EP4161473A1 - Use of a compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups as a microbiome regulator on scalp - Google Patents
Use of a compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups as a microbiome regulator on scalpInfo
- Publication number
- EP4161473A1 EP4161473A1 EP20939406.3A EP20939406A EP4161473A1 EP 4161473 A1 EP4161473 A1 EP 4161473A1 EP 20939406 A EP20939406 A EP 20939406A EP 4161473 A1 EP4161473 A1 EP 4161473A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- scalp
- glycol
- compound
- hydroxyl groups
- microbiome regulator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000004761 scalp Anatomy 0.000 title claims abstract description 105
- 150000001875 compounds Chemical class 0.000 title claims abstract description 64
- 244000005700 microbiome Species 0.000 title claims abstract description 56
- 125000002887 hydroxy group Chemical group [H]O* 0.000 title claims abstract description 55
- 230000001476 alcoholic effect Effects 0.000 title claims abstract description 25
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 title claims abstract description 25
- 239000000203 mixture Substances 0.000 claims abstract description 92
- 230000002401 inhibitory effect Effects 0.000 claims abstract description 64
- 241000186427 Cutibacterium acnes Species 0.000 claims abstract description 56
- 241000191963 Staphylococcus epidermidis Species 0.000 claims abstract description 41
- 210000002966 serum Anatomy 0.000 claims abstract description 32
- 239000002453 shampoo Substances 0.000 claims abstract description 32
- 238000000034 method Methods 0.000 claims abstract description 20
- 238000012360 testing method Methods 0.000 claims abstract description 20
- 125000004432 carbon atom Chemical group C* 0.000 claims abstract description 19
- 238000012216 screening Methods 0.000 claims abstract description 8
- VGEREEWJJVICBM-UHFFFAOYSA-N phloretin Chemical compound C1=CC(O)=CC=C1CCC(=O)C1=C(O)C=C(O)C=C1O VGEREEWJJVICBM-UHFFFAOYSA-N 0.000 claims description 24
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 18
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 18
- SVTBMSDMJJWYQN-UHFFFAOYSA-N 2-methylpentane-2,4-diol Chemical compound CC(O)CC(C)(C)O SVTBMSDMJJWYQN-UHFFFAOYSA-N 0.000 claims description 16
- YSRSBDQINUMTIF-SNVBAGLBSA-N (2r)-decane-1,2-diol Chemical compound CCCCCCCC[C@@H](O)CO YSRSBDQINUMTIF-SNVBAGLBSA-N 0.000 claims description 12
- ZWTDXYUDJYDHJR-UHFFFAOYSA-N (E)-1-(2,4-dihydroxyphenyl)-3-(2,4-dihydroxyphenyl)-2-propen-1-one Natural products OC1=CC(O)=CC=C1C=CC(=O)C1=CC=C(O)C=C1O ZWTDXYUDJYDHJR-UHFFFAOYSA-N 0.000 claims description 12
- NCZPCONIKBICGS-UHFFFAOYSA-N 3-(2-ethylhexoxy)propane-1,2-diol Chemical compound CCCCC(CC)COCC(O)CO NCZPCONIKBICGS-UHFFFAOYSA-N 0.000 claims description 12
- YQHMWTPYORBCMF-UHFFFAOYSA-N Naringenin chalcone Natural products C1=CC(O)=CC=C1C=CC(=O)C1=C(O)C=C(O)C=C1O YQHMWTPYORBCMF-UHFFFAOYSA-N 0.000 claims description 12
- 229940097037 decylene glycol Drugs 0.000 claims description 12
- 229940100524 ethylhexylglycerin Drugs 0.000 claims description 12
- 229940043375 1,5-pentanediol Drugs 0.000 claims description 10
- AEIJTFQOBWATKX-UHFFFAOYSA-N octane-1,2-diol Chemical compound CCCCCCC(O)CO AEIJTFQOBWATKX-UHFFFAOYSA-N 0.000 claims description 10
- WCVRQHFDJLLWFE-UHFFFAOYSA-N pentane-1,2-diol Chemical compound CCCC(O)CO WCVRQHFDJLLWFE-UHFFFAOYSA-N 0.000 claims description 10
- 235000011187 glycerol Nutrition 0.000 claims description 9
- 229960005150 glycerol Drugs 0.000 claims description 8
- 229940051250 hexylene glycol Drugs 0.000 claims description 8
- 239000002562 thickening agent Substances 0.000 claims description 8
- 239000003795 chemical substances by application Substances 0.000 claims description 6
- 230000003750 conditioning effect Effects 0.000 claims description 6
- 229960004063 propylene glycol Drugs 0.000 claims description 6
- 239000002904 solvent Substances 0.000 claims description 6
- 239000004094 surface-active agent Substances 0.000 claims description 5
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 claims description 4
- SZXQTJUDPRGNJN-UHFFFAOYSA-N dipropylene glycol Chemical compound OCCCOCCCO SZXQTJUDPRGNJN-UHFFFAOYSA-N 0.000 claims description 4
- 229940113120 dipropylene glycol Drugs 0.000 claims description 4
- 229940015975 1,2-hexanediol Drugs 0.000 claims description 2
- FHKSXSQHXQEMOK-UHFFFAOYSA-N hexane-1,2-diol Chemical compound CCCCC(O)CO FHKSXSQHXQEMOK-UHFFFAOYSA-N 0.000 claims description 2
- 239000000243 solution Substances 0.000 description 17
- 230000001580 bacterial effect Effects 0.000 description 11
- 239000007788 liquid Substances 0.000 description 10
- 239000000523 sample Substances 0.000 description 10
- 239000000126 substance Substances 0.000 description 10
- 208000024891 symptom Diseases 0.000 description 10
- 230000005722 itchiness Effects 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- 239000007787 solid Substances 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 239000012488 sample solution Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 208000001840 Dandruff Diseases 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- -1 Alkyl Glucosides Chemical class 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- NTYJJOPFIAHURM-UHFFFAOYSA-N Histamine Chemical compound NCCC1=CN=CN1 NTYJJOPFIAHURM-UHFFFAOYSA-N 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- SAVLIIGUQOSOEP-UHFFFAOYSA-N N-octanoylglycine Chemical compound CCCCCCCC(=O)NCC(O)=O SAVLIIGUQOSOEP-UHFFFAOYSA-N 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 208000003251 Pruritus Diseases 0.000 description 2
- 241000191940 Staphylococcus Species 0.000 description 2
- 150000001252 acrylic acid derivatives Chemical class 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 229920006037 cross link polymer Polymers 0.000 description 2
- 238000005138 cryopreservation Methods 0.000 description 2
- 210000002615 epidermis Anatomy 0.000 description 2
- 238000009472 formulation Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- TUBPSFQENHCYBW-HVDRVSQOSA-N (2s)-2-aminopentanedioic acid;2-[bis(2-hydroxyethyl)amino]ethanol Chemical compound OC(=O)[C@@H](N)CCC(O)=O.OCCN(CCO)CCO TUBPSFQENHCYBW-HVDRVSQOSA-N 0.000 description 1
- BTGGRPUPMPLZNT-PGEUSFDPSA-N 2,2-bis[[(z)-octadec-9-enoyl]oxymethyl]butyl (z)-octadec-9-enoate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC(CC)(COC(=O)CCCCCCC\C=C/CCCCCCCC)COC(=O)CCCCCCC\C=C/CCCCCCCC BTGGRPUPMPLZNT-PGEUSFDPSA-N 0.000 description 1
- OUNZARDETXBPIX-UHFFFAOYSA-N 2-(2-dodecoxyethoxy)acetic acid Chemical compound CCCCCCCCCCCCOCCOCC(O)=O OUNZARDETXBPIX-UHFFFAOYSA-N 0.000 description 1
- AZLWQVJVINEILY-UHFFFAOYSA-N 2-(2-dodecoxyethoxy)ethanol Chemical compound CCCCCCCCCCCCOCCOCCO AZLWQVJVINEILY-UHFFFAOYSA-N 0.000 description 1
- MQFYRUGXOJAUQK-UHFFFAOYSA-N 2-[2-[2-(2-octadecanoyloxyethoxy)ethoxy]ethoxy]ethyl octadecanoate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCCOCCOCCOCCOC(=O)CCCCCCCCCCCCCCCCC MQFYRUGXOJAUQK-UHFFFAOYSA-N 0.000 description 1
