EP2737062A4 - Near-infrared light-activated proteins - Google Patents

Near-infrared light-activated proteins

Info

Publication number
EP2737062A4
EP2737062A4 EP12817578.3A EP12817578A EP2737062A4 EP 2737062 A4 EP2737062 A4 EP 2737062A4 EP 12817578 A EP12817578 A EP 12817578A EP 2737062 A4 EP2737062 A4 EP 2737062A4
Authority
EP
European Patent Office
Prior art keywords
infrared light
activated proteins
proteins
activated
infrared
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP12817578.3A
Other languages
German (de)
French (fr)
Other versions
EP2737062A2 (en
EP2737062B1 (en
Inventor
Mark Gomelsky
Min-Hyung Ryu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Wyoming
Original Assignee
University of Wyoming
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Wyoming filed Critical University of Wyoming
Publication of EP2737062A2 publication Critical patent/EP2737062A2/en
Publication of EP2737062A4 publication Critical patent/EP2737062A4/en
Application granted granted Critical
Publication of EP2737062B1 publication Critical patent/EP2737062B1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • C12N9/6475—Interleukin 1-beta convertase-like enzymes (3.4.22.10; 3.4.22.36; 3.4.22.63)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/22056—Caspase-3 (3.4.22.56)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y406/00—Phosphorus-oxygen lyases (4.6)
    • C12Y406/01—Phosphorus-oxygen lyases (4.6.1)
    • C12Y406/01001—Aodenylate cyclase (4.6.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y406/00—Phosphorus-oxygen lyases (4.6)
    • C12Y406/01—Phosphorus-oxygen lyases (4.6.1)
    • C12Y406/01002—Guanylate cyclase (4.6.1.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
EP12817578.3A 2011-07-27 2012-07-27 Near-infrared light-activated proteins Active EP2737062B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161512065P 2011-07-27 2011-07-27
PCT/US2012/048699 WO2013016693A2 (en) 2011-07-27 2012-07-27 Near-infrared light-activated proteins

Publications (3)

Publication Number Publication Date
EP2737062A2 EP2737062A2 (en) 2014-06-04
EP2737062A4 true EP2737062A4 (en) 2015-06-10
EP2737062B1 EP2737062B1 (en) 2017-08-23

Family

ID=47597721

Family Applications (1)

Application Number Title Priority Date Filing Date
EP12817578.3A Active EP2737062B1 (en) 2011-07-27 2012-07-27 Near-infrared light-activated proteins

Country Status (4)

Country Link
US (1) US8835399B2 (en)
EP (1) EP2737062B1 (en)
DK (1) DK2737062T3 (en)
WO (1) WO2013016693A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10041057B2 (en) 2011-07-27 2018-08-07 University Of Wyoming Near-infrared light-activated proteins
US20150327924A1 (en) 2014-05-16 2015-11-19 Medtronic Ardian Luxembourg S.A.R.L. Systems, devices, and methods for modulating renal nerve tissue
US9820690B1 (en) 2014-07-16 2017-11-21 Verily Life Sciences Llc Analyte detection system
CN116875518A (en) 2018-07-06 2023-10-13 科罗拉多州立大学董事会法人团体 Gene coding system for constructing and detecting bioactive agents
WO2022245907A1 (en) * 2021-05-19 2022-11-24 Albert Einstein College Of Medicine Single-component near-infrared optogenetic systems for gene transcription regulation

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1434852B1 (en) 2001-08-23 2007-04-25 The Regents of the University of California A universal light-switchable gene promoter system
US7795397B2 (en) * 2004-05-06 2010-09-14 The Regents Of The University Of California Red and near infrared flourescent phyotochrome
US20100093051A1 (en) 2006-10-27 2010-04-15 Kehoe David M Light regulated transcription system for use in prokaryotic organisms
US8828658B2 (en) 2008-05-23 2014-09-09 The Regents Of The University Of California Spatio-temporal control of protein interactions using phytochromes

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ARAI R ET AL: "Design of the linkers which effectively separate domains of a bifunctional fusion protein", PROTEIN ENGINEERING, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 14, no. 8, 1 August 2001 (2001-08-01), pages 529 - 532, XP002334380, ISSN: 0269-2139, DOI: 10.1093/PROTEIN/14.8.529 *
BARENDS THOMAS R M ET AL: "Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase.", NATURE 18 JUN 2009, vol. 459, no. 7249, 18 June 2009 (2009-06-18), pages 1015 - 1018, XP002734152, ISSN: 1476-4687 *
LOSI A ET AL: "Bacterial bilin- and flavin-binding photoreceptors.", PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES : OFFICIAL JOURNAL OF THE EUROPEAN PHOTOCHEMISTRY ASSOCIATION AND THE EUROPEAN SOCIETY FOR PHOTOBIOLOGY OCT 2008, vol. 7, no. 10, October 2008 (2008-10-01), pages 1168 - 1178, XP002734151, ISSN: 1474-905X *
RYU MIN-HYUNG ET AL: "Engineering adenylate cyclases regulated by near-infrared window light.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 15 JUL 2014, vol. 111, no. 28, 15 July 2014 (2014-07-15), pages 10167 - 10172, XP002734153, ISSN: 1091-6490 *
RYU MIN-HYUNG ET AL: "Near-infrared Light Responsive Synthetic c-di-GMP Module for Optogenetic Applications.", ACS SYNTHETIC BIOLOGY 21 NOV 2014, vol. 3, no. 11, 21 November 2014 (2014-11-21), pages 802 - 810, XP002734154, ISSN: 2161-5063 *
See also references of WO2013016693A2 *
SHU XIAOKUN ET AL: "Mammalian Expression of Infrared Fluorescent Proteins Engineered from a Bacterial Phytochrome", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, vol. 324, no. 5928, 8 May 2009 (2009-05-08), pages 804 - 807, XP001525403, ISSN: 0036-8075, DOI: 10.1126/SCIENCE.1168683 *

Also Published As

Publication number Publication date
WO2013016693A3 (en) 2013-04-04
EP2737062A2 (en) 2014-06-04
US20130030041A1 (en) 2013-01-31
EP2737062B1 (en) 2017-08-23
US8835399B2 (en) 2014-09-16
WO2013016693A2 (en) 2013-01-31
DK2737062T3 (en) 2017-12-04

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