EP2689006A4 - In situ expression of lipase for enzymatic production of alcohol esters during fermentation - Google Patents

In situ expression of lipase for enzymatic production of alcohol esters during fermentation

Info

Publication number
EP2689006A4
EP2689006A4 EP12760739.8A EP12760739A EP2689006A4 EP 2689006 A4 EP2689006 A4 EP 2689006A4 EP 12760739 A EP12760739 A EP 12760739A EP 2689006 A4 EP2689006 A4 EP 2689006A4
Authority
EP
European Patent Office
Prior art keywords
lipase
during fermentation
alcohol esters
enzymatic production
situ expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP12760739.8A
Other languages
German (de)
French (fr)
Other versions
EP2689006A2 (en
Inventor
Robert Dicosimo
Arthur Leo Kruckeberg
Aken Thomas Edwin Van
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Butamax Advanced Biofuels LLC
Original Assignee
Butamax Advanced Biofuels LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Butamax Advanced Biofuels LLC filed Critical Butamax Advanced Biofuels LLC
Publication of EP2689006A2 publication Critical patent/EP2689006A2/en
Publication of EP2689006A4 publication Critical patent/EP2689006A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)
EP12760739.8A 2011-03-23 2012-03-23 In situ expression of lipase for enzymatic production of alcohol esters during fermentation Withdrawn EP2689006A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161466712P 2011-03-23 2011-03-23
US201161498292P 2011-06-17 2011-06-17
PCT/US2012/030468 WO2012129548A2 (en) 2011-03-23 2012-03-23 In situ expression of lipase for enzymatic production of alcohol esters during fermentation

Publications (2)

Publication Number Publication Date
EP2689006A2 EP2689006A2 (en) 2014-01-29
EP2689006A4 true EP2689006A4 (en) 2014-10-01

Family

ID=46880081

Family Applications (1)

Application Number Title Priority Date Filing Date
EP12760739.8A Withdrawn EP2689006A4 (en) 2011-03-23 2012-03-23 In situ expression of lipase for enzymatic production of alcohol esters during fermentation

Country Status (8)

Country Link
EP (1) EP2689006A4 (en)
JP (1) JP2014509869A (en)
CN (1) CN103459587A (en)
AU (1) AU2012230730A1 (en)
BR (1) BR112013024329A2 (en)
CA (1) CA2829941A1 (en)
WO (1) WO2012129548A2 (en)
ZA (1) ZA201306647B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106399286A (en) * 2016-10-18 2017-02-15 江南大学 Method for promoting Pichia pastoris to produce alkaline pectinase

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9238828B2 (en) * 2011-07-28 2016-01-19 Butamax Advanced Biofuels Llc Keto-isovalerate decarboxylase enzymes and methods of use thereof
US20160138050A1 (en) * 2013-07-16 2016-05-19 Butamax Advanced Biofuels Llc Yeast with increased butanol tolerance involving cell wall proteins
CN107488646B (en) * 2016-06-10 2019-12-10 广东溢多利生物科技股份有限公司 Lipase mutant TTL-Arg59Ser/Gly60Glu/Ser61Asn/Ile62Val and gene and application thereof
CN107488644B (en) * 2016-06-10 2019-12-10 广东溢多利生物科技股份有限公司 Lipase TTL mutant TTL-Gly60Glu/Ser61Asn with improved thermal stability and gene and application thereof
CN107488648B (en) * 2016-06-10 2019-12-10 广东溢多利生物科技股份有限公司 Lipase TTL mutant TTL-Arg59Ser/Gly60Glu/Ser61Asn with improved thermal stability, gene and application
CN107488647B (en) * 2016-06-10 2019-12-10 广东溢多利生物科技股份有限公司 Lipase TTL mutant TTL-Gly60Glu with improved thermal stability and gene and application thereof
CN107488645B (en) * 2016-06-10 2019-12-10 广东溢多利生物科技股份有限公司 Lipase TTL mutant TTL-Ser61Asn with improved thermal stability and gene and application thereof
CN106701606B (en) * 2016-12-19 2021-03-26 广东利世康低碳科技有限公司 Genetic engineering candida utilis capable of degrading and utilizing kitchen waste and construction method thereof
US10604776B2 (en) 2017-05-24 2020-03-31 Poet Research, Inc. Use of an esterase to enhance ethyl ester content in fermentation media
CN108277212B (en) * 2017-12-29 2019-12-10 广东溢多利生物科技股份有限公司 Lipase mutant Gly183Cys/Gly212Cys and gene and application thereof
CN108315312B (en) * 2018-04-11 2019-12-10 广东溢多利生物科技股份有限公司 Lipase TTL mutant with improved thermal stability and coding gene and application thereof
WO2019241269A1 (en) 2018-06-11 2019-12-19 Poet Research, Inc. Methods of refining a grain oil composition feedstock, and related systems, compositions and uses
JPWO2021045234A1 (en) * 2019-09-06 2021-03-11
BR112023001818A2 (en) 2020-08-06 2023-02-23 Poet Res Inc ENDOGENOUS LIPASE FOR METAL REDUCTION IN CORN DISTILLERY OIL
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011063402A2 (en) * 2009-11-23 2011-05-26 Butamax(Tm) Advanced Biofuels Llc Method for producing butanol using extractive fermentation with osmolyte addition