- XVZIAZAFOVOYAT-TTWKNDKESA-N 2-methyloxirane;(e)-octadec-9-enoic acid;oxirane Chemical compound C1CO1.CC1CO1.CCCCCCCC\C=C\CCCCCCCC(O)=O XVZIAZAFOVOYAT-TTWKNDKESA-N 0.000 description 1
- QCDWFXQBSFUVSP-UHFFFAOYSA-N 2-phenoxyethanol Chemical compound OCCOC1=CC=CC=C1 QCDWFXQBSFUVSP-UHFFFAOYSA-N 0.000 description 1
- DQYSALLXMHVJAV-UHFFFAOYSA-M 3-heptyl-2-[(3-heptyl-4-methyl-1,3-thiazol-3-ium-2-yl)methylidene]-4-methyl-1,3-thiazole;iodide Chemical compound [I-].CCCCCCCN1C(C)=CS\C1=C\C1=[N+](CCCCCCC)C(C)=CS1 DQYSALLXMHVJAV-UHFFFAOYSA-M 0.000 description 1
- 208000002874 Acne Vulgaris Diseases 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-M Acrylate Chemical compound [O-]C(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-M 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- 206010013082 Discomfort Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 239000004354 Hydroxyethyl cellulose Substances 0.000 description 1
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 201000004681 Psoriasis Diseases 0.000 description 1
- 240000006079 Schisandra chinensis Species 0.000 description 1
- 235000008422 Schisandra chinensis Nutrition 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 241000751182 Staphylococcus epidermidis ATCC 12228 Species 0.000 description 1
- OCKWAZCWKSMKNC-UHFFFAOYSA-N [3-octadecanoyloxy-2,2-bis(octadecanoyloxymethyl)propyl] octadecanoate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(COC(=O)CCCCCCCCCCCCCCCCC)(COC(=O)CCCCCCCCCCCCCCCCC)COC(=O)CCCCCCCCCCCCCCCCC OCKWAZCWKSMKNC-UHFFFAOYSA-N 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 206010000496 acne Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 125000005250 alkyl acrylate group Chemical group 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- BTBJBAZGXNKLQC-UHFFFAOYSA-N ammonium lauryl sulfate Chemical compound [NH4+].CCCCCCCCCCCCOS([O-])(=O)=O BTBJBAZGXNKLQC-UHFFFAOYSA-N 0.000 description 1
- 229940063953 ammonium lauryl sulfate Drugs 0.000 description 1
- 239000002280 amphoteric surfactant Substances 0.000 description 1
- 239000003945 anionic surfactant Substances 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 229940051368 capryloyl glycine Drugs 0.000 description 1
- 229960001631 carbomer Drugs 0.000 description 1
- 150000001735 carboxylic acids Chemical class 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- MRUAUOIMASANKQ-UHFFFAOYSA-N cocamidopropyl betaine Chemical compound CCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC([O-])=O MRUAUOIMASANKQ-UHFFFAOYSA-N 0.000 description 1
- 229940073507 cocamidopropyl betaine Drugs 0.000 description 1
- 229940080421 coco glucoside Drugs 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- OTKJDMGTUTTYMP-UHFFFAOYSA-N dihydrosphingosine Natural products CCCCCCCCCCCCCCCC(O)C(N)CO OTKJDMGTUTTYMP-UHFFFAOYSA-N 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- SYELZBGXAIXKHU-UHFFFAOYSA-N dodecyldimethylamine N-oxide Chemical compound CCCCCCCCCCCC[N+](C)(C)[O-] SYELZBGXAIXKHU-UHFFFAOYSA-N 0.000 description 1
- 230000002222 downregulating effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 235000020993 ground meat Nutrition 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000008236 heating water Substances 0.000 description 1
- 229960001340 histamine Drugs 0.000 description 1
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229940048866 lauramine oxide Drugs 0.000 description 1
- 229940100491 laureth-2 Drugs 0.000 description 1
- PYIDGJJWBIBVIA-UYTYNIKBSA-N lauryl glucoside Chemical compound CCCCCCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O PYIDGJJWBIBVIA-UYTYNIKBSA-N 0.000 description 1
- 229940048848 lauryl glucoside Drugs 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000008176 lyophilized powder Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229940044591 methyl glucose dioleate Drugs 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000005142 microbroth dilution method Methods 0.000 description 1
- 239000012982 microporous membrane Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 229940086560 pentaerythrityl tetrastearate Drugs 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 229960005323 phenoxyethanol Drugs 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 229940055019 propionibacterium acne Drugs 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 description 1
- 108700004121 sarkosyl Proteins 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 1
- 235000010234 sodium benzoate Nutrition 0.000 description 1
- 239000004299 sodium benzoate Substances 0.000 description 1
- 229940031688 sodium c14-16 olefin sulfonate Drugs 0.000 description 1
- 229940079776 sodium cocoyl isethionate Drugs 0.000 description 1
- 229940057950 sodium laureth sulfate Drugs 0.000 description 1
- 229940102544 sodium laureth-13 carboxylate Drugs 0.000 description 1
- KSAVQLQVUXSOCR-UHFFFAOYSA-M sodium lauroyl sarcosinate Chemical compound [Na+].CCCCCCCCCCCC(=O)N(C)CC([O-])=O KSAVQLQVUXSOCR-UHFFFAOYSA-M 0.000 description 1
- 229940045885 sodium lauroyl sarcosinate Drugs 0.000 description 1
- 229940048109 sodium methyl cocoyl taurate Drugs 0.000 description 1
- SXHLENDCVBIJFO-UHFFFAOYSA-M sodium;2-[2-(2-dodecoxyethoxy)ethoxy]ethyl sulfate Chemical compound [Na+].CCCCCCCCCCCCOCCOCCOCCOS([O-])(=O)=O SXHLENDCVBIJFO-UHFFFAOYSA-M 0.000 description 1
- HVFAVOFILADWEZ-UHFFFAOYSA-M sodium;2-[2-(dodecanoylamino)ethyl-(2-hydroxyethyl)amino]acetate Chemical compound [Na+].CCCCCCCCCCCC(=O)NCCN(CCO)CC([O-])=O HVFAVOFILADWEZ-UHFFFAOYSA-M 0.000 description 1
- 241000894007 species Species 0.000 description 1
- OTKJDMGTUTTYMP-ZWKOTPCHSA-N sphinganine Chemical compound CCCCCCCCCCCCCCC[C@@H](O)[C@@H](N)CO OTKJDMGTUTTYMP-ZWKOTPCHSA-N 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 150000003458 sulfonic acid derivatives Chemical class 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 229940048912 triethanolamine cocoyl glutamate Drugs 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000000230 xanthan gum Substances 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
- 235000010493 xanthan gum Nutrition 0.000 description 1
- 229940082509 xanthan gum Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/006—Antidandruff preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/35—Ketones, e.g. benzophenone
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/44—Staphylococcus
- C12R2001/45—Staphylococcus epidermidis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/10—Screening for compounds of potential therapeutic value involving cells
Definitions
- the present invention relates to a use of a compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule, as a microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp; a method of screening a compound or mixture for a microbiome regulator on scalp; and a shampoo or scalp serum composition comprising the microbiome regulator.
- scalp itchiness is the most common scalp discomfort among people. In particular, it is the top scalp concern of Chinese consumers.
