EP3356041B1 - Dispositif et procédé de stockage et de vidage de contenants remplis de fluide dans des dispositifs microfluidiques - Google Patents

Dispositif et procédé de stockage et de vidage de contenants remplis de fluide dans des dispositifs microfluidiques Download PDF

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Publication number
EP3356041B1
EP3356041B1 EP16760753.0A EP16760753A EP3356041B1 EP 3356041 B1 EP3356041 B1 EP 3356041B1 EP 16760753 A EP16760753 A EP 16760753A EP 3356041 B1 EP3356041 B1 EP 3356041B1
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EP
European Patent Office
Prior art keywords
container
hollow needle
actuator
receiving unit
receiving
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP16760753.0A
Other languages
German (de)
English (en)
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EP3356041A1 (fr
Inventor
Thomas BRETTSCHNEIDER
Daniel Czurratis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Robert Bosch GmbH
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Robert Bosch GmbH
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Filing date
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Publication of EP3356041A1 publication Critical patent/EP3356041A1/fr
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0672—Integrated piercing tool
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • B01L2400/0683—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers mechanically breaking a wall or membrane within a channel or chamber
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent
    • B01L3/523—Containers specially adapted for storing or dispensing a reagent with means for closing or opening

Definitions

  • Lab-on-a-chip systems are microfluidic devices in which several functionalities of a macroscopic laboratory are accommodated on a plastic substrate, for example, the size of a credit card, and miniaturized and at least partially automated to run complex biological, diagnostic, chemical or physical processes.
  • the liquids and reagents required for this can be introduced into a cartridge of the lab-on-a-chip system during operation or can already be stored on the cartridge.
  • microfluidic cartridges are known, in which a foil bag, also called a tubular bag, filled with fluid is stored in front.
  • a device for receiving and fluidly contacting a container for a fluid.
  • the device comprises a receiving unit for receiving a container, the receiving unit having at least one first hollow needle and at least one fixing element for fixing the received container at a predetermined distance from the first hollow needle.
  • the first hollow needle extends in the direction of the received container.
  • the fixing element is designed such that when the force exerted on the container in the direction of the first hollow needle or on the receiving unit in the direction of the container exceeds a predetermined threshold value, the fixing of the container with the fixing element is released and thereby a predetermined movement of the receiving unit and the container relative to each other is made possible in such a way that the first hollow needle penetrates an envelope of the container for fluid contacting of the container.
  • a microfluidic device can in particular be understood to mean a microfluidic chip, a microfluidic cartridge or a lab-on-a-chip system.
  • a container for a fluid can in particular be understood to mean a blister or a tubular bag.
  • the container can be at least partially filled with a fluid, for example with liquids or reagents, for processes to be carried out in the device.
  • a hollow needle can in particular be understood to mean a needle which at least partially has a channel, preferably along a section of the longitudinal axis of the needle, the channel having at least at one end an opening to an outer region around the needle.
  • the device has the advantage that the liquids or reagents required for operation of the device, which are generally designed for long-term stable storage, can be accommodated in the device together with their respective container and can be stored long-term stable until use.
  • the container it is not necessary to provide the device itself with special materials which are characterized by adequate barrier properties for long-term stable storage of the fluids or reagents.
  • the device according to the invention can also be introduced into a microfluidic device shortly before processing.
  • the device can preferably also comprise a plurality of holding units for holding containers, so that a plurality of containers with fluids or reagents necessary for the operation of the device can be held in the device.
  • the at least one fixing element comprises a latching lug for latching in part of the casing of the accommodated container.
  • the threshold value of the force exerted for a solution of the fixation of the container in the locking lug can be predetermined by designing a width and / or a depth of the locking lug.
  • the locking lug can be designed, for example, as a depression or recess in the fixing element or in a surface of the receiving unit.
  • the latching lug can be designed, for example, as a depression or recess in a curvature protruding from the fixing element or from a surface of the receiving unit, for example a bead-shaped elevation.
  • Shaping at least one fixing element as a latching lug is particularly advantageous when a part of the casing of the container to be received protrudes from the container in relation to an environment of the casing, since this makes it easier to snap into the latching lug of the part.
  • This is particularly the case with blister packs which comprise a barrier or sealing film, with at least a part, in particular an end section, of the sealing or barrier film projecting beyond the rest of the blister casing and thus being particularly suitable for latching into the latching lug suitable.
