EP2847340A2 - Mehrstufiges fermentationsverfahren ausgehend von synthesegas - Google Patents

Mehrstufiges fermentationsverfahren ausgehend von synthesegas

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Publication number
EP2847340A2
EP2847340A2 EP13728123.4A EP13728123A EP2847340A2 EP 2847340 A2 EP2847340 A2 EP 2847340A2 EP 13728123 A EP13728123 A EP 13728123A EP 2847340 A2 EP2847340 A2 EP 2847340A2
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EP
European Patent Office
Prior art keywords
acetate
microorganism
clostridium
ethanol
process step
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EP13728123.4A
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German (de)
English (en)
French (fr)
Inventor
Thomas Haas
Eva Maria Wittmann
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Evonik Operations GmbH
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Evonik Industries AG
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Publication of EP2847340A2 publication Critical patent/EP2847340A2/de
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
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    • C12P7/00—Preparation of oxygen-containing organic compounds
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/54—Acetic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Definitions

  • the invention relates to processes for the preparation of hydrocarbons substituted by at least one group containing at least one oxygen atom, comprising the process steps
  • C0 2 as a carbon source for the synthesis of organic compounds in microbiological processes is often described in the literature.
  • the object of the invention was to provide a method which is able to overcome at least one disadvantage of the prior art.
  • the present invention therefore provides a method as described in claim 1 and the other independent claims.
  • An advantage of the present invention is that C0 2 / CO mixtures are a much cheaper raw material, which can also be produced from various sources, such as natural gas and biogas, coal, oil and plant residues.
  • Another advantage of the method according to the invention is the high carbon yield.
  • the C0 2 can namely be converted back into acetic acid in the first stage.
  • Fermentation conditions, as for the actual production in the process step C) according to the invention another organism is used, as in the carbon fixation in the acetate.
  • Yet another advantage of the present invention is that by the use of acetate and / or ethanol, in particular acetate, as the C source in process step C) are different Product compositions may result as if a sugar is used in process step C).
  • An object of the present invention is a process for the preparation of at least one group containing at least one oxygen atom
  • Hydrocarbons comprising the process steps
  • At least one hydrocarbon-substituted group containing at least one oxygen atom and optionally with a second microorganism
  • acetate in the context of the present invention is to be understood as meaning both acetic acid and its salts, which necessarily results from the fact that the
  • Microorganisms work in an aqueous environment, and thus always there is a balance between salt and acid.
  • second microorganism in connection with the present invention is to be understood as other than the "first microorganism” from process step A). All percentages (%) are by mass unless otherwise specified.
  • the source of the substrates carbon dioxide and / or carbon monoxide
  • any gas or mixture of gases capable of containing the microorganisms can be used to supply sufficient amounts of carbon so that they can form acetate and / or ethanol.
  • the carbon source be provided by exhaust gases such as synthesis gas, flue gas, petroleum refinery emissions, yeast fermentation or clostridial fermentation gases, gasification gases from cellulosic materials, or coal gasification.
  • exhaust gases such as synthesis gas, flue gas, petroleum refinery emissions, yeast fermentation or clostridial fermentation gases, gasification gases from cellulosic materials, or coal gasification.
  • At least part of the carbon dioxide and / or carbon monoxide is a by-product of process step C) of the process according to the invention. This has the technical effect that the
  • Synthesis gas can e.g. be provided from the by-product of coal gasification.
  • the microorganism thus converts a substance, which is a waste product, into a valuable raw material.
  • synthesis gas may be provided by the gasification of widely available, inexpensive agricultural raw materials for the process of the present invention.
  • Raw materials are selected from the group comprising perennial grasses such as miscanthus,
  • Grain residues processing waste such as sawdust.
  • synthesis gas is obtained in a gasification apparatus from dried biomass, mainly by pyrolysis, partial oxidation and steam reforming, wherein the
  • the carbon source in process step A) contains at least 50% by weight, preferably at least 70% by weight, particularly preferably at least 90% by weight of CO 2 and / or CO, where the wt % to all carbon sources available to the microorganism in step A).
  • process step A preference is given to adding a reducing agent, preferably hydrogen, together with the carbon dioxide and / or carbon monoxide to the reaction.
  • a reducing agent preferably hydrogen
  • a method preferred according to the invention is characterized in that the carbon source in method step A) comprises synthesis gas, in particular consists of synthesis gas.
