Classifications

• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
  • A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
  • A01N31/14—Ethers
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
  • A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
  • A01N1/02—Preservation of living parts
  • A01N1/0205—Chemical aspects
  • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
  • A01N1/0221—Freeze-process protecting agents, i.e. substances protecting cells from effects of the physical process, e.g. cryoprotectants, osmolarity regulators like oncotic agents
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
  • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
  • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
• • A—HUMAN NECESSITIES
  • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
  • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
  • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K9/00—Medicinal preparations characterised by special physical form
  • A61K9/08—Solutions

Definitions

• the present invention is generally directed to freeze-resistant topical germicides for application to skin, particularly the teat of a dairy cow, wherein the germicide is a one-part disinfecting composition containing an organic acid germicide and an non-esterifying antifreeze, or a two-part system comprising a first part and a second part adapted to be mixed to yield a disinfecting composition.
• the germicide is a one-part disinfecting composition containing an organic acid germicide and an non-esterifying antifreeze, or a two-part system comprising a first part and a second part adapted to be mixed to yield a disinfecting composition.
• dairymen Since bacterial transfer and proliferation tend to be lower in colder environments, dairymen must balance the potential problems associated with freezing and chapping with the potential for elevated somatic cell counts and clinical mastitis, often choosing to forgo the use of post-milking dips in freezing weather conditions. Other dairymen elect to switch to dips with higher levels of skin softeners and emollients as the temperatures drop, while still others elect dry powder dips, which are basically moisture absorbers with little antimicrobial effectiveness. In an attempt to avoid the above problems, freeze-resistant teat dips have been proposed. For example, a teat-dip composition which freezes below -20°C is disclosed in Japanese Patent No. 8175989.
• the base composition of the dip as provided in one example of that document, comprises about 30% each of propylene glycol and lactic acid, and 7% sorbitan.
• a freeze-resistant teat dip containing chlorhexidine disinfectant, and at least 80 wt% of a volatile alcohol, is disclosed in U.S. Patent No. 4,434,181.
• a non-freezing teat dip which contains, as its base, over 60 wt% of propylene glycol.
• the active germicide in the product is a combination of C8 and CIO alkanoic acids.
• the shelf-life of this product is limited, however, due to tendency of the acid to react with the glycol to form esters, which continuously reduces the amount of available acid in the formulation and thus the product's effectiveness. For example, periodic analysis of such a teat dip indicates a loss of 20% of the acid in only a six-month period at ambient conditions, and over a 10% loss of the acid in just one-month at 100°F.
• a similar problem is encountered if one were to introduce glycol-types of antifreeze into other acid-containing germicidal teat dips.
• the present invention is directed to freeze-resistant topical germicides for application to, for example, the teat of a dairy cow.
• Such compositions resist freezing in ambient winter conditions, and do not react with organic acidic germicides and/or buffering agents. Further, the compositions of this invention maintain compatibility between the freeze-resistant and antimicrobial agents.
• the topical germicides of this invention may generally be classified as one-part or two-part formulations.
• the one-part formulation comprises an organic acid germicide and an non-esterifying antifreeze, while the two-part formulation (hereinafter referred to as a "system") comprises a first part and a second part adapted to be mixed to yield the topical germicide.
• the first part comprises a metal chlorite and a chlorite-stable antifreeze
• the second part comprises (a) an organic acid germicide and a non-esterifying antifreeze, or (b) an inorganic acid and either an alcohol or a non-esterifying antifreeze.
• a one-part freeze- resistant aqueous disinfecting composition containing an organic acid germicide and an non-esterifying antifreeze, wherein the non-esterifying antifreeze contains from 4 to 16 carbon atoms and has no primary carbon atom bearing a hydroxyl group.
• Non-esterifying antifreezes my be an ether having at least one ether linkage between two carbon atoms, an ether-alcohol having at least one ether linkage between two carbon atoms and with at least one secondary carbon atom bearing a hydroxyl group, or an ester having at least one ester linkage between two carbon atoms.
