EP3087201A4 - Integrated photonic electronic sensor arrays for nucleic acid sequencing - Google Patents

Integrated photonic electronic sensor arrays for nucleic acid sequencing Download PDF

Info

Publication number
EP3087201A4
EP3087201A4 EP14873567.3A EP14873567A EP3087201A4 EP 3087201 A4 EP3087201 A4 EP 3087201A4 EP 14873567 A EP14873567 A EP 14873567A EP 3087201 A4 EP3087201 A4 EP 3087201A4
Authority
EP
European Patent Office
Prior art keywords
nucleic acid
sensor arrays
acid sequencing
electronic sensor
integrated photonic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP14873567.3A
Other languages
German (de)
French (fr)
Other versions
EP3087201A1 (en
EP3087201B1 (en
Inventor
Xing Su
Mark Oldham
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Intel Corp
Original Assignee
Intel Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Intel Corp filed Critical Intel Corp
Publication of EP3087201A1 publication Critical patent/EP3087201A1/en
Publication of EP3087201A4 publication Critical patent/EP3087201A4/en
Application granted granted Critical
Publication of EP3087201B1 publication Critical patent/EP3087201B1/en
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4145—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/543—Immobilised enzyme(s)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/157—A reaction step characterised by the number of molecules incorporated or released
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/116—Nucleic acid detection characterized by the use of physical, structural and functional properties electrical properties of nucleic acids, e.g. impedance, conductivity or resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/607—Detection means characterised by use of a special device being a sensor, e.g. electrode

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP14873567.3A 2013-12-27 2014-11-21 Integrated photonic electronic sensor arrays for nucleic acid sequencing Not-in-force EP3087201B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14/142,603 US9593371B2 (en) 2013-12-27 2013-12-27 Integrated photonic electronic sensor arrays for nucleic acid sequencing
PCT/US2014/066827 WO2015099917A1 (en) 2013-12-27 2014-11-21 Integrated photonic electronic sensor arrays for nucleic acid sequencing

Publications (3)

Publication Number Publication Date
EP3087201A1 EP3087201A1 (en) 2016-11-02
EP3087201A4 true EP3087201A4 (en) 2017-06-28
EP3087201B1 EP3087201B1 (en) 2019-07-03

Family

ID=53479496

Family Applications (1)

Application Number Title Priority Date Filing Date
EP14873567.3A Not-in-force EP3087201B1 (en) 2013-12-27 2014-11-21 Integrated photonic electronic sensor arrays for nucleic acid sequencing

Country Status (5)

Country Link
US (2) US9593371B2 (en)
EP (1) EP3087201B1 (en)
KR (1) KR101856050B1 (en)
TW (1) TWI592493B (en)
WO (1) WO2015099917A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10249741B2 (en) * 2014-05-13 2019-04-02 Joseph T. Smith System and method for ion-selective, field effect transistor on flexible substrate
JP6794350B2 (en) 2014-07-02 2020-12-02 ライフ テクノロジーズ コーポレーション Surface treatment of semiconductor sensor
US9818655B2 (en) 2015-12-08 2017-11-14 International Business Machines Corporation Method and structure for flip-chip package reliability monitoring using capacitive sensors groups
IL301735A (en) * 2016-04-22 2023-05-01 Illumina Inc Photonic stucture-based devices and compositions for use in luminescent imaging of multiple sites within a pixel, and methods of using the same
US10915023B2 (en) * 2017-11-03 2021-02-09 International Business Machines Corporation Nitrogen heterocycle-containing monolayers on metal oxides for binding biopolymers
WO2019222612A1 (en) * 2018-05-17 2019-11-21 Georgia Tech Research Corporation Devices, systems, and methods of electronic modulation of polymerase for dna synthesis

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090305909A1 (en) * 2005-06-10 2009-12-10 Life Technologies Corporation Method and System for Multiplex Genetic Analysis
US20110319276A1 (en) * 2010-06-25 2011-12-29 Jianquan Liu Nucleotides and oligonucleotides for nucleic acid sequencing
US20120046176A1 (en) * 2010-08-20 2012-02-23 Xing Su Nucleic acid sequencing
US20120220471A1 (en) * 2008-06-11 2012-08-30 Stichting Imec Nederland Nanoantenna and uses thereof for biosensing
US20130109577A1 (en) * 2011-10-14 2013-05-02 Pacific Biosciences Of California, Inc. Real-time redox sequencing
WO2015074004A1 (en) * 2013-11-17 2015-05-21 Quantum-Si Incorporated Integrated device with external light source for probing detecting and analyzing molecules

