EP1336093A1 - Test kit for simultaneously detecting several types of residues - Google Patents
Test kit for simultaneously detecting several types of residuesInfo
- Publication number
- EP1336093A1 EP1336093A1 EP01993825A EP01993825A EP1336093A1 EP 1336093 A1 EP1336093 A1 EP 1336093A1 EP 01993825 A EP01993825 A EP 01993825A EP 01993825 A EP01993825 A EP 01993825A EP 1336093 A1 EP1336093 A1 EP 1336093A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- residues
- residue
- adsorption
- color reaction
- different
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N21/88—Investigating the presence of flaws or contamination
- G01N21/8803—Visual inspection
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N2001/028—Sampling from a surface, swabbing, vaporising
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
Definitions
- the present invention relates to a method for differentiating residues by type on hard surfaces.
- the invention further relates to the use of the method according to the invention for residue control in the food, pharmaceutical or cosmetics industry and in food processing companies.
- the invention also relates to bodies for the simultaneous detection of at least two different types of residues, which are equipped with one or more adsorption materials suitable for absorbing residues.
- Detergents of different compositions are used to remove this contamination.
- the selection of a suitable cleaning agent is usually based on the type of dirt to be removed. Knowing the type of soiling, the cleaning agent can be selected accordingly.
- detergent components make a difference in cleaning performance compared to protein, fat and carbohydrates. After a cleaning cycle, small residues of dirt may remain, which are hardly noticeable visually, but pose a microbiological risk.
- control of hygiene by bacteriological methods can also be falsified by disinfection, which does not rule out the possibility of organic contaminants accumulating, which in the end are the cause of the growth of resistant microorganisms. Furthermore, the selective removal of the samples does not guarantee that entire devices and larger surfaces can be checked for their hygiene standards.
- PCT publication WO 90/14591 describes a method for cleaning and at the same time checking the cleaning success and hygiene by applying a solution of a mixture of a cleaning agent and a dye or a dye combination to surfaces to be cleaned.
- the organic contamination that remains on the surface after cleaning becomes visible through coloring.
- EP-A-0 347494 describes a method for the detection of impurities on an object by treating it with a dye solution and then reacting it with a developer solution. This also makes it possible to localize the contaminants, but not to differentiate them according to their type.
- the coloring of food residues by indicators is known from odontology.
- the indicators for the visualization of dental plaque are used here. Here too it is only possible to state that there are food residues on the teeth.
- the granted European patent EP 715 713 B1 discloses a method which makes it possible, after a cleaning cycle, to determine what type of organic residues are on the surface, in order to be able to use specific cleaners for a possibly necessary subsequent cleaning or to use cleaners suitable for the future or to be able to use combinations of cleaners and thus avoid subsequent cleaning.
- EP 715 713 B1 teaches how the type of organic residues on the surface can be determined even before the actual cleaning, so that a suitable detergent composition can be used immediately.
- EP 715 713 B1 attempts to meet this need by disclosing a method for differentiating organic food residues, in which by applying a solution containing a combination of at least two dyes, at least one of which has a hydrophilic character and at least one has a hydrophobic character , on the hard surface and subsequent visual determination, the type of residue is recognizable by its specific coloring.
- the Ver- Driving should be easy to carry out and allow the impurity to be differentiated according to fat, protein or carbohydrate without the determination of one type of residue being impaired by the determination of the other types of residue.
- the process should also be feasible in such a way that the formation of poorly soluble residues on the hard surface is avoided as far as possible.
- a method for the simultaneous detection of at least two different types of residues on hard surfaces in which a body which is equipped with one or more adsorption materials suitable for absorbing residues is brought into contact with the surface to be examined, wherein a) the adsorbent material (s) contain chemical substances that are able to undergo a visually recognizable color reaction with different residues, which allows a qualitative assignment to a certain type of residue, preferably the chemical substance by chemical reaction or physical interaction on the or the residue carriers are bound, or b) chemical substances are applied to the adsorption material or residues after the residue absorption, which are capable of a visually recognizable color reaction with different residues to enter into, which allows a qualitative assignment to a certain type of residue, the chemical substances being applied to the adsorption material (s) manually or preferably automatically with a simple device.
- organic residues selected from fats, proteins and / or carbohydrates are recorded simultaneously as the types of residues. It is further preferred that the color reaction is accelerated by increasing the temperature.
- the chemical substances which undergo a color reaction are preferably selected from ninhydrin, comassie blue, folin reagent, biuret reagent, a-naphthol, diphenylamine, aniline, iodine, lipase and fluorescent dyes, such as 2 ', 7'-dichlorofluorescein, Erythrosin, Ponceau 4 R, curcumin, ß-carotene, riboflavin and Ceres Blau RR or their mixtures.
