EP2624954A1 - Verfahren zum waschen einer mikrofluidischen kavität - Google Patents
Verfahren zum waschen einer mikrofluidischen kavitätInfo
- Publication number
- EP2624954A1 EP2624954A1 EP11764212.4A EP11764212A EP2624954A1 EP 2624954 A1 EP2624954 A1 EP 2624954A1 EP 11764212 A EP11764212 A EP 11764212A EP 2624954 A1 EP2624954 A1 EP 2624954A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- cavity
- washing
- liquid
- gas
- chamber
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000005406 washing Methods 0.000 title claims abstract description 83
- 238000000034 method Methods 0.000 title claims abstract description 25
- 239000007788 liquid Substances 0.000 claims abstract description 90
- 230000004888 barrier function Effects 0.000 abstract description 3
- 239000007789 gas Substances 0.000 description 35
- 239000003570 air Substances 0.000 description 29
- 238000001514 detection method Methods 0.000 description 7
- 239000012530 fluid Substances 0.000 description 5
- 239000000523 sample Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 125000000524 functional group Chemical group 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000009792 diffusion process Methods 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 2
- 239000012080 ambient air Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 229920002477 rna polymer Polymers 0.000 description 2
- 238000004026 adhesive bonding Methods 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229910052756 noble gas Inorganic materials 0.000 description 1
- 150000002835 noble gases Chemical class 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000005201 scrubbing Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L13/00—Cleaning or rinsing apparatus
- B01L13/02—Cleaning or rinsing apparatus for receptacle or instruments
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/14—Process control and prevention of errors
- B01L2200/141—Preventing contamination, tampering
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T137/00—Fluid handling
- Y10T137/0318—Processes
- Y10T137/0402—Cleaning, repairing, or assembling
- Y10T137/0419—Fluid cleaning or flushing
- Y10T137/0424—Liquid cleaning or flushing
Definitions
- the invention relates to a method for washing a cavity in a microfluidic component.
- the invention also relates to a microfluidic component for carrying out such a method.
- a basic task of this technology is the detection of biological molecules such as DNA (deoxyribonucleic acid) or RNA (ribonucleic acid), proteins, polypeptides, etc.
- biological molecules such as DNA (deoxyribonucleic acid) or RNA (ribonucleic acid), proteins, polypeptides, etc.
- DNA deoxyribonucleic acid
- RNA ribonucleic acid
- proteins proteins
- polypeptides etc.
- genetic information is encoded, of particular interest.
- microfluidic components or microfluidic cartridges are increasingly being used.
- Microfluidic cartridges are widely used in the form of Einmaltests, which are usually used so-called lateral flow cartridges whose components have length and width measurements, which are a few millimeters to centimeters.
- a cartridge containing a biosensor is supplied with an analysis fluid (eg, blood, urine, or saliva) to be tested.
- the sample is added to the cartridge before or after inserting the cartridge into an analyzer.
- the addition of the analyte takes place in an opening in the cartridge, the liquid being supplied through microchannels to corresponding sample preparation chambers and to sample examination chambers.
- micro is intended to imply that the channels and / or cavities (chambers) have a dimension on the micrometer scale, at least in a geometric extension direction, i.e., the dimensions are less than one millimeter in at least one dimension.
- microfluidic is understood to mean that a pressure-induced and / or capillary fluid flow takes place through and in the microchannels and / or microcavities.
- microfluidic component is understood to mean a component which has at least such microchannels or microcavities for the storage and transport of liquids or fluids and gases.
- microfluidic cartridge is understood to mean an apparatus (possibly consisting of several microfluidic components) for the analysis of liquids.
- the detection of low concentrations of biological and inorganic substances in biological samples is often difficult.
- the assays for this type of detection in microfluidic cartridges are usually associated with several steps, which include binding a primary antibody, multiple washes, binding a second antibody, further washes, and (depending on the type of detection system ) optionally additionally include enzymatic and washing measures.
- microfluidic cartridges The number of steps typically required in the use of such microfluidic cartridges to obtain a desired, specific signal is time consuming and labor intensive.
- the demand with modern microfluidic cartridges aims at a shortening in the measuring time between the task of the sample liquid and finally the appearance of the measured value. This time is extended by frequent washing steps, but these are mostly desirable and necessary to increase sensitivity and reduce background levels.
