EP1259537A4 - Systeme d'expression genique a base de recepteurs chimeres - Google Patents

Systeme d'expression genique a base de recepteurs chimeres

Info

Publication number
EP1259537A4
EP1259537A4 EP01912956A EP01912956A EP1259537A4 EP 1259537 A4 EP1259537 A4 EP 1259537A4 EP 01912956 A EP01912956 A EP 01912956A EP 01912956 A EP01912956 A EP 01912956A EP 1259537 A4 EP1259537 A4 EP 1259537A4
Authority
EP
European Patent Office
Prior art keywords
gene expression
system based
expression system
chimeric receptors
chimeric
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP01912956A
Other languages
German (de)
English (en)
Other versions
EP1259537A1 (fr
Inventor
Fred H Gage
Steven T Suhr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Salk Institute for Biological Studies
Original Assignee
Salk Institute for Biological Studies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Salk Institute for Biological Studies filed Critical Salk Institute for Biological Studies
Publication of EP1259537A1 publication Critical patent/EP1259537A1/fr
Publication of EP1259537A4 publication Critical patent/EP1259537A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
EP01912956A 2000-02-24 2001-02-23 Systeme d'expression genique a base de recepteurs chimeres Withdrawn EP1259537A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18459100P 2000-02-24 2000-02-24
US184591P 2000-02-24
PCT/US2001/005750 WO2001062780A1 (fr) 2000-02-24 2001-02-23 Systeme d'expression genique a base de recepteurs chimeres

Publications (2)

Publication Number Publication Date
EP1259537A1 EP1259537A1 (fr) 2002-11-27
EP1259537A4 true EP1259537A4 (fr) 2004-05-12

Family

ID=22677535

Family Applications (1)

Application Number Title Priority Date Filing Date
EP01912956A Withdrawn EP1259537A4 (fr) 2000-02-24 2001-02-23 Systeme d'expression genique a base de recepteurs chimeres

Country Status (3)

Country Link
EP (1) EP1259537A4 (fr)
AU (1) AU2001241682A1 (fr)
WO (1) WO2001062780A1 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8105825B2 (en) 2000-10-03 2012-01-31 Intrexon Corporation Multiple inducible gene regulation system
MXPA03007492A (es) 2001-02-20 2004-10-15 Rheogene Holdings Inc Receptores de retinoide x quimerico y su uso en un sistema de expresion de gen inducible basado en receptor de ecdisona novedoso.
WO2002066615A2 (fr) 2001-02-20 2002-08-29 Rheogene, Inc. Nouveaux recepteurs a mutant de substitution et utilisation de ceux-ci dans un systeme d'expression de gene inductible fonde sur un recepteur nucleaire
EP1373470B1 (fr) 2001-02-20 2013-04-24 Intrexon Corporation Nouveaux recepteurs de mutants de substitution et utilisation de ceux-ci dans un systeme d'expression genique inductible a base de recepteurs nucleaires
EP1456346B1 (fr) * 2001-02-20 2012-02-15 Intrexon Corporation Nouveau systeme d'expression genetique inductible base sur le recepteur d'ecdysone/recepteur de retinoide x invertebre
US7563879B2 (en) 2001-09-26 2009-07-21 Intrexon Corporation Leafhopper ecdysone receptor nucleic acids, polypeptides, and uses thereof
US7304161B2 (en) 2003-02-10 2007-12-04 Intrexon Corporation Diaclhydrazine ligands for modulating the expression of exogenous genes in mammalian systems via an ecdysone receptor complex
US7456315B2 (en) 2003-02-28 2008-11-25 Intrexon Corporation Bioavailable diacylhydrazine ligands for modulating the expression of exogenous genes via an ecdysone receptor complex
US7935510B2 (en) 2004-04-30 2011-05-03 Intrexon Corporation Mutant receptors and their use in a nuclear receptor-based inducible gene expression system
WO2014144380A1 (fr) 2013-03-15 2014-09-18 Intrexon Corporation Diacylhydrazines contenant du bore
SG11201701944WA (en) 2014-09-17 2017-04-27 Intrexon Corp Boron-containing diacylhydrazine compounds
AU2019207409B2 (en) 2018-01-12 2023-02-23 Basf Se Gene underlying the number of spikelets per spike qtl in wheat on chromosome 7a
WO2019224359A1 (fr) 2018-05-25 2019-11-28 Basf Se Plantes comprenant des gènes de restauration de la stérilité mâle cytoplasmique de type g du blé, et leurs utilisations
BR112020023773A2 (pt) 2018-05-25 2021-03-23 Basf Se molécula de ácido nucleico, polipeptídeo, gene quimérico, células vegetais e métodos para produzir uma célula vegetal, para converter uma planta, para selecionar uma planta, para restaurar a fertilidade, para identificar e/ou selecionar uma planta, para produzir uma planta, para produzir sementes, para aumentar a expressão de um polipeptídeo, usos de ácidos nucleicos e de uma planta
CA3099621A1 (fr) 2018-05-25 2019-11-28 Basf Se Plantes comprenant des genes de restauration de la sterilite male cytoplasmique de type g du ble, et leurs utilisations
WO2019234231A1 (fr) 2018-06-08 2019-12-12 Basf Se Plantes comprenant des gènes de restauration de la stérilité mâle cytoplasmique de type g du blé et leurs utilisations