  • Microorganisms which convert C0 2 and / or CO to acetate and / or ethanol, in particular acetate, and suitable processes and process conditions which are used in process step A) have been known for a long time. Such processes are described, for example, in WO9800558, WO2000014052, WO2010115054
  • thermoaceticum Effect of metals on growth yield, enzymes, and the synthesis of acetate from carbon dioxide. Journal of Bacteriology (1973), 1 14 (2), 743-51.
  • acetogenic bacteria particularly suitable in this context are acetogenic bacteria.
  • the group of acetogenic bacteria belongs to the anaerobic prokaryotes, the C0 2 as terminal
  • an acetogenic bacterium is used as the first microorganism.
  • acetogenic bacteria selected from the group comprising Clostridium autothenogenum DSMZ 19630, Clostridium ragsdahlei ATCC no. BAA-622, Clostridium autoethanogenum, Moorella sp HUC22-1, Moorella thermoaceticum, Moorella
  • thermoautotrophica Rumicoccus productus, Acetoanaerobum, Oxobacter pfennigii,
  • Methanosarcina barkeri Methanosarcina acetivorans, Carboxydothermus, Desulfotomaculum kutznetsovii, Pyrococcus, Peptostreptococcus, Butyribacterium methylotrophicum ATCC 33266, Clostridium formicoaceticum, butyricum Clostridium, Lactobacillus delbrukii, Propionibacterium acidoprprionici, Proprionispera arboris, Anaerobierspirillum succiniproducens, Bacteroides amylophilus, Becterioides ruminicola, kivui Thermoanaerobacter, Acetobacterium woodii, Acetoanaerobium notera, Clostridium aceticum, Butyribacterium methylotrophicum, Moorella thermoacetica, Eubacterium limosum, Peptostreptococcus productus, Clostridium ljungdahli
  • Clostridium ljungdahlii is Clostridium ljungdahlii, in particular strains selected from the group comprising Clostridium ljungdahlii PETC, Clostridium ljungdahlii ERI2, Clostridium ljungdahlii C0I and Clostridium ljungdahlii 0-52, these are described in WO 98/00558 and WO 00/68407, and ATCC 49587, ATCC 55988 and ATCC 55989.
  • ethanol is formed in process step A) and, as microorganism, alkali baculum bacchi ATCC BAA-1772, Moorella sp. HUC22-1, Clostridium ljungdahlii, Clostridium ragsdahlei, or Clostridium autoethanogenum.
  • Corresponding instructions for carrying out process step A) are obtained, for example, from Saxena et al. Clostridium ragsdalei. By the ethanologenic acetogen. Journal of Industrial Microbiology & Biotechnology Volume 38, Number 4 (201 1), 513-521, Younesi et al. Ethanol and acetate production from synthesis gas via fermentation processes using anaerobic bacterium clostridium ljungdahlii. Biochemical Engineering Journal Volume 27, Issue 2, 15 December 2005, Pages 110-119,
  • Process step A) is preferably carried out under anaerobic conditions.
  • process step B) of the process according to the invention the acetate formed in process step A) and / or ethanol, in particular acetate, is separated from the first microorganism.
  • the microorganisms are removed, for example, by known methods such as sedimentation, centrifugation or filtration as a solid from the medium containing the acetate and / or ethanol, in particular acetate, and optionally the remaining liquid phase is fed directly to process step C).
  • the direct feeding has the advantage that optionally from process step A) additionally contained
  • the acetate itself can be removed from the microorganisms in process step A) by means of extraction, in particular by means of in situ extraction.
  • Extraction methods are known to those skilled in the art, for example from EP2294206, WO2000014052, US 4405717, from Katikaneni et al. Purification of Fermentation-Derived Acetic Acid By Liquid-Liquid Extraction and Esterification. Ind. Eng. Chem. Res. 2002, 41, 2745-2752, and from Huh et al. Selective extraction of acetic acid from the fermentation broth produced by Mannheimia succiniciproducens. Biotechnol Lett. 2004 Oct; 26 (20): 1581 -4.
  • Suitable extractants are described, for example, under point A.
  • alkylamines or low-boiling solvents such as MTBE or ethyl acetate are preferably contained as extractants, the alkylamines preferably being those having at least 16 C atoms, preferably trialkylamines and more preferably trialkylamines selected from the group comprising trihexylamine, trioctylamine, tridecylamine, tricaprylamine, tridodecylamine.
  • These trialkylamines comprising extractants are preferably used in conjunction with an in situ extraction in step B). This has the technical effect that the first microorganism is not damaged and the additional advantage that process step B) can be carried out in a countercurrent process, which is additionally preferred.
  • the extractant used in process step B) is a mixture of trioctylamine and 2-ethyl-1-hexanol, these being preferably used in equal amounts.