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5565322A (en) * 1991-11-07 1996-10-15 Nanogen, Inc. Hybridization of polynucleotides conjugated with chromophores and fluorophores to generate donor-to donor energy transfer system
US6342389B1 (en) * 1995-04-10 2002-01-29 Roger S. Cubicciotti Modified phycobilisomes and uses therefore
US6060023A (en) * 1998-03-31 2000-05-09 Motorola, Inc. Molecular sensing apparatus
AU4833899A (en) * 1998-06-24 2000-01-10 Therasense, Inc. Multi-sensor array for electrochemical recognition of nucleotide sequences and methods
US6288221B1 (en) * 1998-08-20 2001-09-11 Duke University Methods of synthesis of halogen base-modified oligonucleotides and subsequent labeling with a metal-catalyzed reaction
US20030143556A1 (en) * 2001-04-03 2003-07-31 Gary Blackburn Nucleic acid reactions using labels with different redox potentials
AU2002365115A1 (en) * 2001-07-20 2003-09-02 North Carolina State University Light addressable electrochemical detection of duplex structures
JP2004206412A (en) * 2002-12-25 2004-07-22 Casio Comput Co Ltd Card type device and authentication system
GB0317343D0 (en) * 2003-07-24 2003-08-27 Medical Biosystems Ltd Polynucleotide sequencing
US7592539B2 (en) * 2003-11-07 2009-09-22 The Trustees Of Princeton University Solid state photosensitive devices which employ isolated photosynthetic complexes
JP3903183B2 (en) * 2004-02-03 2007-04-11 独立行政法人物質・材料研究機構 Gene detection field effect device and gene polymorphism analysis method using the same
US7615787B2 (en) * 2004-03-26 2009-11-10 Canon Kabushiki Kaisha Photo-semiconductor device and method of manufacturing the same
WO2006022370A1 (en) 2004-08-27 2006-03-02 National Institute For Materials Science Method of analyzing dna sequence using field-effect device, and base sequence analyzer
US9695472B2 (en) 2005-03-04 2017-07-04 Intel Corporation Sensor arrays and nucleic acid sequencing applications
US9040237B2 (en) 2005-03-04 2015-05-26 Intel Corporation Sensor arrays and nucleic acid sequencing applications
JP5320077B2 (en) * 2006-03-03 2013-10-23 ユニベルシテ パリ ディドロ−パリ ７ Method for electrochemical detection of target nucleic acid sequence
US7923240B2 (en) 2006-03-31 2011-04-12 Intel Corporation Photo-activated field effect transistor for bioanalyte detection
AU2008276308A1 (en) 2007-07-13 2009-01-22 The Board Of Trustees Of The Leland Stanford Junior University Method and apparatus using electric field for improved biological assays
EP2212679A1 (en) * 2007-09-18 2010-08-04 Applied Biosystems Inc. Methods, systems and apparatus for light concentrating mechanisms
US7960116B2 (en) 2007-09-28 2011-06-14 Pacific Biosciences Of California, Inc. Nucleic acid sequencing methods and systems
WO2009082706A1 (en) 2007-12-21 2009-07-02 The Trustees Of Columbia University In The City Of New York Active cmos sensor array for electrochemical biomolecular detection
US8372585B2 (en) 2007-12-31 2013-02-12 Intel Corporation Electronic sensing for nucleic acid sequencing
US8084202B2 (en) 2008-03-31 2011-12-27 Intel Corporation Optical detection for electronic microarrays
US20110250402A1 (en) * 2008-06-02 2011-10-13 Applied Biosystems, Llc Localization of near-field resonances in bowtie antennae: influence of adhesion layers
WO2009149125A2 (en) 2008-06-02 2009-12-10 Life Technologies Corporation Localization of near-field resonances in bowtie antennae: influence of adhesion layers
US8500979B2 (en) * 2009-12-31 2013-08-06 Intel Corporation Nanogap chemical and biochemical sensors
US8865077B2 (en) * 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US9075010B2 (en) * 2010-10-15 2015-07-07 The Board Of Trustees Of The Leland Stanford Junior Univerity Enhancement of molecular emission using optical-antenna structures
WO2012086772A1 (en) * 2010-12-24 2012-06-28 Ｎｅｃソフト株式会社 Analytical device and analytical method
KR101729489B1 (en) * 2011-03-11 2017-04-24 (주) 하임바이오텍 Biosensor utilizing conductive particles and the combination of polynucleotides corresponding to conductive particles, and method for detecting electric signal using the same
WO2012162429A2 (en) * 2011-05-23 2012-11-29 The Trustees Of Columbia University In The City Of New York Dna sequencing by synthesis using raman and infrared spectroscopy detection
CA2858036C (en) 2011-12-15 2017-08-22 Intel Corporation Diamond electrode nanogap transducers
WO2013100949A1 (en) 2011-12-28 2013-07-04 Intel Corporation Nanogap transducers with selective surface immobilization sites
US8969781B2 (en) * 2012-06-28 2015-03-03 Board Of Regents, The University Of Texas System Integrated optical biosensor array including charge injection circuit and quantizer circuit
US9551682B2 (en) 2012-06-29 2017-01-24 Intel Corporation High throughput biochemical detection using single molecule fingerprinting arrays
US8741117B2 (en) 2012-09-27 2014-06-03 Intel Corporation Device and method for detecting redox reactions in solution
US9222908B2 (en) 2012-09-27 2015-12-29 Intel Corporation Device and method for detecting redox reactions in solution
US20140178862A1 (en) * 2012-12-20 2014-06-26 Xing Su Photoinduced redox current (pirc) detection for dna sequencing using integrated transducer array
WO2014182630A1 (en) * 2013-05-06 2014-11-13 Pacific Biosciences Of California , Inc. Real-time electronic sequencing