- a Kam mer In egg nem washing a Kam mer usually a previously introduced into the chamber liquid (for example, reaction liquid) is washed out by a directly following introduced into the chamber washing liquid. Specifically, an amount of washing liquid is passed through the chamber, wherein the liquid to be washed from the chamber mixed with the washing liquid (diffusion) and is removed with the washing liquid from the chamber.
- a previously introduced into the chamber liquid for example, reaction liquid
- a directly following introduced into the chamber washing liquid Specifically, an amount of washing liquid is passed through the chamber, wherein the liquid to be washed from the chamber mixed with the washing liquid (diffusion) and is removed with the washing liquid from the chamber.
- washing process in a microfluidic system generally proceeds in the form of a laminar flow without significant turbulent content, the liquid to be washed away is not adequately detected by the washing liquid, particularly in the corner regions of chambers. As a result, residues remain in the chamber. This usually requires a multiple repetition of washing steps, which, however, is counterproductive in terms of achieving the shortest possible measuring time. In addition, this opens up the demand Washing liquid and thus the space required for reservoir and waste in the air, which is undesirable in a volume-minimized microfluidic system.
- the invention is based on the object to provide a generic method for washing a cavity in a microfluidic component, wherein the efficiency of the
- the invention is also based on the object to provide a microfluidic component for carrying out the method according to the invention.
- the invention is therefore based on a method for washing at least one cavity in a microfluidic component, wherein a first liquid is contained in the cavity and at least one second liquid is supplied to the cavity for washing.
- a gas is supplied to the cavity before supplying the washing liquid.
- pre-washing it is possible that the need for subsequently to be supplied washing liquid, which is necessary in order to bring about a respective desired reduction of the residual concentration of the liquid to be washed in the cavity, can be significantly reduced.
- the need for washing liquid can thus be reduced and under certain circumstances, a reduction of the washing time or of washing is possible.
- volume is passed through the cavity. This makes it possible to implement the method in a microfluidic component or a microfluidic cartridge without an external gas connection, so that, for example, the gas bubble with a defined volume can be provided in a cavity of the microfluidic component itself.
- the gas bubble has a volume which is smaller than the volume of the cavity.
- the volume is still sufficiently large to choose for efficient washing.
- the volume of the gas bubble in about 40% to 60%, preferably selected in about 50% of the volume of the cavity to be washed out. This significantly reduces the need for gas to be stored, yet is fully sufficient to achieve the desired functionality or effect.
- the gas bubble spreads when passing into the cavity to be washed by means of overpressure continuously and immediately becomes so wide that it touches the side walls of the cavity. Thus, it can displace a large part of the liquid in the cavity to be washed out through an outlet opening to be provided in the cavity. Subsequent washing liquid in turn displaces the gas bubble also in the direction of the outlet opening.
- the gas bubble works as a kind of barrier between the first,
- the washing liquid can readily absorb any residual, still small amount of liquid to be washed by diffusion and carry it out of the cavity on further transport. Under certain circumstances, therefore, even a single washing step to achieve a desired
- Residual concentration is sufficient.
- the invention also wishes to provide a microfluidic component for carrying out the method according to the invention.
- the invention is based on a microfluidic component comprising at least one first cavity which is filled with a liquid for washing at least one second cavity and means for establishing a fluidic connection between the at least one first and the at least one second cavity.
- at least one further cavity between the first and the second cavity, which is filled with a gas, is now arranged in the flow direction of the liquid.
- the washing liquid flows in the direction of the cavity containing the gas and, if necessary, presses the gas bubble before it is released into the cavity to be washed out after the release of a corresponding fluidic connection (for example by means of appropriate valves) ,
- the at least one further gas-filled cavity has a volume which is smaller than the volume of at least one second, to be washed cavity. Because it has been shown that even a significantly smaller gas volume than the volume of the cavity to be washed already sufficient to achieve the desired Wrkung.
- a control can be preferably by means of electrical signals or
- Input opening and the second portion may be arranged in the region of the outlet opening.
- the gas-filled cavity is fluidically connectable with at least one further gas reservoir.
- controllable valve may be provided controllable valve.