  • process step C the acetate and / or ethanol, in particular acetate, is reacted with a second microorganism to form a hydrocarbon substituted with at least one group containing at least one oxygen atom.
  • Substituted hydrocarbon is preferably carboxylic acids, dicarboxylic acids, hydroxycarboxylic acids, carboxylic acid esters, hydroxycarboxylic acid esters, alcohols, aldehydes, Ketones, which in particular 4 to 32, preferably 6 to 20, particularly preferably 8 to 12 carbon atoms. Particular preference is given to carboxylic acids, hydroxycarboxylic acids and carboxylic esters.
  • the second microorganism is preferably yeast or bacteria
  • the second microorganism is preferably a genetically modified strain which has been genetically optimized in particular with respect to the yield of the hydrocarbon substituted with at least one group containing at least one oxygen atom.
  • WO2011 127409, WO200911 1672 and WO2010062480 suitable second microorganisms and process for the preparation of fatty alcohols
  • WO2010126891 for the production of alcohols, fatty acids and fatty acid esters, WO20101 18410, WO201002171 1 and WO2010022090 for the preparation of
  • the hydrocarbon substituted by at least one group containing at least one oxygen atom is fatty acids, in particular linear, saturated, fatty acids having 4 to 32, preferably 6 to 20, particularly preferably 8 to 12 carbon atoms.
  • the second microorganism is a microorganism which has an increased activity of at least one thioesterase compared to its wild type.
  • thioesterases in this connection are selected from acyl-ACP thioesterases, preferably EC 3.1.2.14 or EC 3.1.2.22 and acyl-CoA thioesterases, preferably EC 3.1.2.2, EC 3.1.2.18, EC 3.1.2.19, EC 3.1.2.20 or EC 3.1.2.22.
  • Preferred second microorganisms which are used in the alternative according to the invention are disclosed in WO20101 18410, WO2010075483, WO2008119082 and WO2007136762, the disclosure of which is expressly referred to with regard to these microorganisms and with respect to these thioesterases.
  • the fatty acid is octane and / or decanoic acid and thioesterase is the gene product of fatB2 from Cuphea hookeriana.
  • the hydrocarbon which is substituted by at least one group containing at least one oxygen atom is hydroxycarboxylic acids, in particular omega-hydroxycarboxylic acids or hydroxyisobutyric acids, in particular 3-hydroxyisobutyric acid.
  • Microorganism in particular to microorganisms, which in WO2009156214,
  • the disclosure content of these documents is expressly referred to with respect to this.
  • the second microorganism is in particular microorganisms which are disclosed in WO2011008232, the disclosure content of this document is expressly referred to with respect to this. It is inventively preferred that carbon dioxide formed in process step C) is fed back to the process in process step A) and thus is available as C source. This has the technical effect that the carbon yield is 100%.
  • Embodiments should be limited.
  • Figure 1 Fatty Acid Production in E. coli of Synthesis Gas Microbially Produced Acetate
  • Example 1 Process step A) Acetate and ethanol formation
  • a live culture of Clostridium carboxidivorans DSMZ 15243 was prepared in 1 l
  • Vitamin solution filled in 1 l of water.
  • the pH was adjusted to a pH of 5.9 with 0.5 M NaOH.
  • the mineral salt solution consists of 80 g of sodium chloride, 100 g of ammonium chloride, 10 g of potassium chloride, 10 g of potassium monophosphate, 20 g of magnesium sulfate, 4 g of calcium chloride per liter.
  • the vitamin solution consists of 0.01 g of pyridoxine, 0.005 g of thiamine, 0.005 g of riboflavin, 0.005 g of calcium pantothenate, 0.005 g of thioctic acid, 0.005 g of (para) -aminobenzoic acid, 0.005 g of nicotinic acid, 0.005 g of vitamin B12, 0.002 g of biotin, 0.002 g of folic acid , 0.01 g MESNA per liter.
  • the trace element solution consists of 2 g of nitriloacetic acid, 1 g of manganese sulfate, 0.8 g of iron ammonium sulfate, 0.2 g of cobalt chloride, 0.2 g of zinc sulfate, 0.02 g of copper (II) chloride, 0.02 g of nickel chloride, 0 , 02 g of sodium molybdate, 0.02 g of sodium selenate, 0.02 g of sodium tungstate per liter.