• the non-esterifying antifreeze has the structure R'-O-CH 2 -CH(OR 2 )-R 3 , wherein R 1 is C,. 8 alkyl, and R 2 and R 3 are the same or different and independently selected from hydrogen or C,. g alkyl, and each secondary carbon atom of the C,. 8 alkyl moiety is optionally substituted with a hydroxyl group.
• Representative antifreezes include those compounds wherein R 1 is C,. 8 alkyl moiety, R 2 is hydrogen, and R 3 is C,. 8 alkyl moiety; wherein R 1 is C, .8 alkyl moiety, R 2 is hydrogen and R 3 is methyl; and wherein R 1 , R 2 and R 3 are C, .g alkyl.
• the non-esterifying antifreeze has the structure R 4 -CH(OR 5 )-CH 2 -O-CH 2 -CH(OR 2 )-R 3 , wherein R 2 , R 3 , R 4 and R 5 are the same or different and independently selected from hydrogen and C,. 8 alkyl, and each secondary carbon atom of the C,. 8 alkyl moiety is optionally substituted with a hydroxyl group.
• Representative antifreezes include those compounds wherein R 4 is C,. 8 alkyl and R 5 is hydrogen; and wherein R 4 is C,. 8 alkyl and R 5 is C,. 8 alkyl.
• Non-esterifying antifreezes also include compounds of the above structures wherein at least one oxygen atom is bound to -CO(C j . 8 alkyl) to form an ester.
• Typical non-esterifying antifreezes of this invention include propylene glycol monomethyl ether having the structure CH 3 OCH 2 CH(OH)CH 3 , propylene glycol monoethyl ether having the structure CH 3 CH 2 OCH 2 CH(OH)CH 3 , propylene glycol monopropyl ether having the structure CH 3 CH 2 CH 2 OCH 2 CH(OH)CH 3 , propylene glycol monoisopropyl ether having the structure CH 3 CH(CH 3 )OCH 2 CH(OH)CH 3 , dipropylene glycol having the structure CH 3 CH(OH)CH 2 OCH 2 CH(OH)CH 3 , dipropylene glycol methyl ether having the structure
• the non-esterifying antifreeze may be present at a concentration ranging from about 10% to about 75% by weight of the composition, and typically from 15% to 50% by weight of the composition.
• the organic acid germicide may be an alpha-hydroxy carboxylic acid having a pKa between about 2.8 and about 4.2, such as glycolic acid, lactic acid, malic acid, mandelic acid, citric acid, tartaric acid, and mixtures thereof.
• Other organic acid germicides include formic acid, acetic acid, propionic acid, benzoic acid, caprylic acid, capric acid, hydroxybenzoic acid, and mixtures thereof.
• the organic acid germicide is present at a concentration between about 0.25% and about 7.5% by weight of the disinfecting composition, and typically from 2% and 5% by weight of the disinfecting composition.
• the disinfecting composition may be formulated as solution, cream or gel, and may include one or more optional components such as a textural modifier, a surfactant, an odorant, a colorant, and mixtures thereof.
• a two-part freeze-resistant disinfecting system comprising a first part and a second part adapted to be mixed to yield an aqueous disinfecting composition.
• the first part prior to mixing, comprises a metal chlorite and a chlorite-stable antifreeze.
• the second part prior to mixing, comprises (a) an organic acid germicide and a non-esterifying antifreeze, or (b) an inorganic acid and either an alcohol or a non-esterifying antifreeze.
• the chlorite- stable and non-esterifying antifreezes contain from 4 to 16 carbon atoms and have no primary carbon atom bearing a hydroxyl group.
• the metal chlorite of the first part is an alkali or alkaline earth chlorite, such as sodium chlorite or potassium chlorite, and is typically sodium chlorite.