- the itchiness is associated with scalp diseases, dandruff, psoriasis, etc., and many of non-dandruff consumers also suffered scalp itchiness.
- the traditional ways to relieve scalp itchiness is to inhibit histamine release, or numb sensorial channels, or reduce redness with actives.
- the traditional solutions are partial solutions to cure single symptoms and can merely cure symptoms in a short period.
- they cannot satisfy the needs of the consumers, who are longing for novel solutions not only curing symptoms in a short period, but also remedying scalp to improve scalp overall condition in a long period.
- the inventors conducted a lot of experiments and an intensive study, and surprisingly obtained a compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule, which can be used as a microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp.
- the present invention provides a use of a compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule, as a microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp.
- the present invention also provides a method of screening a compound or mixture for a microbiome regulator on scalp, comprising:
- the present invention provides a shampoo or scalp serum composition, comprising a microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp, which is a compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule, or the mixture thereof.
- the use and the shampoo or scalp serum composition according to the present invention not only cure symptoms in a short period but also remedy scalp to improve scalp overall condition in a long period. Moreover, by the method according to the present invention, the use and the shampoo or scalp serum which not only cure symptoms in a short period but also remedy scalp to improve scalp overall condition in a long period can be obtained.
- microbiome regulator and “microbiome balancer” are interchangeable, and they both refer to a substance which can regulate the ecological communities of commensal bacteria on the scalp, up-regulating good bacteria and down-regulating bad ones, and then in consequence improve the scalp condition holistically, with moisturized barrier, soothed redness, and relieved scalp itch.
- Minimum Inhibitory Concentration can be abbreviated to “MIC” ; and it refers to the minimum concentration of a chemical that will inhibit the growth of a microorganism, and is usually reported as ⁇ g/ml or mg/ml for a solid compound, and as percentage value for a liquid compound.
- MIC Minimum Inhibitory Concentration
- the “Minimum Inhibitory Concentration” in the present invention is measured according to the method as described in the Examples of the present description.
- Minimum Inhibitory Concentration (Cutibacterium acnes) of the compound or mixture” and “Minimum Inhibitory Concentrations of the compound or mixture towards Cutibacterium acnes” are interchangeable, and they both refer to the minimum concentration of the compound or mixture that will inhibit the growth of Cutibacterium acnes.
- Minimum Inhibitory Concentration (Staphylococcus epidermidis) of the compound or mixture” and “Minimum Inhibitory Concentrations of the compound or mixture towards Staphylococcus epidermidis” are interchangeable, and they both refer to the minimum concentration of the compound or mixture that will inhibit the growth of Staphylococcus epidermidis.
- a compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule can be used as a microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp.
- the compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule has a ratio of Minimum Inhibitory Concentration (Cutibacterium acnes) to Minimum Inhibitory Concentration (Staphylococcus epidermidis) ⁇ 1.
- the ratio of Minimum Inhibitory Concentration (Cutibacterium acnes) to Minimum Inhibitory Concentration (Staphylococcus epidermidis) is not more than 0.9, not more than 0.8, not more than 0.7, not more than 0.6, not more than 0.5, not more than 0.4, not more than 0.3, not more than 0.25, or not more than 0.20.
- the ratio of Minimum Inhibitory Concentration (Cutibacterium acnes) to Minimum Inhibitory Concentration (Staphylococcus epidermidis) is at least 0.01, at least 0.02, or at least 0.025.
- the ratio of Minimum Inhibitory Concentration (Cutibacterium acnes) to Minimum Inhibitory Concentration (Staphylococcus epidermidis) is in a range of 0.01 to 1, preferably 0.02 to 0.9, more preferably 0.025 to 0.5.
- Minimum Inhibitory Concentration Cutibacterium acnes
- Minimum Inhibitory Concentration Staphylococcus epidermidis
- the ratio of Minimum Inhibitory Concentration (Cutibacterium acnes) to Minimum Inhibitory Concentration (Staphylococcus epidermidis) is less than 0.01, it will lead to an imbalance of the bacterium.
- the compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule contains two, three, four or five alcoholic hydroxyl groups or phenolic hydroxyl groups per molecule.
- the compound contains two, three or four alcoholic hydroxyl groups or phenolic hydroxyl groups per molecule. More preferably, the compound contains two alcoholic hydroxyl groups or four phenolic hydroxyl groups per molecule.
- the compound is selected from the group consisting of propylene glycol, butylene glycol, pentylene glycol, hexylene glycol, caprylyl glycol, decylene glycol, dipropylene glycol, glycerin, ethylhexylglycerin and phloretin.
- the compound is selected from the group consisting of pentylene glycol, hexylene glycol, caprylyl glycol, decylene glycol, ethylhexylglycerin and phloretin.
- Examples of commercially available compound according to the present invention include, for example, SymClariol, which is decylene glycol available from Symrise Company; Caprylyl Glycol, which is available from Symrise Company; Pentylene Glycol, which is available from Symrise Company; EthylHexylGlycerin, which is available from Schülke & Mayr GmbH; and Phloretin, which is Phloretin available from Symrise Company.
- a method of screening a compound or mixture for a microbiome regulator on scalp comprises:
- a shampoo or scalp serum composition comprises a microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp, which is a compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule, or the mixture thereof.
- the mircobiome regulator is the compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule as defined above, or the mixture thereof.
- the amount of the microbiome regulator is at least 0.01 wt. %, at least 0.05%, at least 0.1 wt. %, at least 0.5%, or at least 1.0%.
- the amount of the microbiome regulator is not more than 15.0 wt. %, not more than 14 wt. %, not more than 13 wt. %, not more than 12 wt. %, not more than 11 wt. %, or not more than 10 wt. %.
- the amount of the microbiome regulator is within a range of 0.01 wt.
- %to 15.0 wt. % preferably 0.5 wt. %to 13 wt. %, more preferably 1 wt. %to 10 wt. %, based on the total weight of the shampoo or scalp serum composition. If the amount of the microbiome regulator is less than 0.01 wt. %based on the total weight of the shampoo or scalp serum composition, the composition cannot effectively cure symptoms in a short period and remedy scalp to improve scalp overall condition in a long period. If the amount of the microbiome regulator is higher than 15.0 wt. %based on the total weight of the shampoo or scalp serum composition, the formulation will be not stable in a long term and the efficacy delivery of the microbiome regulator will be lower.
- the microbiome regulator contains two alcoholic hydroxyl groups or four phenolic hydroxyl groups per molecule.
- the microbiome regulator is selected from the group consisting of propylene glycol, butylene glycol, pentylene glycol, hexylene glycol, caprylyl glycol, decylene glycol, dipropylene glycol, glycerin, ethylhexylglycerin and phloretin.
- the microbiome regulator is selected from the group consisting of pentylene glycol, hexylene glycol, caprylyl glycol, decylene glycol, ethylhexylglycerin, glycerin and phloretin.
- the microbiome regulator contains ethylhexylglycerin, decylene glycol and glycerin. More preferably, the microbiome regulator further contains 1, 2-hexanediol and/or propylene glycol.
- the shampoo or scalp serum composition according to the present invention may be formulated to contain one or more additional components or additives besides those described above.
- the shampoo composition may additionally comprise a solvent, a surfactant, a thickener, a hair conditioning agent and/or PH adjuster; and the scalp serum composition may additionally comprise a solvent, a thickener, a hair conditioning agent and/or PH adjuster.
- the solvent which can be used in the practice of the present invention can be any solvent conventionally used in the shampoo or scalp serum, and it is preferably Aqua.