- fluorescent dyes such as 2 ', 7'-dichlorofluorescein, Erythrosin, Ponceau 4 R, curcumin, ß-carotene, riboflavin and Ceres Blau RR or their mixtures.
- the adsorbing material or materials present in accordance with the method according to the invention preferably consist of solid polymeric material with absorbency, it being particularly preferred that the polymeric material is selected from wool, silk, polyacrylic, viscose, polyamide, polyacetate, lyocell.
- the body according to the method according to the invention is equipped with one or more similar or different adsorption materials in such a way that at least two separate areas of the adsorption material or materials can be visually distinguished.
- a distinction can be made by spatial separation or by other colors or by perforation at the interface or other conceivable demarcation features.
- the method according to the invention is preferably carried out in such a way that the qualitative recording of at least two types of residues side by side is possible.
- the organic residues are preferably fats, proteins and / or carbohydrates, which can be present side by side or individually.
- the user can, without risking the formation of difficult-to-remove color complexes on the surface to be cleaned,
- the method according to the invention also represents a simple method for the qualitative and possibly quantitative statement of the cleaning performance of cleaning agents in the development of a cleaning agent, in that when testing a test soiling (fat / protein / starch) first with the cleaning agent to be examined and then with the Body to be used according to the method of the invention is brought into contact, the body optionally being further treated according to the previous description. Due to the different discoloration due to the individual dirt components, it can be recognized which component can be detached more or less by the cleaning agent. Then more targeted recipe improvements can be made.
- the method according to the invention also makes it possible to make a suitable choice of cleaning agent by detecting the type of soiling before cleaning. After cleaning, the cleaning success can be checked on the one hand and on the other hand a special cleaner suitable for the dirt component that has not been removed can be selected.
- critical points can be defined according to the HACCP principle (Hazard Analysis Critical Control Points). At these critical points the cleaning process according to the invention is carried out and appropriate measures are taken depending on the result.
- the residues to be determined can be picked up from the hard surface to be examined in a known manner, in particular, however, by dabbing or wiping the surface to be examined.
- erythrosine Cl 45430; E 127) and Ponceau 4 R (Cl 16255; E 124) and curcumin (Cl 75300; E 100), ⁇ -carotene (Cl 40800; E 160a), Riboflavin (E 101) and Ceres Blau RR used.
- test strip prepared according to the invention with three separately recognizable adsorption areas Z, F and E is briefly immersed together with the adsorption areas in water which is mixed with 0.1% alkylbenzenesulfonic acid (ABS) and 10% ethanol (for preservation and better wetting).
- ABS alkylbenzenesulfonic acid
- ethanol for preservation and better wetting
- test strip is then clamped in a device and the adsorption areas are sprayed with different reagents: area E is with a 2% solution of diphenylamine in 10% phosphoric acid, area F is with a 0.1% solution of dichlorofluorescein in 96% ethanol and Z surface is sprayed with a 0.5% 'strength by weight solution of ninhydrin in 96% ethanol. If protein residues are present, a purple color can be observed in area E. In the next step, the area F is irradiated and evaluated using a UV lamp (366 nm). If there are fat residues, they can be recognized as light yellow spots on a yellow-green background.
- a UV lamp 366 nm
- the UV lamp is then switched off and the test strip is heated using a heating device.
- the test strip is heated to 150 ° C for 5 minutes.
- a gray-violet coloration of area Z indicates residues of carbohydrates. If necessary, the qualitatively observed colorations can also be quantified by measuring the intensity of the color.
