EP1081215B1 - Enzyme concentrate and method for cleaning of hard surfaces - Google Patents

Enzyme concentrate and method for cleaning of hard surfaces Download PDF

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Publication number
EP1081215B1
EP1081215B1 EP99116866A EP99116866A EP1081215B1 EP 1081215 B1 EP1081215 B1 EP 1081215B1 EP 99116866 A EP99116866 A EP 99116866A EP 99116866 A EP99116866 A EP 99116866A EP 1081215 B1 EP1081215 B1 EP 1081215B1
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EP
European Patent Office
Prior art keywords
cleaning
cleaning agent
concentrate
enzyme
acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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EP99116866A
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German (de)
French (fr)
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EP1081215A1 (en
Inventor
Wolfgang Dr. Wagemann
Olaf Schreiber
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Chemische Fabrik Dr Weigert GmbH and Co
Original Assignee
WEIGERT CHEM FAB
Chemische Fabrik Dr Weigert GmbH and Co
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Priority to EP99116866A priority Critical patent/EP1081215B1/en
Priority to AT99116866T priority patent/ATE262026T1/en
Priority to DE59908892T priority patent/DE59908892D1/en
Publication of EP1081215A1 publication Critical patent/EP1081215A1/en
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Publication of EP1081215B1 publication Critical patent/EP1081215B1/en
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    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38618—Protease or amylase in liquid compositions only
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/20—Organic compounds containing oxygen
    • C11D3/2075—Carboxylic acids-salts thereof
    • C11D3/2086—Hydroxy carboxylic acids-salts thereof
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/26—Organic compounds containing nitrogen
    • C11D3/30—Amines; Substituted amines ; Quaternized amines
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38663—Stabilised liquid enzyme compositions
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D2111/00—Cleaning compositions characterised by the objects to be cleaned; Cleaning compositions characterised by non-standard cleaning or washing processes
    • C11D2111/10—Objects to be cleaned
    • C11D2111/14—Hard surfaces
    • C11D2111/20—Industrial or commercial equipment, e.g. reactors, tubes or engines