- the surfactants which can be used in the practice of the present invention include, but are not limited to:
- Anionic surfactants for example, Acylated Amino Acids, such as Sodium Lauroyl Sarcosinate, and TEA-Cocoyl Glutamate; Carboxylic Acids &salts, such as Sodium Laureth-13 Carboxylate; Sulfonic Acid derivatives, such as Sodium Methyl Cocoyl Taurate, Sodium Cocoyl Isethionate, and Sodium C14-16 Olefin Sulfonate; Sulfuric Acid derivatives, such as Sodium Laureth Sulfate, and Ammonium Lauryl Sulfate;
- Acylated Amino Acids such as Sodium Lauroyl Sarcosinate, and TEA-Cocoyl Glutamate
- Carboxylic Acids &salts such as Sodium Laureth-13 Carboxylate
- Sulfonic Acid derivatives such as Sodium Methyl Cocoyl Taurate, Sodium Cocoyl Isethionate, and Sodium C14-16 Olef
- Nonionic surfactants for example, Alkyl Glucosides, such as Coco-Glucoside and Lauryl Glucoside; and Amine oxides, such as Lauramine Oxide;
- Amphoteric surfactants for example, Alkyl amido Alkyl Amines, such as Sodium Lauroamphoacetate; and Alkyl Betaines, such as Cocamidopropyl betaine, Coco-betaine and coco-hydroxysultaine.
- thickener which can be used for shampoo in the practice of the present invention include, but are not limited to PEG/PPG-120/10 Trimethylolpropane Trioleate, PEG-120 Methyl Glucose Dioleate, PEG-150 Distearate, PEG-150 Pentaerythrityl Tetrastearate, PEG-55 Propylene Glycol Oleate, PPG-2 Hydroxyethyl Cocamide, Laureth-2, Cocamide MEA and NaCl.
- the PH adjuster which can be used in the practice of the present invention includes, but are not limited to citric acid, lactic acid, sodium hydroxide, and potassium hydroxide.
- the shampoo or scalp serum composition according to the present invention can be made by any appropriate method.
- the composition can be made by simply mixing all the components together.
- the shampoo composition can be produced by a method comprising: heating water in a vessel; mixing the surfactants into the water; heating the mixture until the surfactants are fully dispersed; adding the thickener, hair conditioning agents, microbiome regulator and/or PH adjuster and/or other optional components with continuous stirring until all the components are fully dissolved; and cooling down the final mixture to room temperature.
- the scalp serum composition can be produced by a method comprising: mixing the thickener, PH adjuster into water with continuous stirring until a homogeneous gel formed without flocs and lumps; adding hair conditioning agents, microbiome regulator and/or other optional components with continuous stirring until all the components are fully dispersed.
- the shampoo or scalp serum composition can be in solid or liquid state.
- the shampoo can be used in the form of viscous liquid.
- the serum can be used in either gel form or liquid form.
- the shampoo composition can be applied gently on wet hair, and then the hair is rinsed thoroughly.
- the shampoo composition may be used as often as desired.
- the scalp serum composition can be applied evenly over scalp, the scalp can be gently massaged for 2-3 minutes until the composition is fully absorbed, and there is no need to rinse or towel-wipe after use.
- the scalp serum composition may be used whenever there are any discomforts on scalp.
- the compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule according to the present invention can be used as a microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp, and it can be used for not only curing symptoms in a short period but also remedying scalp to improve scalp overall condition in a long period
- the compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule can be obtained.
- the shampoo or scalp serum composition according to the present invention can not only cure symptoms in a short period but also remedies scalp to improve scalp overall condition in a long period.
- SymClariol which is decylene glycol, was manufactured by Symrise Company.
- Caprylyl Glycol was manufactured by Symrise Company.
- Pentylene Glycol was manufactured by Symrise Company.
- Sensiva SC 50 which is EthylHexylGlycerin was manufactured by Schülke & Mayr GmbH.
- Phloretin which is Phloretin, was manufactured by Symrise Company.
- LIPACIDE C8G which is Capryloyl Glycine, was manufactured by Seppic Company.
- Sphingony which is Sphinganine, was manufactured by Evonik Company.
- BIOXYNE which is Quaternium-73, was manufactured by Sethic Company.
- Phenoxyethanol was manufactured by BASF.
- Propionibacterium acnes ATCC6919 was purchased from Shanghai Fuxiang Biotechnology Co., Ltd.
- Staphylococcus epidermidis ATCC12228 was purchased from the mall North Natronian Biotechnology Co., Ltd.
- the solid medium was prepared after being cooled to about 60 °C and poured into a clean sterilization plate.
- the amount of liquid added was about 1/4-1/3 of the anaerobic bottle. Fully purged with nitrogen to remove oxygen and sterilized at 115 °C as preparation.
- Cutibacterium acnes (cultured for 3 ⁇ 5 days, anaerobically) with a sterile syringe, transferred it to a culture flask containing 1/3 volume of ground beef in fresh broth, and kept anaerobic at 37 °C. After 3 to 5 days of culture, it could be passaged or used for subsequent experiments.
- the antibacterial activity of the test substance against Cutibacterium acnes was evaluated by the micro-broth dilution method recommended by the American Association of Clinical and Laboratory Standards (see Dej-Adisai S, Meechai I, Puripattanavong J, Kummee S. Antityrosinase and antimicrobial activities from Thai medicinal plants. Arch Pharm Res, 2014, 37 (4) : 473-483; and Guo M, Lu Y, Yang J, Zhao X, Lu Y. Inhibitory effects of Schisandra chinensis extract on acne-related inflammation and UVB-induced photoageing. Pharmaceutical Biology, 2016, 54 (12) : 2987-2994) to determine the minimum inhibitory concentration (MIC) of the test substance against Cutibacterium acnes.
- MIC minimum inhibitory concentration
- the lyophilized powder was taken out from the refrigerator, resuscitated according to the species instructions, and cultured at 105 rpm with a constant temperature of 37 °C. After 1-2 days of culture, transferred a small amount of bacterial liquid to fresh liquid medium. Generally cultured for 3 generations after resuscitation, it could be used for subsequent experiments.
- the solid powder sample to be tested was first dissolved in DMSO (completely dissolved) , and then filtered through a 0.22 ⁇ M microporous membrane. Diluted twice with the corresponding culture solution, and the appropriate concentration was prepared according to the supplier's recommended amount (DMSO is less than 1%) .
- the insoluble sample was directly diluted with a medium to prepare a suspension, and the liquid sample was diluted with a medium by volume concentration.
- the sample solution needed to be prepared right before use. This process needed to be performed aseptically in a clean bench.
- the 96-well plate was sealed with a parafilm, wrapped in a fresh-keeping bag, and cultured at 37 °C, 105 rpm for 24 h, and the OD value at 530 nm was measured by a microplate reader.
- the positive drug is: penicillin.
- Three sets of parallel experiments were conducted. The minimum inhibitory concentration (MIC) was defined as the lowest concentration of the test substance at which bacterial growth was completely inhibited.
- the direct values of Minimum Inhibitory Concentration (Cutibacterium acnes) and Minimum Inhibitory Concentration (Staphylococcus epidermidis) of all the tested samples were obtained. Then, the calculated values of ( (Minimum Inhibitory Concentration (Cutibacterium acnes) /Minimum Inhibitory Concentration (Staphylococcus epidermidis) ) of all the tested samples were obtained. If the calculated value of a sample is less than 1, the sample is suitable as a microbiome regulator on scalp; whereas if the calculated value of a sample is equal to or higher than 1, the sample is not suitable as a microbiome regulator.
- the compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule can be used as a microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp.
- the compounds of Inventive Examples 1-5 can be used as microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp
- the compounds of Comparative Examples 1-6 which contain one hydroxyl group, eighteen carbon atoms, no hydroxyl group, one hydroxyl group, no hydroxyl group and twenty-three carbon atoms, and one hydroxyl group per molecule, respectively
- the compounds of Comparative Examples 1-6 which contain one hydroxyl group, eighteen carbon atoms, no hydroxyl group, one hydroxyl group, no hydroxyl group and twenty-three carbon atoms, and one hydroxyl group per molecule, respectively
- composition of Example 1 10 subjects were shampooed with the composition of Example 1 every the other day, that is three times shampoos in total. For each shampooing, the composition of Example 1 was applied twice, 6g of each application.
