EP2292329B1 - Substrat polymère doté d'une structure fluorescente, son procédé de fabrication et son utilisation - Google Patents

Substrat polymère doté d'une structure fluorescente, son procédé de fabrication et son utilisation Download PDF

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Publication number
EP2292329B1
EP2292329B1 EP09011507.2A EP09011507A EP2292329B1 EP 2292329 B1 EP2292329 B1 EP 2292329B1 EP 09011507 A EP09011507 A EP 09011507A EP 2292329 B1 EP2292329 B1 EP 2292329B1
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EP
European Patent Office
Prior art keywords
polymer substrate
fluorescent
sample chamber
cover plate
substrate according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Application number
EP09011507.2A
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German (de)
English (en)
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EP2292329A1 (fr
EP2292329A9 (fr
Inventor
Thomas Fischer
Joachim Stumpe
Valentin Kahl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV
Ibidi GmbH
Original Assignee
Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV
Ibidi GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV, Ibidi GmbH filed Critical Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung eV
Priority to EP09011507.2A priority Critical patent/EP2292329B1/fr
Priority to US12/876,881 priority patent/US9597688B2/en
Publication of EP2292329A1 publication Critical patent/EP2292329A1/fr
Publication of EP2292329A9 publication Critical patent/EP2292329A9/fr
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Publication of EP2292329B1 publication Critical patent/EP2292329B1/fr
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/54—Labware with identification means
    • B01L3/545—Labware with identification means for laboratory containers