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993011235A1 (fr) * 1991-12-06 1993-06-10 The Salk Institute For Biological Studies Formes multimeres des membres de la superfamille des recepteurs steroides/thyroides
WO1997038117A1 (fr) * 1996-04-05 1997-10-16 The Salk Institute For Biological Studies Techniques liees a une utilisation d'hormones visant a moduler l'expression de genes exogenes chez des mammiferes et produits connexes
US5830462A (en) * 1993-02-12 1998-11-03 President & Fellows Of Harvard College Regulated transcription of targeted genes and other biological events
WO1999002683A1 (fr) * 1997-07-10 1999-01-21 The Salk Institute For Biological Studies Proteines multifonctionnelles hybrides et recepteurs de lepidopterane modifies destines a etre utilises pour reguler l'expression transgenique
WO1999010508A1 (fr) * 1997-08-27 1999-03-04 Ariad Gene Therapeutics, Inc. Activateurs transcriptionnels chimeres, compositions et applications afferentes
US5880333A (en) * 1995-03-03 1999-03-09 Novartis Finance Corporation Control of gene expression in plants by receptor mediated transactivation in the presence of a chemical ligand
EP0965644A2 (fr) * 1998-06-17 1999-12-22 Rohm And Haas Company Ligands qui module l'expression des genes exogenes à travers du complexe de recepteur d'ecdysone
WO2001036447A2 (fr) * 1999-10-20 2001-05-25 The Salk Institute For Biological Studies Entites fonctionnelles de recepteur hormonal et procedes d'utilisation
WO2002066612A2 (fr) * 2001-02-20 2002-08-29 Rheogene, Inc. Nouveaux recepteurs de mutants de substitution et utilisation de ceux-ci dans un systeme d'expression genique inductible a base de recepteurs nucleaires

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4981784A (en) * 1987-12-02 1991-01-01 The Salk Institute For Biological Studies Retinoic acid receptor method
US5834213A (en) * 1991-05-02 1998-11-10 Baylor College Of Medicine Screening system and assay for identifying compounds that regulate steroid and orphan receptors mediation of DNA transcription
US5728548A (en) * 1995-06-29 1998-03-17 Genetics Institute, Inc. Retinoid receptor-1 (RR1) and DNA encoding RR1

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993011235A1 (fr) * 1991-12-06 1993-06-10 The Salk Institute For Biological Studies Formes multimeres des membres de la superfamille des recepteurs steroides/thyroides
US5830462A (en) * 1993-02-12 1998-11-03 President & Fellows Of Harvard College Regulated transcription of targeted genes and other biological events
US5880333A (en) * 1995-03-03 1999-03-09 Novartis Finance Corporation Control of gene expression in plants by receptor mediated transactivation in the presence of a chemical ligand
WO1997038117A1 (fr) * 1996-04-05 1997-10-16 The Salk Institute For Biological Studies Techniques liees a une utilisation d'hormones visant a moduler l'expression de genes exogenes chez des mammiferes et produits connexes
WO1999002683A1 (fr) * 1997-07-10 1999-01-21 The Salk Institute For Biological Studies Proteines multifonctionnelles hybrides et recepteurs de lepidopterane modifies destines a etre utilises pour reguler l'expression transgenique
WO1999010508A1 (fr) * 1997-08-27 1999-03-04 Ariad Gene Therapeutics, Inc. Activateurs transcriptionnels chimeres, compositions et applications afferentes
EP0965644A2 (fr) * 1998-06-17 1999-12-22 Rohm And Haas Company Ligands qui module l'expression des genes exogenes à travers du complexe de recepteur d'ecdysone
WO2001036447A2 (fr) * 1999-10-20 2001-05-25 The Salk Institute For Biological Studies Entites fonctionnelles de recepteur hormonal et procedes d'utilisation
WO2002066612A2 (fr) * 2001-02-20 2002-08-29 Rheogene, Inc. Nouveaux recepteurs de mutants de substitution et utilisation de ceux-ci dans un systeme d'expression genique inductible a base de recepteurs nucleaires

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LE DOUARIN B ET AL: "A NEW VERSION OF THE TWO-HYBRID ASSAY FOR DETECTION OF PROTEIN-PROTEIN INTERACTIONS", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 23, no. 5, 11 March 1995 (1995-03-11), pages 876 - 878, XP002012441, ISSN: 0305-1048 *
See also references of WO0162780A1 *

Also Published As

Publication number Publication date
WO2001062780A1 (fr) 2001-08-30
AU2001241682A1 (en) 2001-09-03
EP1259537A1 (fr) 2002-11-27

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