Definitions

  • the invention relates to a method for cleaning contaminated with milk or milk residues Surfaces.
  • DE-A-196 40 201 therefore chooses the way out, an enzymatic one Cleaner either in a powder form that is difficult to dose provide or in the form of two separate Liquid components that are added separately shortly before use become. This is expensive.
  • US-A-5,571,446 also uses for reasons of stability two separate liquid concentrates, in WO-A-97/07190 for an enzymatic cleaner called the problematic powder form selected.
  • DE-A-197 17 329 discloses a liquid enzymatic cleaner, by the choice of certain solvents and by addition of polyhexamethylene biguanide or N, N-bis (3-aminopropyl) dodecylamine is stabilized. This causes additional Costs.
  • US-A-4,212,761 discloses a method for cleaning milking installations or the like in which a slightly alkaline cleaning solution is used, the tensides and proteinase contains.
  • the cleaner is considered a difficult to dose powder made available.
  • EP-A-0,806,472 discloses that stubborn tannin covers have dishes removed by a two-stage cleaning process, in the first with an organic acid or pre-cleaned with a complexing agent and then main cleaning with an enzymatic cleaning solution.
  • DE-A-3741617 discloses a liquid dishwashing detergent which has a stabilized enzyme system.
  • an enzyme stabilizer is a mixture of a Mur compound, an alpha hydroxy carboxylic acid and casein used.
  • the invention has for its object a method of to create a good cleaning effect compared with proteins and / or fat (especially Milk or milk residues) has contaminated surfaces and is easy to use in practice.
  • the invention solves this problem by the features of Main claim.
  • a cleaner concentrate is a concentrated preparation that dilutes with water the usable cleaning solution results.
  • the use concentrations of the concentrate in one finished cleaning solution are 0.1 to 10 wt .-%, in particular 0.2 to 5% by weight.
  • the liquid concentrate is stable when stored under the Practical conditions stored at least a few months can be without the enzyme activity in the cleaning performance decreases significantly.
  • Buffer substances work in the pH range greater than 9 to 12, if itself when preparing the cleaning solution with water and the usual Amount of concentrate in this solution is the stated pH from over 9 to a maximum of 12.
  • Proteolytic enzymes are enzymes from the group of proteases (Peptidases).
  • the one used to prepare the concentrate Water is preferably common tap water contains a small amount of calcium ions.
  • it can be tap water with about 10 to 25 ° dH, in particular about 15 ° dH. Demineralized water can also be used become.
  • the invention is based on the surprising finding that proteolytic enzymes in alkaline formulations of here disclosed compositions for a made-up application can be sufficiently stabilized.
  • a cleaning concentrate is made available that is sufficiently stable in storage without any special effort, is easy to dose due to its liquid form and at the practical application not adding two or more Components for the production of the finished cleaning solution required.
  • the water content of the concentrate is preferably at most 62% by weight, more preferably at most 60% by weight.
  • the the lower limit of the water content is preferably at least 30% by weight, more preferably at least 35% by weight it is preferably over 40% by weight.
  • Particularly preferred is within the scope of the invention a water content between 41 and 60 wt .-%, more preferred is a content between 50 and 60% by weight.
  • the proteolytic enzymes are preferably selected from the Group consisting of Properase, Savinase and Esperase. A Combination of several proteolytic enzymes can be used become.
  • the concentrate preferably contains 1 to 10% by weight, more preferably 3 to 6% by weight of enzyme preparation.
  • Enzyme preparations are those of the relevant Manufacturers related enzymatic compositions referred.
  • Properase 1600 L from Genencor is available, for example (Enzyme activity of the commercial preparation 1600 PU), Savinase 16.0 L and Esperase 8.0 L from Novo Nordisk (Enzyme activities 16 and 8 KNPU).
  • the buffer substances of the concentrate work preferably in the pH range 9.2 to 11.5, more preferably 9.5 to 11, more preferably 9.5 to 10.5. It should usually such a pH range can be set in which the used Enzymes have an optimum effect.
  • the buffer substances preferably have at least one combination an organic amine and at least one organic Acid on.
  • the organic amine can be an alkanolamine be, for example mono-, di- and / or triethanolamine (MEA, DEA, TEA).
  • Inorganic buffer systems (phosphate buffer or the like) can also be used.
  • the organic acid can be a single or polybasic acid be two to seven carbon atoms, e.g. a fruit acid such as lemon, Malic and / or tartaric acid.
  • Claim 1 is not a final list of the components of the cleaner concentrate. It can in addition to water, other solvents such as ethylene glycol, Contain propylene glycol or glycerin. solubilizers such as cumene sulfonate, xylene sulfonate, or octyl sulfonate also be used, their share in the concentrate is preferably about 1 to 10% by weight.
  • Surfactants anionic, cationic, nonionic or amphoteric Surfactants
  • defoaming agents can also be added become.
  • Suitable surfactants are, for example, N-alkylpyrrolidones, which are preferred be added in a proportion of 0.1 to 2 wt .-%.
  • the preferred length of the alkyl chain is 8 to 12 carbon atoms.
  • Nonionic surfactants which also act as defoamers, can adducts of ethylene oxide and propylene oxide, for example Be fatty alcohols.
