EP3948184A1 - Kompaktes raman-mikrospektrometer mit abbildenden planaren digitalen gitterstrukturen - Google Patents
Kompaktes raman-mikrospektrometer mit abbildenden planaren digitalen gitterstrukturenInfo
- Publication number
- EP3948184A1 EP3948184A1 EP20714587.1A EP20714587A EP3948184A1 EP 3948184 A1 EP3948184 A1 EP 3948184A1 EP 20714587 A EP20714587 A EP 20714587A EP 3948184 A1 EP3948184 A1 EP 3948184A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- arrangement
- raman microspectrometer
- microspectrometer
- raman
- designed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001069 Raman spectroscopy Methods 0.000 title claims abstract description 56
- 238000003384 imaging method Methods 0.000 title claims abstract description 20
- 230000003287 optical effect Effects 0.000 claims abstract description 21
- 238000005259 measurement Methods 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 239000000969 carrier Substances 0.000 claims description 3
- 238000012937 correction Methods 0.000 claims description 3
- 239000006185 dispersion Substances 0.000 claims description 3
- 230000008859 change Effects 0.000 claims description 2
- 238000001459 lithography Methods 0.000 claims description 2
- 239000013307 optical fiber Substances 0.000 claims description 2
- 239000000523 sample Substances 0.000 description 12
- 230000005284 excitation Effects 0.000 description 7
- 239000000835 fiber Substances 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 4
- 239000011521 glass Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 239000010408 film Substances 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 230000003595 spectral effect Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000000206 photolithography Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
- G01J3/0218—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using optical fibers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
- G01J3/0237—Adjustable, e.g. focussing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0256—Compact construction
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0272—Handheld
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/08—Beam switching arrangements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/12—Generating the spectrum; Monochromators
- G01J3/18—Generating the spectrum; Monochromators using diffraction elements, e.g. grating
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B5/00—Optical elements other than lenses
- G02B5/18—Diffraction gratings
- G02B5/1842—Gratings for image generation
Definitions
- the present invention relates to a Raman microspectrometer with imaging planar digital grating structures.
- the present invention relates to a Raman microspectrometer device.
- Raman spectroscopy is a fingerprint analysis.
- the excitation laser light reaches the sample through the objective and the objective simultaneously collects the Raman scattered light for the spectral sensor.
- the excitation and collection objective can also be an adjustment-free fiber probe comprising excitation and collection fibers.
- the lens is always used.
- Life sciences require a compact, adjustment-free structure that can switch between incident light and inverse arrangement in one device. This makes it possible to measure the most varied of questions and sample matrices with one and the same device. For a compact device, the components are still as wide as possible
- a sample holder system is integrated as an example, which enables the spectroscopic measurement of microorganisms, cells,
- Spheroids, etc. which are typically prepared in aqueous solution, are allowed.
- a liquid sample e.g. a biological sample, which is located on a glass substrate, is measured with an inverse arrangement (objective from below). The same applies to solid samples.
- Condensation forms on the inside of the cover slip or on the film, an uncontrollable layer of droplets.
- the typical spectral sensor arrangement consists of an entrance slit, an imaging grating and a
- the imaging grating is a massive classical concave lens, usually made of glass, on which the
- Lattice structure is realized with a complex interference exposure and subsequent microstructure technology.
- Incident light operation and can also be changed to
- the present invention thus enables the size and costs to be reduced and at the same time enables simple and reliable integration of the Raman microspectrometer arrangement. This mainly concerns the grating of the Raman microspectrometer arrangement as the main component.
- the object is achieved by an arrangement of a compact, adjustment-free handheld Raman spectrometer for measuring cells / bacteria both on sample carriers and in chambers with aqueous solution and solids. It is advantageously provided that with one and the same structure from a standard incident light arrangement to an inverse one
- Incident light arrangement can be changed during the measurement, and planar digital structures are used for the grating component, which simultaneously realize the imaging and the dispersing function and with known ones
- microlithographic thin-film technologies can be prepared on flat substrates, in the simplest case the
- Microspectrometer to minimize the measurement result.
- the functions mentioned can be implemented with a microstructuring step.
- Other functions that are required for high-performance spectrometers, e.g. the so-called blaze (from English, to blaze sparkle), are with the one
- the blaze determines the efficiency of the grid and is achieved by dividing the planar, curved structures into irregular grid-like structures in lateral substructures. A grid line then consists of several sub-lines of different widths.
- the grating period changes over the grating area
- Lattice bars appear curved and not linear.
- a first aspect of the present invention relates to a Raman microspectrometer arrangement.
