EP1246843A1 - Neues fgf homolog zfgf12 - Google Patents

Neues fgf homolog zfgf12

Info

Publication number: EP1246843A1
Authority: EP; European Patent Office
Prior art keywords: residue; zfgf12; polypeptide; cells; sequence
Prior art date: 2000-01-05
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Withdrawn

Application number

EP01901722A

Other languages

English (en)

French (fr)

Inventor

Darrell C. Conklin

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Zymogenetics Inc

Original Assignee

Zymogenetics Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-01-05

Filing date

2001-01-04

Publication date

2002-10-09

2001-01-04 Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc

2002-10-09 Publication of EP1246843A1 publication Critical patent/EP1246843A1/de

Status Withdrawn legal-status Critical Current

Links

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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/12—Antihypertensives
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2799/00—Uses of viruses
- C12N2799/02—Uses of viruses as vector
- C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
- C12N2799/022—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from an adenovirus

Definitions

a “protein” is a macromolecule comprising one or more polypeptide chains.
a protein may also comprise non-peptidic components, such as carbohydrate groups. Carbohydrates and other non-peptidic substituents may be added to a protein by the cell in which the protein is produced, and will vary with the type of cell. Proteins are defined herein in terms of their amino acid backbone structures; substituents such as carbohydrate groups are generally not specified, but may be present nonetheless.
the term "receptor” denotes a cell-associated protein that binds to a bioactive molecule (i.e., a ligand) and mediates the effect of the ligand on the cell.
Membrane-bound receptors are characterized by a multi-peptide structure comprising an extracellular ligand-binding domain and an intracellular effector domain that is typically involved in signal transduction. Binding of ligand to receptor results in a conformational change in the receptor that causes an interaction between the effector domain and other molecule(s) in the cell. This interaction in turn leads to an alteration in the metabolism of the cell. Metabolic events that are linked to receptor-ligand interactions include gene transcription, phosphorylation, dephosphorylation, increases in cyclic AMP production, mobilization of cellular calcium, mobilization of membrane lipids, cell adhesion, hydrolysis of inositol lipids and hydrolysis of phospholipids.
the above conditions are meant to serve as a guide and it is well within the abilities of one skilled in the art to adapt these conditions for use with a particular polypeptide hybrid.
the T m for a specific target sequence is the temperature (under defined conditions) at which 50% of the target sequence will hybridize to a perfectly matched probe sequence.
Those conditions which influence the T ⁇ include, the size and base pair content of the polynucleotide probe, the ionic strength of the hybridization solution, and the presence of destabilizing agents in the hybridization solution.
Insect cells can be infected with recombinant baculovirus, commonly derived from Autographa californica nuclear polyhedrosis virus (AcNPV).
AcNPV Autographa californica nuclear polyhedrosis virus
a preferred amplifiable selectable marker is dihydrofolate reductase, which confers resistance to methotrexate.
Other drug resistance genes e.g. hygromycin resistance, multi-drug resistance, puromycin acetyltransferase
hygromycin resistance e.g. hygromycin resistance, multi-drug resistance, puromycin acetyltransferase
Exemplary chromatographic media include those media derivatized with phenyl, butyl, or octyl groups, such as Phenyl-Sepharose FF (Pharmacia), Toyopearl butyl 650 (Toso Haas, Montgomeryville, PA), Octyl-Sepharose (Pharmacia) and the like; or polyacrylic resins, such as Amberchrom CG 71 (Toso Haas) and the like.
Suitable solid supports include glass beads, silica-based resins, cellulosic resins, agarose beads, cross-linked agarose beads, polystyrene beads, cross- linked polyacrylamide resins and the like that are insoluble under the conditions in which they are to be used.
Protein synthesis may be evaluated, for example, by comparing precipitation of 35 S-methionine-labeled proteins following incubation of the test cells with s S-methionine and 35 S-methionine and a putative modulator of protein synthesis.
Thermogenesis may be evaluated as described by B. Stanley in The Biology of Neuropeptide Y and Related Peptides, W. Colmers and C. Wahlestedt (eds.),
assays known to those skilled in the art can be utilized to detect antibodies which specifically bind to zFGF12 proteins or peptides. Exemplary assays are described in detail in Antibodies: A Laboratory Manual, Harlow and Lane (Eds.), Cold Spring Harbor Laboratory Press, 1988. Representative examples of such assays include: concurrent immunoelectrophoresis, radioimmunoassay, radioimmuno- precipitation, enzyme-linked immunosorbent assay (ELISA), dot blot or Western blot assay, inhibition or competition assay, and sandwich assay. In addition, antibodies can be screened for binding to wild-type versus mutant zFGF12 protein or peptide.
Nucleotide sequences encoding the polypeptides can be obtained in a number of ways, such as through random mutagenesis and random polynucleotide synthesis. These random peptide display libraries can be used to screen for peptides which interact with a known target which can be a protein or polypeptide, such as a ligand or receptor, a biological or synthetic macromolecule, or organic or inorganic substances.
a known target can be a protein or polypeptide, such as a ligand or receptor, a biological or synthetic macromolecule, or organic or inorganic substances.
ischemia and reperfusion are important contributors to tissue necrosis, such as a myocardial infarct or stroke.
the molecules of the present invention will have therapeutic value to reduce damage to the tissues caused by ischemia or ischemia-reperfusion events, particularly in the heart or brain.
Other therapeutic uses for the present invention include induction of skeletal muscle neogenesis and/or hype ⁇ lasia, kidney regeneration and/or for treatment of systemic and pulmonary hypertension.
the white, virus/PEG precipitate from 2 bottles is resuspended in 2.5 ml PBS.
the resulting virus solution is placed in 2-ml microcentrifuge tubes and centrifuged at 14,000 X G in the microcentrifuge for 10 minutes to remove any additional cell debris.
the supernatant from the 2-ml microcentrifuge tubes is transferred into a 15-ml polypropylene snapcap tube and adjusted to a density of 1.34 g/ml with CsCl.
the solution is transferred to 3.2-ml, polycarbonate, thick-walled centrifuge tubes and spun at 348,000 X G for 3-4 hours at 25?C.
the virus forms a white band. Using wide-bore pipette tips, the virus band is collected.

