EP3675885A1 - Composition for improving the well-being of animals - Google Patents
Composition for improving the well-being of animalsInfo
- Publication number
- EP3675885A1 EP3675885A1 EP18807884.4A EP18807884A EP3675885A1 EP 3675885 A1 EP3675885 A1 EP 3675885A1 EP 18807884 A EP18807884 A EP 18807884A EP 3675885 A1 EP3675885 A1 EP 3675885A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- composition
- strain
- cncm
- terrestrial
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/007—Pulmonary tract; Aromatherapy
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K2035/11—Medicinal preparations comprising living procariotic cells
- A61K2035/115—Probiotics
Definitions
- the invention relates to a composition for improving the welfare of animals, particularly farm animals.
- Sanitary problems in farms are often the consequence of the combination of several pathogens. These can be present in different microbial ecosystems: the environment (air, organic or inorganic surfaces), in the internal system of the animal (digestive or respiratory system) or on the animal (cutaneous system).
- Antibiotic treatment strategies have been used for a very long time but pose two major problems: on the one hand, the resistance generated by the too regular use of antibiotics, and on the other hand, a public health problem by the consumption of chairs. including many antibiotics.
- the object of the invention is to overcome the aforementioned drawbacks.
- Another object of the invention is to provide a new and effective method for maintaining the health and welfare of terrestrial livestock.
- Another object of the invention is to provide a kit that can be used directly by breeders.
- the invention relates to a bacterial composition
- a bacterial composition comprising at least one strain of bacteria of the genus Bacillus , in particular Bacillus subtilis and at least one strain of lactic bacteria, in particular at least one strain of Lactoco c cus lactis , for use in the prevention or treatment of pathogenic infections affecting the health of terrestrial livestock,
- composition being:
- the invention is based on the surprising finding made by the inventors that a mixture of at least one strain of bacteria of the genus Bacillus subtilis and at least one strain of the genus Lactococcus lactis , non-pathogenic bacteria, act synergistically on the animal health of terrestrial animals when said mixture is both ingested or inhaled by the animals and is spread in the culture medium, or applied directly to the animal, or both .
- the spreading is assimilated to a dispersion; both of these terms can be used uniformly in the invention.
- the inventors have demonstrated a synergistic protective effect against pathogenic when bacteria described herein (Bacillus and Lactoco ECU c) are both present in the gastrointestinal or respiratory tract and in the rearing environment and / or on the animal.
- bacterial strain is understood to mean a group of individuals from successive subcultures of a bacterial colony.
- a bacterial strain is a part of a bacterial species different from other bacteria of the same species by a minor but identifiable difference.
- pathogenic infections means infections by the following microorganisms: Salmonella, Staphylococcus, Streptococci, Clostridium, Listeria, Arcanobacterium, Mycoplasma, Klebsielle, Pasteurella, Bordetella, Campylobacter, Brachyspire, Escherichia coli and / or responsible for pathologies enteritis, salmonellosis, ulcers, infectious lameness, rhinitis, mastitis, epidermitis, necrosis, pasteurellosis, bordetellosis, listeriosis.
- the aforementioned composition comprises at least two strains of Bacillus , and in particular at least two different strains of Bacillus. and at least one strain of lactic acid bacterium, in particular L actococcus lactis .
- the aforementioned composition comprises at least three strains of Bacillus , and in particular at least three different strains of Bacillus , and at least one strain of lactic acid bacterium, especially L actococcus lactis .
- the aforementioned composition comprises at least four strains of Bacillus , and in particular at least four different strains of Bacillus. and at least one strain of lactic acid bacterium, in particular L actococcus lactis .
- an application in the environment with reference to the spreading in the environment, of an external application on the animal (A) with reference to the application on the animal, and an internal application (I), with reference to ingestion or inhalation.
- the application E makes it possible to control and / or guide the bacterial flora of the environment of terrestrial livestock by reducing the pathogenic pressure. It can be done in nebulization or spraying of livestock buildings, incorporation into litter ... There is less contamination of animals because there are fewer pathogenic foci. Such an application plays on living or surviving pathogens in the environment.
- the modes of spreading in the environment are the spraying, the nebulization, the covering of surfaces by foams or the dusting of powder.
- Spreading is generally carried out on the surfaces of the farm, mainly on surfaces in direct or potential contact with the animals, preferably on cleaned and disinfected surfaces, after waiting for the end of the remanence of the disinfectants.
- Spreading in the environment is carried out at least once after a crawl space, after a protocol of cleaning / disinfection or before arrival of the animals. It is particularly advantageous to practice a repeated application every week.
- Application A makes it possible to control or orient the microbism of the animal by reducing the pathogenic pressure.
- the application can be done by nebulization, spraying, soaking of the terrestrial animal. This generates less contamination of the animals because there are fewer pathogenic foci directly on the animal.
- the application to the animal can be done by spraying, nebulization, soaking, footbath, foam, depending on the area of the animal on which it is desired to deposit the composition according to the invention.
- composition at least once to the animal as early as possible in the life of the animal or upon arrival in a new environment.
- the application is repeated every week.
- Application I makes it possible to control and / or guide digestive microbism by decreasing the pathogenic pressure and / or by stimulating the animals' natural defenses.
- the application can be via the drinking water, the diet or any means allowing the animal to ingest the composition of the invention.
- the composition according to the invention directly affects the digestive health of terrestrial livestock, which limits the entry of other pathogens.
- Application I may also be an application by inhalation, nebulization or spraying in the respiratory tract.
- Application I should be started as soon as possible, before the start of the health risk phase and for the duration of the health risk period. It is recommended to renew the application at least every 10 days, at best every day.
- terrestrial livestock means all the animals are not aquatic, and can be used directly or indirectly for human or animal nutrition. This concerns in particular cattle, sheep, pigs, goats, rabbits, equines, insects but also farmed birds such as chickens, ducks, geese, turkeys, quails and other guinea-pigs. 'breeding.
- the invention relates to a composition for its aforementioned use, wherein the bacterial composition comprises:
- Bacillus subtilis strains NOL01, NOL02, NOL03, said strains being respectively deposited at the CNCM under the number CNCM I-4606, CNCM I-5043 and CNCM I-4607, and
- lactic acid bacterium strain Lactococcus lactis spp lactis 1 strain NOL11, said strain being deposited at the CNCM under number CNCM I-4609.
- the invention relates to the use of at least one strain chosen from the NOL01, NOL02 and NOL03 strains and at least the NOL11 strain. Also, the invention covers the following 7 combinations:
- the invention concerns a composition for the aforementioned use, said composition comprising 10 April to 10 November bacterial colonies of Bacillus and April 10 to November 10 bacterial colonies of lactic acid bacteria, bacterial colonies being in grams or milliliter of composition.
- composition according to the invention if the composition according to the invention is in liquid form, said composition will comprise 10 April to 10 November Bacillus bacterial colonies per ml of composition, and this for each of the strains when the composition comprises at least two strains and April 10 to November 10 bacterial colonies of lactic acid bacteria per ml of composition.
- composition of the invention If, against the composition of the invention is dried or non-aqueous, said composition will comprise 10 April to 10 November Bacillus bacterial colonies per gram of composition, and this for each of the strains when the composition comprises at least two strains and 10 4 to 10 11 bacterial colonies of lactic acid bacteria per gram of composition.
- from 4 to 10 11 bacterial colonies means: about 10 4 , about 5 ⁇ 10 4 , about 10 5 , about 5 ⁇ 10 5 , about 10 6 , about 5 ⁇ 10 6 , about 10 7 , about 5.10 7 , about 10 8 , about 5 ⁇ 10 8 , about 10 9 , about 5 ⁇ 10 9 , about 10 10 , about 5 ⁇ 10 or about 10 11 bacterial colonies.
- Bacterial colonies are per mL (or gram) of bacterial culture. Those skilled in the art will readily know how to determine this number of bacteria, in particular by counting either manually (using a Malassez slide) or by using an automatic cell counter, or by dilution and then agar seeding and colony counting.
- the invention relates to a composition for its use as defined above, wherein said at least one strain of Bacillus is in sporulated form and / or in vegetative form.
- Lactic acid bacteria are always in vegetative form.
- the composition according to the invention comprises at least one strain of Bacillus spore form and at least one strain of lactic acid bacterium in vegetative form, or comprises at least one strain of Bacillus in vegetative form and at least one strain of lactic acid bacterium in form vegetative.
- the invention relates to a composition for its use defined above, wherein said inhaled or ingested composition and said composition spread in the medium for rearing said terrestrial livestock and / or applied to said farm animals terrestrial, are used simultaneously, separately or spread over time.
- the invention relates to a composition for its aforementioned use, wherein said composition is spread and / or applied prior to ingestion or inhalation by said terrestrial livestock.
- the spreading in the environment is done according to the doses as defined above.
- the invention relates to a composition for its aforementioned use, wherein said composition consists essentially of the four bacterial strains of the group consisting of the three strains NOL01, NOL02, NOL03, said strains being respectively deposited at the CNCM under number CNCM I - 4606, CNCM I-5043 and CNCM I - 4607 and the Lactococcus strain lactis spp lactis 1 strain NOL11, said strain being deposited at the CNCM under number CNCM I - 4609.
- composition comprises the above-mentioned three strains of Bacillus subtilis bacteria, in vegetative or sporulated form, and the above-mentioned lactic acid bacteria strain, in vegetative form.
- the invention relates to a composition for its use as defined above, wherein said composition is spread by m 2 in the proportion of 10 5 to 11 bacterial colonies.
- composition in the context of the application in the environment of farm animals, it is advantageous for the composition to be dispersed or spread at a rate of 10 5 to 10 11 bacterial colonies for a dry or dehydrated composition, or 10 5 to 10 11 bacterial colonies for a liquid composition per m 2 of surface or medium to be treated.
- the invention relates to a composition for its use mentioned above, where the environment is cleaned and disinfected and the terrestrial animals are washed prior to the application in the breeding environment of said terrestrial livestock and / or the application on said terrestrial livestock.
- composition according to the invention It may be advantageous to clean the environment and / or the animals before using the composition according to the invention. This cleaning can be assimilated to a disinfection. Before implantation of the flora of the composition according to the invention, if a cleaning or disinfection is operated, the better the effectiveness of the bacteria, and the better the benefit for the animals. Surfaces may be treated by any cleaning means known to those skilled in the art. Regarding the cleaning of animals, it is possible to wash, bathe or even shampoo.
- the invention further provides a kit for terrestrial livestock against pathogenic infections affecting the health and welfare of farm animals including
- biosafety agent means a chemical or physical agent capable of reducing the number or inactivating microorganisms and their biofilms. These include, for example, disinfectants, detergents, UV radiation, or steam or any other biocidal agent. This list is not exhaustive, and the person skilled in the art knows the biosecurity agents.
- biocontrol agent is intended to mean any composition of living organisms or organisms derived from living organisms, or a composition of natural substances intended to protect animals from a health point of view (macro-organisms (insects, nematodes, etc.), micro-organisms (viruses, bacteria, phages or fungi), chemical mediators (pheromones), or natural substances of mineral, vegetable or animal origin, such as, for example, essential oils).
- kits which comprises the composition as defined above, and in particular a composition comprising the bacteria NOL01, NOL02, and NOL03 with the bacterium NOL11, and in parallel at least one biocontrol agent. and / or biosecurity.
- This kit allows the user to have the means to apply the composition according to the invention, and if necessary, beforehand, or after the treatment with the composition according to the invention, to use one or more other agents such as as defined above.
- the aforementioned agent should not be used simultaneously with the composition according to the invention if it has an antibacterial effect.
- ICC corrected consumption index
- ICC corrected consumption index
- a box with synthetic mustache indicates the values of means, medians, quantiles and indices of confidence for all the animals resulting from the batches tests (left) or witnesses (right).
- the abscissa differentiates control (light gray on the left) and test (dark gray on the right) batches.
- the ordinate represents the percentage of tarsi burned.
- the graphs show the median value of the two lots and their confidence interval.
- the abscissa differentiates control (light gray on the left) and test (dark gray on the right) batches.
- the ordinate represents the percentage of tarsi burned.
- the graphs show the median value of the two lots and their confidence interval.
- the abscissa differentiates control (light gray on the left) and test (dark gray on the right) batches.
- the ordinate represents the antibiotic consumption value.
- the graphs show the median value for both lots and their confidence interval.
- the abscissa differentiates treatments performed for problems related to lameness (A.) or treatments performed in the first 10 days of life of animals (start B.).
- the ordinate represents the number of days of antibiotic treatment.
- the results for test lots are differentiated in light gray and the control groups are dark gray.
- the abscissa differentiates control (light gray on the left) and test (dark gray on the right) batches.
- the ordinate represents the average weight of the animals.
- the graphs show the median value of the two lots and their confidence interval.
- the abscissa differentiates control (light gray on the left) and test (dark gray on the right) batches.
- the ordinate represents the average weight of the animals.
- the graphs show the median value of the two lots and their confidence interval.
- the x-axis represents the days of life of the piglets compared to the date of birth (0).
- the ordinate represents the probability that at least one piglet in one cell has diarrhea.
- the three grouped curves above represent the modelizations of the probabilities of diarrhea for lots 1 to 3 and the isolated curve below for lot 4.
- the x-axis represents the days of life of the piglets compared to the date of birth (0).
- the ordinate represents the probability that a piglet dies.
- the three grouped curves above represent the modelizations of the probabilities of diarrhea for lots 1 to 3 and the isolated curve below for lot 4.
- the inventors seek to show whether an application of the composition according to the invention in the environment or on the litter, alone, to an interest for the prevention of pathogenic infections.