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090305909A1 (en) * 2005-06-10 2009-12-10 Life Technologies Corporation Method and System for Multiplex Genetic Analysis
US20120220471A1 (en) * 2008-06-11 2012-08-30 Stichting Imec Nederland Nanoantenna and uses thereof for biosensing
US20110319276A1 (en) * 2010-06-25 2011-12-29 Jianquan Liu Nucleotides and oligonucleotides for nucleic acid sequencing
US20120046176A1 (en) * 2010-08-20 2012-02-23 Xing Su Nucleic acid sequencing
US20130109577A1 (en) * 2011-10-14 2013-05-02 Pacific Biosciences Of California, Inc. Real-time redox sequencing
WO2015074004A1 (en) * 2013-11-17 2015-05-21 Quantum-Si Incorporated Integrated device with external light source for probing detecting and analyzing molecules

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2015099917A1 *

Also Published As

Publication number Publication date
KR101856050B1 (en) 2018-06-20
EP3087201A1 (en) 2016-11-02
TWI592493B (en) 2017-07-21
WO2015099917A1 (en) 2015-07-02
EP3087201B1 (en) 2019-07-03
US20150184237A1 (en) 2015-07-02
US20170183727A1 (en) 2017-06-29
US9593371B2 (en) 2017-03-14
KR20160075745A (en) 2016-06-29
TW201538731A (en) 2015-10-16
US10513734B2 (en) 2019-12-24