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0789079B1 (en) * 1995-09-07 2002-06-12 Kansai Paint Co., Ltd. Coupling process of fermentation and microbial transformation reaction
US20040253696A1 (en) * 2003-06-10 2004-12-16 Novozymes North America, Inc. Fermentation processes and compositions
CA2678395C (en) * 2007-02-13 2016-06-28 Water Solutions, Inc. Process for improving the yield and efficiency of an ethanol fermentation plant
WO2009006386A2 (en) * 2007-06-29 2009-01-08 The Regents Of The University Of California Host cells and methods for producing isoprenyl alkanoates
BRPI0909965A2 (en) * 2008-06-04 2015-08-04 Butamax Advanced Biofuels Llc Method for the recovery of butanol from a fermentation medium and method for the production of butanol
EP2582823A1 (en) * 2010-06-18 2013-04-24 Butamax(tm) Advanced Biofuels LLC Supplementation of fatty acids for improving alcohol productivity

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011063402A2 (en) * 2009-11-23 2011-05-26 Butamax(Tm) Advanced Biofuels Llc Method for producing butanol using extractive fermentation with osmolyte addition

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
HUGHES ET AL: "Production of Candida antarctica lipase B gene open reading frame using automated PCR gene assembly protocol on robotic workcell and expression in an ethanologenic yeast for use as resin-bound biocatalyst in biodiesel production", JALA (JOURNAL OF LABORATORY AUTOMATION), vol. 16, February 2011 (2011-02-01), pages 17 - 37, XP028132864 *
OLIVEIRA ET AL: "In situ recovery of ethanol by extraction and enzymatic esterification", MEDEDELINGEN FACULTEIT LANDBOUWWETENSCHAPPEN RIJKSUNIVERSITEIT GENT, vol. 63/4a, 1998, pages 1231 - 1238, XP002683137 *
OLIVEIRA ET AL: "Production and extractive biocatalysis of ethanol using microencapsulated yeast cells and lipase systems", JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, vol. 52, 1991, pages 219 - 225, XP055029174 *
SRIMHAN ET AL: "Selection of lipase producing yeasts for methanol-tolerant biocatalyst as whole cell application for palm-oil transesterification", ENZYME AND MICROBIAL TECHNOLOGY, vol. 48, 7 March 2011 (2011-03-07), pages 293 - 298, XP028138965 *
YOSHIDA ET AL: "Water activity dependence of performance of surface-displayed lipase in yeast cells: A unique water requirement for enzymatic synthetic reaction in organic media", ENZYME AND MICROBIAL TECHNOLOGY, vol. 48, 7 April 2011 (2011-04-07), pages 334 - 338, XP028372461 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106399286A (en) * 2016-10-18 2017-02-15 江南大学 Method for promoting Pichia pastoris to produce alkaline pectinase
CN106399286B (en) * 2016-10-18 2019-12-24 江南大学 Method for promoting pichia pastoris to produce alkaline pectinase

Also Published As

Publication number Publication date
EP2689006A2 (en) 2014-01-29
AU2012230730A1 (en) 2013-09-19
JP2014509869A (en) 2014-04-24
CN103459587A (en) 2013-12-18
WO2012129548A2 (en) 2012-09-27
BR112013024329A2 (en) 2017-12-19
CA2829941A1 (en) 2012-09-27
ZA201306647B (en) 2014-11-26
WO2012129548A3 (en) 2013-03-14

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Inventor name: KRUCKEBERG, ARTHUR LEO

Inventor name: DICOSIMO, ROBERT

Inventor name: VAN AKEN, THOMAS EDWIN

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A4 Supplementary search report drawn up and despatched

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