EP3763812A4 - ARGONAUT PROTEIN MUTANT AND CORRESPONDING USE - Google Patents

ARGONAUT PROTEIN MUTANT AND CORRESPONDING USE Download PDF

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Publication number
EP3763812A4
EP3763812A4 EP19763666.5A EP19763666A EP3763812A4 EP 3763812 A4 EP3763812 A4 EP 3763812A4 EP 19763666 A EP19763666 A EP 19763666A EP 3763812 A4 EP3763812 A4 EP 3763812A4
Authority
EP
European Patent Office
Prior art keywords
protein mutant
argonaute protein
argonaute
mutant
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP19763666.5A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP3763812A1 (en
Inventor
Jianguang Zhang
Aiping MAO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Berry Genomics Co Ltd
Original Assignee
Berry Genomics Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Berry Genomics Co Ltd filed Critical Berry Genomics Co Ltd
Priority claimed from PCT/CN2019/070253 external-priority patent/WO2019169945A1/zh
Publication of EP3763812A1 publication Critical patent/EP3763812A1/en
Publication of EP3763812A4 publication Critical patent/EP3763812A4/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/85—Fusion polypeptide containing an RNA binding domain

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP19763666.5A 2018-03-06 2019-01-03 ARGONAUT PROTEIN MUTANT AND CORRESPONDING USE Pending EP3763812A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201810184689 2018-03-06
CN201811505553.7A CN110229799B (zh) 2018-03-06 2018-12-10 Argonaute蛋白突变体及其用途
PCT/CN2019/070253 WO2019169945A1 (zh) 2018-03-06 2019-01-03 Argonaute蛋白突变体及其用途

Publications (2)

Publication Number Publication Date
EP3763812A1 EP3763812A1 (en) 2021-01-13
EP3763812A4 true EP3763812A4 (en) 2021-11-17

Family

ID=67861926

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19763666.5A Pending EP3763812A4 (en) 2018-03-06 2019-01-03 ARGONAUT PROTEIN MUTANT AND CORRESPONDING USE

Country Status (3)

Country Link
US (1) US20210332353A1 (zh)
EP (1) EP3763812A4 (zh)
CN (1) CN110229799B (zh)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112538470B (zh) * 2020-12-11 2023-03-28 湖北大学 一种原核生物来源的Argonaute蛋白及其应用
CN113201578B (zh) * 2021-04-29 2023-06-23 上海交通大学 一种新型高温Argonaute蛋白TpsAgo表征及应用
CN113373129B (zh) * 2021-05-25 2022-07-26 湖北大学 一种原核生物来源的Mbp_Argonaute蛋白及其应用
CN114606215B (zh) * 2022-01-24 2022-11-29 湖北大学 一种真核生物来源的Argonaute蛋白及其应用
WO2023150632A2 (en) * 2022-02-02 2023-08-10 The Broad Institute, Inc. Targeting moieties promoting transduction of central nervous system cells and tissues and methods of use
CN114634968B (zh) * 2022-02-28 2024-05-31 复旦大学 基于Argonaute蛋白的场效应晶体管核酸传感器及其制备方法和应用
CN114561374A (zh) * 2022-03-11 2022-05-31 上海交通大学 一种新型嗜热核酸内切酶突变体及其制备方法和应用
CN115927251B (zh) * 2022-07-07 2023-10-20 湖北大学 一种仅具有RNA靶切割活性的原核Argonaute蛋白质在RNA编辑中的应用
CN116656648B (zh) * 2023-05-16 2024-02-23 湖北大学 具有中温靶核酸切割活性的PfAgo突变体蛋白及其应用
CN117778377A (zh) * 2023-12-14 2024-03-29 湖北大学 基于新型可编程核酸酶Argonaute的大片段DNA高效合成与组装方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015157534A1 (en) * 2014-04-10 2015-10-15 The Regents Of The University Of California Methods and compositions for using argonaute to modify a single stranded target nucleic acid
WO2018011236A1 (en) * 2016-07-12 2018-01-18 Wageningen Universiteit Prokaryotic argonaute proteins and uses thereof

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006015258A2 (en) * 2004-07-28 2006-02-09 Cold Spring Harbor Laboratory Methods and compositions related to argonaute proteins

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015157534A1 (en) * 2014-04-10 2015-10-15 The Regents Of The University Of California Methods and compositions for using argonaute to modify a single stranded target nucleic acid
WO2018011236A1 (en) * 2016-07-12 2018-01-18 Wageningen Universiteit Prokaryotic argonaute proteins and uses thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
OLOVNIKOV IVAN ET AL: "Bacterial Argonaute Samples the Transcriptome to Identify Foreign DNA", MOLECULAR CELL, vol. 51, no. 5, 12 September 2013 (2013-09-12), pages 594 - 605, XP028716262, ISSN: 1097-2765, DOI: 10.1016/J.MOLCEL.2013.08.014 *
See also references of WO2019169945A1 *

Also Published As

Publication number Publication date
EP3763812A1 (en) 2021-01-13
CN110229799B (zh) 2023-06-23
CN110229799A (zh) 2019-09-13
US20210332353A1 (en) 2021-10-28

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