EP3458195A1 - Embout de pipette pour centrifugeuse comprenant deux fermetures - Google Patents

Embout de pipette pour centrifugeuse comprenant deux fermetures

Info

Publication number
EP3458195A1
EP3458195A1 EP13713260.1A EP13713260A EP3458195A1 EP 3458195 A1 EP3458195 A1 EP 3458195A1 EP 13713260 A EP13713260 A EP 13713260A EP 3458195 A1 EP3458195 A1 EP 3458195A1
Authority
EP
European Patent Office
Prior art keywords
pipette tip
liquid
fitting
centrifuge
laboratory
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP13713260.1A
Other languages
German (de)
English (en)
Inventor
Joel OLIVEIRA CORREIA VASCO
Hugo Miguel TOMÉ LOURENÇO MARQUES
Fábio Alexandre MARQUES GUEDES
Dina Margarida PEDRO MATOS TEIXEIRA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Instituto Politecnico De Leiria
Original Assignee
Instituto Politecnico De Leiria
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instituto Politecnico De Leiria filed Critical Instituto Politecnico De Leiria
Publication of EP3458195A1 publication Critical patent/EP3458195A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021—Test tubes specially adapted for centrifugation purposes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • B01L3/50825—Closing or opening means, corks, bungs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/043—Hinged closures

