ES2046999T3 - Un metodo para la purificacion de interferon. - Google Patents
Un metodo para la purificacion de interferon.Info
- Publication number
- ES2046999T3 ES2046999T3 ES88111201T ES88111201T ES2046999T3 ES 2046999 T3 ES2046999 T3 ES 2046999T3 ES 88111201 T ES88111201 T ES 88111201T ES 88111201 T ES88111201 T ES 88111201T ES 2046999 T3 ES2046999 T3 ES 2046999T3
- Authority
- ES
- Spain
- Prior art keywords
- interference
- aqueous solution
- gross
- column
- pyrogenic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title abstract 3
- 238000000746 purification Methods 0.000 title abstract 2
- 102000014150 Interferons Human genes 0.000 title 1
- 108010050904 Interferons Proteins 0.000 title 1
- 229940079322 interferon Drugs 0.000 title 1
- 239000007864 aqueous solution Substances 0.000 abstract 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 3
- 239000008367 deionised water Substances 0.000 abstract 2
- 229910021641 deionized water Inorganic materials 0.000 abstract 2
- 239000000243 solution Substances 0.000 abstract 2
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 229920001296 polysiloxane Polymers 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000011084 recovery Methods 0.000 abstract 1
- 239000002904 solvent Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/565—IFN-beta
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Gastroenterology & Hepatology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
UN METODO ES DESCRITO PARA LA PURIFICACION DE INTERFERENCIAS HUMANAS BRUTAS DE SOLUCIONES QUE LO CONTENGAN, QUE COMPRENDE: A) LA COMPLETA ABSORCION DE LA INTERFERENCIA BRUTA EN UNA COLUMNA DE MATERIAL SILICEO QUE PREVIAMENTE HA SIDO DESINFECTADA CON UNA SOLUCION ACUOSA DE FORMA DE NIDO; B) EL LAVADO DE LA COMNA NO PIROGENICA, ESTERIL, AGUA DESIONIZADA; C) EL TRASLADO DE LAS PROTEINAS RESIDUALES EXTRAÑAS POR ELEVACION DE LA COLUMNA SUCESIVAMENTE CON UNA SOLUCION ACUOSA DE 1.4.M DE CINA, CON AGUA DESIONIZADA, ESTERIL, NO PIROGENICA, YU CON UNA SOLUCION ACUOSA DE UN SOLVENTE QUE TENGA PROPIEDADES HIDROFOBICA Y ACIDA EN UNA CONCENTRACION MOLAR DE 0,001 M A 0,003M; D) LA ELUCION DE UNA INTERFERENCIA DESDE LA COLUMNA CON UNA SOLUCION ACUOSA DEL MISMO SOLVENTE COMO ES USADO EN EL PASO C) A UNA CONCENTRACION MOLAR DE 0,01M A 0,33M Y FINALMENTE; E)LA RECUPERACION Y TIOFILIZACION DE LA FRACCION ELUCIDA Y/O FRACCIONES QUE CONTENGAN LA INTERFERENCIA PURIFICADA.; EL METODO PERMITE A LA INTERFERENCIASER OBTENIDA, LA PUREZA DE LA CUAL, MEDIDA COMO SU ACTIVIDAD ESPECIFICA, ES AL MENOS 1.000 VECES MAS GRANDE QUE ESA DE LA INTERFERENCIA BRUTA, CON UNA PRODUCCION MAS GRANDE QUE EL 85%. EL LA INTERFERENCIA ASI PURIFICADA ES PARTICULARMENTE UTIL PARA TRATAMIENTO EN HOMBRES.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT21560/87A IT1222427B (it) | 1987-07-31 | 1987-07-31 | Procedimento per la purificazione di interferone |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2046999T3 true ES2046999T3 (es) | 1994-02-16 |
Family
ID=11183608
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES88111201T Expired - Lifetime ES2046999T3 (es) | 1987-07-31 | 1988-07-13 | Un metodo para la purificacion de interferon. |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US5066786A (es) |