• the metal chlorite is present in the first part at a concentration such that, when combined with the second part, it is present within the disinfecting composition at a concentration ranging from about 0.005% to about 1% by weight, generally from 0.05% to 0.5% by weight, and typically from 0.1 % to 0.4% by weight.
• the chlorite-stable antifreeze is as disclosed above with regard to the non-esterifying antifreeze of the freeze-resistant aqueous disinfecting composition.
• the chlorite-stable antifreeze is present within the first part at a concentration ranging from about 10%» to about 75% by weight, and typically from 15% to 50% by weight of the first part.
• the second part comprises an organic acid and a non-esterifying antifreeze.
• the organic acid and the non-esterifying antifreeze of the second part is as disclosed above with regard to the organic acid and the non-esterifying acid of the freeze-resistant aqueous disinfecting composition.
• the one-part disinfecting composition may be employed as the second part of the two-part system.
• the second part comprises an inorganic acid and either an alcohol or a non-esterifying antifreeze.
• Representative inorganic acids include phosphoric acid, monosodium acid phosphate, sulfuric acid, hydrochloric acid, or sodium bisulfate.
• the inorganic acid is present in the second part at a concentration such that, when combined with the first part and before reacting therewith, it is present within the disinfecting composition at an initial concentration ranging from 0.001% to 2% by weight, and typically from 0.01% to 1.0% by weight.
• Representative alcohols include polyols, such as glycerine, sorbitol and propylene glycol, while the non-esterifying antifreeze is as disclosed above with regard to the non-esterifying antifreeze of the freeze-resistant aqueous disinfecting composition.
• the alcohol or the non-esterifying antifreeze is present within the second part at a concentration ranging from about 10% to about 75% by weight, and typically from 15% to 50%) by weight of the second part.
• the first and second parts of the two-part freeze resistant system of this invention may be independently formulated as solutions, creams or gels, and may further include one or more optional components such as a textural modifier, a surfactant, an odorant, a colorant, and mixtures thereof.
• methods are disclosed for disinfecting a substrate by contacting the substrate with an effective amount of the one-part freeze- resistant aqueous disinfecting composition of this invention, or the disinfectant composition resulting from the combination of the first and second parts of the two-part freeze-resistant disinfecting system of this invention.
• Suitable substrates in this regard include skin and, more specifically, the teat of a dairy cow.
• Freeze resistance has traditionally been imparted to aqueous systems, such as radiator coolants, by incorporation therein of such water-soluble alcohols as methanol (a mono-hydroxy compound) or glycols (which contain two alcoholic functions) such as ethylene glycol.
• methanol a mono-hydroxy compound
• glycols glycols which contain two alcoholic functions
• ethylene glycol ethylene glycol.
• Molecules which contain a greater number of hydroxyl groups referred to as "polyols”
• polyols such as glycerin and sugars
• a freeze-resistant teat dip formulation particularly one which incorporates organic acids as either active ingredients or buffers
• the above alcohols are not suitable. This is due to esterification of the hydroxyl moiety by reaction with the carboxylic acid of the organic acid.
• a material such as propylene glycol to reduce the freezing temperature of a teat dip, in which organic acids are either the germicide or the source of buffering, is counterindicated by the tendency for the acid to esterify and lose germicidal functionality.
• the lower levels of acid which result give rise to higher pH formulations, so that the remaining acid will tend to exist to a greater degree in the non- functioning anionic form.
• esterification of secondary hydroxyl groups such as the hydroxyl at the 2-position of propylene glycol
• esterification of primary alcohols such as the hydroxyl at the 1 -position of propylene-glycol
• a non-esterifying antifreeze is employed in combination with one or more organic acids.
• Such non-esterifying antifreeze agents generally contain from 4 to 16 carbon atoms, and contain one or more ether, secondary alcohol and/or ester moieties.
• Such non-esterifying antifreezes do not, however, contain any primary carbon bearing a hydoxyl group.