  • the receiving unit comprises a second hollow needle, which extends in the direction of the container and which is arranged such that the second hollow needle, when the receiving unit and the container move relative to one another, the sleeve of the container for introducing a fluid pushed into the container.
  • a fluidic contacting of the container via the second hollow needle enables a particularly effective emptying of the container, since a fluid introduced into the container via the second hollow needle can displace the fluid upstream in the container from the container into the device via the first hollow needle.
  • the fluid introduced into the container via the second hollow needle can be a gas, for example nitrogen, a gas mixture, for example air, or a liquid.
  • a liquid that does not mix with the fluid in the container can preferably be used.
  • the most complete emptying of the container can be achieved by introducing a fluid through the second hollow needle.
  • the receiving unit has a first surface and a second surface and third surface connected to the first surface.
  • the second surface and the third surface are each connected to the first surface in such a way that the surfaces delimit a receiving space for the container in a U-shaped manner, the first and / or the second hollow needle extending from the first surface into the receiving space.
  • This has the advantage that a container to be accommodated can be fixed in a simple manner via one or more fixing elements arranged on the second surface and / or third surface.
  • the second surface and the third surface are preferably designed in such a way that the receiving space tapers in the direction of the first surface.
  • Such a taper advantageously has the effect that a movement of a received container in the direction of the first and / or second hollow needle counteracts an increasing resistance as soon as the received container touches the second and the third surface.
  • the sealing or barrier film is thereby put under tension, which facilitates penetration of the first or the second hollow needle into the container.
  • the second surface has a first fixing element and the third surface has a second fixing element for fixing the accommodated container at the predetermined distance from the first hollow needle, the length of the shortest distance between the first fixing element and the second fixing element is between 0.3 cm and 5 cm, preferably between 0.5 cm and 1 cm. Since blisters are available in various standard sizes, in particular in a standard size, the width of the sealing or barrier film being 0.5-1.5 cm, this development has the advantage that a sealing or barrier film of a blister of the standard size a receptacle of the blister and a fixation by the two fixing elements undergoes a bend and is under tension, which facilitates penetration of the first and / or second hollow needle into the blister.
  • the first and / or the second hollow needle comprise a sealing ring arranged around the first and / or second hollow needle.
  • a device in particular a microfluidic device, with a device and an actuator is described, at least part of the actuator being movably arranged with respect to the device.
  • the actuator is arranged in such a way that when a force is exerted on a part of the actuator in the direction of the device or on the device in the direction of the actuator when the force is exerted, the force exerted is passed on via the part of the actuator to a container accommodated in the receiving unit of the device fixation of the container with the fixing element is released and the predetermined movement of the receiving unit and the container relative to one another takes place via a predetermined movement of the part of the actuator and the device relative to one another.
  • the actuator comprises an elastic layer or a flexible membrane which can be pressurized via a channel of the actuator or the device.
  • an elastic layer or flexible membrane has the advantage that a transmission of force via the elastic layer to the accommodated container can take place in a particularly uniform manner due to the flexibility of the layer or membrane and over a large contact area between layer or membrane and container, so that the transmitted force or pressure is distributed over a larger area of the container. This reduces the risk of undesired destruction of the casing in the contact area between the actuator and the container.
  • the part of the actuator comprises at least one plunger and / or a plunger-shaped and / or plunger-shaped shape.
  • the actuator is arranged such that when the force is exerted, at least part of the force acts on a predetermined part of the casing of the container by touching the plunger and / or the plunger-shaped shape and / or the plunger-shaped shape.
  • the actuator can be arranged such that the part of the force acts on a protruding part of the casing of the container.
  • Such a part of the actuator has the advantage that the force is transferred to the container via a predefined point.
  • a force transmission can take place via a force effect of the plunger on a projecting part of the casing of the blister and another part of the casing, in particular the part that delimits the fluid Part of the envelope that can be kept free from the actuator.
  • the device comprises an insert element that is arranged between the accommodated container and the actuator for transmitting force to the container.
  • the insert element preferably comprising a plunger or stamp-shaped shape, a force exerted by the actuator on the insert element can be transferred in a targeted manner to defined locations of the accommodated container.
  • the device comprises a frame element, the frame element framing at least a part of the receiving element and at least a part of the frame element being shaped such that the part of the frame element prevents removal of a received container that exceeds a predetermined size.
