EP4149997A1 - Biopolymer production with fraction of whey components and biodegradable/biocompatible membrane fabrication method - Google Patents

Biopolymer production with fraction of whey components and biodegradable/biocompatible membrane fabrication method

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Publication number
EP4149997A1
EP4149997A1 EP21804627.4A EP21804627A EP4149997A1 EP 4149997 A1 EP4149997 A1 EP 4149997A1 EP 21804627 A EP21804627 A EP 21804627A EP 4149997 A1 EP4149997 A1 EP 4149997A1
Authority
EP
European Patent Office
Prior art keywords
whey
membrane
protein
kda
lactose
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP21804627.4A
Other languages
German (de)
French (fr)
Other versions
EP4149997A4 (en
Inventor
Derya YUKSEL IMER
Hayrettin Guclu INSEL
Mehmet DILAVER
Sama A. AL-MUTWALLI
Digdem GUVEN
Emine COKGOR
Gulsum Emel ZENGIN BALCI
Elifnur GEZMIS YAVUZ
Mustafa N. TAHER
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Istanbul Teknik Universitesi ITU
Original Assignee
Istanbul Teknik Universitesi ITU
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Istanbul Teknik Universitesi ITU filed Critical Istanbul Teknik Universitesi ITU
Publication of EP4149997A1 publication Critical patent/EP4149997A1/en
Publication of EP4149997A4 publication Critical patent/EP4149997A4/en
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
    • C08J5/18—Manufacture of films or sheets
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/001—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste
    • A23J1/004—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste from waste products of dairy plant
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/04—Animal proteins
    • A23J3/08—Dairy proteins
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D67/00—Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
    • B01D67/0002—Organic membrane manufacture
    • B01D67/0004—Organic membrane manufacture by agglomeration of particles
    • B01D67/00042—Organic membrane manufacture by agglomeration of particles by deposition of fibres, nanofibres or nanofibrils
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/08—Polysaccharides
    • B01D71/10—Cellulose; Modified cellulose
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B01D71/74—Natural macromolecular material or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L89/00—Compositions of proteins; Compositions of derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
    • D—TEXTILES; PAPER
    • D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
    • D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
    • D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
    • D04H1/40—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties
    • D04H1/42—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres from fleeces or layers composed of fibres without existing or potential cohesive properties characterised by the use of certain kinds of fibres insofar as this use has no preponderant influence on the consolidation of the fleece
    • D04H1/425—Cellulose series
    • D—TEXTILES; PAPER
    • D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
    • D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
    • D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
    • D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
    • D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
    • D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2311/00—Details relating to membrane separation process operations and control
    • B01D2311/26—Further operations combined with membrane separation processes
    • B01D2311/2688—Biological processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2311/00—Details relating to membrane separation process operations and control
    • B01D2311/26—Further operations combined with membrane separation processes
    • B01D2311/2696—Catalytic reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/66—Biodegradability of parts of the module
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2313/00—Details relating to membrane modules or apparatus
    • B01D2313/68—Biocompatibility of parts of the module
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2323/00—Details relating to membrane preparation
    • B01D2323/26—Spraying processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2323/00—Details relating to membrane preparation
    • B01D2323/39—Electrospinning
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
    • B01D61/14—Ultrafiltration; Microfiltration
    • B01D61/145—Ultrafiltration