• a freeze-resistant aqueous disinfecting composition containing an organic acid germicide and an non-esterifying antifreeze, wherein the non-esterifying antifreeze contains from 4 to 16 carbon atoms and has no primary carbon atom bearing a hydroxyl group.
• Suitable non-esterifying antifreezes may be generally characterized as an ether having at least one ether linkage between two carbon atoms, an ether-alcohol having at least one ether linkage between two carbon atoms and with at least one secondary carbon atom bearing a hydroxyl group, or an ester having at least one ester linkage between two carbon atoms.
• Representative non-esterifying antifreezes have the structure R'-O-CH 2 -
• R 1 is C,. 8 alkyl
• R 2 and R 3 are the same or different and independently selected from hydrogen or C,. 8 alkyl, and each secondary carbon atom of the C,. 8 alkyl moiety is optionally substituted with a hydroxyl group.
• Suitable compounds are those wherein R 1 is C,. 8 alkyl moiety, R 2 is hydrogen and R 3 is C,. 8 alkyl moiety; wherein R 1 is C, .8 alkyl moiety, R 2 is hydrogen and R 3 is methyl; and wherein R 1 , R 2 and R 3 are C,. 8 alkyl.
• representative non-esterifying antifreezes have the structure R 4 -CH(OR 5 )-CH 2 -O-CH 2 -CH(OR 2 )-R 3 , wherein R 2 , R 3 , R 4 and R 5 are the same or different and independently selected from hydrogen and C,. 8 alkyl, and each secondary carbon atom of the C,. 8 alkyl moiety is optionally substituted with a hydroxyl group.
• Suitable compounds are those wherein R 4 is C,. 8 alkyl and R 5 is hydrogen; and wherein R 4 is C,. 8 alkyl and R 5 is C,. 8 alkyl.
• Non-esterifying antifreezes also include compounds of the above structures wherein at least one oxygen atom is bound to -CO(C,. 8 alkyl) to form an ester.
• More specific representative non-esterifying antifreezes of this invention include (but are not limited to) propylene glycol monomethyl ether (CH 3 - OCH 2 CH(OH)CH 3 ) propylene glycol monoethyl ether (CH 3 CH 2 -OCH 2 CH(OH)CH 3 ), propylene glycol monopropyl ether (CH 3 CH 2 CH 2 -OCH 2 CH(OH)CH 3 ), propylene glycol monoisopropyl ether (CH 3 CH(CH 3 )-OCH 2 CH(OH)CH 3 ), dipropylene glycol (CH 3 CH(OH)CH 2 OCH 2 CH(OH)CH 3 ), dipropylene glycol methyl ether (CH 3 CH(OCH 3 )CH 2 OCH 2 CH(OH)CH 3 ), dipropylene glycol ethyl ether (CH 3 CH(OCH 2 CH 3 )CH 2 OCH 2 CH(OH)CH 3 ), and dipropylene glycol acetate (CH 3 CH
• Preferred non-esterifying antifreezes are those that are soluble in water at ambient winter temperatures to at least 1 part by weight of compound per 4 parts of water. Their concentration should be from about 10% to about 75% by weight, and typically from 15% to 50%) by weight of the disinfecting composition.
• the freezing point of the disinfecting composition should be at or below 14°F (-10°C), generally below about 7°F (-14°C), and typically below about 0°F (-18°C).
• the organic acid germicide may be an alpha-hydroxy carboxylic acid having a pKa between about 2.8 and about 4.2, such as glycolic acid, lactic acid, malic acid, mandelic acid, citric acid, tartaric acid, and mixtures thereof.
• Other organic acid germicides include formic acid, acetic acid, propionic acid, benzoic acid, caprylic acid, capric acid, hydroxybenzoic acid, and mixtures thereof.
