ES2034034T3 - Metodo para conservar capas trasplantables de epitelio cultivado in vitro. - Google Patents

Metodo para conservar capas trasplantables de epitelio cultivado in vitro.

Info

Publication number
ES2034034T3
ES2034034T3 ES198888109528T ES88109528T ES2034034T3 ES 2034034 T3 ES2034034 T3 ES 2034034T3 ES 198888109528 T ES198888109528 T ES 198888109528T ES 88109528 T ES88109528 T ES 88109528T ES 2034034 T3 ES2034034 T3 ES 2034034T3
Authority
ES
Spain
Prior art keywords
layers
preserving
transplantable
epithelium
cultivated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198888109528T
Other languages
English (en)
Inventor
Ranieri Cancedda
Michele De Luca
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Istituto Nazionale per la Ricerca Sul Cancro
Original Assignee
Istituto Nazionale per la Ricerca Sul Cancro
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Istituto Nazionale per la Ricerca Sul Cancro filed Critical Istituto Nazionale per la Ricerca Sul Cancro
Application granted granted Critical
Publication of ES2034034T3 publication Critical patent/ES2034034T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0278—Physical preservation processes
    • A01N1/0284—Temperature processes, i.e. using a designated change in temperature over time
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/10—Hair or skin implants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/60—Materials for use in artificial skin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Transplantation (AREA)
  • Zoology (AREA)
  • Veterinary Medicine (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Dermatology (AREA)
  • Wood Science & Technology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Dentistry (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cardiology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Vascular Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

UN PROCEDIMIENTO PARA CONSERVAR CAPAS EPITELIALES CULTIVADAS POR INCUBACION DE LAS MISMAS A TEMPERATURA AMBIENTE EN MEDIOS CON UNA GLICERINA O DIMETILSULFOXIDO COMO CRIOCONSERVANTE POR UN PERIODO DE TIEMPO PREDETERMINADO Y CONGELANDO DESPUES LAS CAPAS CULTIVADAS. EL GRADIENTE DE ENFRIAMIENTO SE CARACTERIZA POR TENER UN VALOR INICIAL BAJO SEGUIDO POR UN VALOR MAYOR DEL ENFRIAMIENTO.
ES198888109528T 1987-06-23 1988-06-15 Metodo para conservar capas trasplantables de epitelio cultivado in vitro. Expired - Lifetime ES2034034T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT8721005A IT1207525B (it) 1987-06-23 1987-06-23 Metodo per la preservazione difogli trapiantabili di epitelio coltivato in vitro vitale.

Publications (1)

Publication Number Publication Date
ES2034034T3 true ES2034034T3 (es) 1993-04-01

Family

ID=11175297

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198888109528T Expired - Lifetime ES2034034T3 (es) 1987-06-23 1988-06-15 Metodo para conservar capas trasplantables de epitelio cultivado in vitro.

Country Status (25)