- Example 2 which is a leave-on serum, it was applied on blow-dried scalp of another 10 subjects after washing, 4 g each subject. During the course, the composition of Example 2 was also applied three times in total.
- compositions according to the present invention not only cure scalp itchiness &redness to the subject in this short period but also remedy scalp with improved scalp moisture and reduced inflammation securing a healthy scalp condition in a long run.
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Abstract
Description
- The present invention relates to a use of a compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule, as a microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp; a method of screening a compound or mixture for a microbiome regulator on scalp; and a shampoo or scalp serum composition comprising the microbiome regulator.
- Consumers are having increasing awareness and concerns about the scalp problems, like itchiness, redness, dryness, etc. As is known to all, scalp itchiness is the most common scalp discomfort among people. In particular, it is the top scalp concern of Chinese consumers. The itchiness is associated with scalp diseases, dandruff, psoriasis, etc., and many of non-dandruff consumers also suffered scalp itchiness. The traditional ways to relieve scalp itchiness is to inhibit histamine release, or numb sensorial channels, or reduce redness with actives.
- However, the traditional solutions are partial solutions to cure single symptoms and can merely cure symptoms in a short period. Thus, nowadays they cannot satisfy the needs of the consumers, who are longing for novel solutions not only curing symptoms in a short period, but also remedying scalp to improve scalp overall condition in a long period.
- Therefore, there is a need to develop a solution of scalp care which not only cures symptoms in a short period but also remedies scalp to improve scalp overall condition in a long period, and a method for obtaining such a solution.
- Summary of the invention
- It is an object of the present invention to provide a solution of scalp care which not only cures symptoms in a short period but also remedies scalp to improve scalp overall condition in a long period. It is another object of the present invention is to provide a method for obtaining such a solution.
- The inventors found that dysbalanced bacteria is proved associated with abnormal scalp conditions, and thus, a scalp care solution can be developed by regulating bacteria. Moreover, they found that targeting on scalp commensal bacterial is more effective than the traditional solutions; and it gives scalp a holistic remedy at once not only altering microbial communities but also improving physiological conditions to reach a balanced microenvironment to improve the scalp condition holistically, with moisturized barrier, soothed redness, and relieved scalp itch.
- Based on the above findings, the inventors conducted a lot of experiments and an intensive study, and surprisingly obtained a compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule, which can be used as a microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp.
- Accordingly, the present invention provides a use of a compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule, as a microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp.
- The present invention also provides a method of screening a compound or mixture for a microbiome regulator on scalp, comprising:
- -- testing Minimum Inhibitory Concentrations of the compound or mixture towards Cutibacterium acnes and Staphylococcus epidermidis, respectively;
- -- obtaining direct values of Minimum Inhibitory Concentration (Cutibacterium acnes) and Minimum Inhibitory Concentration (Staphylococcus epidermidis) of the compound or mixture, respectively;
- -- obtaining a calculated value of ( (Minimum Inhibitory Concentration (Cutibacterium acnes) /Minimum Inhibitory Concentration (Staphylococcus epidermidis) ) of the compound or mixture from the above direct values; and
- -- determining that the compound or mixture is suitable as a microbiome regulator on scalp, if the calculated value <1; whereas the compound or mixture is not suitable as a microbiome regulator on scalp, if the calculated value > = 1.
- Furthermore, the present invention provides a shampoo or scalp serum composition, comprising a microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp, which is a compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule, or the mixture thereof.
- The use and the shampoo or scalp serum composition according to the present invention not only cure symptoms in a short period but also remedy scalp to improve scalp overall condition in a long period. Moreover, by the method according to the present invention, the use and the shampoo or scalp serum which not only cure symptoms in a short period but also remedy scalp to improve scalp overall condition in a long period can be obtained.
- It is to be understood by one of ordinary skill in the art that the present discussion is a description of exemplary embodiments only, and is not intended as limiting the broader aspects of the present invention. Each aspect so described may be combined with any other aspect or aspects unless clearly indicated to the contrary. In particular, any feature indicated as being preferred or advantageous may be combined with any other feature or features indicated as being preferred or advantageous.
- Unless specified otherwise, in the context of the present invention, the terms used are to be construed in accordance with the following definitions.
- Unless specified otherwise, all wt%values quoted herein are percentages by weight based on total weight of the shampoo or scalp serum composition.
- Unless specified otherwise, as used herein, the singular forms “a” , “an” and “the” include both singular and plural referents.
- The terms “comprising” and “comprises” as used herein are synonymous with “including” , “includes” or “containing” , “contains” , and are inclusive or open-ended and do not exclude additional, non-recited members, elements or process steps.
- Unless specified otherwise, the recitation of numerical end points includes all numbers and fractions subsumed within the respective ranges, as well as the recited end points.
- Unless specified otherwise, as used herein, terms “microbiome regulator” and “microbiome balancer” are interchangeable, and they both refer to a substance which can regulate the ecological communities of commensal bacteria on the scalp, up-regulating good bacteria and down-regulating bad ones, and then in consequence improve the scalp condition holistically, with moisturized barrier, soothed redness, and relieved scalp itch.
- Unless specified otherwise, as used therein, a term “Minimum Inhibitory Concentration” can be abbreviated to “MIC” ; and it refers to the minimum concentration of a chemical that will inhibit the growth of a microorganism, and is usually reported as μg/ml or mg/ml for a solid compound, and as percentage value for a liquid compound. The “Minimum Inhibitory Concentration” in the present invention is measured according to the method as described in the Examples of the present description.
- The expression “Minimum Inhibitory Concentration (Cutibacterium acnes) of the compound or mixture” and “Minimum Inhibitory Concentrations of the compound or mixture towards Cutibacterium acnes” are interchangeable, and they both refer to the minimum concentration of the compound or mixture that will inhibit the growth of Cutibacterium acnes. The expression “Minimum Inhibitory Concentration (Staphylococcus epidermidis) of the compound or mixture” and “Minimum Inhibitory Concentrations of the compound or mixture towards Staphylococcus epidermidis” are interchangeable, and they both refer to the minimum concentration of the compound or mixture that will inhibit the growth of Staphylococcus epidermidis.
- Unless otherwise defined, all terms used in the disclosing the invention, including technical and scientific terms, have the meaning as commonly understood by one of the ordinary skill in the art to which this invention belongs.
- According to the present invention, surprisingly, a compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule, can be used as a microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp.
- In an aspect of the present invention, the compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule has a ratio of Minimum Inhibitory Concentration (Cutibacterium acnes) to Minimum Inhibitory Concentration (Staphylococcus epidermidis) <1. Preferably, the ratio of Minimum Inhibitory Concentration (Cutibacterium acnes) to Minimum Inhibitory Concentration (Staphylococcus epidermidis) is not more than 0.9, not more than 0.8, not more than 0.7, not more than 0.6, not more than 0.5, not more than 0.4, not more than 0.3, not more than 0.25, or not more than 0.20. Preferably, the ratio of Minimum Inhibitory Concentration (Cutibacterium acnes) to Minimum Inhibitory Concentration (Staphylococcus epidermidis) is at least 0.01, at least 0.02, or at least 0.025. In a preferred embodiment, the ratio of Minimum Inhibitory Concentration (Cutibacterium acnes) to Minimum Inhibitory Concentration (Staphylococcus epidermidis) is in a range of 0.01 to 1, preferably 0.02 to 0.9, more preferably 0.025 to 0.5. If the ratio of Minimum Inhibitory Concentration (Cutibacterium acnes) to Minimum Inhibitory Concentration (Staphylococcus epidermidis) is equal to or higher than 1, the compound cannot be effectively used as the microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp. If the ratio of Minimum Inhibitory Concentration (Cutibacterium acnes) to Minimum Inhibitory Concentration (Staphylococcus epidermidis) is less than 0.01, it will lead to an imbalance of the bacterium.
