EP2335830B2 - Centrifugeuse de laboratoire dotée d'un refroidissement par compresseur - Google Patents
Centrifugeuse de laboratoire dotée d'un refroidissement par compresseur Download PDFInfo
- Publication number
- EP2335830B2 EP2335830B2 EP09015628.2A EP09015628A EP2335830B2 EP 2335830 B2 EP2335830 B2 EP 2335830B2 EP 09015628 A EP09015628 A EP 09015628A EP 2335830 B2 EP2335830 B2 EP 2335830B2
- Authority
- EP
- European Patent Office
- Prior art keywords
- centrifuge
- compressor
- laboratory
- laboratory centrifuge
- compressors
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000001816 cooling Methods 0.000 claims description 8
- 238000009792 diffusion process Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 238000009434 installation Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000012141 concentrate Substances 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 239000013641 positive control Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000006835 compression Effects 0.000 description 2
- 238000007906 compression Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000005204 segregation Methods 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 230000008033 biological extinction Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B15/00—Other accessories for centrifuges
- B04B15/02—Other accessories for centrifuges for cooling, heating, or heat insulating
Definitions
- the present invention relates to a laboratory centrifuge according to the preamble of claim 1.
- Laboratory centrifuges of this type are used in biological, chemical and medical laboratories for separating various components in a liquid or for separating solids from a liquid. For this purpose, the centrifugal force, which has different effects with different masses, is used.
- centrifuges In currently used laboratory centrifuges, rotation speeds of up to 16,000 revolutions per minute are usually generated, whereby forces of up to approx. 21 x 9.81 m / s 2 are achieved. However, it has been shown that in such centrifuges no complete or no satisfactory separation of the sample liquid can be achieved. Centrifuges are currently being planned in which segregation is to be improved through higher rotation speeds of up to 25,000 revolutions per minute.
- the heat generated in this way causes the centrifuged samples to heat up strongly, which can easily lead to their destruction or uselessness.
- the samples usually have to be kept at defined temperatures, for example at temperatures of 4 ° C, 22 ° C or 37 ° C depending on the application.
- the object of the present invention is to provide laboratory centrifuges which enable better separation, that is to say a higher separation rate, of the centrifuged samples.
- the inventors have surprisingly recognized that the demixing rates of the centrifuged samples can be increased by using rotary compressors for active cooling within the laboratory centrifuge.
- rotary piston compressors i.e. compressors in which the compressor is constructed in such a way that the compressor body also executes at least a rotational movement in the compressor chamber, such vibrations can be significantly reduced because a rotational movement is always carried out inside the compressor, so that not in the the same extent as with linear compressors to overcome dead centers.
- Such compressors are already known from refrigerator construction, but these devices have completely different specifications than laboratory centrifuges.
- laboratory centrifuges are very small compared to refrigerators, which means that all components have to be accommodated in a very limited installation space:
- laboratory centrifuges with the very fast moving rotor have a moving part which, despite this limited installation space, must be able to be operated as unaffected by other components as possible .
- laboratory centrifuges have to cover a very wide temperature spectrum, and frequent temperature changes with high lowering speeds have to be implemented.
- rotary compressors are only now available which, with the same dimensions, allow at least the same compression power as a reciprocating compressor, so that the dimensions of the laboratory centrifuge do not increase when they are used.
- the starting currents can also advantageously be reduced with the specified rotary piston compressors.
- certain laboratory centrifuges have been allowed in some countries such as the USA are not sold due to the 110 V alternating current voltage network used there, as the current peaks generated by powerful reciprocating compressors when starting up would endanger the stability of the electricity networks commonly used in these countries.
- certain controls had to be provided in the laboratory centrifuges, which ensure that the compressor is only activated in such a way that the starting currents do not exceed the legally required values.
- the rotary piston compressors provided according to the invention, such controls can be dispensed with, so that the laboratory centrifuges are simply constructed and therefore more robust and cost-effective and their sale is also permitted in these countries.