Definitions

  • the scope of the present invention (1) has its focus on the reduction of laboratory steps, reducing processing time, minimizing errors, decreasing laboratory materials consumption and, therefore, minimizing the costs on laboratory procedures where the currently available pipette tips and centrifuge tubes are normally used.
  • the optimization of the pipetting and storage process is aimed by combining the functions of these two laboratory consumables through the design of an innovative pipette tip (2) and its tight lid (9) .
  • the reduction of the drawbacks listed before are obvious, particularly, observing the present invention (1) ability of pipetting a determined liquid volume and to ensure tightness of the content by fitting the correspondent lids (6) and (9) .
  • the present invention (1) stores the liquid content in biological security conditions, maintaining its physical and chemical integrity, enabling future centrifugation and analysis.
  • the storage of the dosed liquid volume can be performed using Eppendorf type test tubes (US5354539, US5254314, US2007/0031297 ) and fluid discharge from the pipette tip to the centrifuge tube is achieved by interrupting the vacuum realized by the pipettor, consequently, the fluid content is ejected to the centrifuge tube. The centrifuge tube is then closed and can proceed to the centrifugation process.
  • Eppendorf type test tubes US5354539, US5254314, US2007/0031297
  • the document CN201164593 Y refers to a pipette tip combined with a centrifuge tube whose main purpose is to prepare platelet rich plasma and its main difference consists on an upper lid with a multifunctional hole that seals simultaneously the centrifuge tube and the pipette tip.
  • JP2010038876 A refers to a centrifuge tube which main feature is to have an inside step to enable the pipetting of a lower density fluid, since after the centrifugation procedure, the higher density fluid remains at the bottom of the centrifuge tube.
  • the present invention (1) differs from the others by having a tight lid (9) and a annular snap-fit (8) that, after fitting, ensure the sealing of the lower hole (4) . Therefore, the present invention (1) shows innovation by combining the functions of a pipette tip and a centrifuge tube, enabling that dosing and storage procedures can be made on the same unit of volume. Thus, the centrifugation process is performed ensuring liquid content tightness, preventing liquid loss and liquid contamination from the outside .
  • FIG 1 Schematics of invention (1), which besides pipetting, enables storage for future liquid content analysis, by fitting the lids (6) and (9) on the correspondent upper (3) and lower holes (4) .
  • the pipette tip (2) has an upper lid (6) which is connected to the pipette tip (2) through the integral hinge (5), enabling the articulation of the upper lid (6) and the fitting of the upper socket (7) on the upper hole (3) .
  • the geometry of the upper socket (7) ensures liquid content tightness, although it enables successive opening and closing operations.
  • the annular snap-fit (8) on the bottom of the pipette tip (2) enables the fitting of tight lid (9), fully closing the system.
  • the lower hole (4) has the diameter D5 suitable for a precise and effective pipetting process.
  • FIG. 2 Section view of the tight pipette tip for centrifuge (1), depicting the inside and outside diameters on the pipette tip (2), respectively Dl and D2.
  • the diameter Dl was designed to fit on the bays of a centrifuge and the diameter D2 was designed to be linked on a pipettor .
  • Figure 3 Schematic representation of the fitting between the body of the pipette tip (2) and the tight lid (9) .
  • Figure 4 Section view of detail A of Figure 3, showing the fitting between the pipette tip (2) and the tight lid
  • FIG. 9 This detail illustrates the tight lid (9) assembled on the annular snap-fit (8), whose geometries are designed to ensure full volume sealing by applying the required effort.
  • the depth HI of the tight lid (9) should be higher than the height H2 of the annular snap-fit (8) and the diameter D3 of the annular snap-fit (8) should be lower than the inside diameter D4 of the tight lid (9) to enable assembly.
  • the diameter D6 of the conical pin (11) should be higher than the diameter D5 of the lower hole (4) to ensure liquid tightness
  • Figure5 Schematical representation of the assembly of the tight lid (9) on the pipette tip (2) and the closing of the upper hole (3) through the upper lid (6) .
  • This representation also shows the useful length H3 and the maximum length H4 of the invention (1).
  • the useful length H3 is suitable for the depth of the centrifuge bays.
  • Figure 6 Schematical representation of the pipetting process using the present invention (1) .
  • Figure 6a Represents the pipette tip (2) fitted on a generical pipettor, sucking a fluid through the lower hole (4) from a generic container.
  • Figure 6b Represents the pipette tip (2) with the liquid content dosed within, with the tight lid (9) being placed while the pipettor maintains the vacuum.
  • Figure 6c Represents the invention (1), showing the lower hole (4) closed by the tight lid (9) and the upper hole (3) closed by the upper lid (6) .
  • Figure 6d Illustrates the invention (1) with the fluid content dosed within, placed On a bay of a generic centrifuge .
  • the present invention (1) refers to a tight pipette tip for a laboratory centrifuge enabling both pipetting and storage functions, resulting from the combination of the features of two laboratory products currently available, the pipette tip and the centrifuge tube.
  • the present invention (1) has the ability of pipetting the desired liquid volume and after fitting the tight lid (9) through the annular snap-fit (8) on the lower hole (4) and fitting the upper socket (7) on the upper hole (3) through the upper lid (6) which is connected to the centrifuge tip (2) through the integral hinge (5), can be stored or analyzed, maintaining its physical and chemical integrity as well as liquid tightness. Laboratory procedures can then be simplified with the present invention (1) since the pipetted liquid content, storage and analysis can be performed on the same unit of volume. DETAILED DESCRIPTION OF THE INVENTION
  • the present invention (1) refers on a pipette tip (2) and a tight lid (9) for pipetting and storage functions, resulting from the combination of the features of two laboratory products currently available, the pipette tip and the centrifuge tube.
  • These laboratory consumables, pipette tip and the centrifuge tube are individually and concomitant used worldwide in research and educational laboratories to perform tests and analysis in domains such as chemistry, molecular biology and biochemistry.
  • the present invention (1) is focused on the optimization of processes where pipette tips and centrifuge tubes are normally used, by reducing or even eliminating the drawbacks caused by the use of both devices.
  • the use of the present invention (1) avoids contamination resulting from pipetting and storage operations in different volume units, dosing inaccuracy due to the residual fluid volume that remains on the pipette tip, expenditure of consumables that are discarded necessarily after use, minimization of operator errors and shortening operation time.
  • the present invention intends to simplify the laboratorial process of pipetting, storage and analysis fluids in the same volume unit.
  • the present invention (1) consists on a pipette tip (2) and a tight lid (9) .
  • the pipette tip (2) has, on its top side, an upper lid (6) that is connected through an integral hinge (5), which enables the articulation of the tight lid (6) and the fitting of the upper socket (7) on the upper hole (3) .
  • the upper hole (3) diameter is suitable to fit the pipettor, enabling vacuum pipetting to be efficient.
  • the geometry of the upper socket (7) ensures liquid content tightness, although it enables successive opening and closing operations.
  • the sealing of the pipetted liquid content is ensured by fitting the tight lid (9), subsequent removal of the pipettor and adjustment of the upper lid (6) , which is connected to the pipette tip (2) through the integral hinge (5) .
  • Close to the bottom of the pipette tip (2) there is the annular snap- fit (8) which enables the fitting of the tight lid (9) by applying the required effort, considering that the geometries of the annular snap-fit (8) and tight lid (9) are designed to ensure full volume sealing, since once fitted the lower hole (4) is permanently closed.
  • the manufacturing process to be adopted for the invention (1) is injection moulding of thermoplastics, since it is the mass production technology that enables the processing of a suitable raw material and to reduce the correspondent manufacturing costs;
  • the raw material adopted for the invention (1) could be polypropylene (homopolymer or copolymer) or polydxymethylene, since these are the raw materials that enable the use of the integral hinge (5) .
  • the following dimensional characteristics of the invention (1) are provided only by way of example and are not intended to limit it in any way: c. the diameter D5 may vary between 0,25 and 1.2 mm depending on the viscosity of the fluid to be pipetted;
  • the diameter Dl may vary between 5.85 and 19.28 mm depending on the diameter of the bay of the centrifuge;
  • the diameter D2 may vary between 4.37 and 17.78 mm depending on the size of the pipettor
  • the length H3 can have up to 43 mm depending on the depth of the bay of the centrifuge, considering that this length can be enlarged to increase the volume capacity of the invention (1) .

