EP3728620A4 - Genstellensättigungsmutagenese(gssm)-verfahren - Google Patents
Genstellensättigungsmutagenese(gssm)-verfahren Download PDFInfo
- Publication number
- EP3728620A4 EP3728620A4 EP18891128.3A EP18891128A EP3728620A4 EP 3728620 A4 EP3728620 A4 EP 3728620A4 EP 18891128 A EP18891128 A EP 18891128A EP 3728620 A4 EP3728620 A4 EP 3728620A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- gssm
- saturation mutagenesis
- gene site
- site saturation
- gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/101—Plasmid DNA for bacteria
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201762608167P | 2017-12-20 | 2017-12-20 | |
PCT/US2018/066294 WO2019126211A1 (en) | 2017-12-20 | 2018-12-18 | Gene site saturation mutagenesis (gssm) method |
Publications (2)
Publication Number | Publication Date |
---|---|
EP3728620A1 EP3728620A1 (de) | 2020-10-28 |
EP3728620A4 true EP3728620A4 (de) | 2021-11-03 |
Family
ID=66993889
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP18891128.3A Pending EP3728620A4 (de) | 2017-12-20 | 2018-12-18 | Genstellensättigungsmutagenese(gssm)-verfahren |
Country Status (5)
Country | Link |
---|---|
US (1) | US20220251540A1 (de) |
EP (1) | EP3728620A4 (de) |
CN (1) | CN111492063A (de) |
CA (1) | CA3083527A1 (de) |
WO (1) | WO2019126211A1 (de) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002016606A2 (fr) * | 2000-08-25 | 2002-02-28 | Biomethodes | Procede de mutagenese dirigee massive |
WO2009018449A1 (en) * | 2007-07-31 | 2009-02-05 | Verenium Corporation | Tailored multi-site combinatorial assembly |
WO2009088933A1 (en) * | 2007-12-31 | 2009-07-16 | Xoma Technology Ltd. | Methods and materials for targeted mutagenesis |
US20090312196A1 (en) * | 2008-06-13 | 2009-12-17 | Codexis, Inc. | Method of synthesizing polynucleotide variants |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6171820B1 (en) * | 1995-12-07 | 2001-01-09 | Diversa Corporation | Saturation mutagenesis in directed evolution |
US20090130718A1 (en) * | 1999-02-04 | 2009-05-21 | Diversa Corporation | Gene site saturation mutagenesis |
HUE041367T2 (hu) * | 2008-06-13 | 2019-05-28 | Codexis Inc | Eljárás polinukleotid-változatok szintézisére |
WO2014189287A1 (en) * | 2013-05-21 | 2014-11-27 | Industry And Academia Cooperation Foundation, Myongji University | Primers and kits for colony multiplex pcr for the detection of class a, b, c, and d b-lactamase genes and methods of using thereof |
-
2018
- 2018-12-18 CA CA3083527A patent/CA3083527A1/en active Pending
- 2018-12-18 WO PCT/US2018/066294 patent/WO2019126211A1/en unknown
- 2018-12-18 CN CN201880081803.0A patent/CN111492063A/zh active Pending
- 2018-12-18 EP EP18891128.3A patent/EP3728620A4/de active Pending
- 2018-12-18 US US16/954,092 patent/US20220251540A1/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002016606A2 (fr) * | 2000-08-25 | 2002-02-28 | Biomethodes | Procede de mutagenese dirigee massive |
WO2009018449A1 (en) * | 2007-07-31 | 2009-02-05 | Verenium Corporation | Tailored multi-site combinatorial assembly |
WO2009088933A1 (en) * | 2007-12-31 | 2009-07-16 | Xoma Technology Ltd. | Methods and materials for targeted mutagenesis |
US20090312196A1 (en) * | 2008-06-13 | 2009-12-17 | Codexis, Inc. | Method of synthesizing polynucleotide variants |
Non-Patent Citations (2)
Title |
---|
JONES BRYAN J. ET AL: "Comparison of Five Protein Engineering Strategies for Stabilizing an [alpha]/[beta]-Hydrolase", BIOCHEMISTRY, vol. 56, no. 50, 19 December 2017 (2017-12-19), pages 6521 - 6532, XP055842421, ISSN: 0006-2960, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736438/pdf/nihms916946.pdf> DOI: 10.1021/acs.biochem.7b00571 * |
See also references of WO2019126211A1 * |
Also Published As
Publication number | Publication date |
---|---|
CN111492063A (zh) | 2020-08-04 |
WO2019126211A1 (en) | 2019-06-27 |
US20220251540A1 (en) | 2022-08-11 |
EP3728620A1 (de) | 2020-10-28 |
CA3083527A1 (en) | 2019-06-27 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE |
|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE |
|
17P | Request for examination filed |
Effective date: 20200720 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
|
AX | Request for extension of the european patent |
Extension state: BA ME |
|
DAV | Request for validation of the european patent (deleted) | ||
DAX | Request for extension of the european patent (deleted) | ||
A4 | Supplementary search report drawn up and despatched |
Effective date: 20211005 |
|
RIC1 | Information provided on ipc code assigned before grant |
Ipc: C12Q 1/68 20180101ALI20210929BHEP Ipc: C12N 15/10 20060101ALI20210929BHEP Ipc: C12P 19/34 20060101AFI20210929BHEP |