Definitions

  • the invention relates to polymer substrates provided with fluorescence features in which fluorescence characteristics, ie fluorescent structures, are produced photochemically by UV irradiation.
  • fluorescence characteristics ie fluorescent structures
  • suitable fluorophors can be produced by suitable UV irradiation, which exhibit marked and detectable emission upon excitation with light of suitable wavelength. If such irradiation is carried out in a structured manner, emission patterns can be generated in this way in polymer substrates, which can be used, for example, as a recovery grid in fluorescence microscopy.
  • Another field of application relates to product authentication, which is made possible by the polymer substrates provided with fluorescence features according to the invention.
  • sample chambers In the field of culturing cells, a wide variety of sample chambers are used. These are mostly polymer-based and range from the cell culture bottle to slides and ⁇ -slides from ibidi to multiwell plates.
  • sample chambers can have contiguous areas on which cells grow from 1 mm 2 to 100 cm 2 . Since cells typically have a diameter of 1 .mu.m to 30 .mu.m, finding individual cells in large-area chambers without recovery structures is almost impossible.
  • the EP 2 008 715 A1 also describes a recovery grid, which is formed as part of the plastic body or is introduced into the plastic body.
  • the systems described here are based on non-fluorescent recovery gratings.
  • Another area of the prior art underlying the present invention relates to the authentication of products, especially consumer products.
  • fluorescence films can be structured by bleaching existing autofluorescence.
  • the FR 2 755902 A1 relates to a method for producing a labeled product as an integral component, wherein the label is caused by a fluorescent structure.
  • FR 2 909 922 A1 For example, a method is known for marking various materials, such as metal, plastic or ceramic, for example, wherein the marking is generated by laser bombardment in the picosecond range.
  • the DE 42 41 663 A1 relates to a method of marking an article, wherein the article is provided with a bar code which is readable by means of visible or UV light.
  • the preferred polymer substrates are low intrinsic fluorescence polymers, i. with an intrinsic fluorescence on the order of a cover glass in the excitation and emission range of 200 nm to 1000 nm. These include in particular COC, COP, PMMA, aliphatic polyesters and polyurethanes and polyethers. Particularly good polymers are suitable without UV stabilizers. For optical microscopy, in particular polymers with a refractive index between 1.4 and 1.6, in particular with 1.51 and / or a Abbe number over 50 and / or with a low birefringence are suitable.
  • a fluorescent structure is understood to mean structures up to the optical resolution limit. Typically, however, structures with a lateral resolution of several ⁇ m are used.
  • the fluorescent structure should have at least twice as high intensity as the non-fluorescent structure.
  • the structure should have such a high intensity that it can be easily recognized even at exposure times of less than one second with commercial fluorescence microscopes. This is already the case with a factor of 10.
  • the generated fluorophores the form the fluorescent structure, firmly bound in the polymer substrate and form a unit with it.
  • the fluorescent structures are thus an integral part of the carrier.
  • the modified polymer is characterized by the fact that both the fluorescent regions and the non-fluorescent regions consist of the identical original material. Therefore, the fluorophores also can not diffuse or sweat out.
  • These properties clearly delimit the structures produced, for example, from a printed, otherwise applied structure (eg an embossed or lasered structure) or dye-doped polymer systems.
  • no, possibly cell toxic, fluorescent dyes have to be used, which are applied to the polymer.
  • a structuring of the polymer substrate with fluorescent regions is produced.
  • a mask or a locally positionable radiation source is preferably used. If the UV irradiation is carried out in a structured manner, only the areas that have been irradiated will fluoresce significantly. In the non-irradiated areas, however, no substantial increase in the base emission is achieved. In this way, patterns can be generated which become visible upon excitation with light of suitable wavelength.
  • Such a photochemical structuring produces patterned fluorescent areas that are long-term stable and stable to environmental influences. Furthermore, they are characterized by the fact that they do not lose their edge sharpness by diffusion or similar processes. In addition, the features thus produced can not be removed without trace.
  • the fluorescent structure in the polymer substrate consists of several elements in the form of bars, lines, characters, figures, interference patterns, or combinations thereof.
  • fluorescent structures can be generated with only limited by the substrate size limited overall extent of the pattern.
  • the lateral resolution of the elements of the fluorescent structure is limited only by the method of introduction and the wavelength of the light.
  • the individual structural elements of the fluorescence pattern typically have a lateral extent of less than 100 ⁇ m, preferably less than 10 ⁇ m.