Definitions

  • the invention relates to the production of biopolymer with a fraction of whey components by cyclic approach via an enzymatic extractive ceramic membrane reactor (EECeMR) and a process for the fabrication of biodegradable/biocompatible membranes.
  • EECeMR enzymatic extractive ceramic membrane reactor
  • Korean patent specification number KR20160069949 mentions a membrane for periodontal regeneration that provides a space for osteogenesis by preventing soft tissue cells from invading a bone graft material.
  • a membrane containing polyhydroxybutyrate in its content is mentioned.
  • Another object of the present invention is to fabricate a membrane which prevents environmental pollution by its ingredients.
  • Figure 1 is the overall view of the vessel used in the membrane fabrication process.
  • Figure 2 is the schematic view of the process steps in the membrane fabrication.
  • the invention relates to a process for the fabrication of membranes from industrial waste, and more specifically to a new biodegradable/biocompatible process for the fabrication of membranes from the materials produced as waste during whey powder production.
  • lactose waste is enabled in whey processing plants by producing glucose+galactose feedstock from lactose waste with EECeMR.
  • the enzyme and substrate flux on two different membrane surfaces in the EECeMR design eliminates the problem of enzyme being damaged by the cleaning chemicals when the membrane performance/activity is reduced and chemical cleaning of the membrane occurs in classical way.
  • the readily degradable glucose+galactose solution produced in the EECeMR process is converted into a biodegradable organic substance (PHB)by using it as carbon source in the bioreactor designed for biopolymer production.
  • PHB biodegradable organic substance
  • the extraction of polyhydroxybutyrate (PHB) is about 6-8% (as mass) in the process in the fermentation reactor.
  • Glucose+galactose mixture is converted to volatile fatty acids (VFAs) in the first stage of fermentation and then converted to PHB granules in bacteria via sequencing batch reactor system.
  • Glucose+galactose is converted into the polyhydroxybutyrate (PHB) at the end of the two-step bioreaction.
  • the production of biodegradable/biocompatible membranes with the waste-derived biopolymer (PHB) is shown in the last step of the process diagram.
  • the disclosed method of the invention comprises the steps of
  • the process is started by feeding whey permeate with low protein and high lactose content from whey production plants and filling vessel I (1). Then feed with lactose is continuously provided, which is fed into the tubular ceramic membrane module (3). After the lactose is converted into a glucose+galactose solution by passing through the inner surface of the tubular membrane (4) with the flow and reacting with the enzyme on the outer surface, and after this product is collected in the pore space of the module, so that from here it enters the to vessel II (2), it is envisaged that the glucose+galactose solution is fed as a carbon source from vessel II (2) to the fermenters where PHB production takes place.
  • the efficiency of the disclosed membrane of the invention was first tested in a suspended solution. A hydrolysis value of 95% was obtained with a concentration of 40 g/L lactose and a concentration of 5 g/L enzyme within 6 hours. With an enzymatic ceramic membrane immobilized in the direction of substrate flow, a hydrolysis efficiency of 40% was achieved in similar time periods.
  • the major drawback with this method is that flow reduces enzyme activity and flow hydraulics affect the hydrolysis reaction. In the EECeMR configuration, there is membrane layer between the contact of the enzyme and the substrate (Lactose) flow. Therefore, the immobilized enzymes are not damaged by the flow hydraulics.
  • the enzyme is completely chemically linked to the outer surface of the membrane, not in free form and when the substrate passes the membrane, a reaction takes place at the surface and the substrate turns into the product. Therefore, the substrate-enzyme -product phases are separated by a single membrane layer.

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Manufacturing & Machinery (AREA)
  • Polymers & Plastics (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Materials Engineering (AREA)
  • General Health & Medical Sciences (AREA)
  • Textile Engineering (AREA)
  • Food Science & Technology (AREA)
  • Nanotechnology (AREA)
  • Nutrition Science (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the production of biopolymer with a fraction of whey components by cyclic approach via an enzymatic extractive ceramic membrane reactor (EECeMR) and a process for the fabrication of biodegradable/biocompatible membranes.