  • Ethylene oxide / propylene oxide adducts are preferred of fatty alcohols.
  • the commercially available ones may be mentioned as examples Block adducts of 2 to 5 mol on average Ethylene oxide and 2 to 4 moles of propylene oxide with 1 mole of fatty alcohols with 8 to 18, in particular 8 to 14 carbon atoms. she can in the concentrate in an amount of 1 to 15, in particular 2 to 12 wt .-% may be included.
  • Complexing agents or other means of regulating water hardness can also be added. Named as an example be chelating agents such as NTA, EDTA or hydroxyethylaminodimethylenephosphonic acid (HEADMP). Polymers such as polyacrylic acid or copolymers thereof can also be used become.
  • chelating agents such as NTA, EDTA or hydroxyethylaminodimethylenephosphonic acid (HEADMP).
  • Polymers such as polyacrylic acid or copolymers thereof can also be used become.
  • the inventive method is characterized in that the (especially with milk and / or milk or milk product residues) contaminated surfaces with a acidic cleaners are rinsed, then done main cleaning with an enzyme-containing enzyme as defined in claim 1 Cleanser.
  • the invention has recognized that acidic pre-rinsing is the result the subsequent main enzymatic cleaning surprisingly significantly improved and / or accelerated.
  • the method according to the invention can be carried out in the context of a so-called CIP cleaning (cleaning in place).
  • CIP cleaning cleaning in place
  • the method of removing stubborn is particularly suitable Residues on the heat exchanger surfaces of milk heaters.
  • the method according to the invention can also be used cleaning any surface (especially hard Surfaces of metal, glass or the like.) In the food or beverage industry.
  • the pre-rinsing preferably takes place for a period of 2 to 60 min, preferably 5 to 30 min, particularly preferred about 15 min at a temperature from room temperature to 70 ° C, preferably 40 to 65 ° C, particularly preferably about 60 ° C.
  • the Prewash solution preferably contains about 0.1 to 2% by weight mineral acid dissolved in water, more preferably the mineral acid content 0.5 to 1% by weight. It can, for example, nitric acid or use phosphoric acid.
  • the main cleaning step takes place under Use of the enzyme-containing cleaner concentrate.
  • the duration of the main cleaning is preferably between 5 and 120 min, more preferably between 10 to 60 min, particularly preferably about 45 min.
  • the temperature in the Main purification step is chosen so that the enzymes are Can develop cleaning activity. It can be between room temperature and about 65 ° C, preferably 30 to 60 ° C, particularly preferably about 45 to 60 ° C.
  • the first step is to check the cleaning performance a test stain is required that corresponds to the actual Conditions (especially in milk heaters) equivalent.
  • a test stain is required that corresponds to the actual Conditions (especially in milk heaters) equivalent.
  • the prior art according to DE-A-197 17 329 just dry condensed milk on stainless steel plates, this only leads to contamination with fats, but not with heat-denatured proteins.
  • Circular stainless steel plate elements are used as substrates with a diameter of 50 mm and a wall thickness of 1 mm used. These are in a rectangular channel with a Gap cross section of 2.55 mm used. Fresh raw milk is preheated to a temperature of 65 ° C and with a Flow rate of about 3 mm / s pumped into the gap.
  • the rectangular channel itself is from the outside by means of a separate heater acted on with heat, so that there is a temperature in it of 95 ° C.
  • the plate elements remain for 3.5 hours in the rectangular channel, then they are removed.
  • the plate elements have one on the product side semi-transparent, gray-green, rubber-elastic covering, the the air dries quickly and a firm bond with the Metal surface forms.
  • The is in the dried state Surface brittle and so hard that it cannot be touched with a fingernail can be scratched.
  • Fig. 1 shows such a plate element after applying the Test soil.
  • a plate element provided with the test soiling is cleaned in the manner described with a cleaning solution, containing 5 g / l NaOH dissolved in water. With this solution the plate element is at a temperature for 45 min cleaned from 65 ° C. The cleaning result is in Fig. 2 shown. Clear residues of covering can be seen.
  • This Cleaning the usual cleaning of dairy plants in practice with an alkaline cleaner as a reference for the assessment of those described below cleaning experiments according to the invention.
  • the acidic pre-rinse is carried out with a 0.5% aqueous solution of nitric acid at 60 ° C for a period of 15 min.
  • the intermediate rinsing is carried out with Tap water for a period of 2 min.
  • the cleaner concentrate according to Example 1 is diluted of 0.5 wt .-% used.
  • aqueous cleaning solution has a pH of about 10 a.
  • the main cleaning takes place at one temperature of 50 ° C for a period of 45 min.
  • the cleaning result is shown in Fig. 3.
  • the cleaner concentrate according to Example 2 is in one concentration of 0.5% by weight with tap water to a cleaning solution stated.
  • the solution has a pH of 9.5 a.
  • the main cleaning takes place at a temperature of 55 ° C for a period of 45 min.
  • the cleaning result can be seen from Fig. 4.
  • the cleaner concentrate according to Example 3 is with tap water in a concentration of 0.5% by weight to a cleaning solution in which a pH of 9.2 is established.
  • the main cleaning takes place at a temperature of 60 ° C for a period of 45 min.
  • the cleaning result is in Fig. 5 shown.