• the organic acid germicide is present at a concentration between about 0.25% and about 7.5% by weight of the disinfecting composition, and typically from 2% and 5% by weight of the disinfecting composition.
• the pH of the mixed disinfecting composition should lie in the range of about 2 to about 5, and typically from about 2.4 to about 4.5.
• organic acids such as citric
• their level of use is generally in the range of about 0.1 % to about 1.0%, where the amount utilized depends to a significant degree on the chemical characteristics of the specific formulation.
• a two-part freeze-resistant disinfecting system comprising a first part and a second part adapted to be mixed to yield an aqueous disinfecting composition.
• the first part prior to mixing, comprises a metal chlorite and a chlorite-stable antifreeze.
• the second part prior to mixing, comprises (a) an organic acid germicide and a non-esterifying antifreeze, or (b) an inorganic acid and either an alcohol or a non-esterifying antifreeze.
• the chlorite- stable and non-esterifying antifreezes contain from 4 to 16 carbon atoms and have no primary carbon atom bearing a hydroxyl group.
• the metal chlorite of the first part is an alkali or alkaline earth chlorite, such as sodium chlorite or potassium chlorite, and is typically sodium chlorite.
• the metal chlorite is present in the first part at a concentration such that, when combined with the second part, it is present within the disinfecting composition at a concentration ranging from about 0.005% to about 1% by weight, generally from 0.05% to 0.5% by weight, and typically from 0.1% to 0.4% by weight of the disinfecting composition.
• the chlorite-stable and non-esterifying antifreezes of the first and second parts, respectively, may be the same or different. Such antifreezes contain from 4 to 16 carbon atoms and have no primary carbon atom bearing a hydroxyl group. In the case of the chlorite-stable antifreeze, a primary alcohol will oxidize upon contact with chlorite, and is thus to be avoided.
• the chlorite-stable and non-esterifying antifreezes may be an ether having at least one ether linkage between two carbon atoms, an ether- alcohol having at least one ether linkage between two carbon atoms and with at least one secondary carbon atom bearing a hydroxyl group, or an ester having at least one ester linkage between two carbon atoms.
• Representative chlorite-stable and non-esterifying antifreezes have the structure R 1 -O-CH 2 -CH(OR 2 )-R 3 , wherein R 1 is C,. 8 alkyl, and R 2 and R 3 are the same or different and independently selected from hydrogen or C,. 8 alkyl, and each secondary carbon atom of the C,. 8 alkyl moiety is optionally substituted with a hydroxyl group.
• Suitable compounds are those wherein R 1 is C,. 8 alkyl moiety, R 2 is hydrogen and R 3 is C, .8 alkyl moiety; wherein R 1 is C,. 8 alkyl moiety, R 2 is hydrogen and R 3 is methyl; and wherein R 1 , R 2 and R 3 are C,.
• representative chlorite- stable and non-esterifying antifreezes have the structure R 4 -CH(OR 5 )-CH 2 -O-CH 2 - CH(OR 2 )-R 3 , wherein R 2 , R 3 , R 4 and R 5 are the same or different and independently selected from hydrogen and C,. g alkyl, and each secondary carbon atom of the C,. 8 alkyl moiety is optionally substituted with a hydroxyl group.
• Suitable compounds are those wherein R 4 is C ] . 8 alkyl and R 5 is hydrogen; and wherein R 4 is C,. 8 alkyl and R 5 is C,. 8 alkyl.
• Chlorite-stable and non-esterifying antifreezes also include compounds of the above structures wherein at least one oxygen atom is bound to -CO(C,. 8 alkyl) to form an ester.