- the tested product is an association of living bacteria (strains of Bacillus subtilis and Lactococcus lactis ) in their culture medium, brought frozen at the farm, to be diluted in water before spraying.
- the test is performed in the calf building with a control lot and a lot where the product will be sprayed in the environment and on the litter. The impact of the product will be studied over time.
- the influence of the product will be measured on the chemical qualities of the litter, the cleanliness of the animals and the sanitary balance of the batch (frequency and nature of pathologies, veterinary costs) over time.
- a dose of composition is packaged in two separate bottles of 20 ml:
- Bottle B containing bacillus (NOL01, NOL02, NOL03)
- the flasks are brought frozen in breeding.
- Water used during spraying must not contain chlorine. Pre-treatment of water with sodium thiosulfate may therefore be necessary.
- the material needed for processing and measurements is as follows:
- the weight of the calves is estimated at the beginning of the test using a bovimetric tape.
- the distribution of calves in each box was carried out to obtain a comparable total live weight between the control and test boxes.
- the test lasted 2 months, period between two cleaning sessions.
- the first application of the product is carried out after the cleaning in week 16.
- the first measurements are made just before the first application of flora. Then the measurements are made every week before application of the product. End of the applications of the product in week 23 and last measurement just before the second cleaning in week 24.
- 1 L of diluted composition is used to treat the 30 m2 calf test.
- the breeder keeps his usual mulching practice for the duration of the test.
- the objective is to determine if the application on the animal can have a better effect.
- the objective of this trial is to test the application of the barrier flora (frozen combination of Bacillus and Lactococcus ) from the hatchery, on chicks at D0, in order to study its impact on the sanitary quality of the batches.
- the test will be conducted on chicks for 20 farms, which will be compared to contemporary control batches. In each farm under test, only the chicks of a building will be treated in hatchery, the chicks of the second building will have undergone no treatment (witness).
- the aim is to verify an effect on the decrease of mortality, health improvement, improvement of zootechnical indices.
- composition according to the invention is applied (strains of Bacillus subtilis NOL01, NOL02, NOL03 and Lactococcus lactis NOL03) directly on the chicks after sexing.
- the doses will be thawed in a 27 ° C water bath for 9 minutes.
- the studied parameters are: food and water consumption, mortality and elimination as well as slaughterhouse data (technical and economic results, seizures, enumeration of pododermatitis, tarsi, infected skin lesions or LCI and keel bone).
- the objective of this test is to test the effectiveness of the composition according to the invention (NOL01, NIL02, NOL03, NOL11) both on the animal and in the environment.
- the aim is to identify an optimal use for the prevention of pathologies (lameness, colibacillosis, etc.) and / or intestinal health and the improvement of litter quality and therefore of animal welfare.
- the first breeding tested has 2 comparable buildings, with litter buckwheat husks on concrete floor. It is equipped with load cells. Its drinking water is treated with 3% 80% pure hypochlorous acid (electrolytic treatment system - DCW company).
- the second breeding has 2 comparable buildings, with litter buckwheat husks and wood chips on unpaved soil. It is equipped with load cells. Its drinking water is treated with hydrogen peroxide, stop treatment when spraying the flora.
- the influence of the product is measured on the growth performance, the sanitary balance of the batch (frequency and nature of the pathologies, veterinary costs), the bacteriological counts (litter), the gas emissions, the quality of the litters and the criteria of good -be.
- Seeding at D0 is performed after placement.
- test and control buildings are distinguished only by the application or not of barrier flora.
- the product is sprayed by misting (approximately 15-20L of water containing the flora at each application, for 1200 m2).
- the water used is untreated artesian well water.
- the purpose of this test is to determine whether a combination of the applications of the composition described in Example 1 in the environment, on the animal and ingestion has an efficacy on the prevention of pathogenic infections.
- a preliminary statistical study made it possible to classify poultry flocks according to the proximity of the zootechnical results of two livestock buildings. This classification includes all farms with at least 5 bands since the beginning of 2014 for which the weight, mortality, ICC and pododermatite values have been completed. This study established a list of 60 farms with the closest results between two buildings.
- test will therefore take place in about twenty farms on this list with each test farm, a control building and a test building receiving an application of barrier flora. Before the integration of each farm in the test, it will be ensured that the two buildings under test will receive the same lines of the same hatchery during 2 bands.
- the application of the product will be done with battery sprayers or with the sprayers of the breeders used for the vaccination (after validation of the material: sufficient capacity, practicality, not of use for disinfection treatments or any other incompatible product).
- the material needed for processing and measurements is as follows:
- Figures 4 to 10 show the results obtained for lameness, mortality, treatment with antibiotics, tarsi burned in males, and food consumption.
- Figures 4 and 5 show a significant decrease in the number of animals having tarsi burned in males ( Figure 4 ) or females ( Figure 5 ) during the triple treatment with the composition according to the invention, compared to the control sample .
- Figures 6 and 7 show that animals treated with the composition according to the invention according to the three modes of application significantly less need antibiotics. There was a trend of 12 ppm / kg live weight of antibiotic administered less in the test lots compared to controls, a 39% decrease in antibiotic consumption.
- This test aims to show whether a combined application of the composition described in Example 1 in the environment, on the animal and by inhalation is effective. So check against the previous example, if other types of applications can be made such as inhalation instead of ingestion.
- animals treated with the composition according to the invention are compared according to the three treatment modes with respect to animals that have not received the composition of the invention (placebo).
- the application was carried out with a nebulizer in the presence of the animals so the flora was applied to the surfaces of the rooms (E), animals (A) and inhalation (I).
- Pigs randomly taken from Livestock 2 were analyzed after slaughter by measuring deformities on slit sections.
- the placebo sample indicates 30% of the individuals with a very good anatomical nasal state (absence of deviation of the nasal septum, absence of atrophy of the scrolls and nasal turbinates).
- test sample (pigs bred in the presence of barrier flora from post-weaning to the end of breeding) reveals that twice as many (63%) of individuals have a very good anatomic state of the nasal vs placebo .
- This test also shows the importance of the application environment whose efficiency is improved when the cleaning and disinfection of the surfaces upstream of the application is well done.
- the interest of the product is measured both on the animals (sanitary), and vis-à-vis the breeder (working conditions).
- EXAMPLE 6 Application in the environment and on the animal in comparison with application environment, animal and ingested: pigs
- the breeding followed is a breeding of 700 sows fattener breeder selection (5 strips of 120 sows). The trial was carried out on 2 bands in which 3 batches of 40 sows were formed, with a uniform distribution of the litter ranks.
- the application protocol includes spraying a barrier flora into the environment, on the udders and perineum of sows, and probiotic administration to sows via the soup machine.
- Figures 11 and 12 show that with the three applications combined the risk of developing diarrhea is less important and the risk of mortality also compared to an environment-only and animal-only application.
- Figure 13 shows that more boxes with piglets whose mothers received a shampoo before the application of flora are healthy from a digestive point of view compared to the boxes of piglets whose sows have not received shampoo . Indeed, in the first case (band 2) one observes at the lowest 78 cases out of 120 with normal feces whereas in the second case (band 1), only 50 boxes out of 120.
- the inventors have shown that the application A + E + I is more effective than the application A + E.
- they have shown that if we promote the establishment of flora on the animal (A better application), we improve the prevention of diarrhea.
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Abstract
Description
- L’invention concerne une composition visant à améliorer le bien-être des animaux, en particulier les animaux d’élevage.The invention relates to a composition for improving the welfare of animals, particularly farm animals.
- Les problèmes sanitaires en élevages sont souvent la conséquence de la combinaison de plusieurs pathogènes. Ceux-ci peuvent être présents dans différents écosystèmes microbiens : l’environnement (air, surfaces organiques ou inorganiques), dans le système interne de l’animal (système digestif ou respiratoire) ou sur l’animal (appareil cutané).Sanitary problems in farms are often the consequence of the combination of several pathogens. These can be present in different microbial ecosystems: the environment (air, organic or inorganic surfaces), in the internal system of the animal (digestive or respiratory system) or on the animal (cutaneous system).
- Dans les différents types d’élevages d’animaux terrestres, on retrouve toujours des phases à risque où les écosystèmes microbiens évoluent fortement, comme par exemple une phase de réimplantation d’une flore externe après désinfection, l’arrivée d’animaux dans un élevage, les premiers instants de vie des nouveaux nés, l’arrivée de nouveaux individus dans un lot d’animaux déjà existants, les transitions alimentaires, la phase de réimplantation d’une flore interne après utilisation d’antibiotique etc…In the different types of terrestrial animal breeding, we always find risk phases where microbial ecosystems are changing strongly, for example a phase of reimplantation of an external flora after disinfection, the arrival of animals in a farm , the first moments of life of newborns, the arrival of new individuals in a lot of already existing animals, food transitions, the phase of reimplantation of an internal flora after use of antibiotic etc ...
- Aussi, est-il nécessaire de contrôler cet environnement afin de maintenir l’état sanitaire des élevages et préserver le bien-être des animaux.Therefore, it is necessary to control this environment in order to maintain the health status of the farms and to preserve the welfare of the animals.
- Des stratégies de traitements antibiotiques sont utilisées depuis très longtemps mais posent deux problèmes majeurs : d’une part, les résistances générées par l’utilisation trop régulière d’antibiotique, et d’autre part, un problème de santé publique par la consommation de chaires animales comprenant de nombreux antibiotiques. Antibiotic treatment strategies have been used for a very long time but pose two major problems: on the one hand, the resistance generated by the too regular use of antibiotics, and on the other hand, a public health problem by the consumption of chairs. including many antibiotics.
- Aussi, est-il nécessaire de trouver des alternatives aux antibiotiques. Also, is it necessary to find alternatives to antibiotics.
- Une approche plus récente a été développée visant à utiliser des flores bactériennes permettant de réguler les flores pathogènes retrouvées dans les élevages. On peut citer par exemple des applications dans l’environnement comme dans la demande EP0852114, où des bactéries non pathogènes sont utilisées pour proposer un procédé de désinfection systématique des bâtiments destinés à l’élevage d’un troupeau.A more recent approach has been developed to use bacterial flora to regulate pathogenic flora found on farms. Examples which may be mentioned are applications in the environment as in application EP0852114, in which non-pathogenic bacteria are used to propose a method of systematic disinfection of buildings intended for rearing a herd.
- D’autres approches sont utilisées et visent à traiter directement les animaux par voie cutanée avec des bactéries non pathogènes. On peut citer notamment la demande CN104307012 qui propose des compositions déodorantes et assainissantes pouvant être pulvérisées sur les volailles, ou encore la demande CN103773721 qui propose des solutions probiotiques limitant les risques de diarrhée chez le nouveau-né, les exemples d’application étant par exemple une pulvérisation au niveau des mamelles de la mère.Other approaches are used and aim to directly treat animals dermally with non-pathogenic bacteria. There may be mentioned in particular the application CN104307012 which proposes deodorant and sanitizing compositions that can be sprayed on poultry, or the application CN103773721 which proposes probiotic solutions limiting the risk of diarrhea in the newborn, the examples of application being for example spraying at the mother's teats.
- Toutefois, malgré ces différentes méthodes, les résultats sanitaires ne semblent pas satisfaisants.However, despite these different methods, the health results do not seem satisfactory.
- Aussi, existe-t-il un besoin de trouver une solution pour maintenir l’environnement d’élevage des animaux terrestre sains, afin de favoriser leur santé et ainsi leur bien-être, ce qui favorisera la productivité des animaux d’élevage.Also, there is a need to find a solution to maintain the environment of healthy terrestrial animals breeding, in order to promote their health and thus their well-being, which will favor the productivity of farm animals.
- L’invention a pour but de palier les inconvénients susmentionnés.The object of the invention is to overcome the aforementioned drawbacks.
- Un autre but de l’invention est de fournir une nouvelle méthode efficace pour maintenir la santé et le bien-être des animaux d’élevage terrestre.Another object of the invention is to provide a new and effective method for maintaining the health and welfare of terrestrial livestock.
- Un autre but de l’invention est de fournir un kit utilisable directement par les éleveurs.Another object of the invention is to provide a kit that can be used directly by breeders.
- Aussi, l’invention concerne une composition bactérienne comprenant au moins une souche de bactérie du genre Bacillus , notamment de Bacillus subtilis et au moins une souche de bactérie lactique, notamment au moins une souche de Lactoco c cus lactis, pour son utilisation dans le cadre de la prévention ou du traitement des infections pathogènes affectant la santé des animaux d’élevage terrestres, Also, the invention relates to a bacterial composition comprising at least one strain of bacteria of the genus Bacillus , in particular Bacillus subtilis and at least one strain of lactic bacteria, in particular at least one strain of Lactoco c cus lactis , for use in the prevention or treatment of pathogenic infections affecting the health of terrestrial livestock,
- ladite composition étant : said composition being:
- a. Ingérée ou inhalée par lesdits animaux d’élevage terrestres, et at. Ingested or inhaled by said terrestrial livestock, and
- b. soit b. is
- b.1. épandue dans le milieu d’élevage desdits animaux d’élevage terrestres, b.1. spread in the breeding environment of said terrestrial livestock,
- b.2. appliquée sur lesdits animaux d’élevage terrestres, b.2. applied to said terrestrial livestock,
- b.3. épandue dans le milieu d’élevage desdits animaux d’élevage terrestres et appliquée sur lesdits animaux d’élevage terrestres.b.3. spread in the breeding environment of said terrestrial livestock and applied to said terrestrial livestock.
