EP3170045A1 - Systeme de trajectographie automatique pour microscope optique base sur une camera rapide intelligente - Google Patents
Systeme de trajectographie automatique pour microscope optique base sur une camera rapide intelligenteInfo
- Publication number
- EP3170045A1 EP3170045A1 EP15741521.7A EP15741521A EP3170045A1 EP 3170045 A1 EP3170045 A1 EP 3170045A1 EP 15741521 A EP15741521 A EP 15741521A EP 3170045 A1 EP3170045 A1 EP 3170045A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- sample
- optical microscope
- optical
- tracking system
- focus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 230000003287 optical effect Effects 0.000 title claims abstract description 58
- 238000013507 mapping Methods 0.000 title abstract 2
- 230000010354 integration Effects 0.000 claims abstract description 19
- 238000013519 translation Methods 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 9
- 238000000399 optical microscopy Methods 0.000 claims description 7
- 238000012937 correction Methods 0.000 claims description 6
- 238000001514 detection method Methods 0.000 claims description 6
- 230000005284 excitation Effects 0.000 claims description 4
- 230000001360 synchronised effect Effects 0.000 claims description 3
- 238000004364 calculation method Methods 0.000 claims description 2
- 239000000523 sample Substances 0.000 description 59
- 238000003384 imaging method Methods 0.000 description 19
- 230000008901 benefit Effects 0.000 description 8
- 238000005070 sampling Methods 0.000 description 8
- 230000033001 locomotion Effects 0.000 description 7
- 230000005540 biological transmission Effects 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 241000446313 Lamella Species 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 230000003071 parasitic effect Effects 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 230000035945 sensitivity Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 230000002964 excitative effect Effects 0.000 description 3
- 238000000799 fluorescence microscopy Methods 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 238000001454 recorded image Methods 0.000 description 3
- 230000004044 response Effects 0.000 description 3
- 230000004907 flux Effects 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 230000008045 co-localization Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000002073 fluorescence micrograph Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000006916 protein interaction Effects 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000001429 visible spectrum Methods 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/241—Devices for focusing
- G02B21/244—Devices for focusing using image analysis techniques
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
Definitions
- the present invention is in the field of 3D tracking systems for optical microscopy.
- the present invention relates to the field of fluorescence microscopes.
- This type of microscope uses an excitatory source whose spectrum is adapted to the type of fluorescence sought and the type of sample.
- This source is usually an arc lamp, an Electro Luminescent Diode (LED) or a Laser.
- the exciter source illuminates all or part of the sample to be imaged which absorbs the corresponding photons and in turn emits photons at a different wavelength.
- This fluorescence is then collected by the microscope and detected by a photosensitive sensor.
- Fluorescence microscopes are generally also equipped with a second source, often of the white light type, making it possible to observe for the same sample an image by transmission contrast.
- a focus maintenance system is required in any type of automated microscope to compensate for these drift effects over time. This system thus makes it possible to benefit from a perfect focus on the sample regardless of the mechanical or thermal changes that occur at the level of the optical column of the microscope.
- the hardware methods are based on devices controlling the position of the coverslip by measuring a reflected optical signal.
- a first device is to reflect a semicircle of infrared light from an LED at 870 nm on the observed lamella and project this reflection on a linear CCD sensor to determine the position of the lamella.
- the signal thus generated at the CCD sensor is then used to slave the Z axis of the microscope.
- the accuracy obtained on the position of the focal plane is less than one third of the focal depth of the objective.
- the bandwidth of such a device is of the order of 200 Hz.
- a second device consists in projecting a grid onto the observed lamella, said lamella acting as a reflecting surface.
- the reflected image of this grid is then intercepted by a camera which is oriented in bias with respect to said reflected beam. In this way, only a small portion of the reflected grid is sharp on the camera.
- the relative position of the reflection of the grid on the camera changes.