- microfluidic component or the microfluidic cartridge it is possible with the microfluidic component or the microfluidic cartridge to repeat the steps described (introduction of a gas bubble into the cavity to be washed - pushing out of the gas bubble by subsequent washing liquid) several times if necessary.
- air is also used here as the gas, it being possible for the ambient air to be used as a further gas reservoir.
- Fig. 1 is a principle plan view of a part of an inventive
- Microfluidic device according to a first embodiment
- Fig. 2 is a principle plan view of a part of an inventive
- Microfluidic device according to a second embodiment
- 3a is a schematic single representation of a cavity which is washed, in a first embodiment
- 3b shows a principle individual representation of a cavity, which with a
- washing liquid is washed, in a second embodiment
- FIG. 4 shows a basic representation of the method according to the invention using the example of a cavity according to FIG. 3b.
- FIG. 1 shows a detail of a microfluidic component 1.
- a microfluidic function group 90 (dashed lines bordered) to be assigned.
- the microfluidic functional group 90 comprises a first, preferably circular chamber 10 filled with washing liquid F 2.
- a second, approximately rectangular chamber 20 can be seen, which is filled with a liquid F1.
- the liquid F1 has triggered a certain detection reaction in the chamber 20. Part of the biomolecules contained in F1 is bound in the chamber 20. Now, the rest of F1 with the washing liquid F2 is to be washed out of the chamber 20.
- PCR Polymerase Chain Reaction
- the type of detection reaction produced in the chamber 20 by the liquid F1 is not relevant to the understanding of the invention and therefore need not be further explained.
- a further chamber 30 is arranged, which is filled in the embodiment with air L.
- air instead of air, of course, other gases, such as nitrogen or the like can be used.
- the chambers 10, 20 and 30 are fluidly interconnected by means of microchannels 40, wherein between the chambers 10 and 30 or 30 and 20 respectively a preferably electrically controllable valve 50a and 50b is provided, with which the fluidic connection is releasable or interruptible ,
- microchannel 80 is provided, with which the fluidic connection from the chamber 20 to other, not shown microfluidic
- Functional elements e.g. A waste area can be produced.
- the air-filled chamber 30 is connected to a microchannel 60.
- the microchannel 60 establishes a fluidic connection of the chamber 30 to a further gas reservoir.
- the fluidic connection can be interrupted or released by means of a preferably electrically controllable valve 70.
- the mentioned gas reservoir itself can be realized by one or more further cavities or chambers (not shown).
- the gas reservoir accessible via the microchannel 60 is filled with air or to provide access to the ambient air or to an air pump (not shown) via the microchannel 60.
- an air pump not shown
- Not shown in detail or numbered is connected to the component 1 preferably by gluing foil for capping or sealing of said chambers and channels.
- the component 1 itself is a plastic plate, which is preferably produced by injection molding.
- the chamber 10 is now applied in the application example with a pressure of about 0.4 bar to 0.8 bar. This is preferably done by means of suitable actuators of a microfluidic cartridge, in which the component 1 is installed (not shown).
- valve 50a is closed again for this purpose.
- the valve 70 is then opened and a fluidic connection between the chamber 30 to the mentioned air reservoir is released.
- the chamber 30 can be filled again with air L, for example by a pump.
- the valve 50a is opened again and pressure builds up on the chamber 10, as already described.
- the chamber 10 may be varied in size and shape as needed. Also, several chambers 10 are conceivable, which are each assigned to a washing step. The washing process in chamber 20 will be explained in more detail below in connection with FIG. 4.
- FIG. 2 shows in principle a further embodiment 1 'of a microfluidic component according to the invention.
- the microfluidic component 1 ' has a plurality of microfluidic functional groups 90 (as described in FIG. 1). Accordingly, several further micro-channels 80 are provided. They can, for example, be connected to a common waste area.
- This embodiment 1 'can serve, for example, to those in the functional groups 90th
- FIG. 3 two possible geometries of the chamber to be washed 20 are shown, of course, other geometries are conceivable.
- the chamber geometry according to FIG. 3b represents an improvement in terms of the washing efficiency compared to the geometry according to FIG. 3a and can advantageously be combined with the method according to the invention.
- Fig. 3a it can be seen that the chamber 20 as shown in Fig. 1, is formed. Thus, it has an approximately rectangular plan view in plan view, wherein the inlet
- Micro channel 40 and the outlet (micro channel 80) can be seen.