- L’invention repose sur la constatation surprenante faite par les inventeurs qu’un mélange d’au moins une souche de bactéries du genre Bacillus subtilis et au moins une souche du genre Lactococcus lactis, bactéries non pathogènes, agissent en synergie sur la santé animale des animaux terrestres lorsque ledit mélange est à la fois ingéré ou inhalé par les animaux et soit épandu dans le milieu d’élevage, soit appliqué directement sur l’animal, soit les deux. Dans l’invention, l’épandage est assimilé à une dispersion ; ces deux termes peuvent être utilisés uniformément dans l’invention.The invention is based on the surprising finding made by the inventors that a mixture of at least one strain of bacteria of the genus Bacillus subtilis and at least one strain of the genus Lactococcus lactis , non-pathogenic bacteria, act synergistically on the animal health of terrestrial animals when said mixture is both ingested or inhaled by the animals and is spread in the culture medium, or applied directly to the animal, or both . In the invention, the spreading is assimilated to a dispersion; both of these terms can be used uniformly in the invention.
- Les inventeurs ont prouvé une synergie de l’effet protecteur contre des pathogènes lorsque les bactéries décrites dans l’invention (Bacillus et Lactoco c cus) sont à la fois présentes dans les voies digestives ou respiratoires et dans le milieu d’élevage et/ou sur l’animal.The inventors have demonstrated a synergistic protective effect against pathogenic when bacteria described herein (Bacillus and Lactoco ECU c) are both present in the gastrointestinal or respiratory tract and in the rearing environment and / or on the animal.
- Dans l’invention on entend par souche bactérienne un ensemble des individus issus de repiquages successifs d'une colonie bactérienne. Une souche bactérienne est une partie d'une espèce bactérienne différente des autres bactéries de la même espèce par une différence mineure mais identifiable.In the invention, bacterial strain is understood to mean a group of individuals from successive subcultures of a bacterial colony. A bacterial strain is a part of a bacterial species different from other bacteria of the same species by a minor but identifiable difference.
- Dans l’invention, on entend par infections pathogènes, les infections par les microorganismes suivants : Salmonelles, Staphylocoques, Streptocoques, Clostridium, Listeria, Arcanobacterium, Mycoplasme, Klebsielle, Pasteurelles, Bordetelles, Campylobacter, Brachyspire, Escherichia coli et/ou responsables des pathologies suivantes : entérites, salmonellose, ulcères, boiteries infectieuses, rhinite, mammite, épidermites, nécroses, pasteurellose, bordetellose, listériose. In the invention, the term "pathogenic infections" means infections by the following microorganisms: Salmonella, Staphylococcus, Streptococci, Clostridium, Listeria, Arcanobacterium, Mycoplasma, Klebsielle, Pasteurella, Bordetella, Campylobacter, Brachyspire, Escherichia coli and / or responsible for pathologies enteritis, salmonellosis, ulcers, infectious lameness, rhinitis, mastitis, epidermitis, necrosis, pasteurellosis, bordetellosis, listeriosis.
- Il est particulièrement avantageux dans l’invention que la composition susmentionnée comprenne au moins deux souches de Bacillus, et notamment au moins deux souches différentes de Bacillus et au moins une souche de bactérie lactique, notamment de L actococcus lactis.It is particularly advantageous in the invention that the aforementioned composition comprises at least two strains of Bacillus , and in particular at least two different strains of Bacillus. and at least one strain of lactic acid bacterium, in particular L actococcus lactis .
- Il est encore plus avantageux dans l’invention que la composition susmentionnée comprenne au moins trois souches de Bacillus, et notamment au moins trois souches différentes de Bacillus, et au moins une souche de bactérie lactique, notamment de L actococcus lactis.It is even more advantageous in the invention that the aforementioned composition comprises at least three strains of Bacillus , and in particular at least three different strains of Bacillus , and at least one strain of lactic acid bacterium, especially L actococcus lactis .
- Il est encore plus avantageux dans l’invention que la composition susmentionnée comprenne au moins quatre souches de Bacillus, et notamment au moins quatre souches différentes de Bacillus et au moins une souche de bactérie lactique, notamment de L actococcus lactis .It is even more advantageous in the invention that the aforementioned composition comprises at least four strains of Bacillus , and in particular at least four different strains of Bacillus. and at least one strain of lactic acid bacterium, in particular L actococcus lactis .
- Dans l’invention on distinguera une application dans l’environnement (E), en référence à l’épandage dans l’environnement, d’une application externe sur l’animal (A) en référence à l’application sur l’animal, et d’une application interne (I), en référence à l’ingestion ou l’inhalation.In the invention there will be distinguished an application in the environment (E), with reference to the spreading in the environment, of an external application on the animal (A) with reference to the application on the animal, and an internal application (I), with reference to ingestion or inhalation.
- L’application E permet de contrôler et/ou d’orienter la flore bactérienne de l’environnement des animaux d’élevage terrestre en diminuant la pression pathogène. Elle peut se faire en nébulisation ou pulvérisation des bâtiments d’élevage, incorporation à la litière… On observe moins de contamination des animaux car il y a moins de foyers pathogènes. Une telle application joue sur les pathogènes vivants ou survivants dans l’environnement. Les modes d’épandage dans l’environnement sont la pulvérisation, la nébulisation, le recouvrement des surfaces par des mousses ou encore le saupoudrage de poudre. The application E makes it possible to control and / or guide the bacterial flora of the environment of terrestrial livestock by reducing the pathogenic pressure. It can be done in nebulization or spraying of livestock buildings, incorporation into litter ... There is less contamination of animals because there are fewer pathogenic foci. Such an application plays on living or surviving pathogens in the environment. The modes of spreading in the environment are the spraying, the nebulization, the covering of surfaces by foams or the dusting of powder.
- L’épandage se fait généralement sur les surfaces de l’élevage, principalement sur les surfaces en contact direct ou potentiel avec les animaux, de préférence sur des surfaces nettoyées et désinfectées, après avoir attendu la fin de la rémanence des produits désinfectants. Spreading is generally carried out on the surfaces of the farm, mainly on surfaces in direct or potential contact with the animals, preferably on cleaned and disinfected surfaces, after waiting for the end of the remanence of the disinfectants.
- L’épandage dans l’environnement est réalisé au moins une fois après un vide sanitaire, après un protocole de nettoyage / désinfection ou avant arrivée des animaux. Il est particulièrement avantageux de pratiquer une application répétée toutes les semaines.Spreading in the environment is carried out at least once after a crawl space, after a protocol of cleaning / disinfection or before arrival of the animals. It is particularly advantageous to practice a repeated application every week.
- L’application A permet de contrôler ou d’orienter le microbisme de l’animal en diminuant la pression pathogène. L’application peut se faire en nébulisation, pulvérisation, trempage de l’animal terrestre. Cela génère moins de contamination des animaux car il y a moins de foyers pathogènes directement sur l’animal.Application A makes it possible to control or orient the microbism of the animal by reducing the pathogenic pressure. The application can be done by nebulization, spraying, soaking of the terrestrial animal. This generates less contamination of the animals because there are fewer pathogenic foci directly on the animal.
- L’application sur l’animal peut se faire par pulvérisation, nébulisation, trempage, pédiluve, mousse, selon la zone de l’animal sur laquelle il est souhaité de déposer la composition selon l’invention.The application to the animal can be done by spraying, nebulization, soaking, footbath, foam, depending on the area of the animal on which it is desired to deposit the composition according to the invention.
- Il est avantageux d’appliquer au moins une fois la composition sur l’animal le plus tôt possible dans la vie de l’animal ou lors de l’arrivée dans un nouvel environnement. De préférence l’application est répétée toutes les semaines.It is advantageous to apply the composition at least once to the animal as early as possible in the life of the animal or upon arrival in a new environment. Preferably the application is repeated every week.
- L’application I permet de contrôler et/ou d’orienter le microbisme digestif en diminuant la pression pathogène et/ou en stimulant les défenses naturelles des animaux. L’application peut se faire via l’eau de boisson, l’alimentation ou tous les moyens permettant à l’animal d’ingérer la composition de l’invention. De cette manière, la composition selon l’invention joue directement sur la santé digestive des animaux d’élevage terrestre, ce qui limite l’entrée d’autres pathogènes.Application I makes it possible to control and / or guide digestive microbism by decreasing the pathogenic pressure and / or by stimulating the animals' natural defenses. The application can be via the drinking water, the diet or any means allowing the animal to ingest the composition of the invention. In this way, the composition according to the invention directly affects the digestive health of terrestrial livestock, which limits the entry of other pathogens.
- L’application I peut également être une application par inhalation, par nébulisation ou par pulvérisation dans les voies respiratoires.Application I may also be an application by inhalation, nebulization or spraying in the respiratory tract.
- L’application I devrait être démarrée le plus tôt possible, avant le début de la phase à risque sanitaire et pendant toute la durée de la période à risque sanitaire. Il est recommandé de renouveler l’application au minimum tous les 10 jours, au mieux tous les jours.Application I should be started as soon as possible, before the start of the health risk phase and for the duration of the health risk period. It is recommended to renew the application at least every 10 days, at best every day.
- Dans l’invention, on entend par animaux d’élevage terrestre, tous les animaux n’étant pas aquatique, et pouvant servir directement ou indirectement à la nutrition humaine ou animale. Cela concerne notamment les bovins, les ovins, les porcins, les caprins, les lapins, les équidés, les insectes mais également les oiseaux d’élevages tels que les poules, les canards, les oies, les dindes, les cailles et autres gallinacés d’élevage.In the invention, the term terrestrial livestock means all the animals are not aquatic, and can be used directly or indirectly for human or animal nutrition. This concerns in particular cattle, sheep, pigs, goats, rabbits, equines, insects but also farmed birds such as chickens, ducks, geese, turkeys, quails and other guinea-pigs. 'breeding.
- Avantageusement, l’invention concerne une composition pour son utilisation susmentionnée, dans laquelle la composition bactérienne comprend :Advantageously, the invention relates to a composition for its aforementioned use, wherein the bacterial composition comprises:
- - au moins l'une des trois souches de Bacillus subtilis suivantes : NOL01, NOL02, NOL03, lesdites souches étant respectivement déposées à la CNCM sous le numéro CNCM I - 4606, CNCM I-5043 et CNCM I - 4607, et at least one of the following three Bacillus subtilis strains: NOL01, NOL02, NOL03, said strains being respectively deposited at the CNCM under the number CNCM I-4606, CNCM I-5043 and CNCM I-4607, and
- - au moins la souche de bactérie lactique: Lactococcus lactis spp lactis 1 souche NOL11, ladite souche étant déposée à la CNCM sous le numéro CNCM I - 4609.at least the lactic acid bacterium strain: Lactococcus lactis spp lactis 1 strain NOL11, said strain being deposited at the CNCM under number CNCM I-4609.
- L’invention concerne l’utilisation d’au moins une souche choisie parmi les souches NOL01, NOL02 et NOL03 et au moins la souche NOL11. Aussi, l’invention couvre les 7 combinaisons suivantes : The invention relates to the use of at least one strain chosen from the NOL01, NOL02 and NOL03 strains and at least the NOL11 strain. Also, the invention covers the following 7 combinations:
- - NOL01 et NOL11,- NOL01 and NOL11,
- - NOL02 et NOL11,- NOL02 and NOL11,
- - NOL03 et NOL11,- NOL03 and NOL11,
- - NOL01, NOL02 et NOL11,- NOL01, NOL02 and NOL11,
- - NOL01, NOL03 et NOL11,- NOL01, NOL03 and NOL11,
- - NOL02, NOL03 et NOL11, et- NOL02, NOL03 and NOL11, and
- - NOL01, NOL02, NOL03 et NOL11.- NOL01, NOL02, NOL03 and NOL11.
- Toutes ces souches ont été déposées à la collection nationale des microorganismes (CNCM) à l’Institut Pasteur à Paris, selon le traité de Budapest.All these strains were deposited in the National Collection of Microorganisms (CNCM) at the Pasteur Institute in Paris, according to the Budapest Treaty.
- De manière plus avantageuse, l’invention concerne une composition pour son utilisation susmentionnée, ladite composition comprenant de 104 à 1011 colonies bactériennes de Bacillus et de 104 à 1011 colonies bactériennes de bactéries lactiques, les colonies bactériennes étant en gramme ou en millilitre de composition.More advantageously, the invention concerns a composition for the aforementioned use, said composition comprising 10 April to 10 November bacterial colonies of Bacillus and April 10 to November 10 bacterial colonies of lactic acid bacteria, bacterial colonies being in grams or milliliter of composition.
- En d’autres termes, dans ce mode de réalisation avantageux, si la composition selon l’invention est sous forme liquide, ladite composition comprendra de 104 à 1011 colonies bactériennes de Bacillus par mL de composition, et cela pour chacune des souches lorsque la composition comprend au moins deux souches et de 104 à 1011 colonies bactériennes de bactérie lactique par mL de composition.In other words, in this advantageous embodiment, if the composition according to the invention is in liquid form, said composition will comprise 10 April to 10 November Bacillus bacterial colonies per ml of composition, and this for each of the strains when the composition comprises at least two strains and April 10 to November 10 bacterial colonies of lactic acid bacteria per ml of composition.
- Si par contre la composition selon l’invention est déshydratée ou non aqueuse, ladite composition comprendra de 104 à 1011 colonies bactériennes de Bacillus par g de composition, et cela pour chacune des souches lorsque la composition comprend au moins deux souches et de 104 à 1011 colonies bactériennes de bactérie lactique par g de composition.If, against the composition of the invention is dried or non-aqueous, said composition will comprise 10 April to 10 November Bacillus bacterial colonies per gram of composition, and this for each of the strains when the composition comprises at least two strains and 10 4 to 10 11 bacterial colonies of lactic acid bacteria per gram of composition.