  • a method is also provided for producing the polymer substrate described above, in which a polymer substrate is at least partially subjected to irradiation in the wavelength range below 300 nm to produce fluorescent structures.
  • the preparation of the fluorescent structures by UV irradiation using masks by imaging or contact exposure takes place.
  • plates which have an exemplary variation of regions that are either transparent or non-transparent to the irradiation light used.
  • both radiation sources with coherent (laser direct writing) and focused beam (sample positioning) can be used for structuring.
  • Radiation sources used are preferably radiation sources with emission wavelengths or emission wavelength ranges in the range below 300 nm. These include, in particular, deuterium lamps, excimer lamps (Xe), excimer lasers (F 2 , ArF, KrF) or solid-state lasers (Nd: YVO 4 / YLF).
  • the polymer substrate used is preferably a polymer film.
  • the UV-generated generation of the fluorophores can be monitored in the absorption spectrum of the polymer substrate by an increase in the extinction in the UV range. However, these changes do not significantly affect the optical transmissivity in the visible region of the light to the human eye.
  • the polymer substrates can be provided without damage on the front or back with fluorescent features. Their production requires no exclusion of atmospheric oxygen.
  • the fluorescent features are further characterized by the fact that they are generated directly in materials that are used anyway for products from different areas. There are no additional coatings, imprints or other application fluorophores, fluorescent labels or their precursors. The fact that the features are produced directly in the material used, makes it much easier and cheaper to manufacture this compared to other methods. In addition, it is a fully optical process that does not require any wet-chemical development processes.
  • Such emission patterns can be used to provide substrates for fluorescence microscopy with a recovery grid.
  • the recovery grids are designed as a grid, which is also provided with numbers or letters. These recovery grids facilitate the retrieval of certain sample areas by being precisely charac- terised by the grid grid.
  • the recovery grids do not significantly affect biological processes and are sufficiently stable to perform longer observations on biological and other samples with greater reliability and reproducibility.
  • the described, photochemically generated characteristics are very well suited for typical excitation conditions in fluorescence microscopy; they are visible in all fluorescence channels (blue, green, yellow and red).
  • the lateral resolution in such produced features is sufficiently high, e.g. to allow a mapped observation of cells of different types.
  • Chambers in which such gratings can be introduced are in the DE 100 04 135 , of the DE 101 05 711 , of the EP 02 777 215 , of the EP 05 041 563 , of the EP 06 015 167 , of the EP 07 012 400 and the EP 09 006 487 described.
  • the upper part has at least one recess.
  • By connecting to the lower part of a reservoir is formed.
  • the recess of the upper part of the reservoir is then designed as a closed channel / tube or as an open-topped container.
  • the bottom formed by the base may be a foil or a coated glass carrier.
  • the bottom has a preferred thickness of 50 .mu.m to 250 .mu.m and / or an Abbe number greater than 50 and / or a refractive index between 1.2 and 1.8, in particular between 1.45 and 1.55. These properties are particularly well suited for (high-resolution) microscopy.
  • the bottom or film can be irradiated both from the side on which the cells are to be cultivated later and from the corresponding opposite side in order to produce fluorescent structures.
  • microfluidic analysis chambers can be provided with corresponding features.
  • coated glass supports can also be used.
  • Glass cover glasses are coated with a COP or COC layer to then introduce into this layer via the method described fluorescent grating.
  • the layer may e.g. be applied by spincoating etc.
  • labels, labels and products made of polymers can be provided with a feature by the structured UV irradiation, which becomes clearly visible only by excitation with light of suitable wavelength and intensity.
  • the counterfeiting security of the product increases and a non-erasable product individualization can be carried out, for example via barcodes.
  • This can be realized by a laser marking system, wherein the feature can also be read electronically.
  • Fig. 1 shows an emission spectrum of a polymer substrate according to the invention before and after the generation of fluorescence regions.
  • the irradiation for the generation of the fluorescence regions took place here with an ArF excimer laser.
  • the irradiation time was 10 seconds.
  • a polymer substrate according to the invention which has number patterns which were produced with the aid of a mask.
  • This is a fluorescence micrograph under excitation at a wavelength of 365 nm and 200x magnification.
  • a mask pattern chrome on silica glass
  • the mask rests with the chrome side. It is irradiated with a 30W deuterium lamp for 3 hours. Then the mask is removed and the emission pattern can be visualized by excitation at 365 nm.
  • a mask pattern chrome on silica glass
  • the mask rests with the chrome side. It is irradiated with an ArF excimer laser (193 nm) for 10 seconds. The mask is then removed and the emission pattern can be visualized by excitation at 365 nm, 436 nm or 515 nm.