- the microspectrometer arrangement comprises an incident light device and a grating component device.
- the incident light device is designed in
- the grating component device comprises planar, digital
- the grating component device is designed as an imaging optical component and / or as a
- Microspectrometer arrangement to be used.
- the Raman microspectrometer Arrangement is designed as an adjustment-free hand-held device, the Raman microspectrometer arrangement preferably being designed for measuring liquid and / or solid samples and being designed for this purpose with sample carriers and / or
- Structures are designed for a correction of
- Image aberration corrections can be implemented which make it possible to use spherical mirrors or lenses that are simpler and more precisely manufacturable instead of toric mirrors or lenses and thus to realize an adjustment-free system.
- spherical mirrors or lenses that are simpler and more precisely manufacturable instead of toric mirrors or lenses and thus to realize an adjustment-free system.
- the aspherical and / or astigmatic grating properties of the planar digital structures can be produced by at least one lithography method, also referred to as a photolithography method.
- Structures are designed to change a dispersion course of the imaging optical component and / or the dispersing optical component.
- the invention provides that the Raman microspectrometer arrangement can be operated or used in a reflective or transmissive manner.
- Structures are designed as chirp grids and one
- the lattice constant of the planar digital structures varies spatially as specified.
- Structures have lattice bars.
- the invention provides that the lattice webs have a curvature.
- the invention provides that a mean grating period is between 1 and 80 mpi, with a lateral subdivision of the height levels being less than 1 mpi.
- Structures have a rounded aperture.
- the Raman microspectrometer arrangement furthermore has a spectrometer head device which has a mirror, a lens and a beam splitter and which is designed to couple in light
- the Raman microspectrometer arrangement is designed to be coupled to an optical fiber bundle
- Implementations of the present invention also include combinations of previously or im
- Fig. 1 a schematic representation of a Raman-
- Fig. 2 a schematic representation of a Raman
- Fig. 3 is a schematic representation of one
- Fig. 4 a schematic representation of excitation
- FIG. 1 shows a schematic representation of a Raman microspectrometer arrangement according to an exemplary embodiment of the present invention.
- the Raman microspectrometer arrangement 100 shown in FIG. 1 comprises an incident light device 10 and a grating component device 20.
- the incident light device 10 is designed to be in
- the incident light device 10 is also designed to be switched between the upright configuration and the inverse configuration during a measurement of the Raman microspectrometer arrangement.
- the grating component device 20 has planar digital structures.
- the grating component device 20 is designed as an imaging optical component and / or as a
- Microspectrometer arrangement to be used.
- FIG. 2 shows a schematic representation of a Raman microspectrometer arrangement according to an exemplary embodiment of the present invention.
- FIG. 3 shows a schematic illustration of a lattice structure according to an exemplary embodiment of FIG.
- Fig. 4 shows a schematic representation of the excitation and collecting obj ective in the form of an adjustment-free fiber probe consisting of excitation and collecting fibers
- Embodiments has been described above, it is not limited to them, but can be modified in many ways.
- the invention can be changed or modified in manifold ways without deviating from the essence of the invention.
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- General Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Optics & Photonics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Spectrometry And Color Measurement (AREA)
Abstract
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102019107924.1A DE102019107924A1 (de) | 2019-03-27 | 2019-03-27 | Kompaktes Raman-Mikrospektrometer mit abbildenden planaren digitalen Gitterstrukturen |
PCT/EP2020/058471 WO2020193677A1 (de) | 2019-03-27 | 2020-03-26 | Kompaktes raman-mikrospektrometer mit abbildenden planaren digitalen gitterstrukturen |
Publications (1)
Publication Number | Publication Date |
---|---|
EP3948184A1 true EP3948184A1 (de) | 2022-02-09 |
Family
ID=70008550