Country Link
US (1) US5298417A (es)
EP (1) EP0296475B1 (es)
JP (1) JP2644024B2 (es)
KR (1) KR960009482B1 (es)
AR (1) AR240061A1 (es)
AT (1) ATE79720T1 (es)
AU (1) AU618012B2 (es)
BR (1) BR8807104A (es)
CA (1) CA1330541C (es)
DE (1) DE3874019T2 (es)
DK (1) DK81789A (es)
ES (1) ES2034034T3 (es)
FI (1) FI94147C (es)
GR (1) GR3006069T3 (es)
HU (1) HU203827B (es)
IE (1) IE61449B1 (es)
IL (1) IL86806A (es)
IN (1) IN167396B (es)
IT (1) IT1207525B (es)
MX (1) MX11997A (es)
NO (1) NO177211C (es)
OA (1) OA09038A (es)
PT (1) PT87789B (es)
WO (1) WO1988010068A1 (es)
ZA (1) ZA884455B (es)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5482834A (en) * 1982-05-17 1996-01-09 Hahnemann University Evaluation of nucleic acids in a biological sample hybridization in a solution of chaotrophic salt solubilized cells
US5171660A (en) * 1989-04-26 1992-12-15 Cryolife, Inc. Process of revitalizing cells and tissue prior to cryopreservation
DE3941873A1 (de) * 1989-12-19 1991-06-20 Jakob Dr Bodziony Hohlfaser mit der beschichtung von zellen, die mehrjaehrige implantation in arterien und venen ermoeglichen
US5100676A (en) * 1990-02-02 1992-03-31 Biosurface Technology, Inc. Cool storage of cultured epithelial sheets
DE4012079C2 (de) * 1990-04-14 1997-11-06 Jakob Dr Bodziony Implantierbare Austausch- und Diffusionskammer
US5145770A (en) * 1990-06-04 1992-09-08 Biosurface Technology, Inc. Cryopreservation of cultured epithelial sheets
US5192312A (en) * 1991-03-05 1993-03-09 Colorado State University Research Foundation Treated tissue for implantation and methods of treatment and use
US6585969B1 (en) 1991-11-20 2003-07-01 N. V. Innogenetics S.A. Non-viable keratinocyte cell composition or lysate for promoting wound healing
EP0970701B1 (en) * 1991-11-20 2005-10-26 Innogenetics N.V. Pellets derived from keratinocytes for use as wound healing substances
JPH0646840A (ja) * 1992-08-04 1994-02-22 Nippon Zenyaku Kogyo Kk 細胞凍結保存液
IT1263023B (it) * 1992-11-09 1996-07-23 Biostruttura epitelio-supporto tubulare e metodo per la sua preparazione.
IT1256621B (it) * 1992-12-04 1995-12-12 Soluzioni crioprotettrici
US5518878A (en) * 1993-09-15 1996-05-21 Organogenesis Inc. Cryopreservation of cultured skin or cornea equivalents with agitation
US5891617A (en) * 1993-09-15 1999-04-06 Organogenesis Inc. Cryopreservation of harvested skin and cultured skin or cornea equivalents by slow freezing
DE69532976T2 (de) * 1994-03-14 2005-05-04 Cryolife, Inc. Herstellungsverfahren von gewebe zur implantation
JPH10509610A (ja) * 1994-11-09 1998-09-22 クリ・ハークチ，ワリド 創傷修復用包帯およびそれらの保存法
US5689961A (en) 1996-01-30 1997-11-25 Organogenesis Inc. Ice seeding apparatus for cryopreservation systems
US6713084B1 (en) * 1997-01-17 2004-03-30 Celadon Science, Llc Methods for promoting healing of skin resurfacing wounds
AU6242298A (en) 1997-01-17 1998-08-07 Celadon Science, Llc Methods for promoting healing of corneal resurfacing wounds
EP1269840A4 (en) * 2000-03-24 2009-05-06 Menicon Co Ltd METHOD FOR PRESERVING A TISSUE TREATMENT AND IN THE FROZEN CONSERVATED TISSUE REPLACEMENT
IL157532A0 (en) * 2000-12-27 2004-03-28 Ortec International Inc Processes for making cryopreserved composite living constructs and products resulting therefrom
DE10151296A1 (de) * 2001-10-17 2003-04-30 Boehringer Ingelheim Pharma Keratinozyten verwendbar als biologisch aktive Substanz bei der Behandlung von Wunden
WO2003064634A1 (fr) * 2002-01-31 2003-08-07 Asahi Techno Glass Corporation Liquide permettant le stockage a l'etat congele de cellules souches embryonnaires de primates et procede de stockage par congelation
AU2003286474A1 (en) * 2002-10-18 2004-05-04 The General Hospital Corporation Compositions, solutions, and methods used for transplantation
US20070185238A1 (en) * 2006-02-06 2007-08-09 No-Burn Investments, Llc Paint with mold inhibitor and insecticide
CA2644091C (en) * 2008-10-20 2015-12-15 University Of Guelph Embryo culture media containing thyroid hormone
JP2015518843A (ja) 2012-05-25 2015-07-06 ベーリンガー インゲルハイム インターナショナル ゲゼルシャフト ミット ベシュレンクテル ハフツング 創傷、例えば、糖尿病性創傷の処置における、ｄｐｐ−４阻害剤と組み合わせてもよい生物活性物質としてのケラチン生成細胞の使用
EP3207122B1 (en) 2014-10-14 2019-05-08 FUJIFILM Cellular Dynamics, Inc. Generation of keratinocytes from pluripotent stem cells and maintenance of keratinocyte cultures