- the chamber 20 is already penetrated here in the flow direction S by washing liquid F2.
- Chamber geometry is shown in Fig. 3b.
- Adjoining the inlet opening 21 is a first section 23 with a continuously widening cross-section of the chamber 20 '. Concrete run in this section 23 in plan view, the opposite walls of the chamber 20 'V-shaped apart.
- the section 23 is followed by a section 24 of constant cross-section of the chamber 20 '. In this case, the opposite walls of the chamber 20 'thus extend approximately parallel.
- the section 24 in turn is followed by a section 25 in which the cross section of the chamber 20 'continuously decreases.
- the opposite walls of the chamber 20 ' run toward each other in the direction of the outlet opening 22 in a V-shaped manner towards one another.
- the chamber geometry is thereby optimized with regard to the flow course of the washing liquid F2. Nevertheless, even here certain residues of wegzu Repeatder liquid F1 in the corners are unavoidable.
- FIG. 4 now shows in detail how the process according to the invention leads to a significant improvement in the washing efficiency:
- the chamber 20 is initially filled with the liquid F1 to be washed away (FIG. 4a). After initiation of the washing process (as described above) is first by the
- washing fluid F2 previously driven air bubble L in the chamber 20 ', namely in the region of the inlet opening 21 is pressed (Fig. 4b), until the entire air bubble L has been pressed into the chamber 20' (Fig. 4c). It can be seen that the air bubble L spreads very quickly outward in the direction of the side walls of the chamber 20 'and forms with these contact areas 26.
- the size of the air bubble L by no means has to correspond to the volume of the chamber 20 '. It should only be ensured that the defined amount of air L in the chamber 30 is so large that an air bubble L can be generated which is so large that they form with the chamber 20 'the mentioned contact regions 26 and thus virtually as a barrier layer between the liquid F1 and can serve subsequent liquid F2.
- Fluid F2 has been displaced in the direction of the outlet opening 22, the liquid F1 has displaced to a very high percentage from the chamber 20 '.
- Liquid F2 is forced into the outlet opening 22 and finally in the chamber 20 'only the liquid F2 is located. If necessary, only a very small amount of liquid F1 to be washed away in the chamber 20 'must then diffuse with the washing liquid F 2 with the washing liquid F 2.
- a pressure of about 0.4 bar has proved to be extremely useful, but also significantly higher pressures to about 0.8 bar found application.
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Cleaning By Liquid Or Steam (AREA)
Abstract
Description
Claims
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP11764212.4A EP2624954B1 (de) | 2010-10-07 | 2011-10-04 | Verfahren zum waschen einer mikrofluidischen kavität |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP10186833 | 2010-10-07 | ||
PCT/EP2011/067341 WO2012045754A1 (de) | 2010-10-07 | 2011-10-04 | Verfahren zum waschen einer mikrofluidischen kavität |
EP11764212.4A EP2624954B1 (de) | 2010-10-07 | 2011-10-04 | Verfahren zum waschen einer mikrofluidischen kavität |
Publications (2)
Publication Number | Publication Date |
---|---|
EP2624954A1 true EP2624954A1 (de) | 2013-08-14 |
EP2624954B1 EP2624954B1 (de) | 2020-08-26 |
Family
ID=43754900
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP11764212.4A Active EP2624954B1 (de) | 2010-10-07 | 2011-10-04 | Verfahren zum waschen einer mikrofluidischen kavität |
Country Status (6)
Country | Link |
---|---|
US (1) | US9089883B2 (de) |
EP (1) | EP2624954B1 (de) |
JP (1) | JP6015659B2 (de) |