Similar Documents

Publication Publication Date Title
EP3019083A4 (en) Sensor array system
EP3085764A4 (en) Nucleic acid extraction apparatus
IL241426A0 (en) Nucleic acid amplification
IL241269A0 (en) Nucleic acid amplification
EP2807477A4 (en) Analyte sensor
LT4056105T (en) Analyte sensor devices
EP2844333A4 (en) Sensor array
EP3077545A4 (en) Methods for sequencing nucleic acids
EP3028265A4 (en) Displacement sensor
EP3055431A4 (en) Synthetic long read dna sequencing
EP2971159A4 (en) Methods for analyzing nucleic acids
GB201419367D0 (en) Nucleic acid probe
EP3090241A4 (en) Magnetoelastic sensor
EP2992297A4 (en) Bipartite sensor array
EP3087201A4 (en) Integrated photonic electronic sensor arrays for nucleic acid sequencing
EP3055413A4 (en) Detection of chemical modifications in nucleic acids
EP2891709A4 (en) Nucleic acid analysis device
EP3066218A4 (en) Methods for detecting nucleic acids
EP3084051A4 (en) Fine-tuned ultraspecific nucleic acid hybridization probes
EP3044598A4 (en) Connector with sensor
EP3081898A4 (en) Displacement sensor
HK1232259A1 (en) Nucleic acid amplification
IL241273A0 (en) Nucleic acid amplification
EP3069377A4 (en) Silicon nanowire-based sensor arrays
EP2966404A4 (en) Physical quantity sensor structure

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20160602

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20170526

RIC1 Information provided on ipc code assigned before grant

Ipc: C12M 1/42 20060101ALI20170519BHEP

Ipc: G01N 21/77 20060101ALI20170519BHEP

Ipc: C12Q 1/68 20060101AFI20170519BHEP

Ipc: G01N 21/64 20060101ALI20170519BHEP

Ipc: G01N 27/26 20060101ALI20170519BHEP

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: EXAMINATION IS IN PROGRESS

17Q First examination report despatched

Effective date: 20180202

REG Reference to a national code

Ref country code: DE

Ref legal event code: R079

Ref document number: 602014049663

Country of ref document: DE

Free format text: PREVIOUS MAIN CLASS: C12Q0001680000

Ipc: C12Q0001687400

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: GRANT OF PATENT IS INTENDED

RIC1 Information provided on ipc code assigned before grant

Ipc: G01N 21/77 20060101ALI20190108BHEP

Ipc: C12M 1/42 20060101ALI20190108BHEP

Ipc: C12Q 1/6874 20180101AFI20190108BHEP

INTG Intention to grant announced

Effective date: 20190125

RIN1 Information on inventor provided before grant (corrected)

Inventor name: SU, XING

Inventor name: OLDHAM, MARK

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE PATENT HAS BEEN GRANTED

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

Ref country code: AT

Ref legal event code: REF

Ref document number: 1151051

Country of ref document: AT

Kind code of ref document: T

Effective date: 20190715

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 602014049663

Country of ref document: DE

REG Reference to a national code

Ref country code: NL

Ref legal event code: FP

REG Reference to a national code

Ref country code: LT

Ref legal event code: MG4D

REG Reference to a national code

Ref country code: AT

Ref legal event code: MK05

Ref document number: 1151051

Country of ref document: AT

Kind code of ref document: T

Effective date: 20190703

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

Ref country code: LT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

Ref country code: BG

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20191003

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20191003

Ref country code: AT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20191104

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: NL

Payment date: 20191114

Year of fee payment: 6

Ref country code: DE

Payment date: 20191105

Year of fee payment: 6

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: AL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

Ref country code: ES

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

Ref country code: RS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20191004

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20191103

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FR

Payment date: 20191029

Year of fee payment: 6

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: TR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

Ref country code: DK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20191122

Year of fee payment: 6

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SM

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20200224

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 602014049663

Country of ref document: DE

REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

PG2D Information on lapse in contracting state deleted

Ref country code: IS

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CH

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20191130

Ref country code: LU

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20191121

Ref country code: MC

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

Ref country code: LI

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20191130

26N No opposition filed

Effective date: 20200603

REG Reference to a national code

Ref country code: BE

Ref legal event code: MM

Effective date: 20191130

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20191121

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: BE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20191130

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CY

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

REG Reference to a national code

Ref country code: DE

Ref legal event code: R119

Ref document number: 602014049663

Country of ref document: DE

REG Reference to a national code

Ref country code: NL

Ref legal event code: MM

Effective date: 20201201

GBPC Gb: european patent ceased through non-payment of renewal fee

Effective date: 20201121

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703

Ref country code: HU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT; INVALID AB INITIO

Effective date: 20141121

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: NL

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20201201

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FR

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20201130

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GB

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20201121

Ref country code: DE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20210601

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190703