Description

BIOPOLYMER PRODUCTION WITH FRACTION OF WHEY COMPONENTS AND BIODEGRADABLE/BIOCOMPATIBLE MEMBRANE FABRICATION
METHOD
Technical Field
The invention relates to the production of biopolymer with a fraction of whey components by cyclic approach via an enzymatic extractive ceramic membrane reactor (EECeMR) and a process for the fabrication of biodegradable/biocompatible membranes.
Prior Art
The production of protein rich powder from whey is an industrially applied process. Membrane systems, especially ultrafiltration (UF) and nanofiltration, are widely used in this field. In this production, lactose collects in the permeate of the membrane system. This permeate (whey permeate) is a frequently unwanted/unused permeate in the industry and is discharged. The organic content of this permeate, which contains lactose, is very high and its discharge leads to environmental pollution. Lactose is usually removed from milk enzymatically. However, the usual common application is the suspended use of enzymes or enzyme coating on the classical membrane surface in the direction of flow.
In general, protein powder is of interest in the plants/production facilities where whey is used, other residues are considered waste and are not used. Recovery of lactose and recovery of valuable products (such as lactic acid) are difficult costly in practice. Also, complete recovery of lactose is not be possible as it contains mineral components, it requires additional technological steps. The contact of the flux and the enzyme causes the decrease of the catalytic activity of the enzyme in the long-term use for the removal of lactose with the immobilized enzyme, especially for the practice in the dairy industry. The long-term usability performance of the enzyme coated material decreases. When the usability of the enzyme decreases, the specific separation cannot be completed and causes fouling in the membrane. In the technique of paten, membrane fabrication was carried out with the fraction of whey components.
The American patent specification numbered US20030004299A1, mentions the biological production of polymers and the biological production of polyhydroxy alkanoates.
Korean patent specification number KR20160069949 mentions a membrane for periodontal regeneration that provides a space for osteogenesis by preventing soft tissue cells from invading a bone graft material. A membrane containing polyhydroxybutyrate in its content is mentioned.
In the analysis of membrane fabrication in the prior art, (i) the one-step enzymatic conversion of wastewater containing a large amount of lactose into a glucose and/or galactose solution in whey production plants, (ii) the production of biopolymer with this solution and (iii) a new biodegradable/biocompatible membrane fabrication process using whey protein with the produced biopolymer by combination with various additives need to be developed.
The Aims of the Invention
It is an object of the present invention to provide an environmentally friendly process for membrane fabrication from waste materials in the production of whey powder.
Another object of the present invention is to fabricate a membrane which prevents environmental pollution by its ingredients.
Detailed Description of the Invention
The membrane fabrication process realized to achieve the objectives of the present invention is illustrated in the accompanying figures. These figures;
Figure 1: is the overall view of the vessel used in the membrane fabrication process.
Figure 2: is the schematic view of the process steps in the membrane fabrication.
The elements in the figures are numbered respectively, the correspondences of these numbers are given below.
1. Vessel I
2. Vessel II
3. Membrane module
4. Tubular ceramic membrane
5. Pump
The invention relates to a process for the fabrication of membranes from industrial waste, and more specifically to a new biodegradable/biocompatible process for the fabrication of membranes from the materials produced as waste during whey powder production.
The reuse of lactose waste is enabled in whey processing plants by producing glucose+galactose feedstock from lactose waste with EECeMR. The enzyme and substrate flux on two different membrane surfaces in the EECeMR design eliminates the problem of enzyme being damaged by the cleaning chemicals when the membrane performance/activity is reduced and chemical cleaning of the membrane occurs in classical way.
The readily degradable glucose+galactose solution produced in the EECeMR process is converted into a biodegradable organic substance (PHB)by using it as carbon source in the bioreactor designed for biopolymer production. The extraction of polyhydroxybutyrate (PHB) is about 6-8% (as mass) in the process in the fermentation reactor. By converting the lactose into glucose+galactose at 90% ratio with EECeMR system, this new solution is used in polyhydroxybutyrate (PHB) production by bacteria. Glucose+galactose mixture is converted to volatile fatty acids (VFAs) in the first stage of fermentation and then converted to PHB granules in bacteria via sequencing batch reactor system. Glucose+galactose is converted into the polyhydroxybutyrate (PHB) at the end of the two-step bioreaction. The production of biodegradable/biocompatible membranes with the waste-derived biopolymer (PHB) is shown in the last step of the process diagram.
The disclosed method of the invention comprises the steps of
• Providing whey comprising 0.9% protein, 4.8% lactose, 0.05% fat, 0.5% mineral and 93% water by mass (100),
• Providing a recovery of the whey feed at 70% by volume under an UF (ultrafiltration) membrane (UF-10 kDa) having a 10 kDa (kilodalton) molecular weight cut-off value (110),
• Obtaining whey concentrate via UF-10 kDa (120),
• Obtaining 30-35% (as mass) protein from whey concentrate as WPC-30 powder in spray dryer (130),
• Obtaining lactose+mineral solution in permeate of UF-10 kDa (140),
• Sending the obtained lactose solution to enzymatic extractive ceramic membrane reactor (EECeMR) (150),
• Obtaining glucose+galactose+mineral solution as a result of the operation (160),
• Introducing the glucose+galactose+mineral solution into the fermentation reactor (170),
• Extraction of polyhydroxybutyrate (PHB) after the process in the fermentation reactor (180),
• Obtaining a nanofiber membrane by mixing compounds obtained by obtaining 30%- 35% protein from concentrate of UF-10 kDa with WPC-30 powder in spray dryer (130) and by polyhydroxybutyrate (PHB) extraction (180) after the process in fermentation reactor (190).
In membrane fabrication according to the invention, the process is started by feeding whey permeate with low protein and high lactose content from whey production plants and filling vessel I (1). Then feed with lactose is continuously provided, which is fed into the tubular ceramic membrane module (3). After the lactose is converted into a glucose+galactose solution by passing through the inner surface of the tubular membrane (4) with the flow and reacting with the enzyme on the outer surface, and after this product is collected in the pore space of the module, so that from here it enters the to vessel II (2), it is envisaged that the glucose+galactose solution is fed as a carbon source from vessel II (2) to the fermenters where PHB production takes place. After PHB leaves the biological environment in the fermenter and is mixed with whey protein powder and cellulose nanofibrils, solutions are prepared in different ratios and a nanofiber membrane is fabricated from the solutions containing PHB/protein/cellulose nanofibril using an electrospinning device; a biodegradable nanofiber membrane is obtained.
The efficiency of the disclosed membrane of the invention was first tested in a suspended solution. A hydrolysis value of 95% was obtained with a concentration of 40 g/L lactose and a concentration of 5 g/L enzyme within 6 hours. With an enzymatic ceramic membrane immobilized in the direction of substrate flow, a hydrolysis efficiency of 40% was achieved in similar time periods. The major drawback with this method is that flow reduces enzyme activity and flow hydraulics affect the hydrolysis reaction. In the EECeMR configuration, there is membrane layer between the contact of the enzyme and the substrate (Lactose) flow. Therefore, the immobilized enzymes are not damaged by the flow hydraulics. During the transport of the lactose through the porous stmcture, the contact with the enzyme on the product side is given and the efficiency of hydrolysis reaches a maximum. Continuous operation becomes possible by circulating the non-hydrolyzed lactose continuously with the inner circuit. Another advantage of this configuration is that during chemical cleaning after lactose fouling of the inner membrane, the enzyme is not damaged by this cleaning procedure. When the activity decreases over time, new enzyme solution is supplied by the enzyme-coated product.
The enzyme is completely chemically linked to the outer surface of the membrane, not in free form and when the substrate passes the membrane, a reaction takes place at the surface and the substrate turns into the product. Therefore, the substrate-enzyme -product phases are separated by a single membrane layer.