- Dans l’invention, de 104 à 1011 colonies bactériennes signifie : environ 104, environ 5. 104, environ 105, environ 5.105, environ 106, environ 5.106, environ 107, environ 5.107, environ 108, environ 5.108, environ 109, environ 5.109, environ 1010, environ 5.1010 ou environ 1011 colonies bactériennes. In the invention, from 4 to 10 11 bacterial colonies means: about 10 4 , about 5 × 10 4 , about 10 5 , about 5 × 10 5 , about 10 6 , about 5 × 10 6 , about 10 7 , about 5.10 7 , about 10 8 , about 5 × 10 8 , about 10 9 , about 5 × 10 9 , about 10 10 , about 5 × 10 10 or about 10 11 bacterial colonies.
- Les colonies bactériennes s’entendent par mL (ou gramme) de culture bactérienne. L’homme du métier sait aisément comment déterminer ce nombre de bactéries, notamment par comptage soit manuel (en utilisant une lame de Malassez) ou en utilisant un compteur de cellule automatique, ou par dilution puis ensemencement sur gélose et comptage des colonies.Bacterial colonies are per mL (or gram) of bacterial culture. Those skilled in the art will readily know how to determine this number of bacteria, in particular by counting either manually (using a Malassez slide) or by using an automatic cell counter, or by dilution and then agar seeding and colony counting.
- De manière avantageuse, on utilisera de 105 à 1010 colonies bactériennes par individus lors d’une application chez les volailles, de 105 à 1010 colonies bactériennes par individu pour une application chez le porcelet, et de 105 à 101 1 colonies bactériennes par individu pour une application chez le porc.Advantageously, use May 10 to October 10 bacterial colonies by individuals when applied in poultry, May 10 to October 10 bacterial colonies per individual for application in piglets, and 10 May to 10 January 1 bacterial colonies per individual for application in pigs.
- Concernant la dose avantageuse pour une ingestion, celle-ci est de 105 à 1010 colonies bactériennes par animal par application. Regarding the advantageous dose for ingestion, it is 10 5 to 10 10 bacterial colonies per animal per application.
- Pour l’environnement, il est avantageux d’utiliser de 105 à 1011 colonies bactériennes par m2 de surface ou d’environnement à traiter.For the environment, it is advantageous to use 10 May to 10 November bacterial colonies per m 2 of surface or environment to be treated.
- Encore plus avantageusement, l’invention concerne une composition pour son utilisation telle que définie précédemment, où ladite au moins une souche de Bacillus est sous forme sporulée et/ou sous forme végétative.Even more advantageously, the invention relates to a composition for its use as defined above, wherein said at least one strain of Bacillus is in sporulated form and / or in vegetative form.
- Les bactéries lactiques sont quant à elles toujours sous forme végétative. Aussi la composition selon l’invention comprend au moins une souche de Bacillus sous forme sporulée et au moins une souche de bactérie lactique sous forme végétative, ou comprend au moins une souche de Bacillus sous forme végétative et au moins une souche de bactérie lactique sous forme végétative.Lactic acid bacteria are always in vegetative form. Also the composition according to the invention comprises at least one strain of Bacillus spore form and at least one strain of lactic acid bacterium in vegetative form, or comprises at least one strain of Bacillus in vegetative form and at least one strain of lactic acid bacterium in form vegetative.
- Encore plus avantageusement, l’invention concerne une composition pour son utilisation définie ci-dessus, où ladite composition inhalée ou ingérée et ladite composition épandue dans le milieu d’élevage desdits animaux d’élevage terrestres et/ou appliquée sur lesdits animaux d’élevage terrestres, sont utilisées simultanément, séparément ou étalées dans le temps.Even more advantageously, the invention relates to a composition for its use defined above, wherein said inhaled or ingested composition and said composition spread in the medium for rearing said terrestrial livestock and / or applied to said farm animals terrestrial, are used simultaneously, separately or spread over time.
- Il n’est pas nécessaire que les traitements ingérés ou inhalés et les traitements épandus dans l’environnement ou appliqués sur l’animal soient simultanés, c’est-à-dire appliqués en même temps. Il est possible de séparer les applications dans le temps, par exemple commencer à traiter l’environnement de l’animal, puis une fois l’animal introduit dans l’environnement, lui faire ingérer la composition selon l’invention.It is not necessary that ingested or inhaled treatments and treatments applied in the environment or applied to the animal be simultaneous, that is, applied at the same time. It is possible to separate the applications in time, for example start treating the environment of the animal, then once the animal is introduced into the environment, to ingest the composition according to the invention.
- De manière plus avantageuse, l’invention concerne une composition pour son utilisation susmentionnée, où ladite composition est épandue et/ou appliquée préalablement à l’ingestion ou l’inhalation par lesdits animaux d’élevage terrestres.More advantageously, the invention relates to a composition for its aforementioned use, wherein said composition is spread and / or applied prior to ingestion or inhalation by said terrestrial livestock.
- Dans le cas d’un traitement préalable à l’ingestion ou l’inhalation de la composition selon l’invention, l’épandage dans l’environnement se fait selon les doses telles que définies ci-dessus.In the case of a treatment prior to ingestion or inhalation of the composition according to the invention, the spreading in the environment is done according to the doses as defined above.
- Avantageusement, l’invention concerne une composition pour son utilisation susmentionnée, dans laquelle ladite composition consiste essentiellement en les quatre souches bactériennes du groupe constitué par les trois souches NOL01, NOL02, NOL03, lesdites souches étant respectivement déposées à la CNCM sous le numéro CNCM I - 4606, CNCM I-5043 et CNCM I – 4607 et la souche Lactococcus lactis spp lactis 1 souche NOL11, ladite souche étant déposée à la CNCM sous le numéro CNCM I – 4609.Advantageously, the invention relates to a composition for its aforementioned use, wherein said composition consists essentially of the four bacterial strains of the group consisting of the three strains NOL01, NOL02, NOL03, said strains being respectively deposited at the CNCM under number CNCM I - 4606, CNCM I-5043 and CNCM I - 4607 and the Lactococcus strain lactis spp lactis 1 strain NOL11, said strain being deposited at the CNCM under number CNCM I - 4609.
- Cette combinaison est particulièrement avantageuse et décrite dans les exemples.This combination is particularly advantageous and described in the examples.
- Il est particulièrement avantageux que la composition comprenne les trois souches de bactéries Bacillus subtilis susmentionnées, sous forme végétative ou sporulée, et la souche de bactérie lactique susmentionnée, sous forme végétativeIt is particularly advantageous that the composition comprises the above-mentioned three strains of Bacillus subtilis bacteria, in vegetative or sporulated form, and the above-mentioned lactic acid bacteria strain, in vegetative form.
- De manière encore plus avantageuse, l’invention concerne une composition pour son utilisation telle que définie précédemment, où ladite composition est épandue par m2 à raison de 105 à 1011 colonies bactériennes. Even more advantageously, the invention relates to a composition for its use as defined above, wherein said composition is spread by m 2 in the proportion of 10 5 to 11 bacterial colonies.
- Dans le cadre de l’application dans l’environnement des animaux d’élevage, il est avantageux que la composition soit dispersée ou épandue à raison de 105 à 1011 colonies bactériennes pour une composition sèche ou déshydratée, ou 105 à 1011 colonies bactériennes pour une composition liquide par m2 de surface ou de milieu à traiter.In the context of the application in the environment of farm animals, it is advantageous for the composition to be dispersed or spread at a rate of 10 5 to 10 11 bacterial colonies for a dry or dehydrated composition, or 10 5 to 10 11 bacterial colonies for a liquid composition per m 2 of surface or medium to be treated.
- Toujours de manière avantageuse, l’invention concerne une composition pour son utilisation susmentionnée, où l’environnement est nettoyé et désinfecté et les animaux terrestres sont lavés préalablement à l’épandage dans le milieu d’élevage desdits animaux d’élevage terrestres et/ou l’application sur lesdits animaux d’élevage terrestres.Still advantageously, the invention relates to a composition for its use mentioned above, where the environment is cleaned and disinfected and the terrestrial animals are washed prior to the application in the breeding environment of said terrestrial livestock and / or the application on said terrestrial livestock.
- Il peut être avantageux de nettoyer l’environnement et/ou les animaux avant d’utiliser la composition selon l’invention. Ce nettoyage peut être assimilé à une désinfection. Avant implantation des flores de la composition selon l’invention, si un nettoyage ou une désinfection est opéré, meilleure sera l’efficacité des bactéries, et meilleur sera le bénéfice pour les animaux. Les surfaces peuvent être traitées par tout moyen de nettoyage connu de l’homme du métier. En ce qui concerne le nettoyage des animaux, il est possible de les laver, les baigner, voire de les shampooiner.It may be advantageous to clean the environment and / or the animals before using the composition according to the invention. This cleaning can be assimilated to a disinfection. Before implantation of the flora of the composition according to the invention, if a cleaning or disinfection is operated, the better the effectiveness of the bacteria, and the better the benefit for the animals. Surfaces may be treated by any cleaning means known to those skilled in the art. Regarding the cleaning of animals, it is possible to wash, bathe or even shampoo.
- L’invention concerne en outre une trousse à destination des animaux d’élevage terrestres contre les infections pathogènes affectant la santé et le bien–être des animaux d’élevage comprenantThe invention further provides a kit for terrestrial livestock against pathogenic infections affecting the health and welfare of farm animals including
- - une composition telle que définie dans l’une quelconque des revendications précédentes, et a composition as defined in any one of the preceding claims, and
- - au moins un agent de biosécurité et/ou au moins un agent de biocontrôle- at least one biosecurity agent and / or at least one biocontrol agent
- Dans l’invention on entend par agent de biosécurité un agent chimique ou physique capable de réduire le nombre ou d’inactiver des microorganismes et leurs biofilms. Il s’agit par exemple de désinfectants, de détergents, de rayonnements UV, ou encore de la vapeur d’eau ou tout autre agent biocide. Cette liste n’est pas limitative, et l’homme du métier connait les agents de biosécurité. In the invention, the term "biosafety agent" means a chemical or physical agent capable of reducing the number or inactivating microorganisms and their biofilms. These include, for example, disinfectants, detergents, UV radiation, or steam or any other biocidal agent. This list is not exhaustive, and the person skilled in the art knows the biosecurity agents.
- Dans l’invention on entend par agent de biocontrôle toute composition d’organismes vivants ou issue d’organismes vivants, ou composition de substances naturelles ayant pour but de protéger les animaux d’un point de vue sanitaire (des macro-organismes (insectes, nématodes, etc.), des micro-organismes (virus, bactéries, phages ou champignons), des médiateurs chimiques (phéromones), ou des substances naturelles d’origine minérale, végétale ou animale telles que par exemple des huiles essentielles).In the invention, the term "biocontrol agent" is intended to mean any composition of living organisms or organisms derived from living organisms, or a composition of natural substances intended to protect animals from a health point of view (macro-organisms (insects, nematodes, etc.), micro-organisms (viruses, bacteria, phages or fungi), chemical mediators (pheromones), or natural substances of mineral, vegetable or animal origin, such as, for example, essential oils).
- Dans l’invention, il est proposé de fournir un kit qui comprend la composition telle que définie ci-dessus, et notamment une composition comprenant les bactéries NOL01, NOL02, et NOL03 avec la bactérie NOL11, et en parallèle au moins un agent de biocontrôle et/ou de biosécurité.In the invention, it is proposed to provide a kit which comprises the composition as defined above, and in particular a composition comprising the bacteria NOL01, NOL02, and NOL03 with the bacterium NOL11, and in parallel at least one biocontrol agent. and / or biosecurity.
- Ce kit permet à l’utilisateur de disposer des moyens d’appliquer la composition selon l’invention, et le cas échéant, au préalable, ou après le traitement avec la composition selon l’invention, d’utiliser un ou plusieurs autres agents tels que définis ci-dessus.This kit allows the user to have the means to apply the composition according to the invention, and if necessary, beforehand, or after the treatment with the composition according to the invention, to use one or more other agents such as as defined above.
- Il est notable que pour conserver l’effet de la composition selon l’invention, l’agent susmentionné ne doit pas être utilisé simultanément avec la composition selon l’invention s’il a un effet antibactérien.It is notable that to maintain the effect of the composition according to the invention, the aforementioned agent should not be used simultaneously with the composition according to the invention if it has an antibacterial effect.
- L’invention sera mieux comprise à la lumière des trois exemples et des 13 figures suivantes :The invention will be better understood in the light of the three examples and the following 13 figures:
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- EXEMPLE EXAMPLE 11 – application - application dans l’environnementin the environment : litière des veaux d’élevage: litter of farmed calves
- Dans ce premier exemple, les inventeurs cherchent à montrer si une application de la composition selon l’invention dans l’environnement ou sur la litière, seule, à un intérêt pour la prévention des infections pathogènes.In this first example, the inventors seek to show whether an application of the composition according to the invention in the environment or on the litter, alone, to an interest for the prevention of pathogenic infections.
- Cet essai est réalisé sur la partie veaux d’un élevage de vaches laitières Holstein en France dans le département d’Ille-et-Vilaine.This test is performed on the calves part of a Holstein dairy farm in France in the department of Ille-et-Vilaine.
- Le produit testé est une association de bactéries vivantes (souches de Bacillus subtilis et de Lactococcus lactis) dans leur milieu de culture, apporté congelé à l’élevage, devant être dilué dans de l’eau avant pulvérisation. The tested product is an association of living bacteria (strains of Bacillus subtilis and Lactococcus lactis ) in their culture medium, brought frozen at the farm, to be diluted in water before spraying.
