EP1470242A4 - Enzymatic cycling assays for homocysteine and cystathionine - Google Patents

Enzymatic cycling assays for homocysteine and cystathionine

Info

Publication number
EP1470242A4
EP1470242A4 EP02789500A EP02789500A EP1470242A4 EP 1470242 A4 EP1470242 A4 EP 1470242A4 EP 02789500 A EP02789500 A EP 02789500A EP 02789500 A EP02789500 A EP 02789500A EP 1470242 A4 EP1470242 A4 EP 1470242A4
Authority
EP
European Patent Office
Prior art keywords
homocysteine
cystathionine
derivative
pyruvate
assay
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP02789500A
Other languages
German (de)
French (fr)
Other versions
EP1470242B1 (en
EP1470242A2 (en
Inventor
Heather Kay Webb
Jeffrey Owens
Raymond Liedtke
Doreen Forest
Mark Legaz
Glenn Kawasaki
Sobomabo Lawson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Axis Shield Diagnostics Ltd
Original Assignee
Catch Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=21756556&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP1470242(A4) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Catch Inc filed Critical Catch Inc
Publication of EP1470242A2 publication Critical patent/EP1470242A2/en
Publication of EP1470242A4 publication Critical patent/EP1470242A4/en
Application granted granted Critical
Publication of EP1470242B1 publication Critical patent/EP1470242B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6806—Determination of free amino acids
    • G01N33/6812—Assays for specific amino acids
    • G01N33/6815—Assays for specific amino acids containing sulfur, e.g. cysteine, cystine, methionine, homocysteine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention provides an enyzmatic cycling assay for assessing the amount of homocysteine and/or cystathionine in a solution such as blood, blood derivatives, or urine. The assay comprises the steps of contacting the solution containing homocysteine and/or cystathionine to form a reaction mixture, with CBS, or derivative thereof, L-serine, and CBL or a derivative thereof for a time period sufficient to catalyze the cyclical conversion of homocysteine to cystathionine and the reconversion of cystathionine to homocysteine with the production of pyruvate and ammonia and determining the amount of homocysteine and/or ammonia present in the reaction mixture. Expression vectors, isolation procedures for CBS, or derivative thereof, L-serine and CBL or derivative thereof and test kits for carrying out the assay are provided. Figure (1) depicts absorbance versus time plots obtained using aqueous solutions of pyruvate (5-100UM) demonstrating the quantitation of pyruvate.
EP02789500.2A 2001-11-06 2002-11-06 Enzymatic cycling assays for homocysteine and cystathionine Expired - Lifetime EP1470242B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US12762 2001-11-06
US10/012,762 US6635438B2 (en) 1999-11-02 2001-11-06 Enzymatic cycling assays for homocysteine and cystathionine
PCT/US2002/035777 WO2003040694A2 (en) 2001-11-06 2002-11-06 Enzymatic cycling assays for homocysteine and cystathionine

Publications (3)

Publication Number Publication Date
EP1470242A2 EP1470242A2 (en) 2004-10-27
EP1470242A4 true EP1470242A4 (en) 2007-01-03
EP1470242B1 EP1470242B1 (en) 2013-08-14

Family

ID=21756556

Family Applications (1)

Application Number Title Priority Date Filing Date
EP02789500.2A Expired - Lifetime EP1470242B1 (en) 2001-11-06 2002-11-06 Enzymatic cycling assays for homocysteine and cystathionine

Country Status (12)

Country Link
US (2) US6635438B2 (en)
EP (1) EP1470242B1 (en)
CN (1) CN1612937B (en)
AU (1) AU2002352538A1 (en)
BR (1) BRPI0213936B8 (en)
DK (1) DK1470242T3 (en)
HU (1) HU228343B1 (en)
NO (1) NO20042144L (en)
RU (1) RU2344176C2 (en)
SK (1) SK288189B6 (en)
WO (1) WO2003040694A2 (en)
ZA (1) ZA200403395B (en)