- the rotary piston compressor is an electrically driven compressor (direct and / or alternating current).
- Such compressors can be built very compact and high-torque and their control is i.a. possible via a regulated switched-mode power supply or frequency converter independent of the mains voltage.
- such compressors also make it easier to achieve smaller performance levels.
- the compressors can be designed to be smaller and with less power overall, so that the installation space that can be used in a laboratory centrifuge can be used more effectively, as a result of which the overall volume required for such a laboratory centrifuge is reduced.
- the laboratory centrifuge according to the invention can be designed particularly advantageously as a bench-top laboratory centrifuge and microliter centrifuge and the like, since a very compact design is particularly important in these types of centrifuge.
- the laboratory centrifuge has a backmixing rate of less than or equal to 20%, preferably less than or equal to 17%, in particular less than or equal to 14%. Then the segregation rate is particularly high.
- FIG. 1 a laboratory centrifuge 1 which is not claimed is shown purely schematically.
- This laboratory centrifuge 1 has a housing 2 and a centrifuge lid 3.
- the centrifuge lid 3 is designed to close a centrifuge container 4 in which a rotor 5 is arranged.
- the rotor 5 can be driven by a motor (not shown), as a result of which samples (not shown) arranged on the rotor 5 can be centrifuged in order to separate them.
- the laboratory centrifuge 1 has an active cooling device which comprises a compressor 6.
- the compressor 6 is designed as a rotary compressor and has a rotary piston compressor. In the area of the compressor 6, the top of the housing 2 is not shown.
- This compressor 6 is very compact and high-torque and its regulation is possible via a regulated switched-mode power supply independent of the mains voltage. With the help of this compressor 6, smaller power gradations of the cooling power can also be easily made possible.
- FIG 2 a laboratory centrifuge 10 is shown purely schematically, as it is the prior art.
- This laboratory centrifuge 10 differs from the laboratory centrifuge 1 in that a compressor 11 with a reciprocating piston compressor is used as the compressor 11. All other parts are therefore provided with the same reference numerals as in the laboratory centrifuge 1.
- a comparative test of the backmixing rates of the laboratory centrifuge 1 in comparison to the conventional laboratory centrifuge 10 with a reciprocating compressor will now be described below.
- a laboratory centrifuge 1 with the XB357 rotary piston compressor from Mitsubishi was used, and the rotor F-45-24-11 from Eppendorf was used as the rotor 5.
- the centrifuge 5415 R from Eppendorf (type SN 5426 0023218) was used as laboratory centrifuge 10, which had a reciprocating compressor PL50 from Danfoss and the same rotor F-45-24-11 was used as rotor 5.
- the Eppendorf Reference 500 - 2500 ⁇ l pipette (model SN 475116) and the Eppendorf Research pipette per 5 - 100 ⁇ l (model SN 022760) were used.
- an Eppendorf biophotometer (type SN 6131 00197) was used.
- the samples were placed in 2.0 ml Safe-Lock vessels (model U123342P 2243) and a 10 mM Tris solution and a salt concentrate color solution with a density of 1.2 g / ml were used to prepare the samples.
- 1450 ⁇ l of Tris solution were pipetted into a 2.0 ml Safe-Lock vessel with the Eppendorf Reference pipette.
- 50 ⁇ l of the salt concentrate color solution were then placed under the layer, the pipette being set to the lowest aspiration and dilution level.
- the 50 ⁇ l layered salt concentrate color solution were removed from the vessels with the Eppendorf Research pipette. The vessels were then closed again and vigorously mixed. The liquid contained in the vessels was then transferred to a cuvette and measured photometrically at an extinction of 562 nm. The values obtained with the vessels of the positive control serve as maximum values (100% value) and the values obtained with the negative control serve as background (diffusion).
- Backmix rate centrifuged value - Diffusion value ⁇ 100 / 100 % - value .
