EP3430126A4 - An ex-vivo intestinal culture model, methods of producing same and uses thereof - Google Patents

An ex-vivo intestinal culture model, methods of producing same and uses thereof Download PDF

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Publication number
EP3430126A4
EP3430126A4 EP17765995.0A EP17765995A EP3430126A4 EP 3430126 A4 EP3430126 A4 EP 3430126A4 EP 17765995 A EP17765995 A EP 17765995A EP 3430126 A4 EP3430126 A4 EP 3430126A4
Authority
EP
European Patent Office
Prior art keywords
methods
producing same
culture model
vivo intestinal
intestinal culture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP17765995.0A
Other languages
German (de)
French (fr)
Other versions
EP3430126A1 (en
Inventor
Hinanit KOLTAI
Yoram Kapulnik
Marcelo FRIDLENDER
Timna NAFTALI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mor Research Applications Ltd
Israel Ministry of Agriculture and Rural Development
Agricultural Research Organization of Israel Ministry of Agriculture
Original Assignee
Mor Research Applications Ltd
Israel Ministry of Agriculture and Rural Development
Agricultural Research Organization of Israel Ministry of Agriculture
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mor Research Applications Ltd, Israel Ministry of Agriculture and Rural Development, Agricultural Research Organization of Israel Ministry of Agriculture filed Critical Mor Research Applications Ltd
Publication of EP3430126A1 publication Critical patent/EP3430126A1/en
Publication of EP3430126A4 publication Critical patent/EP3430126A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/76—Undefined extracts from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP17765995.0A 2016-03-17 2017-03-17 An ex-vivo intestinal culture model, methods of producing same and uses thereof Withdrawn EP3430126A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662309603P 2016-03-17 2016-03-17
US201662358601P 2016-07-06 2016-07-06
PCT/IL2017/050338 WO2017158609A1 (en) 2016-03-17 2017-03-17 An ex-vivo intestinal culture model, methods of producing same and uses thereof

Publications (2)

Publication Number Publication Date
EP3430126A1 EP3430126A1 (en) 2019-01-23
EP3430126A4 true EP3430126A4 (en) 2019-10-30

Family

ID=59850270

Family Applications (1)

Application Number Title Priority Date Filing Date
EP17765995.0A Withdrawn EP3430126A4 (en) 2016-03-17 2017-03-17 An ex-vivo intestinal culture model, methods of producing same and uses thereof

Country Status (5)

Country Link
US (1) US20190100731A1 (en)
EP (1) EP3430126A4 (en)
CA (1) CA3017823A1 (en)
IL (1) IL261847A (en)
WO (1) WO2017158609A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013164824A1 (en) 2012-05-03 2013-11-07 Magdent Ltd. Bone enhancement device and method
EP3277260B1 (en) 2015-04-01 2021-05-12 The State of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organization (ARO) (Volcani Center) Erodium crassifolium l'her plant extracts and uses thereof
CN110621312A (en) 2017-03-05 2019-12-27 以色列国家农业部、农村发展农业研究组织·沃尔卡尼中心 Compositions and methods for treating cancer
US20190293634A1 (en) * 2018-02-28 2019-09-26 Albert Li Isolated intestinal mucosa and uses thereof
EP4193154A1 (en) * 2020-08-07 2023-06-14 Athos Therapeutics, Inc. Ex vivo gastrointestinal biopsy platform to evaluate multi-omic signatures for screening candidate therapeutics

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040175367A1 (en) * 2001-08-23 2004-09-09 Meenhard Herlyn Organotypic intestinal culture and methods of use thereof
US20100047853A1 (en) * 2008-08-21 2010-02-25 Calvin Jay Kuo Ex Vivo Culture, Proliferation and Expansion of Intestinal Epithelium
US20100286098A1 (en) * 2007-07-05 2010-11-11 Gw Pharma Limited Use of tetrahydrocannabinol and/or cannabidiol for the treatment of inflammatory bowel disease

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040175367A1 (en) * 2001-08-23 2004-09-09 Meenhard Herlyn Organotypic intestinal culture and methods of use thereof
US20100286098A1 (en) * 2007-07-05 2010-11-11 Gw Pharma Limited Use of tetrahydrocannabinol and/or cannabidiol for the treatment of inflammatory bowel disease
US20100047853A1 (en) * 2008-08-21 2010-02-25 Calvin Jay Kuo Ex Vivo Culture, Proliferation and Expansion of Intestinal Epithelium

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
AMANDA L. KAUFFMAN ET AL: "Alternative functional in vitro models of human intestinal epithelia", FRONTIERS IN PHARMACOLOGY, vol. 4, no. 79, 1 January 2013 (2013-01-01), pages 1 - 18, XP055503086, DOI: 10.3389/fphar.2013.00079 *
B.S. HARVEY ET AL: "Interleukin 17A evoked mucosal damage is attenuated by cannabidiol and anandamide in a human colonic explant model", CYTOKINE, vol. 65, no. 2, 13 November 2013 (2013-11-13), US, pages 236 - 244, XP055602795, ISSN: 1043-4666, DOI: 10.1016/j.cyto.2013.10.006 *
EVANS G S ET AL: "THE DEVELOPMENTS OF A METHOD FOR THE PREPARATION OF RAT INTESTINAL EPITHELIAL CELL PRIMARY CULTURES", JOURNAL OF CELL SCIENCE,, vol. 101, no. 1, 1 January 1992 (1992-01-01), pages 219 - 231, XP009006507, ISSN: 0021-9533 *
LOUIS-PHILIPPE PAGEOT ET AL: "Human cell models to study small intestinal functions: Recapitulation of the crypt-villus axis", MICROSCOPY RESEARCH AND TECHNIQUE., vol. 49, no. 4, 15 May 2000 (2000-05-15), GB, pages 394 - 406, XP055622386, ISSN: 1059-910X, DOI: 10.1002/(SICI)1097-0029(20000515)49:4<394::AID-JEMT8>3.0.CO;2-K *
See also references of WO2017158609A1 *
TOSHIRO SATO ET AL: "Long-term Expansion of Epithelial Organoids From Human Colon, Adenoma, Adenocarcinoma, and Barrett's Epithelium", GASTROENTEROLOGY : OFFICIAL PUBLICATION OF THE AMERICAN GASTROENTEROLOGICAL ASSOCIATION, WILLIAMS & WILKINS, US, vol. 141, no. 5, 27 July 2011 (2011-07-27), pages 1762 - 1772, XP028325676, ISSN: 0016-5085, [retrieved on 20110902], DOI: 10.1053/J.GASTRO.2011.07.050 *

Also Published As

Publication number Publication date
WO2017158609A1 (en) 2017-09-21
IL261847A (en) 2018-10-31
CA3017823A1 (en) 2017-09-21
US20190100731A1 (en) 2019-04-04
EP3430126A1 (en) 2019-01-23

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