Claims

1. The invention relates to a process for fabricating a biodegradable/biocompatible membrane, characterized in that it comprises the following steps;
• Providing whey containing 0.9% protein, 4.8% lactose, 0.05% fat, 0.5% mineral and 93% water by mass (100),
• Providing a recovery of delivered whey of 70% by UF-10 kDa (110),
• Obtaining a whey concentrate by UF-10 kDa (120),
• Obtaining a whey protein powder (WPC-30 powder) having 30-35% protein (as mass) by drying whey concentrate of UF-10 kDa with a spray dryer (130),
• Obtaining lactose+mineral solution in permeate of UF-10 kDa (140),
• Sending obtained lactose+mineral solution into the enzymatic extractive ceramic membrane reactor (EECeMR) (150),
• Obtaining glucose+galactose+mineral solution as a result of enzymatic operation (160),
• Introducing the glucose+galactose+mineral solution into the fermentation reactor (170),
• Performing the extraction of polyhydroxybutyrate (PHB) after the process in the fermentation reactor (180),
• Obtaining a nanofiber membrane by mixing compounds having 30-35% protein (as mass) from whey concentrate with UF-10 kDa in a spray dryer (130) and by polyhydroxybutyrate (PHB) extraction (180) after the process in fermentation reactor (190).
2. It is a membrane fabrication process according to claim 1 ; The polyhydroxybutyrate (PHB) extraction (180) after processing in the fermentation reactor is characterized as containing 6-8% mass PHB (polyhydroxybutyrate).
3. It is a membrane manufacturing process according to claim 1; characterized by converting whey concentrate obtained with UF-10 kDa into whey protein powder (WPC-30 powder) with 30-35% protein content by spray dryer (130) and the amount of this whey protein in membrane fabrication is 2-4% as mass.
EP21804627.4A 2020-05-15 2021-05-06 Biopolymer production with fraction of whey components and biodegradable/biocompatible membrane fabrication method Pending EP4149997A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
TR2020/07641A TR202007641A2 (en) 2020-05-15 2020-05-15 PRODUCTION OF BIOPLASTIC BY FRACTION OF CHEESE Whey COMPONENTS AND METHOD OF PRODUCTION OF BIODEGRADABLE/BIOCOMPATIBLE MEMBRANES
PCT/TR2021/050432 WO2021230845A1 (en) 2020-05-15 2021-05-06 Biopolymer production with fraction of whey components and biodegradable/biocompatible membrane fabrication method

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EP4149997A1 true EP4149997A1 (en) 2023-03-22
EP4149997A4 EP4149997A4 (en) 2024-08-14

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TR (1) TR202007641A2 (en)
WO (1) WO2021230845A1 (en)

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Publication number Priority date Publication date Assignee Title
JP3468955B2 (en) * 1995-12-01 2003-11-25 三菱重工業株式会社 Method for producing lactic acid by microalgae
US6672001B1 (en) * 1998-11-17 2004-01-06 Board Of Regents Of University Of Nebraska Method for mulching an agricultural soil bed using a biodegradable protein material, and a mulched agricultural crop growing plot produced thereby
US20030004299A1 (en) * 2001-03-02 2003-01-02 Regents Of The University Of Minnesota Production of polyhydroxyalkanoates
US9758558B2 (en) * 2010-04-01 2017-09-12 Board Of Supervisors Of Louisiana State University And Agriculture And Mechanical College Whey protein isolate hydrogels and their uses
ES2777941T3 (en) * 2011-07-22 2020-08-06 Pimec Films coated with whey proteins
CN105248836B (en) * 2015-10-13 2019-06-14 东北农业大学 A kind of low irritability PURE WHEY and preparation method thereof based on two steps enzymatic hydrolysis and enzyme membrane reaction

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