• More specific representative chlorite-stable and non-esterifying antifreezes of this invention include (but are not limited to) propylene glycol monomethyl ether (CH 3 -OCH 2 CH(OH)CH 3 ) propylene glycol monoethyl ether (CH 3 CH 2 -OCH 2 CH(OH)CH 3 ), propylene glycol monopropyl ether (CH 3 CH 2 CH 2 - OCH 2 CH(OH)CH 3 ), propylene glycol monoisopropyl ether (CH 3 CH(CH 3 )- OCH 2 CH(OH)CH 3 ), dipropylene glycol (CH 3 CH(OH)CH 2 OCH 2 CH(OH)CH 3 ), dipropylene glycol methyl ether (CH 3 CH(OCH 3 )CH 2 OCH 2 CH(OH)CH 3 ), dipropylene glycol ethyl ether (CH 3 CH(OCH 2 CH 3 )CH 2 OCH 2 CH(OH)CH 3 ), and dipropylene glycol
• Preferred chlorite-stable and non-esterifying antifreezes are those that are soluble in water at ambient winter temperatures to at least 1 part by weight of compound per 4 parts of water. Their concentration should be from about 10% to about 75% by weight, and typically from 15% to 50% by weight of the disinfecting composition.
• the freezing point of the disinfecting composition should be at or below 14°F (-10°C), generally below about 7°F (-14°C), and typically below about 0°F (- 18°C).
• the second part comprises an organic acid and a non-esterifying antifreeze.
• the second part may be the freeze-resistant aqueous disinfecting composition as disclosed above, containing an organic acid and a non-esterifying antifreeze.
• the organic acid germicide may be an alpha-hydroxy carboxylic acid having a pKa between about 2.8 and about 4.2, such as glycolic acid, lactic acid, malic acid, mandelic acid, citric acid, tartaric acid, and mixtures thereof.
• Other organic acid germicides include formic acid, acetic acid, propionic acid, benzoic acid, caprylic acid, capric acid, hydroxybenzoic acid, and mixtures thereof.
• the organic acid germicide is present in the second part such that, following mixture with the first part, it has a concentration between about 0.25% and about 7.5%) by weight of the disinfecting composition, and typically from 2% and 5% by weight of the disinfecting composition.
• the non- esterifying antifreeze of the second part in this embodiment is as disclosed above, and is present at a concentration such that, following mixture with the first part, it is present at a concentration from about 10% to about 75% by weight, and typically from 15% to 50%) of the disinfecting composition.
• the second part comprises an inorganic acid in combination with either an alcohol or a non-esterifying antifreeze.
• the non-esterifying antifreeze of this embodiment is as disclosed above, while representative alcohols include polyols, such as glycerine, sorbitol and propylene glycol.
• Representative inorganic acids include phosphoric acid, monosodium acid phosphate, sulfuric acid, hydrochloric acid, sodium bisulfate, and mixtures thereof.
• the inorganic acid is present in the second part at a concentration such that, when combined with the first part and before reacting therewith, it is present within the disinfecting composition at an initial concentration ranging from 0.001% to 2% by weight, and typically from 0.01% to 1.0% by weight.
• the alcohol or non-esterifying antifreeze is present within the second part at a concentration such that, when combined with the first part, it is present in the disinfecting composition at a concentration from about 10% to about 75% by weight of the disinfecting composition, and typically from 15% to 50% by weight of the disinfecting composition.
• ingredients may be included in the one-part freeze resistant aqueous disinfecting composition, as well as the first part, second part, or both first and second parts of the two-part system.
• Such ingredients include (but are not limited to) wetting agents, textural modifiers, film-forming polymers, colorants and mixtures thereof.
• the wetting agents facilitate contact of the disinfecting composition with the skin, and can be selected from those materials recognized to provide this effect, in both identity and amount.
• Textural modifiers are those materials which primarily affect the body of the mixed disinfecting composition in terms of retention, flow and lubricity.
• thickening agents such as alkyl celluloses, alkoxy celluloses, xanthan gum, guar gum, and polyacrylamide derivatives, of which the polymer of 2- acrylamido-2-methylpropane sulfonic acid is a preferred example.
• Other textural modifiers include lanolin derivatives, acyl lactylates, polyethylene glycol, glyceryl esters, and mixtures thereof.