- a servo system then compensates for this drift along the Z axis of the microscope until said reflection of the grid is clear again.
- the real-time correction algorithm used is generally very simple and does not include a state history in the correction made: the correction signal sent to the servo control system of the focal plane only depends on the current measurement without integrating the previous values. Nor is it predictive.
- the current known systems do not make it possible to control the position of the sample in the XY plane.
- a lateral drift of the microscope optical column or parasitic movement of the sample in one of the X or Y directions is neither measured nor compensated.
- the current systems perform only tasks of maintaining the object in a predetermined position thanks to an optical clamp.
- the position of the clamp may possibly be modified depending on the observation of the object by the microscope. But if the object moves or drifts at a speed too high compared to the refresh rate of the dynamic autofocus system (200 Hz) then said object can not be "caught" by the optical clamp.
- the object of the present invention is to respond at least in large part to the above problem and to furthermore to other advantages.
- An object of the invention is to provide a dynamic focusing system capable of detecting more sudden loss of focus of the sample.
- Another object of the invention is to propose a system for lateral control of the sample.
- Another object of the invention is to increase the bandwidth of the servo system in focus and / or in position.
- At least one of the aforementioned objectives is obtained with a tracking system for an optical microscope comprising a fast camera placed at the image plane of said optical system and able to detect an area of interest of said sample, a light source able to illuminate at least a portion of the sample and at least one control unit programmed to calculate spatial information of said area of interest of the sample.
- the fast camera integrates sequentially:
- the control unit can enslave at least the focus of said optical system and / or the position of the area of interest of the sample with the aid of the fast camera and said calculated spatial information. It is thus possible to circumvent the failures of previously known systems. It can record images of an area of interest in the sample - not a reflective surface of the coverslip - at a sufficient sampling rate and contrast level to enslave the microscope in focus and / or position.
- the fast camera can acquire between 2 and 20 times more images than a traditional camera and exploit them to enslave the microscope in focus and / or position.
- the present invention provides a device capable of acquiring images sufficiently detailed and fast enough to be used in a control loop of the translation stage which supports the sample.
- the present invention responds more accurately to the problem of control and of and guarantees the stability of the recorded image (in focus and in motion).
- the sampling frequency of said fast camera may be at least 100 Hz.
- said fast camera can image said sample in transmission.
- control unit can be integrated with the fast camera to control the focus and / or the position of said area of interest of the sample and to minimize the processing time. information recorded by said camera and - in fine - to increase the bandwidth of the servo system.
- said at least one control unit programmed to control the focus and / or the position of said area of interest of the sample may be a programmable network, a microprocessor, a graphics card or an ASIC to increase the bandwidth of the servo system and the reliability of said automatic imaging system.
- control unit and / or the fast camera can operate in real time.
- the time required to process the information necessary for the position and / or focus control is deterministic and the servocontrol of said microscope is more robust and more efficient.
- the invention may further comprise at least one optical system capable of focusing on a sample in order to automate these operations.
- the light source may be a pulsed LED source able to illuminate at least a portion of the sample and to increase the contrast on this area.
- the light source can be synchronized with said fast camera to improve the conditions of illumination of the sample and the contrast of the image used for the servocontrol, without disturbing any other mode imaging of the optical microscope used.
- the focus and the position of the sample can be adjusted during the acquisition of said at least a first series of images in order to perfect the focus and / or the position of the sample.
- the automatic imaging system for optical microscopy may further comprise an optical sensor, in particular capable of imaging the fluorescence phenomena.
- an optical sensor in addition to the fast camera, can dissociate the acquisition - fast - images used to enslave the microscope, those acquired for scientific purposes and for which the integration time is longer - not allowing such a servo control in focus and / or position.
- said fast camera and said optical sensor can operate in parallel: the acquisition of the fast camera is superimposed autonomously and independent of the scientific acquisition in order to easily and cheaply implement the present invention on existing devices. Furthermore, the bandwidth of the servo system is - in this case - increased.