- L’essai est réalisé dans le bâtiment des veaux avec un lot témoin et un lot où le produit sera pulvérisé dans l’environnement et sur la litière. L’impact du produit sera étudié au cours du temps. The test is performed in the calf building with a control lot and a lot where the product will be sprayed in the environment and on the litter. The impact of the product will be studied over time.
- L’influence du produit sera mesurée sur les qualités chimiques de la litière, la propreté des animaux et le bilan sanitaire du lot (fréquence et nature des pathologies, frais vétérinaires) au cours de temps. The influence of the product will be measured on the chemical qualities of the litter, the cleanliness of the animals and the sanitary balance of the batch (frequency and nature of pathologies, veterinary costs) over time.
- MATERIEL ET METHODESMATERIAL AND METHODS
- a - Animauxa - Animals
- 16 veaux d’élevage Holstein de 6 semaines à 5 mois et demi répartis par 3, 4 ou 5 selon leur âge dans 4 cases de 15m² en aire paillée, es deux premières cases étant séparées des deux autres par un mur.16 Holstein breeding calves from 6 weeks to 5 ½ months divided by 3, 4 or 5 according to their age in 4 squares of 15m² in straw area, the first two boxes being separated from the other two by a wall.
- b -Produit utilisé et matériel b -Product used and material
- Une dose de composition est conditionnée dans deux flacons distincts de 20 ml : A dose of composition is packaged in two separate bottles of 20 ml:
- • Le flacon B contenant des bacillus (NOL01, NOL02, NOL03)• Bottle B containing bacillus (NOL01, NOL02, NOL03)
- • Le flacon L contenant les Lactococcus (NOL11)• Bottle L containing Lactococcus (NOL11)
- Les flacons sont apportés congelés en élevage.The flasks are brought frozen in breeding.
- L’eau utilisée lors de la pulvérisation ne doit pas contenir de chlore. Un traitement préalable de l’eau au thiosulfate de sodium peut donc être nécessaire. Water used during spraying must not contain chlorine. Pre-treatment of water with sodium thiosulfate may therefore be necessary.
- Le matériel nécessaire pour le traitement et les mesures est le suivant :The material needed for processing and measurements is as follows:
- - 8 boites de 2 flacons de composition- 8 boxes of 2 bottles of composition
- - Du thiosulfate de sodium - Sodium thiosulfate
- - 1 pulvérisateur- 1 sprayer
- - 1 ruban bovimétrique- 1 bovimetric tape
- c -Dispositif expérimentalc -Experimental device
- AVANT LE LANCEMENT DES ESSAIS : BEFORE LAUNCHING TESTS:
- - On prendra garde à vérifier le bon fonctionnement du pulvérisateur.- Be careful to check the correct operation of the sprayer.
- - Surface totale de l’aire paillée (témoin + essai) : 60 m2, soit environ 4 m2 par animal - Total area of the straw area (control + test): 60 m 2 , approximately 4 m 2 per animal
- - Surface traité avec la composition selon l’invention : 2 cases de 15 m² Surface treated with the composition according to the invention: 2 boxes of 15 m²
- - Surface témoin : 2 cases de 15 m²- Sample surface: 2 boxes of 15 m²
- Séparation des cases témoins et essais par un mur.Separation of control boxes and tests by a wall.
- Le poids des veaux est estimé au début de l’essai grâce à un ruban bovimétrique. La répartition des veaux dans chaque case a été réalisé pour obtenir un poids vif total comparable entre les cases témoins et essais.The weight of the calves is estimated at the beginning of the test using a bovimetric tape. The distribution of calves in each box was carried out to obtain a comparable total live weight between the control and test boxes.
- d - Déroulement de l’essai et mesuresd - Conduct of the test and measures
- L’essai a duré 2 mois, période entre deux curages. The test lasted 2 months, period between two cleaning sessions.
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SemSem 1616 SemSem 1717 SemSem 1818 SemSem 1919 SemSem 2020 SemSem 2121 SemSem 2222 SemSem 2323 SemSem 2424 Curageflushing xx xx PulvérisationSpray des cases 3 et 4 boxes 3 and 4 XX XX XX XX XX XX XX XX - La première application du produit est réalisée après le curage en semaine 16. Les premières mesures sont réalisées juste avant la première application des flores. Ensuite les mesures sont réalisées chaque semaine avant application du produit. Fin des applications du produit en semaine 23 et dernière mesure juste avant le deuxième curage en semaine 24.The first application of the product is carried out after the cleaning in week 16. The first measurements are made just before the first application of flora. Then the measurements are made every week before application of the product. End of the applications of the product in week 23 and last measurement just before the second cleaning in week 24.
- e - Applicatione - Application
- Mode d’emploi : à appliquer sous forme liquide. Une dose de produit (constituée des flacons B et L) est nécessaire pour chaque pulvérisation hebdomadaire.Instructions for use: to be applied in liquid form. A dose of product (consisting of bottles B and L) is required for each weekly spray.
- 1 L de composition diluée est utilisé pour traiter les 30 m² de l’essai veaux.1 L of diluted composition is used to treat the 30 m² calf test.
- Procédure de préparation de la solution :Procedure for preparing the solution:
- • Laisser décongeler le produit à température ambiante entre 15 et 25 °C jusqu’à liquéfaction complète (env. 1h ± 15 minutes) • Let the product defrost at room temperature between 15 and 25 ° C until complete liquefaction (about 1h ± 15 minutes)
- • Préparer 10L d’eau et ajouter 160mg de thiosulfate de sodium (si l’eau est traitée au chlore, ajouter du thiosulfate de sodium à hauteur de 16 mg/L d’eau. Si l’eau est traitée au peroxyde d’hydrogène, utiliser impérativement de l’eau minérale ou de l’eau non traitée).• Prepare 10L of water and add 160mg of sodium thiosulphate (if the water is treated with chlorine, add sodium thiosulphate at 16mg / L of water If the water is treated with hydrogen peroxide , use imperatively mineral water or untreated water).
- • Enlever la capsule et retirer le bouchon des flacons• Remove the cap and remove the cap from the vials
- • Verser les deux flacons du produit dans l’eau traitée• Pour the two bottles of the product into the treated water
- • Mélanger pour homogénéiser le produit puis laisser reposer pendant 15 minutes• Mix to homogenize the product and let stand for 15 minutes
- • Dans un pulvérisateur n’ayant pas contenu de désinfectant, verser 1L de la composition selon l’invention, ladite composition étant diluée, et pulvériser sur la litière et l’ensemble des surfaces des cases 3 et 4. • In a sprayer that has not contained disinfectant, pour 1L of the composition according to the invention, said composition being diluted, and spray on the litter and all the surfaces of the cells 3 and 4.
- • Cette application sera à renouveler toutes les semaines de l’essai (8 au total).• This application will be renewed every week of the trial (8 in total).
- Pendant l’essai, aucune désinfection ne sera réalisée dans le bâtiment.During the test, no disinfection will be carried out in the building.
- f - Pratiques de paillage f - Mulching practices
- L’éleveur garde ses pratiques de paillage habituelles pendant toute la durée de l’essai.The breeder keeps his usual mulching practice for the duration of the test.
- • Apporter 1 round de paille pour 30 m2 après curage • Bring 1 round of straw for 30 m 2 after cleaning
- • Paillage 2 à 3 fois par semaine• Mulching 2 to 3 times a week
- • Répartir les quantités de paille de manière identique dans chacune des cases. • Distribute the quantities of straw in the same way in each box.
- • Lors des journées de mesures/traitement, le paillage est réalisé après ces pratiques. • During the measurement / treatment days, mulching is carried out after these practices.
- g - Mesures et Analysesg - Measurements and Analyzes
- Date des prélèvements et mesures : Date of sampling and measurements:
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Sem16SEM16 Sem17Sem17 Sem18Sem18 Sem19Sem19 Sem20Sem20 Sem21Sem21 Sem22Sem22 Sem23Sem23 Sem24Sem24 Curageflushing xx xx MS de la litièreMS of the litter xx xx xx xx xx T° de la litièreT ° of litter xx xx xx xx xx xx xx xx xx Hauteur de litièreLitter height xx xx xx xx xx xx xx xx xx - • Mesures de température • Temperature measurements
- Réaliser une mesure de T° hebdomadaire sur l’horizon 0 – 20 cm dès le premier curage à la surface de la paille et en profondeur (indiquer la profondeur de la première mesure pour réaliser la même tout au long de l’essai, si l’épaisseur augmente, une troisième mesure plus profonde pourra être prise (longueur totale de la sonde). Make a measurement of weekly T ° on the horizon 0 - 20 cm from the first cleaning on the surface of the straw and in depth (indicate the depth of the first measurement to achieve the same throughout the test, if As the thickness increases, a third, deeper measurement may be taken (total length of the probe).
- • Mesures de MS• MS measurements
- A partir du premier curage, une fois toutes les deux semaines, réaliser des prélèvements de litière (3 – 4 poignées pour un total de 200g minimum) sur l’horizon 0 – 20 cm, et mesurer la MS (envoi des échantillons au laboratoire). Les semaines avec curage, réaliser la mesure au jour du curage, juste avant le début des opérations.From the first cleaning, once every two weeks, take samples of litter (3 - 4 handles for a total of 200g minimum) on the horizon 0 - 20 cm, and measure the MS (sending samples to the laboratory) . The weeks with cleaning, make the measurement in the day of cleaning, just before the start of operations.
- Pour chaque prélèvement, effectuer un prélèvement à tous les points de prélèvement, et rassembler les échantillons par case. On obtient donc 4 échantillons de 1kg minimum. Homogénéiser avant envoi à l’analyse et identifier de la manière suivante : numéro de case (1, 2, 3 et 4) / date de prélèvement / analyse (MS)For each sample, take a sample at all sampling points, and collect the samples per cell. We thus obtain 4 samples of 1kg minimum. Homogenize before sending to the analysis and identify as follows: case number (1, 2, 3 and 4) / sampling date / analysis (MS)
- • Mesures sur les animaux • Measures on animals
- Sanitaire : demander à l’éleveur de noter les problèmes sanitaires apparus en cours d’essai + les frais vétérinaires pour chaque période (diarrhée, problème respiratoire ou autre).Sanitary: ask the farmer to note the health problems that have appeared during the trial + the veterinary fees for each period (diarrhea, respiratory problem or other).
- Résultats et conclusion :Results and conclusion:
- Les résultats de températures de la litière ainsi que la matière sèche sont équivalents entre les deux lots. The litter temperature results as well as the dry matter are equivalent between the two batches.
- Quelques diarrhées observées, mais aucune différence significative entre le lot témoin et le lot essai (traité avec la composition selon l’invention), notamment en qualité de fèces et en termes de propreté des cases où les résultats sont équivalents entres les lots. Some diarrhea observed, but no significant difference between the control group and the test batch (treated with the composition according to the invention), especially as faeces and in terms of cleanliness of the boxes where the results are equivalent between batches.
- Aussi, l’application dans l’environnement seule n’est pas suffisante pour améliorer la condition de vie des animaux.Also, the application in the environment alone is not sufficient to improve the living condition of animals.
- CONTEXTE ET OBJECTIFSCONTEXT AND OBJECTIVES
- Dans ce second exemple l’objectif est de déterminer si l’application sur l’animal peut avoir un meilleur effet. L’objectif de cet essai est de tester l’application de la flore de barrière (combinaison congelée de Bacillus et Lactococcus) dès le couvoir, sur les poussins à J0, afin d’étudier son impact sur la qualité sanitaire des lots. L’essai sera réalisé sur des poussins à destination de 20 élevages, qui seront comparés à des lots témoins contemporains. Dans chaque élevage en essai, seuls les poussins d’un bâtiment seront traités en couvoir, les poussins du second bâtiment n’auront subi aucun traitement (témoin).In this second example the objective is to determine if the application on the animal can have a better effect. The objective of this trial is to test the application of the barrier flora (frozen combination of Bacillus and Lactococcus ) from the hatchery, on chicks at D0, in order to study its impact on the sanitary quality of the batches. The test will be conducted on chicks for 20 farms, which will be compared to contemporary control batches. In each farm under test, only the chicks of a building will be treated in hatchery, the chicks of the second building will have undergone no treatment (witness).
- Le but est de vérifier un effet sur la baisse de mortalité, l’amélioration sanitaire, l’amélioration des indices zootechniques. The aim is to verify an effect on the decrease of mortality, health improvement, improvement of zootechnical indices.
- On applique la composition selon l’invention (souches de Bacillus subtilis NOL01, NOL02, NOL03 et de Lactococcus lactis NOL03) directement sur les poussins après sexage. Les doses seront décongelées dans un bain-marie de 27°C pendant 9 minutes 30.The composition according to the invention is applied (strains of Bacillus subtilis NOL01, NOL02, NOL03 and Lactococcus lactis NOL03) directly on the chicks after sexing. The doses will be thawed in a 27 ° C water bath for 9 minutes.
- Elles seront ensuite diluées dans 3L d’eau minérale pour 30 000 poussins, soit 0,1mL par poussin. Cette préconisation tient compte du volume d’eau utilisé pour une vaccination par pulvérisation soit 0,2 à 0,3mL.They will then be diluted in 3L of mineral water for 30,000 chicks, or 0.1mL per chick. This recommendation takes into account the volume of water used for a spray vaccination, ie 0.2 to 0.3 mL.