- the laboratory centrifuge 1 can ensure significantly better demixing rates for centrifuged samples, since the provision according to the invention of at least one rotary compressor 6 means that significantly fewer vibrations are introduced into the laboratory centrifuge 1, resulting in significantly lower backmixing rates.
Landscapes
- Centrifugal Separators (AREA)
Claims (4)
- Centrifugeuse de laboratoire (1) comprenant un rotor (5) entraîné par un moteur de centrifugeuse, et un dispositif de refroidissement qui présente un compresseur (6), ledit compresseur étant un compresseur à rotation (6), caractérisée en ce que le compresseur à rotation présente un compresseur rotatif, au moins deux compresseurs étant disposés en parallèle.
- Centrifugeuse de laboratoire (1) selon la revendication 1, caractérisée en ce que le compresseur à rotation est un compresseur (6) entraîné électriquement, en particulier entraîné par une tension de courant continu et/ou par une tension de courant alternatif.
- Centrifugeuse de laboratoire (1) selon l'une quelconque des revendications précédentes, caractérisée en ce qu'il s'agit d'une centrifugeuse de laboratoire (1) de table, d'une centrifugeuse microlitre ou similaire.
- Centrifugeuse de laboratoire (1) selon l'une quelconque des revendications précédentes, caractérisée en ce que le taux de rétromélange est inférieur ou égal à 20 %, de préférence inférieur ou égal à 17 %, en particulier inférieur ou égal à 14 %.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP09015628.2A EP2335830B2 (fr) | 2009-12-17 | 2009-12-17 | Centrifugeuse de laboratoire dotée d'un refroidissement par compresseur |
CN201010595829.2A CN102166546B (zh) | 2009-12-17 | 2010-12-16 | 采用压缩机冷却的实验室离心机 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP09015628.2A EP2335830B2 (fr) | 2009-12-17 | 2009-12-17 | Centrifugeuse de laboratoire dotée d'un refroidissement par compresseur |
Publications (3)
Publication Number | Publication Date |
---|---|
EP2335830A1 EP2335830A1 (fr) | 2011-06-22 |
EP2335830B1 EP2335830B1 (fr) | 2013-03-27 |
EP2335830B2 true EP2335830B2 (fr) | 2020-11-11 |
Family
ID=42133502
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP09015628.2A Active EP2335830B2 (fr) | 2009-12-17 | 2009-12-17 | Centrifugeuse de laboratoire dotée d'un refroidissement par compresseur |
Country Status (2)
Country | Link |
---|---|
EP (1) | EP2335830B2 (fr) |
CN (1) | CN102166546B (fr) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE202013004850U1 (de) * | 2013-05-27 | 2013-06-05 | Thermo Electron Led Gmbh | Laborzentrifuge mit gedämmtem Kompressor |
DE102014107294B4 (de) * | 2014-05-23 | 2017-02-09 | Andreas Hettich Gmbh & Co. Kg | Zentrifuge |
DE102020119438A1 (de) | 2020-07-23 | 2022-01-27 | Andreas Hettich Gmbh & Co. Kg | Zentrifuge |
Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CH180152A (de) † | 1952-01-18 | 1935-10-15 | Bosch Robert Ag | Rollkolbenverdichter. |
DE2707145A1 (de) † | 1976-02-23 | 1977-09-01 | Beckman Instruments Inc | Zentrifuge |
JPH05228400A (ja) † | 1992-02-19 | 1993-09-07 | Hitachi Koki Co Ltd | 遠心機の冷却制御装置 |