- the sampling frequency of said fast camera can be at least ten times higher than that of said optical sensor in order to obtain the necessary sampling frequencies for a quality control (reliable and robust) .
- the spatial information used for the servocontrol in focus and / or in position of said sample may be of the type of detection of the maximum correlation with a reference image by said fast camera.
- This first similarity criterion is easily calculable and exploitable at high frequency to be then used in the servo loop to retroact on the translation stage and / or on the optical system.
- the spatial information used for the servocontrol in focus and / or position of said sample may be of the maximum contrast detection type by said fast camera.
- This second resemblance criterion is easily calculable and exploitable at high frequency to be then used in the servo loop to retroact on the translation stage and / or on the optical system.
- the optical microscope tracking system may further comprise an exciter light source capable of fluorescing said sample in order to highlight in particular certain physical and / or biological phenomena.
- the automatic imaging system for optical microscopy may further comprise at least one optical clip adapted to manipulate said sample.
- the automatic imaging system for optical microscopy may further comprise at least one method according to which the focus is corrected during the acquisition of the at least one a series of images recorded at high frequency by the fast camera and on the basis of which the control unit determines a spatial information used for the servocontrol of said focus.
- the invention comprises a video acquisition method of stabilized scientific images comprising the iterative steps (i) of recording a scientific first image with a first integration time, (ii) recording a series of high-frequency images, (iii) determination of a similarity criterion from the series of images, (iv) calculation of the corrective parameters in position and / or focus, (v) correction of the focus and / or position by said optical system and / or translation stage, (vi) recording of the next scientific image.
- the invention may also relate to the use of a tracking device for an optical microscope in order to perform 3D reconstruction of objects measured by said microscope.
- FIG. 1 schematically illustrates a microscope according to the present invention, in a configuration with a single photosensitive sensor
- FIG. 2 schematically illustrates a microscope according to the present invention, in a configuration with two photosensitive sensors
- Figure 3 schematically illustrates the servo system according to the present invention
- FIG. 4 illustrates a timing diagram according to a first embodiment of the present invention
- FIG. 5 illustrates a timing diagram according to a second embodiment of the present invention
- Figure 6 illustrates an embodiment of the present invention for focus control.
- variants of the invention comprising only a selection of characteristics subsequently described in isolation (even if this selection is isolated within a sentence comprising other characteristics), if this selection of characteristics is sufficient to confer a technical advantage or to differentiate the invention from the state of the prior art.
- This selection comprises at least one preferably functional characteristic without structural details, and / or with only a part of the structural details if this part alone is sufficient to confer a technical advantage or to differentiate the invention from the state of the art. earlier.
- Figure 1 schematizes a microscope 1 according to the invention.
- This is a non-limiting schematic view in which the image of a sample 9 placed between a microscope slide 6 and a coverslip 7 is amplified by a set of objectives 8.
- the sample is worn by a translation plate 17 to at least one axis and which can be motorized.
- the image thus obtained is formed on an optical sensor 12 which may be a camera or any other type of photosensitive sensor without restricting the claimed protection.
- the photosensitive sensor 12 is a fast camera.
- a fast camera is a camera that is capable of acquiring images at rates faster than conventional video streams (about 30 frames per second). Typically, we speak of a fast camera for frequencies at least greater than 100 images per second. As such, the fast camera is well known to those skilled in the art and is not, as such, the object of the present invention.
- an excitatory light source 3 completes the device. It is generally a broadband or narrowband laser source, whose wavelengths can be in the ultraviolet or in the wavelength range corresponding to the visible spectrum, ie between 200 and 600 nm.
- the exciting source 3 can take any shape and produce radiation of any type.
- the excitatory source is able to excite said sample and to make it fluoresce in response to this stimulus.
- the incident beam 14 is focused on the sample 9 using, for example, said objective set 8.