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  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Claims (28)

  1. Substrat polymère ayant une structure fluorescente en tant que constituant intégral, la structure fluorescente étant une grille de récupération produite par voie photochimique.
  2. Substrat polymère selon la revendication 1, caractérisé en ce que le substrat polymère avant le traitement photochimique, et les matériaux de départ pour la fabrication du substrat polymère, ne contiennent sensiblement aucun fluorophore ou précurseur de ce dernier.
  3. Substrat polymère selon la revendication 2, caractérisé en ce que le substrat polymère comprend des domaines fluorescents et des domaines non fluorescents, les domaines fluorescents et les domaines non fluorescents du substrat polymère étant constitués de matériaux de départ identiques.
  4. Substrat polymère selon l'une des revendications précédentes, caractérisé en ce que l'intensité de l'émission des domaines fluorescents est deux fois, en particulier 10 fois plus élevée que l'intensité de l'émission des domaines non fluorescents.
  5. Substrat polymère selon l'une des revendications précédentes, caractérisé en ce que le substrat polymère est choisi dans le groupe consistant en les copolymères d'oléfines cycliques (COC), les polymères d'oléfines cycliques (COP), le poly(méthacrylate de méthyle) (PMMA), les polyesters aliphatiques, les polyuréthanes, les polyéthers ou en les composites de ces derniers.
  6. Substrat polymère selon l'une des revendications précédentes, caractérisé en ce que les structures fluorescentes sont constituées de plusieurs éléments sous forme de traits, de lignes, de caractères, de motifs d'interférence ou de combinaisons de ces derniers.
  7. Substrat polymère selon la revendication précédente, caractérisé en ce que les éléments présentent une résolution, suffisante pour le codage d'informations, de 1 µm à 1000 mm, de préférence de 1 µm à 1 mm, d'une manière particulièrement préférée de 1 µm à 100 µm.
  8. Chambre à échantillons contenant un substrat polymère selon l'une des revendications précédentes.
  9. Chambre à échantillons selon la revendication 8, caractérisée en ce que la structure fluorescente et les objets/cellules à récupérer se trouvent dans un plan focal.
  10. Chambre à échantillons selon l'une des revendications 8 ou 9, caractérisée en ce que la grille de récupération est spatialement séparée des objets/cellules à récupérer dans la chambre à échantillons.
  11. Chambre à échantillons selon l'une des revendications 8 à 10, caractérisée en ce que la chambre à échantillons comprend une plaque de fond et une plaque de couverture, un renfoncement étant prévu dans la plaque de fond, de façon à ce qu'il se forme, du fait de la plaque de fond et de la plaque de couverture, une zone de logement, le substrat polymère étant prévu dans la plaque de couverture.
  12. Chambre à échantillons selon la revendication 11, caractérisée en ce que l'évidement aménagé dans la plaque de fond comprend un fond, de façon qu'une cavité soit formée du fait de la plaque de couverture.
  13. Chambre à échantillons selon la revendication 12, caractérisée en ce que la plaque de fond et/ou la plaque de couverture comprennent un canal, en particulier un trou traversant, débouchant de l'extérieur dans la zone de logement.
  14. Chambre à échantillons selon l'une des revendications 11 à 13, caractérisée en ce que la plaque de couverture a une épaisseur de 50 µm - 250 mm, en particulier de 100 µm - 200 µm.
  15. Chambre à échantillons selon l'une des revendications 11 à 14, caractérisée en ce que la plaque de couverture est configurée comme une feuille.
  16. Chambre à échantillons selon la revendication 15, dans laquelle la structure fluorescente est incorporée dans la feuille.
  17. Procédé de fabrication d'un substrat polymère selon l'une des revendications 1 à 7, caractérisé en ce qu'on soumet un substrat polymère au moins par zones à une irradiation dans la plage de longueurs d'onde inférieure à 300 nm, pour produire des structures fluorescentes.
  18. Procédé selon la revendication 17, caractérisé en ce que des structures fluorescentes sont produites à partir de plusieurs éléments sous forme de traits, de lignes, de caractères, de motifs d'interférence ou de combinaisons de ceux-ci.
  19. Procédé selon l'une des revendications 17 ou 18, caractérisé en ce que les éléments sont produits avec une résolution, suffisante pour le codage d'informations, de 1 µm à 1000 mm, de préférence de 1 µm à 1 mm, d'une manière particulièrement préférée de 1 µm à 100 µm.
  20. Procédé selon l'une des revendications 17 à 19, caractérisé en ce que des structures fluorescentes sont produites par irradiation avec des rayons UV, à l'aide de masques par reproduction ou par exposition par contact.
  21. Procédé selon la revendication 20, caractérisé en ce qu'on utilise en tant que masques des plaques portant un motif de domaines transparents et/ou non transparents.
  22. Procédé selon l'une des revendications 17 à 19, caractérisé en ce que des structures fluorescentes sont produites à l'aide de systèmes de positionnement par irradiation avec une lumière cohérente ou focalisée.
  23. Procédé selon l'une des revendications 17 à 22, caractérisé en ce que, pour la production structurée de structures fluorescentes, on utilise des sources de rayonnement ayant des longueurs d'onde d'émission ou des domaines d'émission inférieurs à 300 nm, en particulier choisies dans le groupe consistant en les lampes au deutérium, les lampes à excimère (Xe), les lasers excimères (F2, ArF, KrF) ou les lasers solides (Nd : YVO4/YLF).
  24. Utilisation d'un substrat polymère selon l'une des revendications 1 à 7 dans une chambre à échantillons.
  25. Utilisation du substrat polymère selon l'une des revendications 1 à 7 en tant que marqueur ou étiquette pour l'authentification ou l'individualisation de produits.
  26. Utilisation selon la revendication 25, caractérisée en ce que les caractéristiques de fluorescence contiennent des informations, codées ou non codées, pour l'authentification et/ou pour l'individualisation de produits.
  27. Utilisation du substrat polymère selon l'une des revendications 1 à 7, en particulier sous forme de feuilles, de blisters et de cavités, en tant qu'éléments d'emballage.
  28. Utilisation selon l'une des revendications 24 à 27, pour laquelle les caractéristiques de fluorescence contiennent des informations sous une forme lisible par les ordinateurs, en particulier des codes-barres ou des motifs à points.
EP09011507.2A 2009-09-08 2009-09-08 Substrat polymère doté d'une structure fluorescente, son procédé de fabrication et son utilisation Active EP2292329B1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP09011507.2A EP2292329B1 (fr) 2009-09-08 2009-09-08 Substrat polymère doté d'une structure fluorescente, son procédé de fabrication et son utilisation
US12/876,881 US9597688B2 (en) 2009-09-08 2010-09-07 Polymer substrate with fluorescent structure, method for the production thereof and the use thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP09011507.2A EP2292329B1 (fr) 2009-09-08 2009-09-08 Substrat polymère doté d'une structure fluorescente, son procédé de fabrication et son utilisation