• Film-forming polymers include the above-referenced polyacrylamides, as well as the class of poly(vinyl alcohols/vinyl acetates) and polyvinyl pyrollidone. Colorants are generally selected from the group found acceptable for use in skin-contacting formulations, and are known to those skilled in the art.
• a method for disinfecting a substrate comprises contacting the substrate with an effective amount of the one-part freeze-resistant disinfecting composition of this invention, or contacting the substrate with an effective amount of the disinfecting composition formed by mixing the two-part disinfecting system of this invention.
• Suitable substrates include the skin or tissue of a warm-blooded animal and, in a preferred embodiment, the teat of a dairy cow.
• this invention is directed to a method for making a disinfecting composition
• a method for making a disinfecting composition comprising mixing the first part and the second part of the two-part disinfecting system, as well as mixing the respective compounds to form the first and second parts of the two-part system, and to form the one-part disinfectant composition.
• both the first and second parts are aqueous solutions, creams or gels.
• at least one of the first or second parts is in a concentrated form, and the concentrated form (either solid or liquid) is mixed with the other part and then diluted with water, or diluted with water and then mixed with the other part.
• This Example illustrates the preparation of a freeze-resistant germicidal formulation that remains free-flowing to below about 10°F (-12°C). It suppresses the chapping and cracking of skin to which it is applied, which might otherwise occur in sub-freezing temperatures.
• the gold-colored formulation which has a citrus odor, can kill approximately 10 4 logarithms of the microbial pathogen Staphylococcus aureus deposited onto a simulated cow teat after 1 minute of contact.
• the residual germicide on the teat surface can also destroy at least 10 2 logarithms of the environmental pathogen Streptococcus uberis 12 hours after deposition onto the teat following a 30 minute contact.
• Example 1 The above Example 1 is repeated, using 35 gms of propylene glycol monomethyl ether, 0.1 gms of methyl salicylate in place of the citral, and 0.00065 gms of methylene blue in place of the yellow and red colorants.
• the blue formulation thus prepared has a wintergreen odor, remains liquid to below about 0°F (-18°C), and has a viscosity of 305 cps.
• This example illustrates the use of the present invention in a two part chlorous acid-forming germicidal barrier teat dip, in which both parts, as well as the mixed formulation, remain fluid to a temperature below 0°F.
• a first thickened liquid is prepared by mixing the following ingredients: Coamedia brand poly (sulfonic acid), 16% solid 16.00% Sodium hydroxide, IN 16.00%
• a second thickened liquid is prepared by mixing the following ingredients:
• Poloxamer 188 0.40%
• the two thickened liquids are blended, preferably within two hours before application.
• the resulting liquid remains fluid on the cow's teats throughout the intermilking period, preventing chapping and cracking, while providing continuous antimicrobial activity to suppress mastitis formation.
• EXAMPLE 4 This example illustrates the use of the present invention in a freeze- resistant topical germicide which contains a film-forming agent and which remains fluid to below 0°F.

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• 1999
  • 1999-08-31 JP JP2000568325A patent/JP2002524397A/ja active Pending
  • 1999-08-31 WO PCT/US1999/019987 patent/WO2000013506A2/en not_active Application Discontinuation
  • 1999-08-31 KR KR1020017002745A patent/KR20010079726A/ko not_active Application Discontinuation
  • 1999-08-31 CA CA002342844A patent/CA2342844A1/en not_active Abandoned
  • 1999-08-31 EP EP99968601A patent/EP1109452A2/en not_active Withdrawn
  • 1999-08-31 BR BR9913432-2A patent/BR9913432A/pt not_active Application Discontinuation
  • 1999-08-31 MX MXPA01002282A patent/MXPA01002282A/es not_active Application Discontinuation
  • 1999-08-31 AU AU60239/99A patent/AU6023999A/en not_active Abandoned

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