- the interaction zone 10 absorbs the incident and fluorescent light energy at a wavelength that is generally different. .
- This light emitted by the sample 9 in response to the excitation is picked up by the set of objectives 8 and sent back to the optical sensor 14 via, for example, a set of semi-reflecting optics 4, 5 and Reflecting mirror (s) 16.
- the images thus returned by this device, in response to a light excitation are representative of a physical and / or biological phenomenon of interest and are called "scientific images" by the following.
- the device 1 may be completed by a secondary illumination source 2 which illuminates all or part of the sample 9.
- This secondary light source 2 can illuminate the sample 9 in transmission as shown in FIG. other alternative mode - especially in reflection - without undermining the protection sought.
- the secondary light source can be of any type and produce a spectrum of light of any type, both broadband and in a so-called narrow band.
- the secondary light source may be a pulsed laser source, using an Electro Luminescent Diode for example, and at a wavelength greater than 800. nm so as not to interfere with the fluorescence phenomena and / or light interaction generated at the sample.
- the secondary light thus generated is captured in the same way as the exciting light 14 by the set of objectives 8 and - through the semi-reflective optics 4 reaches the photosensitive sensor 12.
- the photosensitive sensor 12 consists of a fast camera while the photosensitive detector 11 is a camera sensitive to the phenomena of light interaction generated at the level of the camera. sample and whose sampling rates are generally lower. Thereafter, we will call this camera the "scientific camera”.
- a combination of semi-reflective optics 4, 5 makes it possible to orient a portion of the luminous flux 13 towards the fast camera 12 and another part of the Luminous flux 15 to the scientific camera 11.
- the scientific camera 11 is not a fast camera because its sensitivity to the wavelengths emitted by the sample 9 excited by the exciter light source 3 is insufficient.
- the present invention also relates to a device for controlling the focal plane on an area of interest 10 of the sample 9 and the position of said sample, as illustrated in FIG.
- control unit programmed to control the relative position of the sample 9 with respect to the set of objectives 8 and / or the focusing.
- Said control unit may advantageously be a programmable logic array (FPGA ...) or any other device that can be programmed to perform automation (graphic card, processor, DSP, etc.). All these devices 21 are - as such - well known to those skilled in the art.
- Control is understood here as any form of servocontrol - regulation or servocontrol - and by any form of algorithm (proportional, proportional-integrator, proportional-integrator-derivator ). Regulation is defined as an enslavement with fixed and constant set point over time, the system compensating the effects of disturbance.
- the automaton compensates all the disturbing phenomena (thermal drifts, parasitic movements, etc.) which move the focal plane away from this predetermined position.
- the enslavement means a so-called follower system, for which the setpoint varies in real time according to the sensor data updated regularly.
- the data acquired by the fast camera 12 are exploited in this sense by the control unit 21 to compensate for disturbing phenomena by feedback on the motorized translation plate 17 to correct the position of the area of interest 10 under the microscope 1 and / or the game of objectives8 to correct the focus.
- this system measures and compensates for disturbing phenomena while a displacement can be imposed to follow a moving particle in the three directions of space for example.
- a programmable network to perform said servocontrol allows a hardware transcription of the logic functions. Therefore, the controller is real time and provides better reliability to said device.
- a real-time automaton is deterministic: it responds to the evolutions and demands of the environment with which it is in relation in a fixed and constant time.
- the scientific data measured by the scientific camera 11 are transmitted to a computer 20 or any terminal having a graphical interface. They can also be stored on a memory internal to the camera and transferred then on a mobile support, key type usb, dvd ...
- FIGS 4, 5 and 6 schematically describe the two main modes of operation of the present invention.
- FIG. 4 describes a simplified timing diagram which illustrates an embodiment with two cameras operating in parallel as described in FIG. 2.
- the first camera is the scientific camera 11 able to carry out fluorescence imaging on samples 9 which are naturally fluorescent or marked by fluophores.