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP20714587.1A Pending EP3948184A1 (de) | 2019-03-27 | 2020-03-26 | Kompaktes raman-mikrospektrometer mit abbildenden planaren digitalen gitterstrukturen |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP3948184A1 (de) |
DE (1) | DE102019107924A1 (de) |
WO (1) | WO2020193677A1 (de) |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5442169A (en) * | 1991-04-26 | 1995-08-15 | Paul Scherrer Institut | Method and apparatus for determining a measuring variable by means of an integrated optical sensor module |
DE102006045624A1 (de) * | 2006-09-27 | 2008-04-03 | Giesecke & Devrient Gmbh | Vorrichtung und Verfahren zur optischen Untersuchung von Wertdokumenten |
DE102012021726A1 (de) * | 2012-11-06 | 2014-05-08 | Celltool Gmbh | Mikroskopsystem und Verfahren zur Datenerfassung |
DE102014018726A1 (de) * | 2014-12-16 | 2016-06-16 | Giesecke & Devrient Gmbh | Vorrichtung und Verfahren zur Prüfung von Merkmalsstoffen |
DE102015001032A1 (de) * | 2015-01-27 | 2016-07-28 | Leibniz-Institut für Photonische Technologien e. V. | Raman-Spektroskopie-Beleuchtungs- und Auslesesystem |
US10458846B2 (en) * | 2015-08-31 | 2019-10-29 | Hewlett-Packard Development Company, L.P. | Spectral microscope |
-
2019
- 2019-03-27 DE DE102019107924.1A patent/DE102019107924A1/de active Pending
-
2020
- 2020-03-26 WO PCT/EP2020/058471 patent/WO2020193677A1/de active Search and Examination
- 2020-03-26 EP EP20714587.1A patent/EP3948184A1/de active Pending
Also Published As
Publication number | Publication date |
---|---|
WO2020193677A1 (de) | 2020-10-01 |
DE102019107924A1 (de) | 2020-10-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP3129818B1 (de) | Anordnung zur lichtblattmikroskopie | |
DE10008006C2 (de) | SPR-Sensor und SPR-Sensoranordnung | |
DE102017211910A1 (de) | Diffraktiver Biosensor | |
DE3874125T2 (de) | Uv-abtastsystem fuer zentrifuge. | |
DE102015201561A1 (de) | Messkopf einer endoskopischen Vorrichtung und Verfahren zur Inspektion und Messung eines Objektes | |
DE102012217419A1 (de) | Analyseverfahren für Röntgenstrahlbeugungsmessdaten | |
DE102016201572A1 (de) | Miniaturspektrometer und Verfahren zum Schalten eines Miniaturspektrometers zwischen Abbildungsmodus und Spektrometermodus | |
DE102015001032A1 (de) | Raman-Spektroskopie-Beleuchtungs- und Auslesesystem | |
DE102013210259A1 (de) | Verfahren zur Messung von Streulicht und Vorrichtung zur Messung von Streulicht | |
EP3948184A1 (de) | Kompaktes raman-mikrospektrometer mit abbildenden planaren digitalen gitterstrukturen | |
DE3152738T1 (de) | Einrichtung zur photometrischen Abtastung von Gelen | |
DE102012108620A1 (de) | Verfahren zum Bestimmen der Weglänge einer Probe und Validierung der damit erhaltenen Messung | |
WO2016066156A2 (de) | Mobile photometrische messvorrichtung und verfahren zur mobilen photometrischen messung an mikrotitierplatten | |
DE102013217157A1 (de) | Analyseverfahren zur Ermittlung der Typen und Konzentrationen biologischer Partikel | |
DE102015108818A1 (de) | Anordnung zur Spektroskopie und Verfahren zur Herstellung der Anordnung | |
EP2247942B1 (de) | Markerfreies chromosomenscreening | |
EP3198323B1 (de) | Vorrichtung zur abbildung einer probe | |
DE102018000307A1 (de) | Statisches Fourier-Transformations-Spektrometer und ein Verfahren zum Betreiben des statischen Fourier-Transformations-Spektrometers | |
AT18115U1 (de) | Vorrichtung zur spektroskopischen Untersuchung von Fluiden | |
DE102018208185A1 (de) | Optisches Element zur Lichtkonzentration und Herstellungsverfahren für ein optisches Element zur Lichtkonzentration | |
EP3770585B1 (de) | Vorrichtung und verfahren zur bestimmung einer stoffkonzentration in einem fluid | |
DE102018126009A1 (de) | Verfahren und Mikroskop zur Bestimmung der Dicke eines Deck- oder Tragglases | |
DE10052165A1 (de) | SPR-Sensorsystem | |
DE102008054737A1 (de) | Mikrolithographische Projektionsbelichtungsanlage sowie Objektiv hierfür | |
DE1234048B (de) | Vorrichtung zur freien Grenzflaechen-Elektrophorese und fuer Diffusionsmessungen |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: UNKNOWN |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE |
|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE |
|
17P | Request for examination filed |
Effective date: 20210930 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
|
DAV | Request for validation of the european patent (deleted) | ||
DAX | Request for extension of the european patent (deleted) | ||
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: EXAMINATION IS IN PROGRESS |
|
17Q | First examination report despatched |
Effective date: 20231109 |