DE4320537A1 (de) † | 1992-06-22 | 1993-12-23 | Mitsubishi Electric Corp | Gekapselter Rotationskompressor |
DE69310996T2 (de) † | 1992-04-29 | 1997-11-27 | Carrier Corp | Drehkolbenverdichter |
DE19932721C1 (de) † | 1999-07-16 | 2001-01-18 | Eppendorf Geraetebau Netheler | Laborzentrifuge mit Kühlaggregat |
US6993932B2 (en) † | 2003-12-26 | 2006-02-07 | Samsung Electronics Co., Ltd. | Refrigerant cycle system |
US20060210418A1 (en) † | 2003-06-11 | 2006-09-21 | Bae Ji Y | Rotary compressor |
US20070160486A1 (en) † | 2003-06-11 | 2007-07-12 | Ha Sam C | Rotary compressor |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2816449C3 (de) * | 1978-04-15 | 1981-05-27 | Heraeus-Christ Gmbh, 3360 Osterode | Zentrifuge, insbesondere Laborzentrifuge mit gekühlter Rotorkammer |
JPS592847Y2 (ja) * | 1979-07-18 | 1984-01-26 | 日立工機株式会社 | 遠心分離機 |
JP3596306B2 (ja) * | 1998-09-25 | 2004-12-02 | 日立工機株式会社 | 遠心分離機 |
JP2005230746A (ja) * | 2004-02-20 | 2005-09-02 | Hitachi Koki Co Ltd | 遠心分離機 |
CN2844510Y (zh) * | 2005-10-21 | 2006-12-06 | 乐金电子(天津)电器有限公司 | 旋转式压缩机 |
TWI414364B (zh) * | 2007-12-21 | 2013-11-11 | Alfa Wassermann Inc | 連續流超離心系統 |
CN201216967Y (zh) * | 2008-06-20 | 2009-04-08 | 上海力申科学仪器有限公司 | 离心通风降温结构 |
CN101455999B (zh) * | 2008-12-26 | 2012-10-31 | 上海力申科学仪器有限公司 | 一种台式高速离心机 |
-
2009
- 2009-12-17 EP EP09015628.2A patent/EP2335830B2/fr active Active
-
2010
- 2010-12-16 CN CN201010595829.2A patent/CN102166546B/zh active Active
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CH180152A (de) † | 1952-01-18 | 1935-10-15 | Bosch Robert Ag | Rollkolbenverdichter. |
DE2707145A1 (de) † | 1976-02-23 | 1977-09-01 | Beckman Instruments Inc | Zentrifuge |
JPH05228400A (ja) † | 1992-02-19 | 1993-09-07 | Hitachi Koki Co Ltd | 遠心機の冷却制御装置 |
DE69310996T2 (de) † | 1992-04-29 | 1997-11-27 | Carrier Corp | Drehkolbenverdichter |
DE4320537A1 (de) † | 1992-06-22 | 1993-12-23 | Mitsubishi Electric Corp | Gekapselter Rotationskompressor |
DE19932721C1 (de) † | 1999-07-16 | 2001-01-18 | Eppendorf Geraetebau Netheler | Laborzentrifuge mit Kühlaggregat |
US20060210418A1 (en) † | 2003-06-11 | 2006-09-21 | Bae Ji Y | Rotary compressor |
US20070160486A1 (en) † | 2003-06-11 | 2007-07-12 | Ha Sam C | Rotary compressor |
US6993932B2 (en) † | 2003-12-26 | 2006-02-07 | Samsung Electronics Co., Ltd. | Refrigerant cycle system |
Non-Patent Citations (5)
Title |
---|
Air Conditioning and Refrigeration Journal, " Design and Application of Small Screw Compressors - Part 2", Hermann Renz; Juli - September 2000 † |
Bitzer Letter, "Kälte-Klima-Aktuell (KKA)" † |
Broshüre Danfoss Hubkolbenverdichter † |
Imre Sallay et al: Journal of Hematotherapy & Stem Cell Research, Bd. 9, S. 877-883 (2000) † |
Product catalogue from Mitsubishi Electric, "Refrigerant Compressors Scroll/Rotary", Juli 2006 † |
Also Published As
Publication number | Publication date |
---|---|
CN102166546B (zh) | 2015-11-25 |
EP2335830B1 (fr) | 2013-03-27 |
CN102166546A (zh) | 2011-08-31 |
EP2335830A1 (fr) | 2011-06-22 |
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