- In another aspect of the present invention, the compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule contains two, three, four or five alcoholic hydroxyl groups or phenolic hydroxyl groups per molecule. Preferably, the compound contains two, three or four alcoholic hydroxyl groups or phenolic hydroxyl groups per molecule. More preferably, the compound contains two alcoholic hydroxyl groups or four phenolic hydroxyl groups per molecule.
- Preferably, the compound is selected from the group consisting of propylene glycol, butylene glycol, pentylene glycol, hexylene glycol, caprylyl glycol, decylene glycol, dipropylene glycol, glycerin, ethylhexylglycerin and phloretin. Among others, more preferably, the compound is selected from the group consisting of pentylene glycol, hexylene glycol, caprylyl glycol, decylene glycol, ethylhexylglycerin and phloretin.
- Examples of commercially available compound according to the present invention include, for example, SymClariol, which is decylene glycol available from Symrise Company; Caprylyl Glycol, which is available from Symrise Company; Pentylene Glycol, which is available from Symrise Company; EthylHexylGlycerin, which is available from Schülke &Mayr GmbH; and Phloretin, which is Phloretin available from Symrise Company.
- According to the present invention, a method of screening a compound or mixture for a microbiome regulator on scalp comprises:
- -- testing Minimum Inhibitory Concentrations of the compound or mixture towards Cutibacterium acnes and Staphylococcus epidermidis, respectively;
- -- obtaining direct values of Minimum Inhibitory Concentration (Cutibacterium acnes) and Minimum Inhibitory Concentration (Staphylococcus epidermidis) of the compound or mixture, respectively;
- -- obtaining a calculated value of ( (Minimum Inhibitory Concentration (Cutibacterium acnes) /Minimum Inhibitory Concentration (Staphylococcus epidermidis) ) of the compound or mixture from the above direct values; and
- -- determining that the compound or mixture is suitable as a microbiome regulator on scalp, if the calculated value <1; whereas the compound or mixture is not suitable as a microbiome regulator on scalp, if the calculated value > = 1.
- As mentioned above, all the Minimum Inhibitory Concentrations are measured according to the method as described in the Examples of the present description.
- According to the present invention, a shampoo or scalp serum composition comprises a microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp, which is a compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule, or the mixture thereof.
- In an aspect of the shampoo or scalp serum composition according to the present invention, the mircobiome regulator is the compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule as defined above, or the mixture thereof.
- In another aspect of the present invention, based on the total weight of the shampoo or scalp serum composition, the amount of the microbiome regulator is at least 0.01 wt. %, at least 0.05%, at least 0.1 wt. %, at least 0.5%, or at least 1.0%. On the other hand, based on the total weight of the shampoo or scalp serum composition, the amount of the microbiome regulator is not more than 15.0 wt. %, not more than 14 wt. %, not more than 13 wt. %, not more than 12 wt. %, not more than 11 wt. %, or not more than 10 wt. %. In a preferred embodiment, the amount of the microbiome regulator is within a range of 0.01 wt. %to 15.0 wt. %, preferably 0.5 wt. %to 13 wt. %, more preferably 1 wt. %to 10 wt. %, based on the total weight of the shampoo or scalp serum composition. If the amount of the microbiome regulator is less than 0.01 wt. %based on the total weight of the shampoo or scalp serum composition, the composition cannot effectively cure symptoms in a short period and remedy scalp to improve scalp overall condition in a long period. If the amount of the microbiome regulator is higher than 15.0 wt. %based on the total weight of the shampoo or scalp serum composition, the formulation will be not stable in a long term and the efficacy delivery of the microbiome regulator will be lower.
- In a preferred embodiment, the microbiome regulator contains two alcoholic hydroxyl groups or four phenolic hydroxyl groups per molecule. In an embodiment of the present invention, the microbiome regulator is selected from the group consisting of propylene glycol, butylene glycol, pentylene glycol, hexylene glycol, caprylyl glycol, decylene glycol, dipropylene glycol, glycerin, ethylhexylglycerin and phloretin. In another embodiment of the present invention, the microbiome regulator is selected from the group consisting of pentylene glycol, hexylene glycol, caprylyl glycol, decylene glycol, ethylhexylglycerin, glycerin and phloretin. Preferably, the microbiome regulator contains ethylhexylglycerin, decylene glycol and glycerin. More preferably, the microbiome regulator further contains 1, 2-hexanediol and/or propylene glycol.
- The shampoo or scalp serum composition according to the present invention may be formulated to contain one or more additional components or additives besides those described above. For example, the shampoo composition may additionally comprise a solvent, a surfactant, a thickener, a hair conditioning agent and/or PH adjuster; and the scalp serum composition may additionally comprise a solvent, a thickener, a hair conditioning agent and/or PH adjuster.
- The solvent which can be used in the practice of the present invention can be any solvent conventionally used in the shampoo or scalp serum, and it is preferably Aqua.
- The surfactants which can be used in the practice of the present invention include, but are not limited to:
- Anionic surfactants, for example, Acylated Amino Acids, such as Sodium Lauroyl Sarcosinate, and TEA-Cocoyl Glutamate; Carboxylic Acids &salts, such as Sodium Laureth-13 Carboxylate; Sulfonic Acid derivatives, such as Sodium Methyl Cocoyl Taurate, Sodium Cocoyl Isethionate, and Sodium C14-16 Olefin Sulfonate; Sulfuric Acid derivatives, such as Sodium Laureth Sulfate, and Ammonium Lauryl Sulfate;
- Nonionic surfactants, for example, Alkyl Glucosides, such as Coco-Glucoside and Lauryl Glucoside; and Amine oxides, such as Lauramine Oxide;
- Amphoteric surfactants, for example, Alkyl amido Alkyl Amines, such as Sodium Lauroamphoacetate; and Alkyl Betaines, such as Cocamidopropyl betaine, Coco-betaine and coco-hydroxysultaine.
- Examples of thickener which can be used for shampoo in the practice of the present invention include, but are not limited to PEG/PPG-120/10 Trimethylolpropane Trioleate, PEG-120 Methyl Glucose Dioleate, PEG-150 Distearate, PEG-150 Pentaerythrityl Tetrastearate, PEG-55 Propylene Glycol Oleate, PPG-2 Hydroxyethyl Cocamide, Laureth-2, Cocamide MEA and NaCl.
- Examples of thickener which can be used for serum in the practice of the present invention include, but are not limited to (meth) acrylates, (meth) acrylate crosspolymer, Carbomer, Acrylates/C10-30 Alkyl Acrylate Crosspolymer, Hydroxyethylcellulose, Xanthan Gum and Gellan Gum.
- The PH adjuster which can be used in the practice of the present invention includes, but are not limited to citric acid, lactic acid, sodium hydroxide, and potassium hydroxide.
- The shampoo or scalp serum composition according to the present invention can be made by any appropriate method. For example, the composition can be made by simply mixing all the components together.
- In fact, there are no particular restrictions on the production methods of the shampoo or scalp serum composition of the present invention, as long as the method complies with a conventional method for producing the shampoo or scalp serum composition. For example, the shampoo composition can be produced by a method comprising: heating water in a vessel; mixing the surfactants into the water; heating the mixture until the surfactants are fully dispersed; adding the thickener, hair conditioning agents, microbiome regulator and/or PH adjuster and/or other optional components with continuous stirring until all the components are fully dissolved; and cooling down the final mixture to room temperature. The scalp serum composition can be produced by a method comprising: mixing the thickener, PH adjuster into water with continuous stirring until a homogeneous gel formed without flocs and lumps; adding hair conditioning agents, microbiome regulator and/or other optional components with continuous stirring until all the components are fully dispersed.