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
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  • Enzymes And Modification Thereof (AREA)

Abstract

Liquid enzymatic cleaner concentrate with good storage stability comprises a buffer effective at pH 9-12, a proteolytic enzyme and at most 65 wt.% water.

Description

Die Erfindung betrifft ein ein Verfahren zum Reinigen von mit Milch oder milchrückständen verunreinigten Oberflächen.The invention relates to a method for cleaning contaminated with milk or milk residues Surfaces.

Auf Oberflächen von Behältern und Leitungen, die bei der Milchgewinnung und Milchverarbeitung mit Milch oder Milchprodukten in Berührung kommen, bilden sich Proteine und Fette enthaltende Ablagerungen, die häufig nur schwer zu entfernen sind. Derartige Ablagerungen finden sich bspw. in Melkmaschinen, Milchsammelwagen, Tanks, Rohrleitungen und in besonders starkem Maße in Milcherhitzern. Zur Reinigung solcher Oberflächen werden häufig stark alkalische Reiniger verwendet, deren Handhabung besondere Vorsichtsmaßnahmen erfordert und die außerdem korrosiv wirken können.On surfaces of containers and lines used in the Milk production and processing with milk or milk products When they come into contact, proteins and fats are formed containing deposits that are often difficult to remove are. Such deposits can be found, for example, in milking machines, Milk collection vehicles, tanks, pipes and in particular strong in milk heaters. For cleaning such surfaces strong alkaline cleaners are often used, the handling of which requires special precautionary measures and which can also be corrosive.

Es ist daher bereits vorgeschlagen worden, enzymatische Reinigerzusammensetzungen zur Reinigung derartiger Oberflächen zu verwenden. Erstrebenswert ist es, solche Reiniger in Form flüssiger Konzentrate zur Verfügung zu stellen, da sich diese im praktischen Betrieb am einfachsten handhaben und dosieren lassen. Flüssige Enzymzubereitungen sind jedoch häufig nicht lagerstabil. It has therefore already been proposed to use enzymatic detergent compositions for cleaning such surfaces to use. It is desirable to have such cleaners in shape to provide liquid concentrates as these easiest to handle and dose in practical operation to let. However, liquid enzyme preparations are often not storage stable.

DE-A-196 40 201 wählt daher den Ausweg, einen enzymatischen Reiniger entweder in einer nur schwierig zu dosierenden Pulverform bereitzustellen oder aber in Form zweier separater Flüssigkomponenten, die kurz vor dem Einsatz separat zudosiert werden. Dies ist aufwendig.DE-A-196 40 201 therefore chooses the way out, an enzymatic one Cleaner either in a powder form that is difficult to dose provide or in the form of two separate Liquid components that are added separately shortly before use become. This is expensive.

US-A-5,571,446 verwendet aus Stabilitätsgründen ebenfalls zwei getrennte Flüssigkonzentrate, in WO-A-97/07190 wird für einen enzymatischen Reiniger die genannte problematische Pulverform gewählt.US-A-5,571,446 also uses for reasons of stability two separate liquid concentrates, in WO-A-97/07190 for an enzymatic cleaner called the problematic powder form selected.

DE-A-197 17 329 offenbart einen flüssigen enzymatischen Reiniger, der durch wahl bestimmter Lösungsmittel und durch Zusatz von Polyhexamethylenbiguanid oder N,N-Bis(3-aminopropyl)dodecylamin stabilisiert ist. Dies verursacht zusätzliche Kosten.DE-A-197 17 329 discloses a liquid enzymatic cleaner, by the choice of certain solvents and by addition of polyhexamethylene biguanide or N, N-bis (3-aminopropyl) dodecylamine is stabilized. This causes additional Costs.

US-A-4,212,761 offenbart ein Verfahren zum Reinigen von Melkanlagen oder dergleichen, bei dem eine leicht alkalische Reinigungslösung zum Einsatz kommt, die Tenside und Proteinase enthält. Der Reiniger wird als schwierig zu dosierendes Pulver zur Verfügung gestellt.US-A-4,212,761 discloses a method for cleaning milking installations or the like in which a slightly alkaline cleaning solution is used, the tensides and proteinase contains. The cleaner is considered a difficult to dose powder made available.