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Engineering & Computer Science (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE10055557 | 2000-11-09 | ||
DE10055557A DE10055557C2 (en) | 2000-11-09 | 2000-11-09 | Procedure for the simultaneous recording of several types of residues |
PCT/EP2001/012636 WO2002039098A1 (en) | 2000-11-09 | 2001-10-31 | Test kit for simultaneously detecting several types of residues |
Publications (1)
Publication Number | Publication Date |
---|---|
EP1336093A1 true EP1336093A1 (en) | 2003-08-20 |
Family
ID=7662702
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP01993825A Withdrawn EP1336093A1 (en) | 2000-11-09 | 2001-10-31 | Test kit for simultaneously detecting several types of residues |
Country Status (5)
Country | Link |
---|---|
US (1) | US20050037501A1 (en) |
EP (1) | EP1336093A1 (en) |
DE (1) | DE10055557C2 (en) |
PL (1) | PL361592A1 (en) |
WO (1) | WO2002039098A1 (en) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007054238A1 (en) * | 2005-11-08 | 2007-05-18 | Unilever N.V. | Hygiene indicator device |
WO2011102936A1 (en) | 2010-02-16 | 2011-08-25 | Ferro Corporation | MATERIALS FOR IMPROVED ADHESION RELATING TO FUNCTIONAL COLD END COATINGS (CECs) AND METHODS OF DETECTING SAME |
SE1250056A1 (en) * | 2012-01-27 | 2013-07-28 | Spaarab Produkter Ab | Detection of contaminated areas |
US9372142B2 (en) * | 2012-02-27 | 2016-06-21 | Wisconsin Alumni Research Foundation | Liquid crystal device and method for screening protein stabilizing agents or optimal protein concentrations to prevent unfolding |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4160008A (en) * | 1978-01-26 | 1979-07-03 | Miles Laboratories, Inc. | Multilayered test device for determining the presence of a liquid sample component, and method of use |
US4301115A (en) * | 1979-06-22 | 1981-11-17 | Miles Laboratories, Inc. | Test device resistant to cross contamination between reactant areas and process for making it |
SE8206250L (en) * | 1982-11-03 | 1984-05-04 | Chemical Dynamics Sweden Ab | REGULATION OF GAS MICROFLORA |
US4532107A (en) * | 1984-03-26 | 1985-07-30 | Miles Laboratories, Inc. | Reagent test device |
US4745797A (en) * | 1984-05-07 | 1988-05-24 | Lockheed Corporation | Method and apparatus for detecting hydrocarbon fuel leaks |
DE3530993A1 (en) * | 1985-08-30 | 1987-03-05 | Miles Lab | TEST STRIP WITH DETERMINABLE SAMPLE CAPACITY |
US4858465A (en) * | 1988-06-21 | 1989-08-22 | Rockwell International Corporation | Water washable contaminant detection and labeling compositions and method for utilizing same |
US5330917A (en) * | 1989-02-02 | 1994-07-19 | Hybrivet Systems, Inc. | Test swab device and method of detecting lead, mercury, arsenic, and bismuth |
US5550061A (en) * | 1989-02-02 | 1996-08-27 | Hybrivet Systems, Inc. | Test swab and method of using same |
DK239589D0 (en) * | 1989-05-17 | 1989-05-17 | Dst Kemi A S | PROCEDURE FOR SIMULTAN CLEANING AND HYGIENE CONTROL AND METHOD FOR USING THE PROCEDURE |
CA2116061C (en) * | 1993-03-02 | 1998-12-22 | George H. Geisinger | Unit test, kit, and method for qualitative identification of a suspect substance |
WO1995006241A1 (en) * | 1993-08-23 | 1995-03-02 | Henkel Kommanditgesellschaft Auf Aktien | Process for differentiating organic foodstuff residues on hard surfaces |
US5726062A (en) * | 1995-04-19 | 1998-03-10 | Konica Corporation | Method of detecting protein and a kit detecting protein using the same |
AU5338399A (en) * | 1998-08-14 | 2000-03-06 | Biocontrol Systems, Incorporated | Detection of contaminants using self-contained devices employing target materialbinding dyes |
DE60015832T2 (en) * | 1999-03-24 | 2005-10-27 | JohnsonDiversey, Inc., Sturtevant | Method of detecting protein and kit therefor |
-
2000
- 2000-11-09 DE DE10055557A patent/DE10055557C2/en not_active Expired - Fee Related
-
2001
- 2001-10-31 PL PL36159201A patent/PL361592A1/en unknown
- 2001-10-31 EP EP01993825A patent/EP1336093A1/en not_active Withdrawn
- 2001-10-31 US US10/416,355 patent/US20050037501A1/en not_active Abandoned
- 2001-10-31 WO PCT/EP2001/012636 patent/WO2002039098A1/en not_active Application Discontinuation
Non-Patent Citations (1)
Title |
---|
See references of WO0239098A1 * |
Also Published As
Publication number | Publication date |
---|---|
DE10055557C2 (en) | 2002-09-26 |
WO2002039098A1 (en) | 2002-05-16 |
US20050037501A1 (en) | 2005-02-17 |
DE10055557A1 (en) | 2002-05-23 |
PL361592A1 (en) | 2004-10-04 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 20030430 |
|
AK | Designated contracting states |
Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR |
|
AX | Request for extension of the european patent |
Extension state: AL LT LV MK RO SI |
|
RIN1 | Information on inventor provided before grant (corrected) |
Inventor name: MEYER, BERNHARD Inventor name: TYBORSKI, THOMAS |
|
RAP1 | Party data changed (applicant data changed or rights of an application transferred) |
Owner name: ECOLAB INC. |
|
17Q | First examination report despatched |
Effective date: 20061011 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 20070222 |