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Centrifugal Separators (AREA)

Abstract

La présente invention (1) se rapporte à un embout de pipette étanche pour une centrifugeuse de laboratoire permettant à la fois des fonctions de pipetage et de stockage, résultant de la combinaison des caractéristiques de deux produits de laboratoire disponibles actuellement, l'embout de pipette et le tube centrifuge. Les embouts de pipette actuels ont deux trous, un trou supérieur pour permettre l'adaptation sur une pipette et le trou inférieur pour permettre l'entrée du liquide aspiré sous l'effet du vide par la pipette. Le liquide peut ensuite être déposé sur un tube centrifuge pour être centrifugé. La présente invention (1) combine ces deux consommables de laboratoire en un dispositif unique et, par conséquent, a la capacité de pipeter le volume de liquide souhaité et après adaptation du couvercle étanche (9) par adaptation à encliquetage annulaire (8) sur le trou inférieur (4) et adaptation de la douille supérieure (7) sur le trou supérieur (3) par l'intermédiaire du couvercle supérieur (6) qui est relié à l'embout de pipette (2) par l'articulation intégrée (5), peut être stocké ou analysé, conservant son intégrité physique et chimique ainsi que l'étanchéité au liquide. Les procédures de laboratoire peuvent alors être simplifiées avec la présente invention (1) étant donné que le contenu de liquide pipeté, le stockage et l'analyse peuvent être réalisés sur la même unité de volume.
EP13713260.1A 2012-03-12 2013-02-19 Embout de pipette pour centrifugeuse comprenant deux fermetures Withdrawn EP3458195A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PT106204A PT106204A (pt) 2012-03-12 2012-03-12 Ponta de pipetadora estanque para centrífuga
PCT/PT2013/000010 WO2013137756A1 (fr) 2012-03-12 2013-02-19 Embout de pipette pour centrifugeuse comprenant deux fermetures

Publications (1)

Publication Number Publication Date
EP3458195A1 true EP3458195A1 (fr) 2019-03-27

Family

ID=48040398

Family Applications (1)

Application Number Title Priority Date Filing Date
EP13713260.1A Withdrawn EP3458195A1 (fr) 2012-03-12 2013-02-19 Embout de pipette pour centrifugeuse comprenant deux fermetures

Country Status (3)

Country Link
EP (1) EP3458195A1 (fr)
PT (1) PT106204A (fr)
WO (1) WO2013137756A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10730043B2 (en) * 2015-03-17 2020-08-04 Micro Blood Science Inc. Sample collection and separation device
JP6104440B1 (ja) * 2016-08-17 2017-03-29 株式会社エム・ビー・エス 血液採取器及び血液収容器を含む血液採取器具

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4748859A (en) 1987-03-06 1988-06-07 Rainin Instrument Co., Inc. Disposable pipette tip
DE3843610A1 (de) * 1988-01-13 1989-07-27 Stephan Dr Diekmann Trenn- oder reaktionssaeuleneinheit
US5254314A (en) 1989-08-24 1993-10-19 International Mould Engineering Microcentrifuge tube
CA2105962A1 (fr) * 1992-09-18 1994-03-19 Margaret Patricia Raybuck Dispositif et procede de separation d'affinite
US5249711A (en) * 1992-10-01 1993-10-05 Du Pont Canada Inc. Disposable dispensing pipette
US5354539A (en) 1993-11-12 1994-10-11 Hovatter Kenneth R Microtube having press-to-seal and twist-to-lock closure cap
AU2034999A (en) * 1997-08-01 1999-02-22 Aalto Scientific, Ltd. Pipetting devices preloaded with standardized control sample materials
DE19917375C2 (de) 1999-04-16 2001-09-27 Hamilton Bonaduz Ag Bonaduz Pipettiereinheit
US7320259B2 (en) 2004-03-06 2008-01-22 Medax International, Inc. Pipette tip for easy separation
FR2889461B1 (fr) 2005-08-04 2007-10-26 Gilson Sas Soc Par Actions Sim Microtube de laboratoire.
US20080078258A1 (en) 2006-09-28 2008-04-03 Price West L Multi-component pipette tip and associated methods
CN201164593Y (zh) * 2008-03-16 2008-12-17 毕建萍 离心管与吸液管
JP5153511B2 (ja) * 2008-08-08 2013-02-27 株式会社エンプラス チューブ及び遠心分離装置
WO2010081107A2 (fr) 2009-01-12 2010-07-15 Biotix, Inc. Extrémités de pipette flexibles

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
None *
See also references of WO2013137756A1 *

Also Published As

Publication number Publication date
PT106204A (pt) 2013-09-12
WO2013137756A1 (fr) 2013-09-19

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