| EP (1) | EP0301314B1 (es) |
| AT (1) | ATE98257T1 (es) |
| DE (1) | DE3886118T2 (es) |
| ES (1) | ES2046999T3 (es) |
| IT (1) | IT1222427B (es) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1240612B (it) * | 1990-03-16 | 1993-12-17 | Sclavo Spa | Procedimento di purificazione del b-interferone umano ricombinante |
| EP0551535A1 (en) * | 1992-01-13 | 1993-07-21 | SCLAVO S.p.A. | Process for the purification of recombinant human beta interferon, beta interferon thus purified, and pharmaceutical compositions which contain them |
| EP0626448A3 (de) * | 1993-05-26 | 1998-01-14 | BOEHRINGER INGELHEIM INTERNATIONAL GmbH | Verfahren zur Herstellung und Reinigung von alpha-Interferon |
| IL155788A0 (en) * | 2000-11-07 | 2003-12-23 | Chiron Corp | Stabilized interferon compositions |
| KR100524871B1 (ko) * | 2003-12-04 | 2005-10-31 | 씨제이 주식회사 | 인터페론 베타의 정제방법 |
| KR100524872B1 (ko) * | 2003-12-04 | 2005-11-01 | 씨제이 주식회사 | 인터페론 베타의 정제방법 |
| CA2553040A1 (en) | 2004-02-02 | 2005-08-18 | Ambrx, Inc. | Modified human four helical bundle polypeptides and their uses |
| RU2473696C1 (ru) * | 2011-07-14 | 2013-01-27 | Закрытое акционерное общество "ГЕНЕРИУМ" | ПРОМЫШЛЕННЫЙ СПОСОБ ПОЛУЧЕНИЯ И ОЧИСТКИ РЕКОМБИНАНТНОГО ИНТЕРФЕРОНА β-1b ЧЕЛОВЕКА ИЗ ТЕЛЕЦ ВКЛЮЧЕНИЯ |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3897309A (en) * | 1974-02-15 | 1975-07-29 | Merck & Co Inc | Process for removing pyrogenic material from aqueous solutions |
| NL7605805A (nl) * | 1976-05-28 | 1977-11-30 | Stichting Rega V Z W | Werkwijze voor het zuiveren van interferon. |
| NL7907791A (nl) * | 1979-10-23 | 1981-04-27 | Stichting Rega V Z W | Werkwijze voor het zuiveren van interferon. |
| US4485038A (en) * | 1982-10-19 | 1984-11-27 | Health Research (Roswell Park Division) | Method for the production and purification of human leukocyte interferon |
| US4485017A (en) * | 1982-12-22 | 1984-11-27 | Cetus Corporation | Isolation of human interferon by immunosorbent and high performance liquid chromatography |
| IL67896A (en) * | 1983-02-13 | 1987-03-31 | Yeda Res & Dev | Two biologically active human gama interferon subtypes,purification thereof and pharmaceutical compositions containing them |
| US4894330A (en) * | 1986-12-23 | 1990-01-16 | Cetus Corporation | Purification of recombinant beta-interferon incorporating RP-HPLC |
-
1987
- 1987-07-31 IT IT21560/87A patent/IT1222427B/it active
-
1988
- 1988-07-13 DE DE88111201T patent/DE3886118T2/de not_active Expired - Fee Related
- 1988-07-13 ES ES88111201T patent/ES2046999T3/es not_active Expired - Lifetime
- 1988-07-13 EP EP88111201A patent/EP0301314B1/en not_active Expired - Lifetime
- 1988-07-13 AT AT88111201T patent/ATE98257T1/de not_active IP Right Cessation
- 1988-07-18 US US07/220,312 patent/US5066786A/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP0301314A2 (en) | 1989-02-01 |
| EP0301314B1 (en) | 1993-12-08 |
| US5066786A (en) | 1991-11-19 |
| IT1222427B (it) | 1990-09-05 |
| IT8721560A0 (it) | 1987-07-31 |
| ATE98257T1 (de) | 1993-12-15 |
| DE3886118D1 (de) | 1994-01-20 |
| DE3886118T2 (de) | 1994-04-07 |
| EP0301314A3 (en) | 1990-05-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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