  • the shape of the part of the frame element determines a minimum size for a container to be accommodated in the receiving unit.
  • the elastic layer of the actuator is partially connected to the receiving element and arranged between the receiving element and the frame element in such a way that if the elastic layer extends beyond a predetermined extent in the direction of the frame element, the receiving element in the direction of the received container for a fluid Contacting the container is moved.
  • the frame element and the receiving element can thus be moved relative to one another.
  • the accommodated container is advantageously moved through the part of the frame element in the direction of the first and / or second hollow needle.
  • the frame element can preferably have a U-shape, a space formed by the U-shape being provided for receiving the receiving element.
  • the shape of the part of the frame element is preferably in the form of at least one hook, the hook preferably being arranged with respect to a container of larger size in such a way that when the hooks move apart it contacts a protruding part of the casing of the container and, in the event of a continued movement apart, that of the container Movement underlying force at least partially transmits in the direction of the first and / or second hollow needle to the part of the sheath.
  • a channel for pressurizing the elastic layer is preferably arranged between the receiving element and the elastic layer, for example in the form of a recess in the receiving element.
  • This channel can advantageously produce an overpressure, for example by introducing a fluid into the channel, in order to cause the elastic layer to expand in the direction of the frame element.
  • the invention also relates to a system comprising a device according to the invention and a container accommodated in the receiving unit of the devices, the container being at least partially filled with a fluid.
  • the invention also relates to a method for receiving and emptying a container. In a first step, the container is received in a receiving unit with at least one first hollow needle and fixed at a predetermined spacing from the first hollow needle by at least one fixing element of the receiving unit.
  • a force is exerted on the container in the direction of the first hollow needle or on the receiving unit in the direction of the container, the exerted force exceeding a predetermined threshold value for releasing the fixation of the container.
  • the container and the receiving unit are moved towards one another, so that the first hollow needle penetrates an envelope of the container for fluid contacting of the container.
  • Figures 1a to 1c show an embodiment of the device 100 for receiving and fluidly contacting a container 10 for a fluid.
  • the container 10 is a blister with a sheath 11, the blister 10 being partially filled with a liquid 15 and the sheath 11 of the blister 10 comprising a barrier film 12.
  • the liquid 15 can preferably be removed by piercing or tearing open the barrier film 12.
  • the blister can have a volume between 100 and 10000 microliters, for example.
  • the sleeve 11 and in particular the barrier film 12 can be polymer composites with, for example, polypropylene (PP), polyethylene (PE), cyclo-olefin polymer (COP), cyclo-olefin copolymer (COC), polymer-metal composites with in particular polyethylene terephthalate (PET) / aluminum (AI) / PE, PET / AI / PP, AI / PP, AI / PE or other packaging materials, which represent a diffusion barrier for the liquid 15 contained in the container 10 and a long-term stable storage of the Allow liquid.
  • the barrier film 12 can have a thickness between 5 and 1000 micrometers.
  • the barrier film 12 preferably also has a barrier layer 13 with an exemplary thickness of 5 to 500 micrometers, which comprises, for example, vapor-deposited aluminum, polychlorotrifluoroethylene, stretched and / or transversely stretched polypropylene film or ethylene-vinyl alcohol copolymer (EVOH).
  • a barrier layer 13 with an exemplary thickness of 5 to 500 micrometers, which comprises, for example, vapor-deposited aluminum, polychlorotrifluoroethylene, stretched and / or transversely stretched polypropylene film or ethylene-vinyl alcohol copolymer (EVOH).
  • the device 100 can for example be part of a lab-on-a-chip, a microfluidic chip or a microfluidic cartridge.
  • the device 10 comprises a receiving unit 200 for receiving the container 10, the receiving unit 200 in this example comprising a first hollow needle 211 and a second hollow needle 212.
  • the two hollow needles 211, 212 extend from a first surface 201 of the receiving unit 200.
  • the receiving unit 200 further comprises a second surface 202 and a third surface 203 such that the surfaces 201, 202, 203 have a receiving space 220 for the received container 10 U -shaped, the hollow needles 211, 212 partially extend into the receiving space 220.
  • the receiving unit 200 can in particular comprise a polymer substrate, for example a thermoplastic such as PC, PP, PE, PMMA, COP, COC, and have a thickness of, for example, 0.5 to 5 mm.
  • the receiving unit 200 can be manufactured by milling, injection molding, hot stamping, deep drawing or laser structuring.