EP-A-0,806,472 offenbart, daß sich hartnäckige Gerbstoffbelege von Geschirr entfernen lassen durch ein zweistufiges Reinigungsverfahren, bei dem zunächst mit einer organischen Säure oder einem Komplexbildner vorgereinigt und anschließend mit einer enzymatischen Reinigerlösung hauptgereinigt wird.EP-A-0,806,472 discloses that stubborn tannin covers have dishes removed by a two-stage cleaning process, in the first with an organic acid or pre-cleaned with a complexing agent and then main cleaning with an enzymatic cleaning solution.

DE-A-3741617 offenbart ein flüssiges Geschirrwaschmittel, das ein stabilisiertes Enzymsystem aufweist. Als Enzymstabilisator wird eine Mischung aus einer Murverbindung, einer Alphahydroxicarbonsäure und Kasein verwendet.DE-A-3741617 discloses a liquid dishwashing detergent which has a stabilized enzyme system. As an enzyme stabilizer is a mixture of a Mur compound, an alpha hydroxy carboxylic acid and casein used.

Der Erfindung liegt die Aufgabe zugrunde, ein Verfahren der eingangs genannten Art zu schaffen, das eine gute Reinigungswirkung gegenüber mit Proteinen und/oder Fett (insbesondere Milch bzw. Milchrückstände) verunreinigten Oberflächen aufweistund in der Praxis einfach anwendbar ist.The invention has for its object a method of to create a good cleaning effect compared with proteins and / or fat (especially Milk or milk residues) has contaminated surfaces and is easy to use in practice.

Die Erfindung löst diese Aufgabe durch die Merkmale des Hauptanspruchs.The invention solves this problem by the features of Main claim.

Gegenstand der Erfindung ist somit ein Verfahren zum Reinigen von verunreinigten Oberflächen mit den Schritten:

  • a) zur Verfügung stellen eines flüssigen enzymatischen Reinigerkonzentrats, das enthält:
    • im pH-Bereich größer 9 bis 12 wirkende Puffersubstanzen,
    • wenigstens ein im pH-Bereich größer 9 bis 12 wirkendes proteolytisches Enzym,
    • Wasser in einem Anteil von 30 bis 65 Gew.-%.
  • b) Vorspülen der Oberflächen mit einem sauren Reiniger,
  • c) Hauptreinigen mit einem enzymhaltigen Reiniger, der 0,1 bis 10 Gew.-% des Reinigerkonzentrats aus Schritt a) in wäßriger Lösung enthält.
    • The invention thus relates to a method for cleaning contaminated surfaces with the steps:
  • a) providing a liquid enzymatic cleaner concentrate which contains:
    • buffer substances acting in the pH range greater than 9 to 12,
    • at least one proteolytic enzyme acting in the pH range greater than 9 to 12,
    • Water in a proportion of 30 to 65 wt .-%.
  • b) rinsing the surfaces with an acidic cleaner,
  • c) Main cleaning with an enzyme-containing cleaner which contains 0.1 to 10% by weight of the cleaner concentrate from step a) in aqueous solution.

    • Zunächst seien einige im Rahmen der Erfindung verwendete Begriffe erläutert. First of all, let us consider some terms used in the context of the invention explained.

    Ein Reinigerkonzentrat ist eine konzentrierte Zubereitung, die mit Wasser verdünnt die einsatzfähige Reinigungslösung ergibt. Die Einsatzkonzentrationen des Konzentrats in einer fertigen Reinigerlösung sind 0,1 bis 10 Gew.-%, insbesondere 0,2 bis 5 Gew.-%.A cleaner concentrate is a concentrated preparation that dilutes with water the usable cleaning solution results. The use concentrations of the concentrate in one finished cleaning solution are 0.1 to 10 wt .-%, in particular 0.2 to 5% by weight.

    Das flüssige Konzentrat ist lagerstabil, wenn es unter in der Praxis üblichen Bedingungen zumindest einige Monate gelagert werden kann, ohne daß die Enzymaktivität in einer die Reinigungsleistung wesentlich beeinträchtigenden Weise abnimmt. The liquid concentrate is stable when stored under the Practical conditions stored at least a few months can be without the enzyme activity in the cleaning performance decreases significantly.