- the excitation of the samples 9 is done through a main light source not shown whose wavelength is in the UV or the visible between 200 nm and 600 nm.
- the light fluoresced by the sample is, for its part, in a wavelength range of, for example, between 400 and 780 nm.
- the sensitivity of the scientific camera 12 is optimal for this wavelength range.
- the second camera is the fast camera 12 integrating for example a CMOS or sCMOS sensor which has an increased sensitivity. She is able to detecting a transmission image of the sample 9, illuminated by a secondary light source 2 whose wavelength does not interfere with the wavelength ranges exploited by the scientific camera 11. For example, the fast camera acquires transmission images with intense light shifted in the red to obtain sufficient contrast.
- the secondary lighting source 2 is an Electro Luminescent Diode that emits at 800 nm. It may furthermore integrate a programmable logic network 21 which makes it possible to process the images in real time and to deliver at high frequency data for the control loop of the translation stage 17 and / or the microscope 1.
- the scientific camera 11 makes video recordings at a frequency typically close to 25 frames per second.
- the integration times of the scientific camera 11 are necessarily lengthened, of the order of 30 ms for example.
- the blocks 30a, 30b, 30c of the chronogram represent these periods of recording of an image by the scientific camera 11 during the video stream.
- the interval 33a, 33b, 33c between two images is therefore of the order of 10 ms.
- the integration time of these cameras must be sufficient to obtain a level of detail and contrast on the image sufficient to calculate a similarity criterion that is used in a servo algorithm (by detection of the maximum contrast or the maximum correlation example). It is not necessary to obtain the same resolution and the same level of detail as on the scientific camera 11. This is the reason why the integration time of the fast camera 12 is lower, typically of the order of 1 ms in order to be able to record at least ten images for each image recorded by the scientific camera 11. These images are then processed by the control unit 21 to determine the parameter which will serve as a back-check on the various axes of the stage translation 17 and / or microscope 1 which are thus enslaved. This servocontrol is performed during the inter-image period 33a, 33b, 33c at during which the specific commands are sent to the actuators in order to correct the positions in the sample space 9 and / or the focus.
- FIG. 5 illustrates a timing diagram for another embodiment of the present invention with a single camera, preferably the fast camera 12 as illustrated in FIG. 1.
- This is a sequential imaging mode for which imaging fast and scientific imaging are provided by the same camera.
- the timing diagram illustrates a hybrid recording sequence in which fast recording sequences succeed one another at slower ones. Indeed, it is necessary to adapt the integration time of the camera 12 according to the destination of the recorded images. If it is scientific images30a, 30b, 30c, then it is necessary to obtain enough detail to be exploitable. In this case, the integration time of the fast camera 12 is extended, for example to 30 ms. During the interval between two scientific images, a series of images 34a, 34b, 34c is recorded continuously and at a higher frequency.
- the control unit 21 uses these images to position and / or focus the sample 9 under the microscope 1.
- it is necessary to increase the frequency of sampling, at the expense of integration time and the level of detail recorded.
- the point is not, again, to record sufficiently detailed images to be scientifically exploitable, but only the level of detail sufficient to obtain sufficient contrast and / or detail for calculate a similarity criterion and operate an algorithm based for example on the detection of the maximum contrast or correlation.
- the integration time of the images 34a, 34b, 34c is of the order of 1 ms.
- the control unit 21 can control the translation stage 17 and / or the set of objectives 8 in order to adjust the position and / or the focus of the sample 9 compared to the microscope 1 to compensate for any drifts and parasitic movements that would have been detected.
- the single-camera sequential imaging embodiment provides slightly lower scientific imaging sampling rates than in the case of the first dual-camera embodiment in parallel.
- this scientific imaging sampling frequency is of the order of 20 images per second.
- it offers the advantage of using only one fast camera, the costs are therefore reduced and the optical integration on the microscope is facilitated.
