EP3847233A1 - Vorrichtung zum inkubieren von kulturen, verfahren zum inkubieren von kulturen sowie verwendung - Google Patents
Vorrichtung zum inkubieren von kulturen, verfahren zum inkubieren von kulturen sowie verwendungInfo
- Publication number
- EP3847233A1 EP3847233A1 EP19779355.7A EP19779355A EP3847233A1 EP 3847233 A1 EP3847233 A1 EP 3847233A1 EP 19779355 A EP19779355 A EP 19779355A EP 3847233 A1 EP3847233 A1 EP 3847233A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- cultures
- incubation
- culture chamber
- gas
- container
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/36—Means for collection or storage of gas; Gas holders
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/40—Manifolds; Distribution pieces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/54—Constructional details, e.g. recesses, hinges hand portable
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/04—Stirrer or mobile mixing elements with introduction of gas through the stirrer or mixing element
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
Definitions
- the invention relates to a device for incubating cultures, a method for incubating cultures and a use in this regard.
- the cultures are incubated in a gassing manner by means of gaseous CO 2 , which comes directly from corresponding CO 2 pressure cartridges, this CO 2 itself being liquefied and present under high pressure.
- the problem of the present invention is therefore to provide a device for incubating cultures in which precise and safe metering of the respective incubation gas into the culture chambers with regard to the pressure conditions is achieved and is possible.
- the device according to the invention for incubating cultures is one which has a culture chamber which stores the cultures, the cultures being gassed with an incubation gas in use, for example and in particular with CO 2 , the incubation gas source when used to incubate the cultures with the incubation gas is free of liquid incubation gas.
- the incubation gas source when used to incubate the cultures with the incubation gas is free of liquid incubation gas.
- the incubation gas is C0 2 and in use
- this in a storage container which is open to the outside - that is to say to the ambient atmosphere - for example and in particular in the form of a styrofoam container or in a temporarily open by means of a valve, for example and in particular in the form of a closed styrofoam container designed with a pressure relief valve , is present as dry ice, the pressure relief valve opening when a certain pressure is exceeded and then closing again,
- the incubation gas supply is interrupted or made possible by means of a sensor which detects the incubation gas content of the atmosphere of the culture chamber when the incubation gas limit values are exceeded or fallen below, for example and in particular if the inhibition or This is made possible by means of a valve and / or a metering pump.
- the culture chamber has a fan for gas exchange in the culture chamber in order to provide the most homogeneous possible composition in relation to the possible different gaseous components of the atmosphere in the culture chamber.
- the device according to the invention is designed as a transport container, for example and in particular as a case-like container, in order to safely and incubate sensitive cultures even over long distances, for example and in particular by means of an airplane, even across continents to be transported without the cultures to be incubated and transported being damaged during the transport.
- the shock-elastic connection is realized by means of at least one member from the group elastic element, for example and in particular a thermoplastic elastomer, for example and in particular polyisobutadiene, cardanic suspension, spring element.
- the element / part of the device is one from the group incubator, culture chamber, storage container, metering pump, fan, cell culture carrier, cell culture container.
- the C0 2 discharge channel is arranged on an upper third of the storage container in order to only discharge excess gaseous C0 2 from the storage container or the device.
- Figure 2 shows a sketch of several embodiments
- Figure 3 is a sketch of another invention
- FIG. 5 is a sketch of another invention
- Embodiment represents.
- the storage container 4 is a container which is open to the ambient atmosphere, for example and in particular a styrofoam container which has one or more pieces of dry ice T, so that it is constantly and controlled by sublimation C0 2 passes into the gas phase and, if necessary, can be conveyed into the atmosphere of the culture chamber 1 by means of a valve (not shown) and / or for example and in particular a metering pump 6, the culture chamber 1 for example and in particular one or more fans 8 for Has turbulence and thus contribute to the homogenization of the atmosphere of the culture chamber 1.
- FIG. 2 shows the basic principle of the manner in which elements of the device are connected to an inside 11 of the transport container 10 in a shock-elastic manner.
- the incubator 15, the culture chamber 1, the storage container 4 for the C0 2 , the metering pump 6, the fan 5 and the cell culture carrier 16 and the cell culture container 17 can be mounted such that they are connected in a shock-elastic manner by means of elastic elements 12, cardanic suspensions 13 and spring elements 14 from the inside 11 of the transport container 10.
- a gimbal is to be understood to mean hanging at least one of the above-mentioned elements, for example and in particular on an inner side 11 of the transport container 10, for example and in particular with the aid of two intersecting pivot bearings which are mutually perpendicular.