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1395002A (fr) * 1963-03-01 1965-04-09 Union Carbide Corp Procédé de conservation de cellules
US3943993A (en) * 1974-05-03 1976-03-16 Smith Kendall O Low temperature storage of surface attached living cell cultures
US3940943A (en) * 1975-01-22 1976-03-02 The Curators Of The University Of Missouri Multistage freezing system for preservation of biological materials
US4016036A (en) * 1975-11-14 1977-04-05 Massachusetts Institute Of Technology Process for serially culturing keratinocytes
DE2715887A1 (de) * 1977-04-09 1978-10-12 Ayguen Suerayya Tahsin Prof Dr Verfahren zur herstellung und konservierung von human-zellkulturen aus menschlichen foeten sowie nach diesem verfahren hergestellte human-zellkulturen fuer die intravenoese oder intramuskulaere anwendung beim menschlichen koerper
US4456687A (en) * 1978-11-16 1984-06-26 President And Fellows Of Harvard College Agents for promoting growth of epithelial cells
JPS55501130A (es) * 1978-12-26 1980-12-18
JPS6029471B2 (ja) * 1979-08-16 1985-07-10 日機装株式会社 肝細胞の凍結方法
US4304866A (en) * 1979-11-14 1981-12-08 Massachusetts Institute Of Technology Transplantable sheets of living keratinous tissue
FR2493662A1 (fr) * 1980-11-05 1982-05-07 Rhone Poulenc Ind Substrats metallises pour circuits imprimes et leur procede de preparation
US4673649A (en) * 1983-07-15 1987-06-16 University Patents, Inc. Process and defined medium for growth of human epidermal keratinocyte cells
CA2000181A1 (en) * 1988-10-14 1990-04-14 Chung-Faye Chao Process for producing cultured epidermal sheet, product and method of use

Also Published As

Publication number Publication date
IN167396B (es) 1990-10-20
MX11997A (es) 1993-06-01
DE3874019D1 (de) 1992-10-01
FI94147B (fi) 1995-04-13
DK81789D0 (da) 1989-02-22
DE3874019T2 (de) 1993-01-21
AU1995788A (en) 1989-01-19
PT87789B (pt) 1994-11-30
AR240061A1 (es) 1990-01-31
IT8721005A0 (it) 1987-06-23
GR3006069T3 (es) 1993-06-21
HUT51848A (en) 1990-06-28
OA09038A (es) 1991-03-31
FI94147C (fi) 1995-07-25
KR960009482B1 (en) 1996-07-20
IE881889L (en) 1988-12-23
JP2644024B2 (ja) 1997-08-25
HU203827B (en) 1991-10-28
NO177211B (no) 1995-05-02
ZA884455B (en) 1989-03-29
NO177211C (no) 1995-08-09
NO890715L (no) 1989-02-20
ATE79720T1 (de) 1992-09-15
AU618012B2 (en) 1991-12-12
FI890821A0 (fi) 1989-02-21
IE61449B1 (en) 1994-11-02
JPH02500800A (ja) 1990-03-22
EP0296475B1 (en) 1992-08-26
FI890821A (fi) 1989-02-21
CA1330541C (en) 1994-07-05
IL86806A (en) 1992-02-16
BR8807104A (pt) 1989-10-17
IT1207525B (it) 1989-05-25
NO890715D0 (no) 1989-02-20
EP0296475A1 (en) 1988-12-28
US5298417A (en) 1994-03-29
PT87789A (pt) 1989-05-31
DK81789A (da) 1989-02-22
WO1988010068A1 (en) 1988-12-29

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