- the illumination source 3 illuminates the light. sample 9 to make it fluoresce; and the secondary lighting source 2 is off.
- the exciter source 3 is extinguished and the secondary source 2 illuminates the sample in order to increase the contrast of the image area. interest 10 on which the control unit 21 realizes the servo.
- FIG. 6 illustrates another timing diagram for a particular embodiment of the invention and which concerns the maintenance of focus during the imaging of an area of interest 10 on a sample 9.
- the objective solved by the present invention is here to keep in the focal plane a particular area of interest 10 identified at the beginning of observation throughout the duration of the observation.
- a series of fast images 34 are recorded at a higher frequency, as previously described.
- the vertical position of the sample 9 is modified around the original position in order to produce images at several altitudes and thus determine the best readjusted focal plane.
- the determination of the best focal plane among the series of fast images thus recorded is carried out during the interval 35 by maximizing a function (a similarity criterion) - for example of contrast or correlation with respect to the first image made at the beginning of 'observation.
- the new vertical position, corresponding to the best focal plane is thus calculated in real time between two scientific images 30a and 30b.
- the focus is adjusted by moving the set of objectives 8 or the translation plate 17 to the new position thus calculated.
- the reduction of integration times during the fast imaging used for servocontrol is compensated by more powerful secondary lighting. In this way, the system corrects focal plane drift errors during microscopic observation, and motion blur is minimized.
- the present invention is much faster because it relies on the new use of a fast camera. Moreover, it makes it possible to focus on the object of interest 10 which is actually imaged rather than on a reference area decorrelated from said area of interest. From a metrological point of view, the present invention is more accurate and more reliable.
- the device may be supplemented by means for micrometric manipulation of the sample in the optical field of the microscope.
- This means may be, for example, optical tongs - well known to those skilled in the art - to handle at least a portion of the sample 9 non-invasively and without contact.
- the invention also relates to the field of 3D optical microscopy object reconstruction for which a succession of images of the same object are produced at different focal planes in order to recombine them and to obtain a clear image of the object. thus imaged on all its height.
- the present invention can thus make it possible to perform such acquisitions with better accuracy and at a higher speed.
- the present invention may include certain co-location techniques for which measurements of several physical quantities are performed using an instrument on the same object. For example, in the field of optical microscopy, it may be advantageous to combine fluorescence measurements with certain markers with a "conventional" optical image in order to highlight the spatial extent of a given phenomenon.
- the present invention thus makes it possible to achieve this co-location in better conditions since it ensures that the focus and / or the position of the measured object is stable in the field of the microscope.
- the invention is not limited to the examples that have been described and many adjustments can be made to these examples without departing from the scope of the invention.
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Engineering & Computer Science (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Multimedia (AREA)
- Microscoopes, Condenser (AREA)
Abstract
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR1456964A FR3023925B1 (fr) | 2014-07-18 | 2014-07-18 | Systeme de trajectographie automatique pour microscope optique base sur une camera rapide intelligente |
PCT/EP2015/066214 WO2016008952A1 (fr) | 2014-07-18 | 2015-07-15 | Systeme de trajectographie automatique pour microscope optique base sur une camera rapide intelligente |
Publications (1)
Publication Number | Publication Date |