  • the second side 202 and the third side 203 of the receiving unit 200 comprise a first fixing element 221 and a second fixing element 222, which in this exemplary embodiment are designed in the form of locking lugs.
  • a width of the first side 201 is made smaller than a width of the barrier film 12 of the container 10 to be accommodated, so that projecting ends of the barrier film 12 can snap into the two latching noses 221, 222 and thereby the container received at a predetermined distance 250 from the first side 201 and thus is fixed by the two hollow needles 211, 212.
  • the locking lugs 221, 222 can be designed as depressions or recesses in the second side 202 and the third side 203.
  • the detents 221, 222 can also be designed as depressions or recesses in bulges protruding from the sides 202, 203, for example bead-shaped elevations. This enables the protruding ends of the barrier film 12 to be clipped or wedged into the latches 221, 222, as shown in the sequence of figures 1a, 1b, 1c.
  • the predetermined spacing 250 of the received and fixed container 10 in the receiving unit 200 is chosen so that damage to the barrier film 12 by the spaced hollow needles 211, 212 in the event of usual vibrations during storage and transport operations is prevented.
  • a spacing 250 of 100 ⁇ m to 2 mm can be selected.
  • the first hollow needle 211 and the second hollow needle 212 comprise a sealing ring 215, 216 arranged around the respective hollow needle, in order to prevent the fluid from flowing outside the hollow needles when the barrier film 12 of the container 10 is pierced.
  • the barrier film 12 can have an elastic layer 13 with an exemplary thickness of 5 to 500 micrometers, the elasticity of the layer causing an additional sealing effect around the hollow needles 211, 212.
  • the elastic layer 13 can comprise a rubber, an elastomer, a thermoplastic elastomer and / or a silicone.
  • Figure 1c shows the device 100 with the container 10 as the system 1000 of this exemplary embodiment.
  • Figures 2a to 2d show in a development of the embodiment Figure 1 an embodiment of the device 500 according to the invention, which device 100 according to the embodiment Figure 1 and includes an actuator 300.
  • the actuator 300 in this exemplary embodiment comprises two L-shaped parts 310, each with a plunger-shaped shape 320 of the respectively longer section of the L-shape, the parts 310 being movably arranged relative to the device 100.
  • the L-shape of the parts 310 has the advantage that the force on the parts 310 exerts a force on the projecting ends of the barrier film 12 of the Container 10 can be forwarded.
  • the actuator could, for example, also have a shape partially enclosing the container 10, for example a U shape.
  • the container 10 is moved further in the direction of the projecting hollow needles 211, 212 via the moving parts of the actuator 310, these pierce the barrier film 12 of the container 10 and fluidic contact is established between the device 100 and the container 10, as in FIG Figure 2b shown.
  • a fluid 15 located in the container can then be removed from the container 10 via the first hollow needle 211 and / or the second hollow needle 212.
  • the liquid 15 can advantageously be removed from the container 10 via the lower of the two hollow needles 211, 212 with respect to the gravitational field of the earth.
  • the first hollow needle 211 is the lower of the two hollow needles 211 and 212, via which liquid 15 is withdrawn.
  • the device can comprise a pump 110 connected to the first hollow needle 211.
  • a particularly efficient emptying process 10 is achieved if, as indicated in the sequence of figures 1c and 1d, a replacement fluid 16, for example a gas or gas mixture, is introduced into the container via the second hollow needle 212 in order to displace the liquid 15 from the container 10 to be supported by the first hollow needle 211.
  • the introduction of the replacement fluid can take place here via a second pump 120 of the device, the second pump 120 being fluidly coupled to the second hollow needle 212 and being able to comprise a reservoir for the replacement fluid.
  • the second hollow needle 212 can be connected to an environment around the device 100 or around the device 500, so that when sucking through the first Pump 110 on the first hollow needle 211, air from the environment flows into the container 10 as a replacement fluid via the second hollow needle 212.
  • the movable parts 310 of the actuator 300 are preferably designed such that when the container 10 is fluidly contacted with the device 100, the movable parts 310 are positively contacted with the receiving unit 200, so that further force is exerted on the actuator via the receiving unit 200 is forwarded and the container 10 experiences no further force.
  • Such form-fitting contacting can take place, for example, through the L-shaped design of the moving parts 310 of the actuator 300.
  • the hollow needles can comprise metal.