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EP2292329A1 EP2292329A1 (fr) 2011-03-09
EP2292329A9 EP2292329A9 (fr) 2011-05-04
EP2292329B1 true EP2292329B1 (fr) 2014-11-12

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US11977028B2 (en) * 2020-04-15 2024-05-07 Northwestern University Mechanical-bond-induced exciplex fluorescence

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4241663C2 (de) * 1992-12-04 1997-05-28 Borus Spezialverfahren Verfahren zur Kennzeichnung und Erkennung eines Gegenstands
JP3068483B2 (ja) * 1996-02-08 2000-07-24 株式会社東芝 パターン読み出し方法、および光学的信号読み取り装置
FR2755902B1 (fr) * 1996-11-20 1999-02-12 Sarl Ind Laser Partner Procede de realisation, sur un produit, d'un marquage invisible, pouvant etre revele
DE10004135B4 (de) 2000-01-31 2005-04-28 Ibidi Gmbh Kammer für Zellkulturen
NL1015686C2 (nl) * 2000-07-12 2002-01-15 Dsm Nv Werkwijze voor het met laserbestraling irreversibel aanbrengen van een voor het naakte oog onzichtbare markering op een polymeer vormdeel.
US7378280B2 (en) * 2000-11-16 2008-05-27 California Institute Of Technology Apparatus and methods for conducting assays and high throughput screening
DE10105711B4 (de) 2001-02-08 2005-03-10 Ibidi Gmbh Probenträger für chemische und biologische Proben
JP2002239484A (ja) * 2001-02-16 2002-08-27 Ushio Inc 誘電体バリア放電ランプを使った基板処理装置
EP1458483B1 (fr) 2001-09-28 2014-01-08 ibidi GmbH Chambre d'ecoulement
US8206666B2 (en) * 2002-05-21 2012-06-26 Battelle Memorial Institute Reactors having varying cross-section, methods of making same, and methods of conducting reactions with varying local contact time
GB0212638D0 (en) 2002-05-31 2002-07-10 Ucb Sa Authentication means
GB0307615D0 (en) 2003-04-02 2003-05-07 Ucb Sa Authentication means
US7169615B2 (en) 2003-11-26 2007-01-30 General Electric Company Method of authenticating polymers, authenticatable polymers, methods of making authenticatable polymers and authenticatable articles, and articles made there from
JP4945893B2 (ja) * 2004-11-11 2012-06-06 大日本印刷株式会社 パターン形成用基板
DE502005011085D1 (de) * 2005-07-05 2011-04-21 Ibidi Gmbh Mikrofluid-Vorrichtung und Verfahren zur Erzeugung diffusiv aufgebauter Gradienten
DK1880764T3 (da) 2006-07-20 2012-12-17 Ibidi Gmbh Prøveholder til undersøgelse af cellevækst
FR2909922B1 (fr) * 2006-12-14 2010-08-13 Att Advanced Track & Trace Procede et dispositif de marquage d'objets et materiaux.
EP2263797B1 (fr) 2007-06-25 2011-09-07 ibidi GmbH Chambre d'analyse
US20100186524A1 (en) * 2008-02-05 2010-07-29 Enertechnix, Inc Aerosol Collection and Microdroplet Delivery for Analysis

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Publication number Publication date
US9597688B2 (en) 2017-03-21
EP2292329A1 (fr) 2011-03-09
US20110086420A1 (en) 2011-04-14
EP2292329A9 (fr) 2011-05-04

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