---|---|
EP3170045A1 true EP3170045A1 (fr) | 2017-05-24 |
Family
ID=52450230
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP15741521.7A Withdrawn EP3170045A1 (fr) | 2014-07-18 | 2015-07-15 | Systeme de trajectographie automatique pour microscope optique base sur une camera rapide intelligente |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP3170045A1 (fr) |
FR (1) | FR3023925B1 (fr) |
WO (1) | WO2016008952A1 (fr) |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7141773B2 (en) * | 2001-08-06 | 2006-11-28 | Bioview Ltd. | Image focusing in fluorescent imaging |
US7576307B2 (en) * | 2007-04-30 | 2009-08-18 | General Electric Company | Microscope with dual image sensors for rapid autofocusing |
-
2014
- 2014-07-18 FR FR1456964A patent/FR3023925B1/fr not_active Expired - Fee Related
-
2015
- 2015-07-15 EP EP15741521.7A patent/EP3170045A1/fr not_active Withdrawn
- 2015-07-15 WO PCT/EP2015/066214 patent/WO2016008952A1/fr active Application Filing
Non-Patent Citations (2)
Title |
---|
None * |
See also references of WO2016008952A1 * |
Also Published As
Publication number | Publication date |
---|---|
FR3023925B1 (fr) | 2018-01-26 |
WO2016008952A1 (fr) | 2016-01-21 |
FR3023925A1 (fr) | 2016-01-22 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP4716148B1 (ja) | 検査装置並びに欠陥分類方法及び欠陥検出方法 | |
EP2394194B1 (fr) | Procede et dispositif d'acquisition de signaux en microscopie laser a balayage | |
FR3020141A1 (fr) | Appareil et procede de microscopie a balayage de faisceau optique | |
EP3069185B1 (fr) | Dispositif et methode de mise au point tridimensionnelle pour microscope | |
EP1740974A1 (fr) | Procede et systeme de mesure de vitesse du flux sanguin | |
EP1955101A1 (fr) | Dispositif d'imagerie a balayage par fluorescence | |
EP3241014B1 (fr) | Système d'analyse d'un échantillon transparent avec contrôle de position, et procédé associé | |
FR2572515A1 (fr) | Dispositif de detection de position | |
EP3704471A1 (fr) | Appareil et procede de microscopie a fluorescence a super-resolution et de mesure de temps de vie de fluorescence | |
EP2649431B1 (fr) | Systeme et procede d'imagerie multitechniques pour l'analyse chimique, biologique ou biochiimique d'un echantillon. | |
FR3092395A1 (fr) | Dispositifs d’analyse de front d’onde et systèmes d’imagerie microscopique comprenant de tels dispositifs d’analyse | |
EP3724709B1 (fr) | Microscopie 3d spectrale en champ large | |
EP3724725B1 (fr) | Procede de calibration d'un dispositif d'analyse et dispositif associe | |
JP4844694B2 (ja) | 検査装置及び欠陥分類方法 | |
EP3170045A1 (fr) | Systeme de trajectographie automatique pour microscope optique base sur une camera rapide intelligente | |
WO2004066015A1 (fr) | Systeme de microscopie laser confocale parallele base sur la technologie vcsel | |
WO2011045530A1 (fr) | Procede et systeme d ' imagerie par fonctionnalisation du substrat | |
WO2016203163A1 (fr) | Système et procédé de collection pour spectroscopie optique | |
FR2646523A1 (fr) | Procede d'harmonisation des axes de detecteurs opto-electroniques | |
WO2017064440A1 (fr) | Dispositif optique de détection | |
FR3076911A1 (fr) | Dispositif d’eclairage de microscope permettant de mesurer un defaut de mise au point | |
WO2023052723A1 (fr) | Dispositif de détermination d'informations polarimétriques d'un faisceau lumineux et procédé associé | |
JP2003254963A (ja) | 生物分子の力学的測定装置 | |
FR3098930A1 (fr) | Dispositif d’observation d’une cellule ou d’un ensemble de cellules vivantes | |
WO2011086290A1 (fr) | Procede et systeme d'analyse moleculaire a commutation de mesures d'imagerie et de spectroscopie |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE |
|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE |
|
17P | Request for examination filed |
Effective date: 20170214 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
|
AX | Request for extension of the european patent |
Extension state: BA ME |
|
DAV | Request for validation of the european patent (deleted) | ||
DAX | Request for extension of the european patent (deleted) | ||
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: EXAMINATION IS IN PROGRESS |
|
17Q | First examination report despatched |
Effective date: 20190507 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 20190918 |