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Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Medicinal Chemistry (AREA)
General Health & Medical Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
General Chemical & Material Sciences (AREA)
Public Health (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Veterinary Medicine (AREA)
Pharmacology & Pharmacy (AREA)
Bioinformatics & Cheminformatics (AREA)
Animal Behavior & Ethology (AREA)
Engineering & Computer Science (AREA)
Urology & Nephrology (AREA)
Cardiology (AREA)
Diabetes (AREA)
Heart & Thoracic Surgery (AREA)
Neurology (AREA)
Toxicology (AREA)
Biochemistry (AREA)
Hematology (AREA)
Vascular Medicine (AREA)
Biomedical Technology (AREA)
Endocrinology (AREA)
Neurosurgery (AREA)
Ophthalmology & Optometry (AREA)
Dermatology (AREA)
Pulmonology (AREA)
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Zoology (AREA)
Gastroenterology & Hepatology (AREA)
Obesity (AREA)
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Genetics & Genomics (AREA)
Molecular Biology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Orthopedic Medicine & Surgery (AREA)
Physical Education & Sports Medicine (AREA)

EP01901722A 2000-01-05 2001-01-04 Neues fgf homolog zfgf12 Withdrawn EP1246843A1 (de)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US47806200A	2000-01-05	2000-01-05
US478062		2000-01-05
PCT/US2001/000238 WO2001049740A1 (en)	2000-01-05	2001-01-04	Novel fgf homolog zfgf12

Publications (1)

Publication Number	Publication Date
EP1246843A1 true EP1246843A1 (de)	2002-10-09

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ID=23898370

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP01901722A Withdrawn EP1246843A1 (de)	2000-01-05	2001-01-04	Neues fgf homolog zfgf12

Country Status (5)

Country	Link
EP (1)	EP1246843A1 (de)
JP (1)	JP2003518944A (de)
AU (1)	AU2759201A (de)
CA (1)	CA2396401A1 (de)
WO (1)	WO2001049740A1 (de)