  • the medium was boiled for 20 minutes and then fumigated with pure nitrogen for 20 minutes. Thereafter, it was autoclaved for 20 minutes at 121 ° C. After cooling, the medium was fed 3 times with a gas mixture of 50% CO, 45% H 2 and 5% CO 2 up to 1 bar overpressure. Thereafter, the pressure was set to 0.8 bar overpressure. Immediately before inoculation was carried out under sterile, anaerobic conditions, the addition of 1, 5 ml of a 4% solution of sodium sulfite / cysteine hydrochloride as a reducing agent.
  • the culture was cultured at 37 ° C with 100 rpm (5 cm eccentricity). The culture was inoculated after 72 hours in a new medium.
  • the pH was adjusted to 5.9 with 0.5 M NaOH and 0.5 M HCl throughout the fermentation.
  • the gas mixture was adjusted to 80% CO and 20% CO 2 using a gas mixing station WMR 4000 from Westphal Mess- und Regeltechnik. Fumigation was constant at 5 l / h.
  • the stirrer speed was set to a constant 400 rpm, which corresponds to a power input of 0.2 W / l.
  • the inoculum was 10% and was also added under sterile, anaerobic conditions 30 minutes after the start of fumigation.
  • the starting OD 6 oo was 0.055.
  • High performance liquid chromatography determined.
  • the column Aminex HPX-87H was used as a stationary phase.
  • the eluent used was 5 mM sulfuric acid at a constant flow rate of 0.6 ml / min.
  • the temperature of the column was 40 ° C.
  • the detection of ethanol and butanol was carried out by means of a refractive index detector, for the detection of acetic acid and butyric acid, a diode array detector at a wavelength of 210 nm was used.
  • the substance concentrations were determined by the peak area on the basis of
  • the pH of the fermentation broth was lowered to a pH of less than 3.0 after separation of the cells by the addition of acetic acid. Thereafter, a tri-n-octylamine solution in 1-octanol in the ratio 1: 1 was added to the fermentation broth and mixed at a stirrer speed of at least 1000 rpm at 25 ° C for up to 2 hours with the fermentation broth. The subsequent phase separation was carried out by centrifugation. From the organic phase was then at 120 ° C and 500 mbar overpressure the acetic acid is distilled. The acetic acid content of the distillate was determined by HPLC and the solution was used in the corresponding concentration in the further fermentation (compare Example 3 and 4 and 6).
  • Example 3 Reaction of acetate to C8 and C10 fatty acids in recombinant E. coli
  • Yeast extract 10 g peptone, 0.5 g sodium chloride and 15 g agar-agar pH 7 streaked.
  • the strain E. coli JW5020-1 (AfadE) pJ294 [Ptac-ChFATB2_optEc] was streaked with an inoculation loop from a cryoculture on an LB plate containing additionally 100 mg / ml ampicillin. The plates were incubated overnight at 37 ° C.
  • the strain E. coli JW5020-1 (AfadE) pJ294 [Ptac-ChFATB2_optEc] is transformed with an expression vector for the gene fatB2 from Cuphea hookeriana.
  • this gene was codon-optimized for expression in Escherichia coli.
  • the gene was synthesized together with a fac promoter and simultaneously a
  • the synthesized DNA fragment P tac -ChFatB2 (SEQ ID NO. 1). was digested with the restriction endonucleases BamH ⁇ and Noü and ligated into the appropriately cut vector pJ294 (DNA2.0 Inc., Menlo Park, CA, USA).
  • the completed E. coli expression vector was designated pJ294 [Ptac-ChFATB2_optEc] (SEQ ID NO: 2).
  • Preculture 1 Both cultures were inoculated into 10 ml of M9, mod-G liquid medium in 100 ml shake flasks with baffles.
  • the M9 mod-G medium is composed of 2.6 g / l (NH 4 ) 2 S0 4 , 0.49 g / l MgS0 + 7H 2 0, 20 g / l glucose, 1 ml / l of trace elements US3 dissolved in 800 ml M9 buffer and 150 ml ddH 2 0.
  • the M9 buffer consists of 6.79 g / l Na 2 HPO 2 + 2H 2 0, 3 g / l KH 2 P0, 0.5 g / l NaCl, 2 g / l NH CI dissolved in 800 ml ddH 2 0.
  • 100 ⁇ g / ml ampicillin were added to the medium.
  • the cultures were incubated at 37 ° C and 200rpm overnight.
  • M9, mod-G medium is composed of 2.6 g / l (NH 4 ) 2 S0 4 , 0.49 g / l MgS0 + 7H 2 O, 60 mM sodium acetate (from Example 2), 1 ml / l Trace elements US3 dissolved in 800 ml M9 buffer and 170 ml ddH 2 0.
  • 100 ug / ml ampicillin was added for the culture of the plasmid-carrying strain.