- 100 mâles et 100 femelles sont pesés à J7 dans chaque bâtiment.100 males and 100 females are weighed on day 7 in each building.
- Les paramètres étudiés sont : les consommations d’aliment et d’eau, la mortalité et élimination ainsi que les données de l’abattoir (résultats technico économique, saisies, dénombrement des pododermatites, tarses, lésions cutanées infectées ou LCI et bréchets).The studied parameters are: food and water consumption, mortality and elimination as well as slaughterhouse data (technical and economic results, seizures, enumeration of pododermatitis, tarsi, infected skin lesions or LCI and keel bone).
- Résultats :Results:
- Les résultats observés pour l’indice de consommation corrigé (ICC) sont mentionnés aux figures 1 et 2.The observed results for the corrected consumption index (ICC) are shown in Figures 1 and 2 .
- Avec un test Student : p-value = 0,882, la différence entre les animaux traités et non traités n’est pas significative.With a Student test: p-value = 0.882, the difference between treated and untreated animals is not significant.
- Il en est de même pour la marge poussin aliment (MPA), les tarses brûlés, la mortalité, les traitements antibiotiques, les poids des animaux mentionnés à la figure 3, les pododermatites qui ne sont pas significativement différents entre les témoins et les animaux ayant reçu la composition selon l’invention.It is the same for the chick feed margin (MPA), the burned tarsi, the mortality, the antibiotic treatments, the weights of the animals mentioned in Figure 3, the pododermatitis which are not significantly different between the controls and the animals having received the composition according to the invention.
- Aussi, on constate que l’application sur l’animal seule n’apporte pas de bénéfice à l’animal.Also, it is found that the application on the animal alone does not bring benefit to the animal.
- EXEMPLE 3 – Application dans l’environnement, sur l’animalEXAMPLE 3 - Application in the environment, on the animal : Volailles: Poultry
- OBJECTIFGOAL
- L’objectif de cet essai est de tester l’efficacité de la composition selon l’invention (NOL01, NIL02, NOL03, NOL11) à la fois sur l’animal et dans l’environnement. Le but est d’identifier une utilisation optimale pour la prévention de pathologies (boiteries, colibacilloses, etc.) et/ou la santé intestinale et l’amélioration de la qualité des litières et donc du bien-être animal.The objective of this test is to test the effectiveness of the composition according to the invention (NOL01, NIL02, NOL03, NOL11) both on the animal and in the environment. The aim is to identify an optimal use for the prevention of pathologies (lameness, colibacillosis, etc.) and / or intestinal health and the improvement of litter quality and therefore of animal welfare.
- DISPOSITIF EXPERIMENTALEXPERIMENTAL APPARATUS
- 2 élevages testés. 2 farms tested.
- Le premier élevage testé dispose de 2 bâtiments comparables, avec litière cosses de sarrasin sur sol béton. Il est équipé de pesons. Son eau de boisson est traitée avec 3% d’acide hypochloreux pur à 80% (système de traitement par électrolyse – société DCW).The first breeding tested has 2 comparable buildings, with litter buckwheat husks on concrete floor. It is equipped with load cells. Its drinking water is treated with 3% 80% pure hypochlorous acid (electrolytic treatment system - DCW company).
- Le second élevage dispose de 2 bâtiments comparables, avec litière cosses de sarrasin et copeaux de bois sur sol non bétonné. Il est équipé de pesons. Son eau de boisson est traitée avec du peroxyde d’hydrogène, arrêt du traitement lors de la pulvérisation de la flore.The second breeding has 2 comparable buildings, with litter buckwheat husks and wood chips on unpaved soil. It is equipped with load cells. Its drinking water is treated with hydrogen peroxide, stop treatment when spraying the flora.
- L’influence du produit est mesurée sur les performances de croissance, le bilan sanitaire du lot (fréquence et nature des pathologies, frais vétérinaires), les comptages bactériologiques (litière), les émissions de gaz, la qualité des litières et les critères de bien-être. The influence of the product is measured on the growth performance, the sanitary balance of the batch (frequency and nature of the pathologies, veterinary costs), the bacteriological counts (litter), the gas emissions, the quality of the litters and the criteria of good -be.
- APPLICATIONAPPLICATION
- Application de 5 doses en cours d’élevage.Application of 5 doses during breeding.
- 5 doses réparties de la façon suivante : J0 -J3-J7-J9-J14-J21.5 doses distributed as follows: J0-J3-J7-J9-J14-J21.
- L’ensemencement à J0 est réalisé après la mise en place.Seeding at D0 is performed after placement.
- Pulvériser l’environnement et les animaux.Spray the environment and animals.
- Les bâtiments essais et témoin se distinguent uniquement par l’application ou non de la flore de barrière. Le produit est pulvérisé par brumisation (environ 15-20L d’eau contenant la flore à chaque application, pour 1200 m²). L’eau utilisée est de l’eau de puit artésien non traité.The test and control buildings are distinguished only by the application or not of barrier flora. The product is sprayed by misting (approximately 15-20L of water containing the flora at each application, for 1200 m²). The water used is untreated artesian well water.
- Résultats : Results:
- Le tableau suivant présente une comparaison des résultats entre les bâtiments témoin et essai pour les deux élevages.The following table presents a comparison of results between control and test buildings for both farms.
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Elevage 1 Breeding 1 Elevage 2 Breeding 2 Performances zootechniquesZootechnical performances ICCICC +4 pts+4 pts +2,5 pts+2.5 pts Bien-êtreWelfare Eau /AlimentWater / Food + avant 28j
- après 28j+ before 28 days
- after 28 days-- LitièreLitter - avant 28j- before 28 days - en fin de bande- at the end of the band Matière SècheDry matter - sauf en fin de bande- except at the end of the band - - Pododermatiteslaminitis -- ++ Etat sanitaireSanitary state Traitementstreatments plus de traitements dans le lot témoinmore treatments in the control group plusieurs traitements dans les deux lotsseveral treatments in both lots MortalitéMortality + pour les mâles+ for males + pour les femelles avant 27 j puis -- pour les mâles après 27j + for females before 27 days then - for males after 27 days TriSorting + pour les mâles+ for males -- Tenue sur pattesStand on legs ++ -- Saisiesseizures + pour les femelles+ for females -- BactériologieBacteriology LitièreLitter Pas de différence significativeNo significant difference Pas de différence significativeNo significant difference - Conclusions :conclusions:
- Les applications animal et environnement seules ne suffisent pas à améliorer le bien-être des animaux. En effet, les résultats sur les 2 élevages montrent des effets contradictoires sur les boiteries, la mortalité et les pododermatites, pas de différence significative sur la bactériologie des litières et un indice de consommation dégradé.Animal and environment applications alone are not enough to improve animal welfare. Indeed, the results on the 2 farms show contradictory effects on lameness, mortality and pododermatitis, no significant difference on litter bacteriology and a deteriorated consumption index.
- EXEMPLE 4 – ApplicationEXAMPLE 4 - Application ss dans l’environnement, sur l’animal et in the environment, on the animal and en in ingéréingested : Volailles: Poultry
- Cet essai a pour but de déterminer si une combinaison des applications de la composition décrite à l’exemple 1 dans l’environnement, sur l’animal et d’une ingestion a une efficacité sur la prévention des infections pathogènes.The purpose of this test is to determine whether a combination of the applications of the composition described in Example 1 in the environment, on the animal and ingestion has an efficacy on the prevention of pathogenic infections.
-
- Dispositif expérimentalExperimental apparatus
- Une étude statistique préliminaire a permis de classifier les élevages de volaille chair selon la proximité des résultats zootechniques de deux bâtiments de l’élevage. Sont rentrés dans cette classification, tous les élevages comportant au moins 5 bandes depuis début 2014 pour lesquelles les valeurs de poids, mortalité, ICC et pododermatite ont été renseignées. Cette étude a permis d’établir une liste des 60 élevages comportant des résultats les plus proches entre deux bâtiments. A preliminary statistical study made it possible to classify poultry flocks according to the proximity of the zootechnical results of two livestock buildings. This classification includes all farms with at least 5 bands since the beginning of 2014 for which the weight, mortality, ICC and pododermatite values have been completed. This study established a list of 60 farms with the closest results between two buildings.
- L’essai aura donc lieu dans une vingtaine d’élevages présents sur cette liste avec dans chaque élevage en essai, un bâtiment témoin et un bâtiment essai recevant une application de flores de barrière. Avant l’intégration de chaque élevage dans l’essai, on s’assurera que les deux bâtiments en essai recevront les mêmes lignées d’un même couvoir pendant 2 bandes.The test will therefore take place in about twenty farms on this list with each test farm, a control building and a test building receiving an application of barrier flora. Before the integration of each farm in the test, it will be ensured that the two buildings under test will receive the same lines of the same hatchery during 2 bands.
-
- Produit utilisé et matérielUsed product and material
- L’application du produit se fera avec des pulvérisateurs à accus ou avec les pulvérisateurs des éleveurs utilisés pour la vaccination (après validation du matériel : contenance suffisante, praticité, pas d’utilisation pour des traitements de désinfection ou tout autre produit incompatible). The application of the product will be done with battery sprayers or with the sprayers of the breeders used for the vaccination (after validation of the material: sufficient capacity, practicality, not of use for disinfection treatments or any other incompatible product).
- Le matériel nécessaire pour le traitement et les mesures est le suivant :The material needed for processing and measurements is as follows:
- Boites de 2 flacons de flores Boxes of 2 bottles of flora
- De l’eau minéraleMineral water
- Nébulisateur à accusBattery fogger
- Gants Gloves
- Sur-bottes On Boot
- Cottes Cottes
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- Applicationsapplications
- Deux types d’application sont réalisés : dans le bâtiment + animaux et dans le circuit d’eau.Two types of application are made: in the building + animals and in the water circuit.
- Application environnement et animaux :Environment and animals application:
- Pulvérisation sur : la litière, les mangeoires, les abreuvoirs et sur les animaux. Spray on: bedding, feeders, troughs and on animals.
- 1 dose pour 1000 à 1200 m² ou 1 dose pour 20 à 24 000 poussins. 1 dose for 1000 to 1200 m² or 1 dose for 20 to 24 000 chicks.
- Application : J0 (juste après la mise en place), J10 et J20.Application: J0 (just after setting up), J10 and J20.
- Procédure de préparation de la solution :Procedure for preparing the solution:
- Laisser décongeler le produit à température ambiante entre 15 et 25 °C jusqu’à liquéfaction complète (env. 1h ± 15 minutes) ou 5 minutes dans un bain-marie de 30°C.Thaw the product at room temperature between 15 and 25 ° C until complete liquefaction (about 1h ± 15 minutes) or 5 minutes in a 30 ° C water bath.
- Verser 10L d’eau dans le réservoir du pulvérisateur (si l’eau est traitée au chlore, ajouter du thiosulfate de sodium à hauteur de 16 mg/L d’eau. Si l’eau est traitée au peroxyde d’hydrogène, utiliser impérativement de l’eau minérale ou de l’eau non traitée).Pour 10L of water into the spray tank (if the water is treated with chlorine, add sodium thiosulphate at a rate of 16 mg / L of water) If the water is treated with hydrogen peroxide, it is essential to use mineral water or untreated water).
- Enlever la capsule et retirer le bouchon des flaconsRemove the cap and remove the cap from the vials
- Verser les deux flacons du produit dans l’eau (traitée ou minérale)Pour the two bottles of the product into the water (treated or mineral)
- Mélanger pour homogénéiser le produit puis laisser reposer pendant 15 minutes avant de commencer la nébulisation. Pulvériser la solution dans l’ensemble du bâtiment et sur les animaux.Mix to homogenize the product and let stand for 15 minutes before starting nebulization. Spray the solution throughout the building and on the animals.
- Application dans le circuit d’eau :Application in the water circuit:
- Arrêt du traitement de l’eau (ou neutralisation par du thiosulfate de sodium si l’eau est chlorée). Stop the treatment of water (or neutralization with sodium thiosulphate if the water is chlorinated).
- 1 dose pour 20 à 24 000 poussins. 1 dose for 20 to 24 000 chicks.
- Application à : J0 - J1 - J2 - J10 - J20 - J30 - J40Application to: J0 - J1 - J2 - J10 - J20 - J30 - J40
- Procédure de préparation de la solution :Procedure for preparing the solution:
- Laisser décongeler le produit à température ambiante entre 15 et 25 °C jusqu’à liquéfaction complète (env. 1h ± 15 minutes) ou 5 minutes dans un bain-marie de 30°C.Thaw the product at room temperature between 15 and 25 ° C until complete liquefaction (about 1h ± 15 minutes) or 5 minutes in a 30 ° C water bath.
- Remplir le réservoir d’environ 100L d’eau pour les applications jusqu’à J10 et 300L pour les autres (si l’eau est traitée au chlore, ajouter du thiosulfate de sodium à hauteur de 16 mg/L d’eau. Si un autre traitement de l’eau est réalisé, l’arrêter les jours de traitement).Fill the tank with approximately 100L of water for applications up to J10 and 300L for others (if the water is treated with chlorine, add sodium thiosulphate at a rate of 16 mg / L of water. other water treatment is performed, stop it on treatment days).
- Enlever la capsule et retirer le bouchon des flaconsRemove the cap and remove the cap from the vials
- Verser les deux flacons du produit dans l’eau Pour the two bottles of the product into the water
- Mélanger pour homogénéiser le produit Mix to homogenize the product
- Si un traitement antibiotique est réalisé à l’arrivée des poussins, décaler les traitements du circuit d’eau à deux jours après la fin de l’antibiotique.If antibiotic treatment is performed at the arrival of the chicks, shift the treatments of the water circuit to two days after the end of the antibiotic.