  • Figures 3a to 3c show an alternative development according to the invention of the embodiment Figure 1
  • the device 500 according to the invention turns off the device 100 Figure 1 and an actuator 300 with a flexible membrane in the form of an elastic layer 330.
  • the membrane 330 can be pressurized via a channel 350 of the actuator 300, so that it expands in the direction of the container 10 accommodated in the receiving unit 200 of the device 100 and transmits the pressure to the container 10 as a force.
  • Figure 3b It is shown that, by exerting force on the container 10 through the membrane 330, the container 10 has been released from a fixation with the locking lugs 221, 222 and moved in the direction of the hollow needles 211, 212, so that the hollow needles 211, 212 have the barrier film 12 pierce the container 10. As in Figure 3c shown, the container 10 can then be emptied, for example via the first hollow needle 211.
  • Figures 4a and 4b show a development of the embodiment Figure 3 , an insert 400 being arranged between the film 330 of the actuator 300 and the container 10 accommodated in the receiving unit 200.
  • the insert 400 can comprise the same materials as the receiving element 200.
  • the insert 400 has the advantage that one of the membrane 330 transferred force through the insert part 400 is not transmitted to the entire envelope 11 of the container 10, but only to projecting ends 14 of the barrier film 12. This reduces the risk that the container 10 will deform and the envelope 11 of the container 10 will tear due to an overpressure in the container 10.
  • the device 500 according to the invention comprises a device 100, an actuator 300 with an elastic layer 330 and a frame element 450.
  • the frame element 450 frames at least a part of the receiving element 200.
  • a part 451, 452 of the frame element 450 is shaped such that the part 451, 452 of the frame element 450 prevents the removal of a received container 10, for example a blister, which exceeds a predetermined size.
  • the frame element 450 can comprise the same materials as the receiving element 200.
  • the elastic layer 330 of the actuator 300 is partially connected to the receiving element 200 and arranged between the receiving element 200 and the frame element 450 in such a way that if the elastic layer 330 extends beyond a predefined extent in the direction of the frame element 450, the receiving element 200 in the direction of the Received container 10 is moved for fluidic contacting of the container 10, as indicated in the sequence of figures 5a to 5b.
  • the frame element 450 and the receiving element 200 can thus be moved relative to one another.
  • the accommodated container 10 is advantageously moved through the part 451, 452 of the frame element 450 in the direction of the first and second hollow needles 211, 212.
  • the first and second hollow needles 211, 212 are connected to a first channel 213 and to a second channel 214, respectively, the channels 213, 214 in Figure 5 protrude from the image plane.
  • the membrane 330 can be at least partially arranged between the receiving element 200 and a substrate layer 340.
  • the shape of the part 451, 452 of the frame element 450 is in the form of a first hook 451 and a second hook 452, the hooks 451, 452 preferably being arranged with respect to a blister 10 that exceeds the size, in such a way that the Moving the hooks apart contacts a projecting part of the sheath 11, namely in each case a projecting end 14 of the barrier film 12 of the blister 10 and, in the event of a continued movement apart, at least partially transmits the force underlying the movement apart in the direction of the first and second hollow needles 211, 212 to the ends 14 ,
  • a channel 210 for pressurizing the elastic is preferably arranged between the receiving element 200 and the elastic layer 330, for example in the form of a recess 210 in the receiving element 200.
  • an excess pressure can be caused by this channel 210, for example by introducing a fluid into the channel 210, are generated in order to cause the elastic layer 330 to expand in the direction of the frame element 450 for fluidic contacting of the blister 10, as from Figure 5b seen.
  • Figure 6 shows a flowchart of an exemplary embodiment of the method 800 according to the invention.
  • a container is received in a receiving unit with at least one first hollow needle and fixed at a predetermined spacing from the first hollow needle by at least one fixing element of the receiving unit.
  • a force is exerted on the container in the direction of the first hollow needle or on the receiving unit in the direction of the first container, the exerted force exceeding a predetermined threshold value for releasing the fixation of the container.