    Puffersubstanzen wirken im pH-Bereich größer 9 bis 12, wenn sich beim Ansetzen der Reinigerlösung mit Wasser und der üblichen Menge Konzentrat in dieser Lösung der genannte pH-Wert von über 9 bis höchstens 12 einstellt.Buffer substances work in the pH range greater than 9 to 12, if itself when preparing the cleaning solution with water and the usual Amount of concentrate in this solution is the stated pH from over 9 to a maximum of 12.

    Proteolytische Enzyme sind Enzyme aus der Gruppe der Proteasen (Peptidasen). Das zur Zubereitung des Konzentrats eingesetzte Wasser ist vorzugsweise übliches Leitungswasser, das einen geringen Anteil Calciumionen enthält. Bspw. kann es sich um Leitungswasser mit etwa 10 bis 25°dH handeln, insbesondere etwa 15°dH. Vollentsalztes Wasser kann ebenfalls verwendet werden.Proteolytic enzymes are enzymes from the group of proteases (Peptidases). The one used to prepare the concentrate Water is preferably common tap water contains a small amount of calcium ions. For example. it can are tap water with about 10 to 25 ° dH, in particular about 15 ° dH. Demineralized water can also be used become.

    Die Erfindung beruht auf der überraschenden Erkenntnis, daß proteolytische Enzyme in alkalischen Formulierungen der hier offengelegten Zusammensetzungen für eine konfektionierte Anwendung ausreichend stabilisiert werden können. Es wird so ein Reinigerkonzentrat zur Verfügung gestellt, das ohne besonderen Aufwand hinreichend lagerstabil ist, durch seine flüssige Form einfach zu dosieren ist und bei der praktischen Anwendung nicht das Zudosieren von zwei oder mehr Komponenten zur Herstellung der fertigen Reinigerlösung erfordert.The invention is based on the surprising finding that proteolytic enzymes in alkaline formulations of here disclosed compositions for a made-up application can be sufficiently stabilized. A cleaning concentrate is made available that is sufficiently stable in storage without any special effort, is easy to dose due to its liquid form and at the practical application not adding two or more Components for the production of the finished cleaning solution required.

    Der Wassergehalt des Konzentrats beträgt vorzugsweise höchstens 62 Gew.-%, weiter vorzugsweise höchstens 60 Gew.-%. Die untere Grenze des Wassergehalts liegt bevorzugt bei mindestes 30 Gew.-%, weiter vorzugsweise bei mindestens 35 Gew.-%, weiter vorzugsweise beträgt sie über 40 Gew.-%. Besonders bevorzugt ist im Rahmen der Erfindung ein Wassergehalt zwischen 41 und 60 Gew.-%, weiter bevorzugt ist ein Gehalt zwischen 50 und 60 Gew.-%. The water content of the concentrate is preferably at most 62% by weight, more preferably at most 60% by weight. The the lower limit of the water content is preferably at least 30% by weight, more preferably at least 35% by weight it is preferably over 40% by weight. Particularly preferred is within the scope of the invention a water content between 41 and 60 wt .-%, more preferred is a content between 50 and 60% by weight.

    Die proteolytischen Enzyme sind bevorzugt ausgewählt aus der Gruppe bestehend aus Properase, Savinase und Esperase. Eine Kombination mehrerer proteolytischer Enzyme kann verwendet werden. Das Konzentrat enthält bevorzugt 1 bis 10 Gew.-%, weiter vorzugsweise 3 bis 6 Gew.-% Enzymzubereitung. Mit "Enzymzubereitungen" werden dabei die von den einschlägigen Herstellern bezogenen enzymatischen Zusammensetzungen bezeichnet. Erhältlich ist bspw. Properase 1600 L der Firma Genencor (Enzymaktivität der käuflichen Zubereitung 1600 PU), Savinase 16.0 L und Esperase 8.0 L der Firma Novo Nordisk (Enzymaktivitäten 16 bzw. 8 KNPU).The proteolytic enzymes are preferably selected from the Group consisting of Properase, Savinase and Esperase. A Combination of several proteolytic enzymes can be used become. The concentrate preferably contains 1 to 10% by weight, more preferably 3 to 6% by weight of enzyme preparation. With "Enzyme preparations" are those of the relevant Manufacturers related enzymatic compositions referred. Properase 1600 L from Genencor is available, for example (Enzyme activity of the commercial preparation 1600 PU), Savinase 16.0 L and Esperase 8.0 L from Novo Nordisk (Enzyme activities 16 and 8 KNPU).