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EP1947182A1 (de) *	2000-02-15	2008-07-23	Amgen Inc.	Fibroblastenwachstumsfaktor-23-Moleküle und ihre Verwendung
AU2001245535A1 (en) *	2000-03-08	2001-09-17	Chiron Corporation	Human fgf-23 gene and gene expression products
AU2001249125A1 (en) *	2000-03-08	2001-09-17	Chiron Corporation	Human fgf-23 gene and gene expression products
JP2004504063A (ja)	2000-07-19	2004-02-12	アドバンスド　リサーチ　＆　テクノロジー　インスティテュート	新規線維芽細胞増殖因子（ｆｇｆ２３）および使用方法
CN101275136A (zh) *	2000-08-11	2008-10-01	麒麟医药株式会社	调节磷酸代谢,钙代谢,钙化和维生素d代谢的多肽及其编码dna
ATE441666T1 (de) *	2001-12-28	2009-09-15	Kyowa Hakko Kirin Co Ltd	Antikírper gegen den fibroblastenwachstumsfaktor 23
EP1728514A1 (de) *	2005-06-03	2006-12-06	Immunotech S.A.	Verwendungen von Oligonukleotiden welche mesenchymale Stammzellen Proliferation verursachen
US7883705B2 (en)	2007-02-14	2011-02-08	Kyowa Hakko Kirin Co., Ltd.	Anti FGF23 antibody and a pharmaceutical composition comprising the same
PT2726511T (pt)	2011-07-01	2019-10-14	Ngm Biopharmaceuticals Inc	Composições, usos e métodos para o tratamento de distúrbios e doenças metabólicas
US9290557B2 (en)	2012-11-28	2016-03-22	Ngm Biopharmaceuticals, Inc.	Compositions comprising variants and fusions of FGF19 polypeptides
EP3798228A1 (de)	2012-11-28	2021-03-31	NGM Biopharmaceuticals, Inc.	Zusammensetzungen und verfahren zur behandlung von stoffwechselstörungen und -erkrankungen
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KR102489475B1 (ko)	2014-01-24	2023-01-20	엔지엠 바이오파마슈티컬스, 아이엔씨.	결합 단백질 및 그의 사용 방법
WO2015183890A2 (en)	2014-05-28	2015-12-03	Ngm Biopharmaceuticals, Inc.	Methods and compositions for the treatment of metabolic disorders and diseases
US10456449B2 (en)	2014-06-16	2019-10-29	Ngm Biopharmaceuticals, Inc.	Methods and uses for modulating bile acid homeostasis and treatment of bile acid disorders and diseases
UA123763C2 (uk)	2014-10-23	2021-06-02	Енджіем Байофармасьютикалз, Інк.	Фармацевтична композиція для контролю або лікування захворювання або порушення, пов’язаного з fgf19
WO2016073855A1 (en)	2014-11-07	2016-05-12	Ngm Biopharmaceuticals, Inc.	Methods for treatment of bile acid-related disorders and prediction of clinical sensitivity to treatment of bile acid-related disorders
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2001
- 2001-01-04 WO PCT/US2001/000238 patent/WO2001049740A1/en active Application Filing
- 2001-01-04 AU AU27592/01A patent/AU2759201A/en not_active Abandoned
- 2001-01-04 JP JP2001550280A patent/JP2003518944A/ja active Pending
- 2001-01-04 EP EP01901722A patent/EP1246843A1/de not_active Withdrawn
- 2001-01-04 CA CA002396401A patent/CA2396401A1/en not_active Abandoned

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Publication number	Publication date
AU2759201A (en)	2001-07-16
JP2003518944A (ja)	2003-06-17
WO2001049740A1 (en)	2001-07-12
CA2396401A1 (en)	2001-07-12

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Publication	Publication Date	Title
US20080124759A1 (en)	2008-05-29	Novel fgf homolog zfgf12
US20020081663A1 (en)	2002-06-27	Novel FGF homolog ZFGF11
WO2001049849A1 (en)	2001-07-12	Novel fgf homolog zfgf11
EP1246843A1 (de)	2002-10-09	Neues fgf homolog zfgf12
KR100611063B1 (ko)	2006-08-09	섬유아세포 성장인자 상동체
US7563438B2 (en)	2009-07-21	FGF homologs and antibodies thereto
US20050240014A1 (en)	2005-10-27	Connective tissue growth factor homologs
WO1999055869A1 (en)	1999-11-04	Novel polypeptide growth factors and materials and methods for making them
WO1999032515A2 (en)	1999-07-01	Angiopoietin homolog, dna encoding it, and method of making it
US7135317B2 (en)	2006-11-14	Polynucleotides encoding disintegrin homologs, and related products
US20020031805A1 (en)	2002-03-14	Novel FGF homolog zFGF10
US6531576B1 (en)	2003-03-11	Four-helical bundle protein zsig81
WO1999040193A1 (en)	1999-08-12	Angiopoietin homolog zapo3, dna encoding it, and method of making it
WO2001047957A2 (en)	2001-07-05	Human fgf-10- (kgf-2)-like protein zfgf-10
EP1204681A2 (de)	2002-05-15	Schraubenförmiges zytokin zalpha48
WO1999042583A1 (en)	1999-08-26	Connective tissue growth factor homologs
EP1181369B1 (de)	2008-02-13	Vier-helix bündelprotein zsig81
EP1268804A2 (de)	2003-01-02	Helix protein zalpha51
WO2001018205A1 (en)	2001-03-15	Secreted polypeptide zalpha30
AU4197499A (en)	2000-01-05	Immunomodulator polypeptide, zsig57
MXPA00008094A (en)	2001-07-31	Connective tissue growth factor homologs
CA2377580A1 (en)	2001-01-04	Helical polypeptide zalpha29