  • the container and the receiving unit are moved towards one another in such a way that the first hollow needles penetrate a casing of the container for fluid contacting of the container.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Feeding, Discharge, Calcimining, Fusing, And Gas-Generation Devices (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Medical Preparation Storing Or Oral Administration Devices (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Claims (12)

  1. Appareil (500), en particulier appareil microfluidique (500), comportant un dispositif (100), en particulier un dispositif microfluidique (100), pour la réception et la mise en contact fluidique d'un contenant (10) pour un fluide, le dispositif (100) comportant une unité de réception (200) pour la réception du contenant (10), l'unité de réception (200) comprenant au moins une première aiguille creuse (211) et au moins un élément de fixation (220) pour une fixation du contenant reçu (10) à une distance prédéfinie (250) de la première aiguille creuse (211), la première aiguille creuse (211) s'étendant en direction du contenant reçu (10) et l'élément de fixation (220) étant réalisé de telle sorte que, lorsqu'une application de force sur le contenant (10) en direction de la première aiguille creuse (211) ou sur l'unité de réception (200) en direction du contenant (10) dépasse une valeur seuil prédéfinie, la fixation du contenant (10) à l'aide de l'élément de fixation (220) est relâchée et un déplacement prédéfini de l'unité de réception (200) et du contenant (10) l'un par rapport à l'autre est ainsi rendu possible, de telle sorte que la première aiguille creuse (211) pénètre une enveloppe (11) du contenant (10) pour une mise en contact fluidique du contenant (10), l'appareil (500) comportant un actionneur (300), au moins une partie (310) de l'actionneur (300) étant conçue de manière mobile par rapport au dispositif (100), l'actionneur (300) étant disposé de telle sorte que, lorsqu'une application de force sur la partie (310) de l'actionneur (300) en direction du dispositif (100) ou sur le dispositif (100) en direction de l'actionneur (300) dépasse une valeur seuil prédéfinie, une fixation du contenant (10) à l'aide de l'élément de fixation (220, 221, 222) est relâchée par une transmission de l'application de force, par le biais de la partie (310) de l'actionneur (300), à un contenant (10) reçu dans l'unité de réception (200) du dispositif (100) et le déplacement prédéfini de l'unité de réception (200) et du contenant (10) l'un par rapport à l'autre s'effectue par le biais d'un déplacement prédéfini de la partie (310) de l'actionneur (300) et du dispositif (100) l'un par rapport à l'autre, caractérisé en ce que la partie (310) de l'actionneur (300) comporte une couche élastique ou une membrane souple (330), laquelle peut être soumise à une pression par le biais d'un canal (350) de l'actionneur (300) ou du dispositif (100).
  2. Appareil (500) selon la revendication 1, dans lequel ledit au moins un élément de fixation (220) comporte un ergot d'encliquetage (221, 222) pour l'encliquetage d'une partie de l'enveloppe (11) du contenant reçu (10).
  3. Appareil (500) selon l'une des revendications précédentes, dans lequel l'unité de réception (200) comporte une deuxième aiguille creuse (212), laquelle s'étend en direction du contenant (10) et laquelle est disposée de telle sorte que la deuxième aiguille creuse (212), lors du déplacement prédéfini de l'unité de réception (200) et du contenant (10) l'un par rapport à l'autre, pénètre l'enveloppe (11) du contenant (10) pour une introduction d'un fluide dans le contenant (10).
  4. Appareil (500) selon l'une des revendications précédentes, dans lequel l'unité de réception (200) comprend une première surface (201) st respectivement une deuxième surface (202) et une troisième surface (203) reliées à la première surface (201) de telle sorte que les surfaces (201, 202, 203) délimitent, en forme de U, un espace de réception (220) pour le contenant (10), dans lequel la première et/ou la deuxième aiguille creuse (211, 212) s'étendent à partir de la première surface (201) dans l'espace de réception (220).
  5. Appareil (500) selon la revendication 4, dans lequel la deuxième surface (202) et la troisième surface (203) sont formées de telle sorte que l'espace de réception (220) se rétrécit en direction de la première surface (201) .
  6. Appareil (500) selon la revendication 4 ou 5, dans lequel la deuxième surface (202) comprend un premier élément de fixation (221) et la troisième surface (202) comprend un deuxième élément de fixation (222) pour la fixation du contenant reçu (10) à la distance prédéfinie (250) de la première aiguille creuse (211), et dans lequel une longueur de la plus courte distance entre le premier élément de fixation (221) et le deuxième élément de fixation (222) est comprise entre 0,3 cm et 5 cm, de préférence entre 0,5 cm et 1.
  7. Appareil (500) selon l'une des revendications précédentes, dans lequel la première et/ou la deuxième aiguille creuse (211, 212) comportent une bague d'étanchéité (215, 216) disposée autour de la première et/ou la deuxième aiguille creuse (211, 212).
