EP2405959A4 - Procédés, appareils et trousses pour introduire du matériel génétique dans des cellules vivantes - Google Patents

Procédés, appareils et trousses pour introduire du matériel génétique dans des cellules vivantes

Info

Publication number
EP2405959A4
EP2405959A4 EP10751463.0A EP10751463A EP2405959A4 EP 2405959 A4 EP2405959 A4 EP 2405959A4 EP 10751463 A EP10751463 A EP 10751463A EP 2405959 A4 EP2405959 A4 EP 2405959A4
Authority
EP
European Patent Office
Prior art keywords
kits
apparatuses
methods
genetic material
living cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP10751463.0A
Other languages
German (de)
English (en)
Other versions
EP2405959A1 (fr
Inventor
Thomas Diefenbach
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tufts University
Original Assignee
Tufts University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tufts University filed Critical Tufts University
Publication of EP2405959A1 publication Critical patent/EP2405959A1/fr
Publication of EP2405959A4 publication Critical patent/EP2405959A4/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
EP10751463.0A 2009-03-13 2010-03-12 Procédés, appareils et trousses pour introduire du matériel génétique dans des cellules vivantes Pending EP2405959A4 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15985609P 2009-03-13 2009-03-13
PCT/US2010/027104 WO2010105135A1 (fr) 2009-03-13 2010-03-12 Procédés, appareils et trousses pour introduire du matériel génétique dans des cellules vivantes

Publications (2)

Publication Number Publication Date
EP2405959A1 EP2405959A1 (fr) 2012-01-18
EP2405959A4 true EP2405959A4 (fr) 2013-10-16

Family

ID=42728810

Family Applications (1)

Application Number Title Priority Date Filing Date
EP10751463.0A Pending EP2405959A4 (fr) 2009-03-13 2010-03-12 Procédés, appareils et trousses pour introduire du matériel génétique dans des cellules vivantes

Country Status (2)

Country Link
EP (1) EP2405959A4 (fr)
WO (1) WO2010105135A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9017991B2 (en) * 2009-03-13 2015-04-28 Tufts University Methods tip assemblies and kits for introducing material into cells
RS59898B1 (sr) 2011-10-17 2020-03-31 Massachusetts Inst Technology Intraćelijsko davanje
AU2014306423B2 (en) 2013-08-16 2019-04-18 Massachusetts Institute Of Technology Selective delivery of material to cells
CN107109362A (zh) 2014-10-31 2017-08-29 麻省理工学院 递送生物分子至免疫细胞
US10526573B2 (en) 2014-11-14 2020-01-07 Massachusetts Institute Of Technology Disruption and field enabled delivery of compounds and compositions into cells
JP7278027B2 (ja) 2015-01-12 2023-05-19 マサチューセッツ インスティテュート オブ テクノロジー マイクロ流体送達による遺伝子編集
EP3320082B1 (fr) 2015-07-09 2023-05-24 Massachusetts Institute of Technology Administration de substances à des cellules anucléées

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5922687A (en) * 1995-05-04 1999-07-13 Board Of Trustees Of The Leland Stanford Junior University Intracellular delivery of nucleic acids using pressure
EP0998945A1 (fr) * 1998-09-30 2000-05-10 Transgene S.A. Utilisation du magnésium (mg2+) pour l'amélioration de la déliverence des gènes dans la thérapie génique
WO2003061831A2 (fr) * 2002-01-25 2003-07-31 Dormann Joerg Martin Dispositif de prelevement d'echantillons et procede de determination quantifiable simultanee de germes infectieux
DE10361917A1 (de) * 2003-12-22 2005-07-21 Novosom Ag Verfahren zur Transfektion von Zellen
US20070077306A1 (en) * 2003-12-26 2007-04-05 Toshihiro Akaike Apatite particle, method of producing the same, apatite particle-gene complex, and method of gene transfection
DE102007004856A1 (de) * 2007-01-31 2008-08-07 Universität Wien Pipetteneinrichtung, Manipulationseinrichtung und Verfahren zur Manipulation biologischer Zellen

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6982063B2 (en) * 2001-05-25 2006-01-03 Matrix Technologies Corp Automated pipetting system

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5922687A (en) * 1995-05-04 1999-07-13 Board Of Trustees Of The Leland Stanford Junior University Intracellular delivery of nucleic acids using pressure
EP0998945A1 (fr) * 1998-09-30 2000-05-10 Transgene S.A. Utilisation du magnésium (mg2+) pour l'amélioration de la déliverence des gènes dans la thérapie génique
WO2003061831A2 (fr) * 2002-01-25 2003-07-31 Dormann Joerg Martin Dispositif de prelevement d'echantillons et procede de determination quantifiable simultanee de germes infectieux
DE10361917A1 (de) * 2003-12-22 2005-07-21 Novosom Ag Verfahren zur Transfektion von Zellen
US20070077306A1 (en) * 2003-12-26 2007-04-05 Toshihiro Akaike Apatite particle, method of producing the same, apatite particle-gene complex, and method of gene transfection
DE102007004856A1 (de) * 2007-01-31 2008-08-07 Universität Wien Pipetteneinrichtung, Manipulationseinrichtung und Verfahren zur Manipulation biologischer Zellen

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2010105135A1 *

Also Published As

Publication number Publication date
WO2010105135A1 (fr) 2010-09-16
EP2405959A1 (fr) 2012-01-18

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