- FIG. 3 shows a sketch of a cell culture carrier 16 which (not shown in more detail) is mounted on a ball bearing in a culture chamber 1 - in addition to a storage container 4 in which dry ice is stored.
- FIG 4 shows a sketch of a cell cooling container 17 resting on a cell culture carrier 16, the cell culture carrier 16 resting on spring elements 14 within a culture chamber 1, the culture chamber 1 itself also having an inner side via an elastic element 12 11 of the transport container 10 is connected.
- FIG. 5 shows a further embodiment of the ⁇ device according to Inventive is sketchy be seen, stored at a storage container 4, in which in use dry ice to an upper third of a C0 2 - is disposed discharge passage 17 in the form of a hose, to remove excess C0 2 can be more or less automatically remove from the inventive device, the required C0 2 for example by means of a valve (not further ge ⁇ shows) for the incubation of a culture chamber 1 supplied.
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Computer Hardware Design (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE102018121781.1A DE102018121781A1 (de) | 2018-09-06 | 2018-09-06 | Vorrichtung zum Inkubieren von Kulturen, Verfahren zum Inkubieren von Kulturen sowie Verwendung |
PCT/DE2019/000234 WO2020048557A1 (de) | 2018-09-06 | 2019-09-06 | Vorrichtung zum inkubieren von kulturen, verfahren zum inkubieren von kulturen sowie verwendung |
Publications (1)
Publication Number | Publication Date |
---|---|
EP3847233A1 true EP3847233A1 (de) | 2021-07-14 |
Family
ID=67847569
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP19779355.7A Pending EP3847233A1 (de) | 2018-09-06 | 2019-09-06 | Vorrichtung zum inkubieren von kulturen, verfahren zum inkubieren von kulturen sowie verwendung |
Country Status (10)
Country | Link |
---|---|
US (1) | US20210348111A1 (de) |
EP (1) | EP3847233A1 (de) |
JP (1) | JP2021534808A (de) |
KR (1) | KR20210055710A (de) |
CN (1) | CN113039261A (de) |
AU (1) | AU2019335850A1 (de) |
CA (1) | CA3111337A1 (de) |
DE (1) | DE102018121781A1 (de) |
IL (1) | IL281131A (de) |
WO (1) | WO2020048557A1 (de) |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
NZ207360A (en) * | 1983-03-23 | 1987-06-30 | Hickinbotham Winemakers | Method and apparatus for producing wine by full carbonic maceration |
WO2005093038A1 (de) * | 2004-03-25 | 2005-10-06 | Paul Dietl | Inkubator |
JP4916201B2 (ja) * | 2006-03-27 | 2012-04-11 | 三洋電機株式会社 | 培養装置 |
DE102010012790B4 (de) * | 2010-03-25 | 2015-08-06 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Inkubatoreinrichtung und Verfahren zu deren Betrieb |
DE102014106877B4 (de) * | 2014-05-15 | 2016-03-24 | Ert Optik Dr. Thiel Gmbh | Modulares Inkubatorsystem |
CN206666559U (zh) * | 2017-04-07 | 2017-11-24 | 阜阳师范学院 | 便携式自带co2培养箱 |
-
2018
- 2018-09-06 DE DE102018121781.1A patent/DE102018121781A1/de active Pending
-
2019
- 2019-09-06 CA CA3111337A patent/CA3111337A1/en active Pending
- 2019-09-06 JP JP2021512577A patent/JP2021534808A/ja active Pending
- 2019-09-06 AU AU2019335850A patent/AU2019335850A1/en active Pending
- 2019-09-06 KR KR1020217008131A patent/KR20210055710A/ko unknown
- 2019-09-06 CN CN201980058365.0A patent/CN113039261A/zh active Pending
- 2019-09-06 WO PCT/DE2019/000234 patent/WO2020048557A1/de active Application Filing
- 2019-09-06 EP EP19779355.7A patent/EP3847233A1/de active Pending
- 2019-09-06 US US17/273,854 patent/US20210348111A1/en active Pending
-
2021
- 2021-02-25 IL IL281131A patent/IL281131A/en unknown
Also Published As
Publication number | Publication date |
---|---|
JP2021534808A (ja) | 2021-12-16 |
CN113039261A (zh) | 2021-06-25 |
AU2019335850A1 (en) | 2021-03-25 |
KR20210055710A (ko) | 2021-05-17 |
CA3111337A1 (en) | 2020-03-12 |
US20210348111A1 (en) | 2021-11-11 |
IL281131A (en) | 2021-04-29 |
WO2020048557A1 (de) | 2020-03-12 |
DE102018121781A1 (de) | 2020-03-12 |
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Owner name: CELLBOX SOLUTIONS GMBH |
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