  • the cultures were incubated at 37 ° C and 200 rpm overnight.
  • IPTG was induced with 1 mM IPTG from a stock solution of 1 M.
  • the quantification of fatty acids was carried out after derivatization as fatty acid methyl ester by gas chromatography.
  • samples consisting of 2 ml of culture broth, 50 ⁇ l of heptadecanoic acid (10 g / l dissolved in ethanol) were added as internal reference substance after addition of 1 ml of acetone and 2 ml of water.
  • the samples were acidified with 200 ⁇ acetic acid and added with 10 ml of a 1: 1 (v / v) chloroform / methanol mixture.
  • the samples were vigorously mixed for at least 1 min. Subsequently, the chloroform phase was removed and evaporated.
  • the dry residue was taken up in 1 ml of 1, 25 M methanolic hydrochloric acid and incubated at 50 ° C overnight to esterify the fatty acids.
  • the reaction was stopped by adding 5 ml of saturated Natnumcarbonatants (all substances Sigma-Aldrich, Steinheim).
  • the extraction of the fatty acid methyl esters was carried out by adding 1 ml of n-heptane and vigorous mixing for 15 seconds.
  • the heptane phase is measured by gas chromatography.
  • the capillary column SP TM -2560 with the dimensions 100 mx 0.25 mm and a film thickness of 0.2 ⁇ m (Supelco, Sigma-Aldrich, Steinheim) was used as the stationary phase.
  • Helium was used as the carrier gas. The separation took place within 45 min with an injector temperature of 260 ° C, detector temperature of 260 ° C and column temperature of 140 ° C at the beginning, held for 5 min and raised to 240 ° C at a rate of 4 ° C / min and held for 15 min. The injection volume is 1 ⁇ , the split rate 1:20 and the flow rate of the carrier gas 1 ml / min. The detection was carried out by means of flame ionization detector (GC Perkin Elmer Clarus 500, Perkin Elmer, Rodgau). Heptadecanoic acid (Sigma-Aldrich, Steinheim) was used as internal
  • Palmitic acid methyl ester C16: 1 -Me palmitoleic acid methyl ester, C18: 0-Me
  • Methyl stearate, C18: 1 Me methyl ester (GLC Standard Mix GLC-20 1892-1AMP, GLC-30 1893-1AMP, GLC-50 1894-1AMP, Sigma-Aldrich, Steinheim) were used for calibration. The limits of determination are for all fatty acid methyl esters at a concentration of 10 mg / l.
  • ChFATB2_optEc] after 96 hours normalizes to an OD of 1 as shown in Figure 1.
  • Example 4 Reaction of acetate to 3-hydroxyisobutyric acid (3-HIB) with Yarrowia lipolytica H222-41 A3HIBDH (ura) -8
  • Example 1 a Y. lipolytica cell H222-41 having a weakened activity of 3-hydroxyiosbutyric acid dehydrogenase was prepared; this cell is called H222-41 ⁇ 3HIBDH (ura) -8 below.
  • H222-41 ⁇ 3HIBDH (ura) -8 was cultured in comparison with the corresponding wild-type H222-41 (ura) -8. cultivation
  • YEPD agar plates consist of 10 g glucose, 10 g yeast extract, 20 g peptone and 15 g agar agar. The pH is 5.4. The incubation was carried out at 25 ° C for 80 hours. Preculture (biomass production)
  • Acetate medium made up to 50 ml each.
  • the van Uden acetate medium is composed as follows:
  • the OD increases in the test period from an average of 10 to an average of 45.
  • the acetate content at the beginning was on average 2250 mg / kg, at the end of the fermentation 32 mg / l.
  • the 3HIB content at the beginning of the fermentation is in both Y. lipolytica H222-41 ⁇ 3HIBDH (ura) -8 and Y. lipolytica H222-41 (ura) -8 0 mg / l.
  • Dehydrogenase has produced an average of 18 mg / kg 3HIB.
  • Example 5 Process step B) Ethanol separation The separation of ethanol in the form of an aqueous concentrate was carried out by direct distillation of the fermentation broth from Example 1.
  • Example 6 Reaction of acetate and ethanol to form hexanoic acid and ethylhexylate with the anaerobic bacterium Clostridium kluyveri
  • pressure-resistant glass bottles which can be hermetically sealed with a butyl rubber stopper, were used. All cultivation steps were carried out under anaerobic conditions. The bottles were autoclaved at 121 ° C for 20 minutes to ensure sterility.
  • Hexanoic acid / hexanoic acid ethyl ester here the total value rose from 0.05 g / L to 7.6 g / L, be shown.

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