- There are also no particular restrictions on the equipment for preparing the shampoo or scalp serum, as long as the equipment can prepare the shampoo or scalp serum composition of the present invention.
- The shampoo or scalp serum composition can be in solid or liquid state. The shampoo can be used in the form of viscous liquid. The serum can be used in either gel form or liquid form.
- The shampoo composition can be applied gently on wet hair, and then the hair is rinsed thoroughly. The shampoo composition may be used as often as desired.
- The scalp serum composition can be applied evenly over scalp, the scalp can be gently massaged for 2-3 minutes until the composition is fully absorbed, and there is no need to rinse or towel-wipe after use. The scalp serum composition may be used whenever there are any discomforts on scalp.
- The compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule according to the present invention can be used as a microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp, and it can be used for not only curing symptoms in a short period but also remedying scalp to improve scalp overall condition in a long period
- By the method of screening a compound or mixture for a microbiome regulator on scalp according to the present invention, the compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule can be obtained.
- The shampoo or scalp serum composition according to the present invention can not only cure symptoms in a short period but also remedies scalp to improve scalp overall condition in a long period.
- Examples
- I. In-vitro Tests
- 1. Tested Samples
- The following samples were tested in the examples.
- SymClariol, which is decylene glycol, was manufactured by Symrise Company.
- Caprylyl Glycol was manufactured by Symrise Company.
- Pentylene Glycol was manufactured by Symrise Company.
- Sensiva SC 50, which is EthylHexylGlycerin was manufactured by Schülke &Mayr GmbH.
- Phloretin, which is Phloretin, was manufactured by Symrise Company.
- LIPACIDE C8G, which is Capryloyl Glycine, was manufactured by Seppic Company.
- Sphingony, which is Sphinganine, was manufactured by Evonik Company.
- Sodium Benzoate was manufactured by Wuhan Youji Industries Co..
- Methyparaben was manufactured by Clariant GmbH.
- BIOXYNE, which is Quaternium-73, was manufactured by Sethic Company.
- Phenoxyethanol was manufactured by BASF.
- 2. Laboratory apparatuses
- The main experimental instruments used in the test process were shown in Table 1.
- Table 1 Main experimental instruments
-
-
- 3. Experimental Materials
- 3.1 Bacterial strain
- Propionibacterium acnes ATCC6919 was purchased from Shanghai Fuxiang Biotechnology Co., Ltd., and Staphylococcus epidermidis ATCC12228 was purchased from the mall North Natronian Biotechnology Co., Ltd.
- 3.2 Experimental test sample and main reagents
- The main experimental reagents used in the test procedure were shown in Table 2.
- Table 2 Main Experimental Reagents
-
-
- 3.3 Medium formulas
- The formulations of the mediums used in the test procedure were shown in Tables 3 and 4.
- Table 3 Staphylococcus epidermidis solid medium formula
-
Reagent name Dosage (unit) Beef extract 3 g Peptone 10 g NaCl 5 g Agar 15 g Distilled water 1 L - Note: The liquid medium (pH =7.0) was not with agar and sterilized at 121 ℃. The solid medium was prepared after being cooled to about 60 ℃ and poured into a clean sterilization plate.
- Table 4 Formula of Cutibacterium acnes liquid medium
-
- Note: The amount of liquid added was about 1/4-1/3 of the anaerobic bottle. Fully purged with nitrogen to remove oxygen and sterilized at 115 ℃ as preparation.
- 4. Experimental methods
- 4.1 Screening for antibacterial activity against Cutibacterium acnes
- 4.1.1 Cutibacterium acnes resuscitation steps:
- a. Took out the lyophilized tube stored in the refrigerator at 4 ℃, wiped the outer wall with 75% ethanol, circled the fine end with a grinding wheel, and opened the lyophilized tube.
- b. Pipetted about 1 mL of sterile water into a lyophilized tube, mixing by pipetting, and injected it into an anaerobic culture flask.
- c. It could be passaged after being cultured for 3 to 5 days in a constant temperature incubator at 37 ℃. After 3 generations of general recovery, it could be used in subsequent experiments.
- 4.1.2 Cutibacterium acnes cryopreservation steps::
- a. Anaerobic cultured 3 d Cutibacterium acnes together with the ground meat-containing medium were transferred to a 50 mL centrifuge tube and centrifuged at 1000 rpm.
- b. Transferred the supernatant remaining after centrifugation to a new 50 mL centrifuge tube, discarded the following minced sediment, centrifuged at 2500 rpm for 5 min, and discarded the supernatant.
- c. Added the autoclaved skim milk, blow evenly, dispensed into a sterile freeze-dried tube about 0.5 mL per lyophilized tube, and wrapped it with 8 layers of sterile gauze.
- d. Stored the lyophilized tube in a refrigerator at 4 ℃ for 15 min, then froze in a -20 ℃ refrigerator for 20 min, then transferred to a -80 ℃ refrigerator overnight.
- e. Transferred the lyophilized tube to the activated freeze dryer and pumped until the sample was dry in the lyophilized tube.
- f. Removed the lyophilized tubes and sealed them with an external flame of alcohol lamp.
- g. Marked the name of the bacteria on the lyophilized tube and the date of freezing, and stored it in a refrigerator at 4 ℃.
- 4.1.3 Cutibacterium acnes culture and passage
- Pipetted about 1 mL of Cutibacterium acnes (cultured for 3~5 days, anaerobically) with a sterile syringe, transferred it to a culture flask containing 1/3 volume of ground beef in fresh broth, and kept anaerobic at 37 ℃. After 3 to 5 days of culture, it could be passaged or used for subsequent experiments.
- 4.1.4 Micro-broth method to test the antibacterial activity of the test substance against Cutibacterium acnes
- The antibacterial activity of the test substance against Cutibacterium acnes was evaluated by the micro-broth dilution method recommended by the American Association of Clinical and Laboratory Standards (see Dej-Adisai S, Meechai I, Puripattanavong J, Kummee S. Antityrosinase and antimicrobial activities from Thai medicinal plants. Arch Pharm Res, 2014, 37 (4) : 473-483; and Guo M, Lu Y, Yang J, Zhao X, Lu Y. Inhibitory effects of Schisandra chinensis extract on acne-related inflammation and UVB-induced photoageing. Pharmaceutical Biology, 2016, 54 (12) : 2987-2994) to determine the minimum inhibitory concentration (MIC) of the test substance against Cutibacterium acnes.
- Investigated the MIC of Cutibacterium acnes at different concentrations of the test substance. The specific steps were as follows:
- a. Centrifugally harvest Cutibacterium acnes (cultured for 3 d, anaerobically) , washed 3 times with PBS buffer, and adjusted the bacterial concentration to 2 × 10 8 CFU /mL, then diluted to 1.25 × 10 6 CFU /mL with fresh cooked meat medium.
- b. Diluted the test substance with the meat medium to a concentration of 2 times the final concentration.
- c. Added 100 μL of medium containing different concentrations of test substance and 100 μL of medium containing Cutibacterium acnes to a sterile 96-well plate, and treated with penicillin and chloramphenicol as a positive control, with no drug treatment as negative control, without Cutibacterium acnes containing medium alone as a blank control. Cultured anaerobically at 37 ℃ for 24 h.
- d. After the end of the culture, the growth inhibition of Cutibacterium acnes was detected by a microplate reader evaluating the OD value (absorbance) at 600 nm. The minimum concentration of the test substance when Cutibacterium acnes growth was completely inhibited was the minimum inhibitory concentration (MIC) .