-
- RésultatsResults
- Les figures 4 à 10 représentent les résultats obtenus pour la boiterie, la mortalité, le traitement avec les antibiotiques, les tarses brûlés chez les mâles, et la consommation d’aliments.Figures 4 to 10 show the results obtained for lameness, mortality, treatment with antibiotics, tarsi burned in males, and food consumption.
- Les figures 4 et 5 montrent une diminution significative du nombre d’animaux ayant des tarses brûlés chez les mâles (figure 4) ou les femelles (figure 5) lors du triple traitement avec la composition selon l’invention, comparé à l’échantillon témoin. Figures 4 and 5 show a significant decrease in the number of animals having tarsi burned in males ( Figure 4 ) or females ( Figure 5 ) during the triple treatment with the composition according to the invention, compared to the control sample .
- Les figures 6 et 7 montrent que les animaux traités avec la composition selon l’invention selon les trois modes d’application ont significativement besoin de moins d’antibiotiques. On observe une tendance de 12 ppm/kg de poids vif d’antibiotique administrés de moins dans les lots essais par rapport aux témoins, soit une baisse de 39% de la consommation d’antibiotiques. Figures 6 and 7 show that animals treated with the composition according to the invention according to the three modes of application significantly less need antibiotics. There was a trend of 12 ppm / kg live weight of antibiotic administered less in the test lots compared to controls, a 39% decrease in antibiotic consumption.
- Les mâles ont un poids plus important après traitement avec la composition selon l’invention (figure 8), tout comme les femelles avant le 1er abattage (figure 9).Males have a greater weight after treatment with the composition according to the invention (Figure 8), as females before 1 slaughter (Figure 9).
- Enfin la mortalité est diminuée chez les animaux ayant été traités avec les compositions selon l’invention comparés aux animaux témoins (figure 10)Finally, the mortality is reduced in the animals that have been treated with the compositions according to the invention compared to the control animals ( FIG. 10 ).
- En conclusion, on observe un bénéfice significatif sur la santé des animaux, et leur bien être lors de l’utilisation simultanée de la composition dans l’environnement, une application sur l’animal et une ingestion par eau de boisson.In conclusion, there is a significant benefit to the health of animals, and their welfare during the simultaneous use of the composition in the environment, an application on the animal and ingestion by drinking water.
- EXEMPLE 5 – Application dans l’environnement, sur l’animal et par inhalation : PorcsEXAMPLE 5 - Application in the environment, on the animal and by inhalation: pigs
- Cet essai a pour objectif de montrer si une application combinée de la composition décrite à l’exemple 1 dans l’environnement, sur l’animal et par inhalation est efficace. Donc vérifier par rapport à l’exemple précédant, si d’autres types d’applications peuvent être réalisés comme l’inhalation à la place de l’ingestion.This test aims to show whether a combined application of the composition described in Example 1 in the environment, on the animal and by inhalation is effective. So check against the previous example, if other types of applications can be made such as inhalation instead of ingestion.
- L’essai a été réalisé dans 2 élevages différents selon les protocoles suivants.The test was carried out in 2 different farms according to the following protocols.
- Elevage 1 :Breeding 1:
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Stade Stadium Evénement Event Traitement témoin (placebo) Control treatment (placebo) Traitement essai PS PS test treatment Traitement essai PS+ENG PS + ENG test treatment PS + ENG PS + ENG Vidange préfosse, nettoyage désinfection (ND) puis vide sanitaire (VS)Prefosse drainage, cleaning disinfection (ND) then crawl space (VS) Oui Yes Oui Yes Oui Yes PS PS Nb porcs/salle Number of pigs / room 180 180 180 180 180 180 Prlv* J0 Prlv * J0 Oui Yes Oui Yes Oui Yes Pulv* J0 Pulv * J0 Placebo Placebo 2 doses/1000 m² 2 doses / 1000 m² 2 doses/1000 m² 2 doses / 1000 m² Pulv J15 Pulv J15 Placebo Placebo 1 dose/1000 m²1 dose / 1000 m² 1 dose/1000 m²1 dose / 1000 m² Pulv J30 Pulv J30 Placebo Placebo 1 dose/1000 m²1 dose / 1000 m² 1 dose/1000 m²1 dose / 1000 m² Prlv J42 Prlv J42 Oui Yes OuiYes OuiYes ENGENG Prlv J0 Prlv J0 Oui Yes Oui Yes Oui Yes Pulv J0 Pulv J0 Placebo Placebo PlaceboPlacebo 1 dose/1000 m²1 dose / 1000 m² Pulv J15 Pulv J15 Placebo Placebo PlaceboPlacebo 1 dose/1000 m²1 dose / 1000 m² Pulv J45 Pulv J45 Placebo Placebo PlaceboPlacebo 1 dose/1000 m²1 dose / 1000 m² Prlv J50 Prlv J50 Oui Yes Oui Yes Oui Yes Pulv J75 Pulv J75 Placebo Placebo PlaceboPlacebo 1 dose/1000 m²1 dose / 1000 m² Prlv J90 Prlv J90 Oui Yes Oui Yes Oui Yes - *Prlv = prélèvements bactériologiques, Pulv = Nébulisation, ENG : engraissement, PS : post sevrage* Prlv = bacteriological samples, Pulv = Nebulization, ENG: fattening, PS: post weaning
- Elevage 2 :Breeding 2:
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Stade Stadium Evénement Event Traitement témoin (placebo) Control treatment (placebo) Traitement essai Treatment test PS+EngPS + Eng PS + ENG PS + ENG Vidange préfosse, ND puis VSDrain prefosse, ND then VS Oui Yes Oui Yes PS PS Nb porcs/salle Number of pigs / room 350 350 350 350 Prlv* J0 Prlv * J0 Oui Yes Oui Yes Pulv* J0 Pulv * J0 Placebo Placebo 2 doses/1000 m² 2 doses / 1000 m² Pulv J15 Pulv J15 Placebo Placebo 1 dose/1000 m²1 dose / 1000 m² Pulv J30 Pulv J30 Placebo Placebo 1 dose/1000 m²1 dose / 1000 m² Prlv J42 Prlv J42 Oui Yes OuiYes ENGENG Prlv J0 Prlv J0 Oui Yes Oui Yes Pulv J0 Pulv J0 Placebo Placebo 1 dose/1000 m²1 dose / 1000 m² Pulv J15 Pulv J15 Placebo Placebo 1 dose/1000 m²1 dose / 1000 m² Pulv J45 Pulv J45 Placebo Placebo 1 dose/1000 m²1 dose / 1000 m² Prlv J50 Prlv J50 Oui Yes Oui Yes Pulv J75 Pulv J75 Placebo Placebo 1 dose/1000 m²1 dose / 1000 m² Prlv J90 Prlv J90 Oui Yes Oui Yes ABA ABA Abattage J100 (groins) Slaughter J100 (snouts) Oui Yes Oui Yes - *Prlv = prélèvements bactériologiques, Pulv = Nébulisation, ENG : engraissement, PS : post sevrage, ABA : Abattage* Prlv = bacteriological samples, Pulv = Nebulization, ENG: fattening, PS: post weaning, ABA: slaughter
- Dans cet exemple, sont comparés des animaux traités avec la composition selon l’invention selon les trois modes de traitement par rapport à des animaux n’ayant pas reçu la composition de l’invention (placebo).In this example, animals treated with the composition according to the invention are compared according to the three treatment modes with respect to animals that have not received the composition of the invention (placebo).
- L’application a été réalisée avec un nébulisateur en présence des animaux donc les flores ont été appliquées sur les surfaces des salles (E), sur les animaux (A) et en inhalation (I).The application was carried out with a nebulizer in the presence of the animals so the flora was applied to the surfaces of the rooms (E), animals (A) and inhalation (I).
- Des porcs pris aléatoirement dans l’élevage 2 ont été analysés après l’abattage en mesurant les déformations sur des coupes de groins.Pigs randomly taken from Livestock 2 were analyzed after slaughter by measuring deformities on slit sections.
- Différents prélèvements bactériologiques ont été réalisés avant la première application de flore dans les différentes salles pour évaluer la qualité de l’étape de nettoyage et désinfection et à la sortie des animaux des salles après les différentes applications de flores pour évaluer la pression pathogène.Various bacteriological samples were taken before the first application of flora in the different rooms to evaluate the quality of the cleaning and disinfection step and the exit of the animals from the rooms after the different applications of flora to evaluate the pathogenic pressure.
- Résultats :Results:
- Les résultats sont compilés dans le tableau suivant : The results are compiled in the following table:
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Notation nez (coupe de groin en abattoir)Nose rating (cut of groin in slaughterhouse) Elevage 2Breeding 2 Placebo n=20 PCPlacebo n = 20 PC Flore n=19 PCFlora n = 19 PC Note 0Rating 0 66 1212 Note 1Note 1 1010 77 Note 2Note 2 33 00 Note 3Note 3 11 00 Note 4Note 4 00 00 MoyenneAverage 0,950.95 0,370.37 TB état nasalTB nasal condition 30%30% 63%63% - TB état nasal : très bon état nasalTB nasal condition: very good nasal condition
- Note 0 : absence de déviation de la cloison et d’atrophie des volutes et cornetsNote 0: absence of wall deviation and atrophy of scrolls and horns
- Note 4 : déviation de la cloison et atrophie des volutes et cornetsNote 4: Partition deviation and atrophy of scrolls and horns
- L’échantillon placebo (sans composition) indique 30% des individus avec un très bon état anatomique nasal (absence de déviation de la cloison nasale, absence d’atrophie des volutes et cornets nasaux). The placebo sample (without composition) indicates 30% of the individuals with a very good anatomical nasal state (absence of deviation of the nasal septum, absence of atrophy of the scrolls and nasal turbinates).
- L’échantillon essai (porcs élevés en présence de flore de barrière depuis l’entrée en post-sevrage jusqu’en fin d’élevage) révèle que 2 fois plus (63%) d'individus enregistrent un très bon état anatomique nasal vs placebo.The test sample (pigs bred in the presence of barrier flora from post-weaning to the end of breeding) reveals that twice as many (63%) of individuals have a very good anatomic state of the nasal vs placebo .
- Il apparait que l’application selon l’invention apporte un effet significatif sur la santé et le bien-être des animaux.It appears that the application according to the invention has a significant effect on the health and well-being of animals.
- Les résultats bactériologiques montrent que plus la phase de nettoyage et désinfection des salles a été bien réalisée, plus la flore de barrière a montré de meilleurs résultats sur l’inhibition de la flore pathogène :The bacteriological results show that the better the cleaning and disinfection phase of the rooms, the more barrier flora showed better results on the inhibition of the pathogenic flora:
-
Note de la qualité de la phase de nettoyage désinfection (1=excellente à 4=absence)Note of the quality of the disinfection cleaning phase (1 = excellent to 4 = absence) Note 1,3Note 1,3 Note 1,9Note 1.9 Note 2,2Note 2.2 Note 2,4Rating 2.4 Fréquence de meilleur résultat de la flore de barrière sur la flore pathogène vs placebo, en fin de périodeFrequency of best result of barrier flora on pathogenic flora vs placebo, end of period 5/6 (83%)5/6 (83%) 4/6 (67%)4/6 (67%) 3/6 (50%)3/6 (50%) 0/6 (0%)0/6 (0%) - Conclusionsconclusions
- Cet essai montre aussi l’importance de l’application environnement dont l’efficacité est améliorée lorsque le nettoyage et désinfection des surfaces en amont de l’application est bien réalisé. L’intérêt du produit se mesure tant sur les animaux (sanitaire), que vis-à-vis de l’éleveur (conditions de travail).This test also shows the importance of the application environment whose efficiency is improved when the cleaning and disinfection of the surfaces upstream of the application is well done. The interest of the product is measured both on the animals (sanitary), and vis-à-vis the breeder (working conditions).
- EXEMPLE 6 – Application dans l’environnement et sur l’animal en comparaison avec application environnement, animal et ingéré : PorcsEXAMPLE 6 - Application in the environment and on the animal in comparison with application environment, animal and ingested: pigs
- Cet essai a pour but de confirmer l’intérêt de la combinaison des trois modes d’applications de la composition décrite à l’exemple 1 par rapport à la combinaison de seulement deux d’entre eux.This test is intended to confirm the interest of the combination of the three modes of application of the composition described in Example 1 compared to the combination of only two of them.
- L’élevage suivi est un élevage de 700 truies naisseur engraisseur en sélection multiplication (5 bandes de 120 truies). L’essai s’est déroulé sur 2 bandes à l’intérieur desquelles 3 lots de 40 truies ont été constitués en respectant une répartition homogène des rangs de portée. Le protocole d’application comprend la pulvérisation d’une flore de barrière dans l’environnement, sur les mamelles et le périnée des truies ainsi qu’une administration probiotique aux truies via la machine à soupe. The breeding followed is a breeding of 700 sows fattener breeder selection (5 strips of 120 sows). The trial was carried out on 2 bands in which 3 batches of 40 sows were formed, with a uniform distribution of the litter ranks. The application protocol includes spraying a barrier flora into the environment, on the udders and perineum of sows, and probiotic administration to sows via the soup machine.
