EP3728620A4 - Genstellensättigungsmutagenese(gssm)-verfahren - Google Patents

Genstellensättigungsmutagenese(gssm)-verfahren Download PDF

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Publication number
EP3728620A4
EP3728620A4 EP18891128.3A EP18891128A EP3728620A4 EP 3728620 A4 EP3728620 A4 EP 3728620A4 EP 18891128 A EP18891128 A EP 18891128A EP 3728620 A4 EP3728620 A4 EP 3728620A4
Authority
EP
European Patent Office
Prior art keywords
gssm
saturation mutagenesis
gene site
site saturation
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP18891128.3A
Other languages
English (en)
French (fr)
Other versions
EP3728620A1 (de
Inventor
Jared DENNIS
Robin CAI
Xuqiu Tan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BASF SE
Original Assignee
BASF SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BASF SE filed Critical BASF SE
Publication of EP3728620A1 publication Critical patent/EP3728620A1/de
Publication of EP3728620A4 publication Critical patent/EP3728620A4/de
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/101—Plasmid DNA for bacteria

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
EP18891128.3A 2017-12-20 2018-12-18 Genstellensättigungsmutagenese(gssm)-verfahren Pending EP3728620A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762608167P 2017-12-20 2017-12-20
PCT/US2018/066294 WO2019126211A1 (en) 2017-12-20 2018-12-18 Gene site saturation mutagenesis (gssm) method

Publications (2)

Publication Number Publication Date
EP3728620A1 EP3728620A1 (de) 2020-10-28
EP3728620A4 true EP3728620A4 (de) 2021-11-03

Family

ID=66993889

Family Applications (1)

Application Number Title Priority Date Filing Date
EP18891128.3A Pending EP3728620A4 (de) 2017-12-20 2018-12-18 Genstellensättigungsmutagenese(gssm)-verfahren

Country Status (5)

Country Link
US (1) US20220251540A1 (de)
EP (1) EP3728620A4 (de)
CN (1) CN111492063A (de)
CA (1) CA3083527A1 (de)
WO (1) WO2019126211A1 (de)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002016606A2 (fr) * 2000-08-25 2002-02-28 Biomethodes Procede de mutagenese dirigee massive
WO2009018449A1 (en) * 2007-07-31 2009-02-05 Verenium Corporation Tailored multi-site combinatorial assembly
WO2009088933A1 (en) * 2007-12-31 2009-07-16 Xoma Technology Ltd. Methods and materials for targeted mutagenesis
US20090312196A1 (en) * 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6171820B1 (en) * 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US20090130718A1 (en) * 1999-02-04 2009-05-21 Diversa Corporation Gene site saturation mutagenesis
HUE041367T2 (hu) * 2008-06-13 2019-05-28 Codexis Inc Eljárás polinukleotid-változatok szintézisére
WO2014189287A1 (en) * 2013-05-21 2014-11-27 Industry And Academia Cooperation Foundation, Myongji University Primers and kits for colony multiplex pcr for the detection of class a, b, c, and d b-lactamase genes and methods of using thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002016606A2 (fr) * 2000-08-25 2002-02-28 Biomethodes Procede de mutagenese dirigee massive
WO2009018449A1 (en) * 2007-07-31 2009-02-05 Verenium Corporation Tailored multi-site combinatorial assembly
WO2009088933A1 (en) * 2007-12-31 2009-07-16 Xoma Technology Ltd. Methods and materials for targeted mutagenesis
US20090312196A1 (en) * 2008-06-13 2009-12-17 Codexis, Inc. Method of synthesizing polynucleotide variants

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JONES BRYAN J. ET AL: "Comparison of Five Protein Engineering Strategies for Stabilizing an [alpha]/[beta]-Hydrolase", BIOCHEMISTRY, vol. 56, no. 50, 19 December 2017 (2017-12-19), pages 6521 - 6532, XP055842421, ISSN: 0006-2960, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736438/pdf/nihms916946.pdf> DOI: 10.1021/acs.biochem.7b00571 *
See also references of WO2019126211A1 *

Also Published As

Publication number Publication date
CN111492063A (zh) 2020-08-04
WO2019126211A1 (en) 2019-06-27
US20220251540A1 (en) 2022-08-11
EP3728620A1 (de) 2020-10-28
CA3083527A1 (en) 2019-06-27

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