  8. Appareil (500) selon l'une des revendications précédentes, dans lequel la partie (310) de l'actionneur (300) comporte au moins un poussoir et/ou une partie saillante (320) en forme de poussoir et/ou en forme de piston, dans lequel l'actionneur (300) est disposé de telle sorte que, lors de l'application de force, au moins une partie (310) de la force agit sur une partie prédéfinie de l'enveloppe (11) du contenant (10) par le biais d'un contact du poussoir (320), respectivement de la partie saillante en forme de poussoir ou en forme de piston.
  9. Appareil (500) selon l'une des revendications précédentes, dans lequel l'appareil (500) comporte en outre un élément d'insertion (400) qui est disposé entre le contenant reçu (10) et l'actionneur (300) pour une transmission de force au contenant (10).
  10. Appareil (500) selon l'une des revendications précédentes, dans lequel l'appareil (500) comporte un élément de cadre (450), dans lequel l'élément de cadre (450) encadre au moins une partie de l'élément de réception (200) et dans lequel au moins une partie (451, 452) de l'élément de cadre (450) est formée de telle sorte que la partie (451, 452) de l'élément de cadre (450) empêche un retrait d'un contenant reçu (10) dépassant une taille prédéfinie, et dans lequel la couche élastique (330) de l'actionneur (300) est reliée partiellement à l'élément de réception (200) et est disposée entre l'élément de réception (200) et l'élément de cadre (450) de telle sorte que dans le cas d'une étendue de la couche élastique (330) en direction de l'élément de cadre (450) qui dépasse une étendue prédéfinie, l'élément de réception (200) est déplacé en direction du contenant reçu (10) pour une mise en contact fluidique du contenant (10).
  11. Système comportant un appareil (500) selon l'une des revendications précédentes et un contenant (10) reçu dans l'unité de réception (200) du dispositif (100), dans lequel le contenant (10) est rempli au moins partiellement d'un fluide (15).
  12. Procédé (800) de réception et de vidage d'un contenant (10) dans un appareil (500) selon l'une des revendications 1 à 10, comportant les étapes de :
    • réception (801) du contenant (10) dans l'unité de réception (200) et fixation du contenant (10) suivant un espacement prédéfini (250) par rapport à la première aiguille creuse (211) au moyen d'au moins un élément de fixation (220, 221, 222) de l'unité de réception (200)
    • application (802) d'une force sur le contenant (10) en direction de la première aiguille creuse (211) ou sur l'unité de réception (200) en direction du contenant (10), la force appliquée dépassant la valeur seuil prédéfinie pour un relâchement de la fixation du contenant (10)
    • rapprochement (803) du contenant (10) et de l'unité de réception (200) de telle sorte que la première aiguille creuse (211) pénètre une enveloppe du contenant (10) pour une mise en contact fluidique du contenant (10) .
EP16760753.0A 2015-09-29 2016-09-07 Dispositif et procédé de stockage et de vidage de contenants remplis de fluide dans des dispositifs microfluidiques Active EP3356041B1 (fr)

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DE102015218665.2A DE102015218665A1 (de) 2015-09-29 2015-09-29 Vorrichtung und Verfahren zur Lagerung und Entleerung von mit Fluid gefüllten Behältern in mikrofluidischen Vorrichtungen
PCT/EP2016/071049 WO2017055039A1 (fr) 2015-09-29 2016-09-07 Dispositif et procédé de stockage et de vidage de contenants remplis de fluide dans des dispositifs microfluidiques

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CN111558404B (zh) * 2020-05-12 2022-05-10 南方科技大学 微流控芯片液滴路径规划方法、装置、设备及存储介质
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DE102022207608A1 (de) 2022-07-26 2024-02-01 Robert Bosch Gesellschaft mit beschränkter Haftung Fluidriegel für eine mikrofluidische Vorrichtung, Tank für eine mikrofluidische Vorrichtung, mikrofluidische Vorrichtung und Verfahren zum Betreiben einer mikrofluidischen Vorrichtung

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US20180272344A1 (en) 2018-09-27
WO2017055039A1 (fr) 2017-04-06
US11007522B2 (en) 2021-05-18
EP3356041A1 (fr) 2018-08-08
DE102015218665A1 (de) 2017-03-30

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