0730108B2 (ja) | 1995-04-05 |
DK0399330T3 (da) | 1995-05-15 |
US5700919A (en) | 1997-12-23 |
CA2017369A1 (en) | 1990-11-24 |
JPH07233188A (ja) | 1995-09-05 |
KR920007274B1 (ko) | 1992-08-29 |
EP0399330A1 (de) | 1990-11-28 |
JPH035495A (ja) | 1991-01-11 |
EP0399330B1 (de) | 1994-12-28 |
US5902878A (en) | 1999-05-11 |
JP2710756B2 (ja) | 1998-02-10 |
CA2017369C (en) | 2001-01-23 |
ATE116327T1 (de) | 1995-01-15 |
DE3916871A1 (de) | 1990-11-29 |
ZA903975B (en) | 1991-03-27 |
GR3015223T3 (en) | 1995-05-31 |
KR900018133A (ko) | 1990-12-20 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
ES2068279T3 (es) | Procedimiento modificado del fosforoamidito para la preparacion de acidos nucleicos modificados. | |
NL300131I1 (nl) | Pro-geneesmiddelen van fosfonaten. | |
FI911152A0 (fi) | Amplifikationsfoerfaranden av nukleinsyrasekvens. | |
ATE38386T1 (de) | Verfahren zur herstellung von oligonucleotiden. | |
ES2149157T3 (es) | Nuevo procedimiento para la preparacion de nucleotidos. | |
Seeberger et al. | 2'-Deoxynucleoside Dithiophosphates: Synthesis and Biological Studies1 | |
GB2007670A (en) | Triester process for the synthesis of oligonucleotides | |
ATE57703T1 (de) | Verfahren zur herstellung von 2,6-substituierten 9-(1,3-dihydroxy-2-propoxymethyl)-purinen und einige derivate. | |
ES2061837T3 (es) | Procedimiento para la obtencion continua de acido 2-fosfonobutano-1,2,4-tricarboxilico y sus sales de metales alcalinos. | |
Pon et al. | Necessary protection of the O6-position of guanine during the solid phase synthesis of oligonucleotides by the phosphoramidite approach | |
Connolly | Solid phase 5′-phosphorylation of oligonucleotides | |
PT87916A (pt) | Processo para a preparacao de fosfono-alquil-fosfinatos de aminometileno | |
Theisen et al. | N-6-Dialkylformamidine-2′-deoxyadenosine Phosphoramidites in Oligodeoxynucleotide Synthesis Raped Deprotection of Oligodeoxynucleotides | |
Szemzo et al. | First synthesis of carbocyclic oligothymidylates | |
Seela et al. | Phosphoramidites of [180] Chiral (Rp)-and (Sp)-Configurated Dimer-Blocks and their Use in Automated Oligonucleotide Synthesis | |
Takaku et al. | Use of the 2-(2-pyridyl) ethyl protecting group in the synthesis of DNA fragments via phosphoramidite intermediates | |
Dowden et al. | Chemical synthesis of the novel Ca2+ messenger NAADP | |
Hosaka et al. | Nucleoside Phosphite, O-Bis (1, 1, 1, 3, 3, 3-hexafluoro-2-propyl) Deoxyribonucleosid-3'-yl Phosphites. A Versatile Synthetic Intermediate for Phosphonate Modified Nucleotide and Oligonucleotide Synthesis. | |
JPS6426594A (en) | Nucleotides and antitumor agent | |
JPS6363694A (ja) | ヌクレオシドホスホロチオイットおよびその合成法 | |
MA20825A1 (fr) | Procede de synthese de tetraalkyl-ethenylidenebis phosphonates |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
Ref document number: 399330 Country of ref document: ES |