DK (1) | DK2624954T3 (de) |
ES (1) | ES2821373T3 (de) |
WO (1) | WO2012045754A1 (de) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10639628B2 (en) | 2013-12-20 | 2020-05-05 | 3M Innovative Properties Company | Systems and methods for sample concentration and detection |
US20190329240A1 (en) * | 2016-02-17 | 2019-10-31 | Hitachi High-Technologies Corporation | Analysis Apparatus |
US10046322B1 (en) | 2018-03-22 | 2018-08-14 | Talis Biomedical Corporation | Reaction well for assay device |
US11008627B2 (en) | 2019-08-15 | 2021-05-18 | Talis Biomedical Corporation | Diagnostic system |
Family Cites Families (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0623770B2 (ja) * | 1986-01-31 | 1994-03-30 | 日本電子株式会社 | 自動化学分析装置における反応管の洗浄方法 |
US8349602B1 (en) | 1996-04-19 | 2013-01-08 | Xenogen Corporation | Biodetectors targeted to specific ligands |
US6345642B1 (en) * | 1999-02-19 | 2002-02-12 | Applied Materials, Inc. | Method and apparatus for removing processing liquid from a processing liquid path |
JP2000271471A (ja) * | 1999-03-24 | 2000-10-03 | Nippon M K S Kk | 液体ソース供給システム及びその洗浄方法、気化器 |
GB2358791A (en) * | 2000-02-04 | 2001-08-08 | Versar Inc | Method composition and apparatus for cleaning internal surfaces of oxygen converters and cylinders |
US6450182B2 (en) * | 2000-04-12 | 2002-09-17 | Versar, Inc. | Methods, compositions and apparatus for cleaning pipes |
GB2365526B (en) | 2000-07-31 | 2003-12-03 | Cambridge Life Sciences | Assay apparatus for measuring the amount of an analyte in a biological or environmental sample |
TW590982B (en) * | 2002-09-27 | 2004-06-11 | Agnitio Science & Technology I | Micro-fluid driving device |
JP2005037368A (ja) * | 2003-05-12 | 2005-02-10 | Yokogawa Electric Corp | 化学反応用カートリッジおよびその作製方法および化学反応用カートリッジ駆動システム |
US8030057B2 (en) | 2004-01-26 | 2011-10-04 | President And Fellows Of Harvard College | Fluid delivery system and method |
PT1776181E (pt) * | 2004-01-26 | 2014-01-02 | Harvard College | Sistema e método para o fornecimento de fluidos |
US20050249641A1 (en) * | 2004-04-08 | 2005-11-10 | Boehringer Ingelheim Microparts Gmbh | Microstructured platform and method for manipulating a liquid |
JP2006272268A (ja) * | 2005-03-30 | 2006-10-12 | Fuji Photo Film Co Ltd | マイクロ化学装置の洗浄方法 |
JP4720419B2 (ja) * | 2005-10-11 | 2011-07-13 | 株式会社島津製作所 | マイクロチップへの分離バッファ液充填装置とそれを備えたマイクロチップ処理装置 |
TWI272464B (en) * | 2006-03-01 | 2007-02-01 | Instr Technology Res Ct Nat Ap | Fluid order controlling apparatus and method |
JP2007326181A (ja) * | 2006-06-08 | 2007-12-20 | Fuji Xerox Co Ltd | マイクロ流路の洗浄方法 |
US8594848B2 (en) * | 2006-11-28 | 2013-11-26 | Lester F. Ludwig | Reconfigurable chemical process systems |
US8226774B2 (en) * | 2008-09-30 | 2012-07-24 | Princeton Trade & Technology, Inc. | Method for cleaning passageways such an endoscope channels using flow of liquid and gas |
US20100143194A1 (en) * | 2008-12-08 | 2010-06-10 | Electronics And Telecommunications Research Institute | Microfluidic device |
-
2011
- 2011-10-04 JP JP2013532176A patent/JP6015659B2/ja active Active
- 2011-10-04 ES ES11764212T patent/ES2821373T3/es active Active
- 2011-10-04 WO PCT/EP2011/067341 patent/WO2012045754A1/de active Application Filing
- 2011-10-04 DK DK11764212.4T patent/DK2624954T3/da active
- 2011-10-04 EP EP11764212.4A patent/EP2624954B1/de active Active
- 2011-10-04 US US13/877,719 patent/US9089883B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
JP2013539044A (ja) | 2013-10-17 |
US20140150890A1 (en) | 2014-06-05 |
US9089883B2 (en) | 2015-07-28 |
EP2624954B1 (de) | 2020-08-26 |
DK2624954T3 (da) | 2020-11-23 |
ES2821373T3 (es) | 2021-04-26 |
JP6015659B2 (ja) | 2016-10-26 |
WO2012045754A1 (de) | 2012-04-12 |
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