- 4.2 Screening for antibacterial activity against Staphylococcus epidermidis
- 4.2.1 Staphylococcus epidermis resuscitation, subculture steps:
- On the ultra-clean workbench, the lyophilized powder was taken out from the refrigerator, resuscitated according to the species instructions, and cultured at 105 rpm with a constant temperature of 37 ℃. After 1-2 days of culture, transferred a small amount of bacterial liquid to fresh liquid medium. Generally cultured for 3 generations after resuscitation, it could be used for subsequent experiments.
- 4.2.2 Staphylococcus epidermis cryopreservation steps:
- Took the solution after 1 day of culture, add 20%of the final volume of glycerin, mixed and froze it to -20 ℃.
- 4.2.3 Preparation of bacterial suspension:
- The bacterial suspension 12 hours after passage was taken, and the bacterial solution was diluted with a new culture solution to a concentration of 1.0×10 4 to 1.0×10 6 CFU/mL.
- 4.2.4 Preparation of the sample to be tested:
- The solid powder sample to be tested was first dissolved in DMSO (completely dissolved) , and then filtered through a 0.22 μM microporous membrane. Diluted twice with the corresponding culture solution, and the appropriate concentration was prepared according to the supplier's recommended amount (DMSO is less than 1%) . The insoluble sample was directly diluted with a medium to prepare a suspension, and the liquid sample was diluted with a medium by volume concentration. The sample solution needed to be prepared right before use. This process needed to be performed aseptically in a clean bench.
- 4.2.5 Loading and measuring:
- Opened the sterile 96-well plate in the clean bench, added 200 μL of the culture solution to each well as the blank control group, added 100 μL of positive drug solution and 100 μL of the bacterial suspension to each well of the positive control group. For the sample solution to be tested, added 100 μL of the bacterial suspension and 100 μL of different concentration test sample solution to each well of the test group. 100 μL of the culture solution and 100 μL of the test sample solution were added to each well of the negative control group. 100 μL of the culture solution and 100 μL of the bacterial suspension were added to each well of the growth control group. The 96-well plate was sealed with a parafilm, wrapped in a fresh-keeping bag, and cultured at 37 ℃, 105 rpm for 24 h, and the OD value at 530 nm was measured by a microplate reader. The positive drug is: penicillin. Three sets of parallel experiments were conducted. The minimum inhibitory concentration (MIC) was defined as the lowest concentration of the test substance at which bacterial growth was completely inhibited.
- According to the above methods, the direct values of Minimum Inhibitory Concentration (Cutibacterium acnes) and Minimum Inhibitory Concentration (Staphylococcus epidermidis) of all the tested samples were obtained. Then, the calculated values of ( (Minimum Inhibitory Concentration (Cutibacterium acnes) /Minimum Inhibitory Concentration (Staphylococcus epidermidis) ) of all the tested samples were obtained. If the calculated value of a sample is less than 1, the sample is suitable as a microbiome regulator on scalp; whereas if the calculated value of a sample is equal to or higher than 1, the sample is not suitable as a microbiome regulator.
- The direct values and the calculated values of all the tested samples were shown in Table 5.
- Table 5
-
- As shown in Table 1, it is obvious that the compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule, according to the present invention can be used as a microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp. In particular, it can be seen that the compounds of Inventive Examples 1-5 (all of which contain two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule according to the present invention) can be used as microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp, whereas the compounds of Comparative Examples 1-6 (which contain one hydroxyl group, eighteen carbon atoms, no hydroxyl group, one hydroxyl group, no hydroxyl group and twenty-three carbon atoms, and one hydroxyl group per molecule, respectively) cannot be used as microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp.
- II. Clinical Tests
- The following two compositions as shown in Tables 6 and 7 were tested.
- Table 6 Shampoo composition of Example 1
-
- Table 7 Scalp serum composition of Example 2
-
-
- 20 subjects troubled with scalp itchiness but not diagnosed of dandruff had completed a 6-day clinical study.
- For the composition of Example 1, 10 subjects were shampooed with the composition of Example 1 every the other day, that is three times shampoos in total. For each shampooing, the composition of Example 1 was applied twice, 6g of each application.
- For the composition of Example 2, which is a leave-on serum, it was applied on blow-dried scalp of another 10 subjects after washing, 4 g each subject. During the course, the composition of Example 2 was also applied three times in total.
- The evaluation of scalp condition was implemented before and after the 6-day treatment. From the results, it can be seen that the compositions according to the present invention not only cure scalp itchiness &redness to the subject in this short period but also remedy scalp with improved scalp moisture and reduced inflammation securing a healthy scalp condition in a long run.
Claims (15)
- Use of a compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule, as a microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp.
- The use according to claim 1, wherein the compound has a ratio of Minimum Inhibitory Concentration (Cutibacterium acnes) to Minimum Inhibitory Concentration (Staphylococcus epidermidis) <1.
- The use according to claim 1 or 2, wherein the compound contains two alcoholic hydroxyl groups or four phenolic hydroxyl groups per molecule.
- The use according to claim 1 or 2, wherein the compound is selected from the group consisting of propylene glycol, butylene glycol, pentylene glycol, hexylene glycol, caprylyl glycol, decylene glycol, dipropylene glycol, glycerin, ethylhexylglycerin and phloretin.
- The use according to any one of claims 1 to 4, wherein the compound is selected from the group consisting of pentylene glycol, hexylene glycol, caprylyl glycol, decylene glycol, ethylhexylglycerin and phloretin.
- A method of screening a compound or mixture for a microbiome regulator on scalp, comprising:-- testing Minimum Inhibitory Concentrations of the compound or mixture towards Cutibacterium acnes and Staphylococcus epidermidis, respectively;-- obtaining direct values of Minimum Inhibitory Concentration (Cutibacterium acnes) and Minimum Inhibitory Concentration (Staphylococcus epidermidis) of the compound or mixture, respectively;-- obtaining a calculated value of ( (Minimum Inhibitory Concentration (Cutibacterium acnes) /Minimum Inhibitory Concentration (Staphylococcus epidermidis) ) of the compound or mixture from the above direct values; and-- determining that the compound or mixture is suitable as a microbiome regulator on scalp, if the calculated value <1; whereas the compound or mixture is not suitable as a microbiome regulator on scalp, if the calculated value > = 1.
- A shampoo or scalp serum composition, comprising a microbiome regulator for selectively inhibiting Cutibacterium acnes and Staphylococcus epidermidis on scalp, which is a compound containing two or more alcoholic hydroxyl groups or phenolic hydroxyl groups and three to sixteen carbon atoms per molecule, and the mixture thereof.
- The composition according to claim 7, wherein the shampoo composition further comprises a solvent, a surfactant, a thickener, a hair conditioning agent and/or a PH adjuster.
- The composition according to claim 7, wherein the scalp serum composition further comprises a solvent, a thickener, a hair conditioning agent and/or a PH adjuster.
- The composition according to any one of claims 7 to 9, wherein the amount of the microbiome regulator is within a range of 0.005 wt. %to 15.0 wt. %, preferably 5 wt. %to 10 wt. %, based on the total weight of the composition.
- The composition according to any one of claims 7 to 9, wherein the microbiome regulator contains two alcoholic hydroxyl groups or four phenolic hydroxyl groups per molecule.
- The composition according to any one of claims 7 to 9, wherein the microbiome regulator is selected from the group consisting of propylene glycol, butylene glycol, pentylene glycol, hexylene glycol, caprylyl glycol, decylene glycol, dipropylene glycol, glycerin, ethylhexylglycerin and phloretin.
- The composition according to any one of claims 7 to 9, wherein the microbiome regulator is selected from the group consisting of pentylene glycol, hexylene glycol, caprylyl glycol, decylene glycol, ethylhexylglycerin, glycerin and phloretin.
- The composition according to any one of claims 7 to 9, wherein the microbiome regulator contains ethylhexylglycerin, decylene glycol and glycerin.
- The composition according to claim 14, wherein the microbiome regulator further contains 1, 2-hexanediol and/or propylene glycol.
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