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US6635438B2 (en) * 1999-11-02 2003-10-21 Catch, Inc. Enzymatic cycling assays for homocysteine and cystathionine
GB0008784D0 (en) * 2000-04-10 2000-05-31 Axis Shield Plc Assay
GB0311804D0 (en) * 2003-05-22 2003-06-25 Univ Glasgow Improved homocysteine assay
US7097968B2 (en) * 2003-07-10 2006-08-29 General Atomics Methods and compositions for assaying homocysteine
US8476034B2 (en) * 2003-07-10 2013-07-02 General Atomics Methods and compositions for assaying homocysteine
DE10342264C5 (en) 2003-09-12 2012-10-31 Leica Biosystems Nussloch Gmbh System for uniquely assigning histological cassettes and slides
GB0428130D0 (en) * 2004-12-22 2005-01-26 Oxford Biosensors Ltd Selective HDL cholesterol assay
CA2618699C (en) 2005-08-11 2012-10-02 J. Craig Venter Institute, Inc. In vitro recombination method
AU2006329215A1 (en) * 2005-12-22 2007-06-28 Conjuchem Biotechnologies Inc. Process for the production of preformed conjugates of albumin and a therapeutic agent
WO2009039298A2 (en) * 2007-09-18 2009-03-26 University Of Florida Research Foundation, Inc. Sensors using aigan/gan high electron mobility transistors
JP2011510297A (en) * 2008-01-18 2011-03-31 バトリックス・メディカル・インコーポレイテッド Diagnostic biomarkers for aneurysms
WO2010054159A2 (en) * 2008-11-06 2010-05-14 University Of Florida Research Foundation, Inc. Materials and methods for detecting toxins, pathogens and other biological materials
RU2012132313A (en) * 2009-12-30 2014-02-10 Зэ Стейт Оф Орегон Эктинг Бай Энд Сру Зэ Стейт Боард Оф Хайе Эдьюкейшн Он Бехалф Оф Портланд Стейт Юниверсити DETECTION OF THIOL
US9216209B1 (en) 2011-06-06 2015-12-22 Kilmer S. McCully Compositions and method for utilization of thioretinamide in therapy of degenerative diseases of aging
RU2471181C1 (en) * 2011-06-20 2012-12-27 Открытое акционерное общество Научно-производственное объединение "Химавтоматика" Method for determination of total antioxidant and anthocyan content in food products and beverages
US9034318B2 (en) * 2011-08-30 2015-05-19 The Regents Of The University Of Colorado, A Body Corporate Chemically modified cystathionine beta-synthase enzyme for treatment of homocystinuria
JP5914019B2 (en) * 2012-02-03 2016-05-11 旭化成ファーマ株式会社 Method for measuring mevalonic acid by enzyme cycling reaction and its calibration sample
WO2014032044A1 (en) 2012-08-24 2014-02-27 Case Western Reserve University System and method for detecting of alpha-methylacyl-coa racemase (amacr) and prostate cancer
CN103674873B (en) * 2013-11-22 2015-05-20 杭州利安生物科技有限公司 Method for quantificationally detecting serine
CN104111338B (en) * 2014-05-07 2016-03-02 山东博科生物产业有限公司 A kind of homocysteine detection kit of strong interference immunity
WO2015183792A1 (en) 2014-05-27 2015-12-03 Case Western Reserve University Electrochemical sensor for analyte detection
US11009479B2 (en) 2014-05-27 2021-05-18 Case Western Reserve University Systems and methods for the detection of HbA1c
EP3149466B1 (en) * 2014-05-27 2019-11-20 Case Western Reserve University System and method for detecting neural injury
CN104198726B (en) * 2014-08-14 2016-07-06 上海睿康生物科技有限公司 A kind of stable homocysteine detection kit
CN105039368A (en) * 2015-07-08 2015-11-11 北京嘉万生物技术有限公司 Recombinant yeast cystathionine beta-synthase catalytic center expression and enlarged production
CN105181970B (en) * 2015-08-28 2017-05-03 宁波瑞源生物科技有限公司 Stable kit for detecting homocysteine
CN106970230B (en) * 2017-03-29 2019-06-11 广州市伊川生物科技有限公司 A kind of homocysteine detection reagent box
CN110346572A (en) * 2018-04-04 2019-10-18 浙江力耐得传动科技有限公司 It is coupled the method and assay kit of pyruvate oxidation enzymatic determination homocysteine (Hcy)
CN108802125B (en) * 2018-08-28 2023-09-15 长沙理工大学 Detection method and sensor of L-cysteine based on hepta (6-mercapto-6-deoxy) -beta-cyclodextrin
CN109975279B (en) * 2019-03-07 2021-05-28 广州悦蜂生物防治科技有限公司 Method, kit and rapid test card for detecting lipase activity
US11697807B2 (en) 2019-09-30 2023-07-11 Case Western Reserve University Electrochemical biosensor
CN110954581A (en) * 2019-12-19 2020-04-03 湖南海源医疗科技股份有限公司 Electrochemical method for rapidly detecting homocysteine
CN114277021B (en) * 2021-11-19 2022-09-20 武汉瀚海新酶生物科技有限公司 Cystathionine-beta-lyase and preparation method and application thereof
CN114807199A (en) * 2022-02-22 2022-07-29 三诺生物传感股份有限公司 Preparation method and application of recombinant cystathionine beta-lyase
CN115725556B (en) * 2022-09-20 2024-05-03 青岛硕景生物科技有限公司 Mutant cystathionine beta lyase with improved stability, and preparation method and application thereof

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US6066467A (en) * 1997-07-24 2000-05-23 Anticancer, Inc. High specificity homocysteine assays for biological samples
US6174696B1 (en) * 1999-11-12 2001-01-16 Genzyme Corporation Method for the determination of homocysteine

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WO2000028071A2 (en) * 1998-11-12 2000-05-18 Genzyme Corporation A method for the determination of homocysteine
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See also references of WO03040694A2 *

Also Published As

Publication number Publication date
AU2002352538A1 (en) 2003-05-19
ZA200403395B (en) 2006-06-28
HUP0500095A2 (en) 2005-05-30
US6635438B2 (en) 2003-10-21
EP1470242B1 (en) 2013-08-14
HU228343B1 (en) 2013-03-28
BR0213936A (en) 2004-08-31
WO2003040694A2 (en) 2003-05-15
BRPI0213936B8 (en) 2021-07-27
WO2003040694A3 (en) 2004-08-19
CN1612937B (en) 2010-05-05
NO20042144L (en) 2004-08-06
US6867014B2 (en) 2005-03-15
HUP0500095A3 (en) 2010-03-29
SK2042004A3 (en) 2004-10-05
US20040096929A1 (en) 2004-05-20
RU2344176C2 (en) 2009-01-20
BRPI0213936B1 (en) 2015-08-25
DK1470242T3 (en) 2013-11-11
RU2004117087A (en) 2005-04-27
EP1470242A2 (en) 2004-10-27
SK288189B6 (en) 2014-05-06
US20030138872A1 (en) 2003-07-24
CN1612937A (en) 2005-05-04

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