- Protocoles de pulvérisation appliqués :Spray protocols applied:
-
Lot 1Lot 1 Lot 2Lot 2 Lot 3Lot 3 Lot 4Lot 4 BandeGang 1 + 2 1 + 2 11 1 + 21 + 2 22 Antibiotique sur porceletsAntibiotic on piglets ouiYes ouiYes ouiYes nonno J-3J-3 Environnementet truies Environment and sows Environnementet truies Environment and sows Environnementet truies Environment and sows Environnementet truies Environment and sows J-2J-2 J-1J-1 Mamelles et périnée Mamelles and perineum Mamelles et périnée Mamelles and perineum Mamelles et périnée Mamelles and perineum Mamelles et périnée Mamelles and perineum J0 : Mise basJ0: Farrowing J1J1 Mamelles et périnée Mamelles and perineum Mamelles et périnée Mamelles and perineum Mamelles et périnée Mamelles and perineum Mamelles et périnée Mamelles and perineum J4J4 Mamelles et périnéeMamelles and perineum Mamelles et périnéeMamelles and perineum Equivalence protocoleProtocol equivalence A + EA + E A + EA + E A + EA + E A + E + IA + E + I - L’ensemble des truies a reçu une dose de probiotiques à l’auge de J-3 à J1 par rapport à la mise bas.All sows received a dose of probiotics at J-3 to D1 from baseline.
- Lors de la seconde bande, l’ensemble des truies ont reçu un shampoing avant l’arrivée en maternité, contrairement à la première bande.In the second band, all sows received shampoo before arrival in the maternity ward, unlike the first band.
- Seuls les porcelets du lot 4 n’ont pas reçu d’antibiotique au moment des soins. L’antibiotique utilisé pour l’ensemble des autres lots étant très efficace contre les flores positives, les inventeurs considèrent dans cet essai que le protocole de ces lots équivaut à une application uniquement environnement et sur l’animal car les flores ingérées par les porcelets n’ont pas survécu à l’antibiotique.Only piglets in Lot 4 did not receive an antibiotic at the time of care. Since the antibiotic used for all the other batches is very effective against positive floras, the inventors consider in this test that the protocol of these batches is equivalent to an environment-only application and to the animal because the flora ingested by the piglets have not survived the antibiotic.
- Les inventeurs s’intéressent donc ici aux différences observées entre les lots 1 à 3 (A + E) avec le lot 4 (A + E + I).The inventors are therefore interested here in the differences observed between lots 1 to 3 (A + E) with lot 4 (A + E + I).
- Résultats :Results:
- Les figures 11 et 12 montrent qu’avec les trois applications combinées le risque d’apparition de diarrhée est moins important et le risque de mortalité également par rapport à une application uniquement environnement et sur l’animal. Figures 11 and 12 show that with the three applications combined the risk of developing diarrhea is less important and the risk of mortality also compared to an environment-only and animal-only application.
- La figure 13 montre que plus de cases comportant des porcelets dont les mères ont reçu un shampoing préalablement à l’application de flores sont saines d’un point de vue digestif en comparaison aux cases de porcelets dont les truies n’ont pas reçu de shampoing. En effet, dans le premier cas (bande 2) on observe au plus bas 78 cases sur 120 avec des fèces normales alors que dans le second cas (bande 1), seulement 50 cases sur 120. Figure 13 shows that more boxes with piglets whose mothers received a shampoo before the application of flora are healthy from a digestive point of view compared to the boxes of piglets whose sows have not received shampoo . Indeed, in the first case (band 2) one observes at the lowest 78 cases out of 120 with normal feces whereas in the second case (band 1), only 50 boxes out of 120.
- Ce résultat montre donc également l’importance de l’application sur l’animal, favorisée par le shampoing des truies pour un meilleur développement des flores positives, pour augmenter l’efficacité du produit. This result also shows the importance of the application on the animal, favored by shampooing sows for better development of positive flora, to increase the effectiveness of the product.
- Conclusions conclusions
- Dans cet exemple, les inventeurs ont montré que l’application A+E+I est plus efficace que l’application A+E. De plus, ils ont montré que si on favorise l’implantation des flores sur l’animal (application A mieux réalisée), on améliore la prévention des diarrhées.In this example, the inventors have shown that the application A + E + I is more effective than the application A + E. In addition, they have shown that if we promote the establishment of flora on the animal (A better application), we improve the prevention of diarrhea.
- L'invention n'est pas limitée aux modes de réalisation présentés et d'autres modes de réalisation apparaîtront clairement à l'homme du métier. The invention is not limited to the embodiments presented and other embodiments will become apparent to those skilled in the art.
Claims (10)
- Composition bactérienne comprenant au moins une souche de bactérie du genre Bacillus, notamment de Bacillus subtilis et au moins une souche de bactérie lactique, notamment au moins une souche de Lactococcus lactis, pour son utilisation dans le cadre de la prévention ou du traitement des infections pathogènes affectant la santé des animaux d’élevage terrestres,
ladite composition étant :
a- Ingérée ou inhalée par lesdits animaux d’élevage terrestres, et
b- soit
a1. épandue dans le milieu d’élevage desdits animaux d’élevage terrestres,
a2. appliquée sur lesdits animaux d’élevage terrestres,
a3. épandue dans le milieu d’élevage desdits animaux d’élevage terrestres et appliquée sur lesdits animaux d’élevage terrestres.Bacterial composition comprising at least one bacterium strain of the genus Bacillus, in particular Bacillus subtilis and at least one strain of lactic acid bacterium, in particular at least one Lactococcus strain lactis , for use in the prevention or treatment of pathogenic infections affecting the health of terrestrial livestock,
said composition being:
a- Ingested or inhaled by said terrestrial livestock, and
b- either
a1. spread in the breeding environment of said terrestrial livestock,
a2. applied to said terrestrial livestock,
a3. spread in the breeding environment of said terrestrial livestock and applied to said terrestrial livestock. - Composition pour son utilisation selon la revendication 1, dans laquelle la composition bactérienne comprend :
- au moins l'une des trois souches de Bacillus subtilis suivantes : NOL01, NOL02, NOL03, lesdites souches étant respectivement déposées à la CNCM sous le numéro CNCM I - 4606, CNCM I-5043 et CNCM I - 4607, et
- au moins la souche de bactérie lactique: Lactococcus lactis spp lactis 1 souche NOL11, ladite souche étant déposée à la CNCM sous le numéro CNCM I - 4609.The composition for use according to claim 1, wherein the bacterial composition comprises:
at least one of the following three Bacillus subtilis strains: NOL01, NOL02, NOL03, said strains being respectively deposited at the CNCM under the number CNCM I-4606, CNCM I-5043 and CNCM I-4607, and
- at least the strain of lactic acid bacteria: Lactococcus lactis spp lactis 1 strain NOL11, said strain being deposited at the CNCM under number CNCM I - 4609. - Composition pour son utilisation selon la revendication 1 ou la revendication 2, ladite composition comprenant de 104 à 1011 colonies bactériennes de Bacillus et de 104 à 1011 colonies bactériennes de bactéries lactiques les colonies bactériennes étant en gramme ou en millilitre de composition.A composition for use as claimed in claim 1 or claim 2, said composition comprising from 104 to 1011 bacterial colonies of Bacillus and from 104 to 1011 bacterial colonies of lactic acid bacteria, wherein the bacterial colonies are in grams or milliliters of composition.
- Composition pour son utilisation selon l’une quelconque des revendications 1 à 3, où ladite au moins une souche de Bacillus est sous forme sporulée et/ou sous forme végétative.A composition for use as claimed in any one of claims 1 to 3 wherein said at least one strain of Bacillus is in spore form and / or vegetative form.
- Composition pour son utilisation selon l’une quelconque des revendications 1 à 4, où ladite composition inhalée ou ingérée et ladite composition épandue dans le milieu d’élevage desdits animaux d’élevage terrestres et/ou appliquée sur lesdits animaux d’élevage terrestres, sont utilisées simultanément, séparément ou étalée dans le temps.A composition for use as claimed in any one of claims 1 to 4, wherein said inhaled or ingested composition and said composition spread in the culture medium of said terrestrial livestock and / or applied to said terrestrial livestock are used simultaneously, separately or spread over time.
- Composition pour son utilisation selon l’une quelconque des revendications 1 à 5, où ladite composition est épandue et/ou appliquée préalablement à l’ingestion ou l’inhalation par lesdits animaux d’élevage terrestres.A composition for use as claimed in any one of claims 1 to 5, wherein said composition is spread and / or applied prior to ingestion or inhalation by said terrestrial livestock.
- Composition pour son utilisation selon l’une quelconque des revendications 1 à 6, dans laquelle ladite composition consiste essentiellement en les quatre souches bactériennes du groupe constitué par les trois souches Bacillus subtilis NOL01, NOL02, NOL03, lesdites souches étant respectivement déposées à la CNCM sous le numéro CNCM I - 4606, CNCM I-5043 et CNCM I – 4607 et la souche Lactococcus lactis spp lactis 1 souche NOL11, ladite souche étant déposée à la CNCM sous le numéro CNCM I – 4609.A composition for its use according to any one of claims 1 to 6, wherein said composition consists essentially of the four bacterial strains of the group consisting of the three strains Bacillus subtilis NOL01, NOL02, NOL03, said strains being respectively deposited at the CNCM under the CNCM number I-4606, CNCM I-5043 and CNCM I-4607 and the Lactococcus lactis spp lactis strain, strain NOL11, said strain being deposited at the CNCM under number CNCM I-4609.
- Composition pour son utilisation selon l’une quelconque des revendications 1 à 7, où ladite composition est épandue par m2 à raison de 105 à 1011 colonies bactériennes.A composition for use as claimed in any one of claims 1 to 7, wherein said composition is spread per m2 at 105 to 1011 bacterial colonies.
- Composition pour son utilisation selon l’une quelconque des revendications 1 à 8, où l’environnement est nettoyé et désinfecté et les animaux terrestres sont lavés préalablement à l’épandage dans le milieu d’élevage desdits animaux d’élevage terrestres et/ou l’application sur lesdits animaux d’élevage terrestres.A composition for use according to any one of claims 1 to 8, wherein the environment is cleaned and disinfected and the terrestrial animals are washed prior to application in the rearing medium of said terrestrial livestock and / or application to said terrestrial livestock.
- Trousse de prévention des infections pathogènes affectant la santé et le bien-être des animaux d’élevage terrestre comprenant
- une composition telle que définie dans l’une quelconque des revendications précédentes, et
- au moins un agent de biosécurité et/ou au moins un agent de biocontrôle.Prevention Kit for Pathogenic Infections Affecting the Health and Well-Being of Terrestrial Livestock Including
a composition as defined in any one of the preceding claims, and
at least one biosecurity agent and / or at least one biocontrol agent.
Applications Claiming Priority (2)
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FR1760495A FR3073139B1 (en) | 2017-11-08 | 2017-11-08 | COMPOSITION IMPROVING ANIMAL WELFARE |
PCT/EP2018/080666 WO2019092134A1 (en) | 2017-11-08 | 2018-11-08 | Composition for improving the well-being of animals |
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EP3675885A1 true EP3675885A1 (en) | 2020-07-08 |
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EP18807884.4A Withdrawn EP3675885A1 (en) | 2017-11-08 | 2018-11-08 | Composition for improving the well-being of animals |
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US (1) | US20200345794A1 (en) |
EP (1) | EP3675885A1 (en) |
FR (1) | FR3073139B1 (en) |
WO (1) | WO2019092134A1 (en) |
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FR3105730B1 (en) * | 2019-12-31 | 2022-08-12 | Nolivade | Composition for the treatment of mammalian pathologies |
FR3106725B1 (en) * | 2020-01-31 | 2022-08-05 | Nolivade | Composition of microorganisms for the breeding of insects |
FR3106724B1 (en) * | 2020-01-31 | 2022-08-05 | Nolivade | Composition for improving insect production |
FR3110344B1 (en) * | 2020-05-20 | 2023-12-15 | Nolivade | Decontamination process |
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FR2758051B1 (en) | 1997-01-06 | 1999-12-17 | Cobiotex | METHODS FOR SYSTEMATICALLY ERADICATING THE CARRYING OF PATHOGENS BY ANIMALS AND COMPOSITIONS USED THEREIN |
FR2991182B1 (en) * | 2012-05-30 | 2014-05-30 | Nolivade | BACTERIAL COMPOSITION FOR THE TREATMENT OF COLIBACILLOSIS IN LIVESTOCK, IN PARTICULAR AVIAN FARMS AND DRINKING WATER COMPRISING SUCH A BACTERIAL COMPOSITION |
WO2014151837A1 (en) * | 2013-03-15 | 2014-09-25 | Bayer Cropscience Lp | A bacillus subtilis strain for use in maintaining or improving the paw health of poultry |
RU2546880C2 (en) * | 2013-06-03 | 2015-04-10 | Федеральное государственное бюджетное учреждение науки Институт проблем экологии и эволюции им. А.Н. Северцова Академии Наук | Method of probiotic feed preparation for farm animals |
CN103773721A (en) | 2013-12-31 | 2014-05-07 | 陕西万源生物农业科技有限公司 | Composite probiotics for raising pigs |
CN104307012B (en) | 2014-11-05 | 2017-08-11 | 双胞胎(集团)股份有限公司 | A kind of special deodorant of livestock and poultry farm and its application |
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2017
- 2017-11-08 FR FR1760495A patent/FR3073139B1/en active Active
-
2018
- 2018-11-08 WO PCT/EP2018/080666 patent/WO2019092134A1/en unknown
- 2018-11-08 EP EP18807884.4A patent/EP3675885A1/en not_active Withdrawn
- 2018-11-08 US US16/762,260 patent/US20200345794A1/en not_active Abandoned
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FR3073139A1 (en) | 2019-05-10 |
US20200345794A1 (en) | 2020-11-05 |
WO2019092134A1 (en) | 2019-05-16 |
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