    Die Puffersubstanzen des Konzentrats wirken bevorzugt im pH-Bereich 9,2 bis 11,5, weiter bevorzugt 9,5 bis 11, weiter bevorzugt 9,5 bis 10,5. Es soll in der Regel ein solcher pH-Bereich eingestellt werden, in dem die verwendeten Enzyme ein Wirkungsoptimum aufweisen.The buffer substances of the concentrate work preferably in the pH range 9.2 to 11.5, more preferably 9.5 to 11, more preferably 9.5 to 10.5. It should usually such a pH range can be set in which the used Enzymes have an optimum effect.

    Bevorzugt weisen die Puffersubstanzen eine Kombination wenigstens eines organischen Amins und wenigstens einer organischen Säure auf. Das organische Amin kann ein Alkanolamin sein, bspw. Mono-, Di- und/oder Triethanolamin (MEA, DEA, TEA). Anorganische Puffersysteme (Phosphatpuffer oder dergl.) können ebenfalls verwendet werden.The buffer substances preferably have at least one combination an organic amine and at least one organic Acid on. The organic amine can be an alkanolamine be, for example mono-, di- and / or triethanolamine (MEA, DEA, TEA). Inorganic buffer systems (phosphate buffer or the like) can also be used.

    Die organische Säure kann eine ein- oder mehrbasige Säure mit zwei bis sieben C-Atomen sein, bspw. eine Fruchtsäure wie Zitronen-, Äpfel- und/oder Weinsäure.The organic acid can be a single or polybasic acid be two to seven carbon atoms, e.g. a fruit acid such as lemon, Malic and / or tartaric acid.

    Anspruch 1 stellt keine abschließende Aufzählung der Bestandteile des Reinigerkonzentrats dar. Es können neben Wasser weitere Lösungsmittel wie bspw. Ethylenglycol, Propylenglycol oder Glycerin enthalten. Lösungsvermittler wie Cumolsulfonat, Xylolsulfonat, oder Octylsulfonat können ebenfalls eingesetzt werden, ihr Anteil am Konzentrat beträgt vorzugsweise etwa 1 bis 10 Gew.-%.Claim 1 is not a final list of the components of the cleaner concentrate. It can in addition to water, other solvents such as ethylene glycol, Contain propylene glycol or glycerin. solubilizers such as cumene sulfonate, xylene sulfonate, or octyl sulfonate also be used, their share in the concentrate is preferably about 1 to 10% by weight.

    Tenside (anionische, kationische, nichtionische oder amphotere Tenside) sowie Entschäumungsmittel können ebenfalls zugesetzt werden.Surfactants (anionic, cationic, nonionic or amphoteric Surfactants) and defoaming agents can also be added become.

    Geeignete Tenside sind bspw. N-Alkylpyrrolidone, die bevorzugt in einem Anteil von 0,1 bis 2 Gew.-% zugesetzt werden. Die bevorzugte Länge der Alkylkette beträgt 8 bis 12 C-Atome.Suitable surfactants are, for example, N-alkylpyrrolidones, which are preferred be added in a proportion of 0.1 to 2 wt .-%. The preferred length of the alkyl chain is 8 to 12 carbon atoms.

    Nichtionische Tenside, die gleichzeitig als Entschäumer wirken, können bspw. Addukte von Ethylenoxid und Propylenoxid an Fettalkohole sein. Bevorzugt sind Ethylenoxid/Propylenoxidaddukte an Fettalkohole. Beispielhaft genannt seien die handelsüblichen Blockaddukte von im Zahlenmittel 2 bis 5 Mol Ethylenoxid und 2 bis 4 Mol Propylenoxid an 1 Mol Fettalkohole mit 8 bis 18, insbesondere 8 bis 14 Kohlenstoffatomen. Sie können im Konzentrat in einer Menge von 1 bis 15, insbesondere 2 bis 12 Gew.-% enthalten sein.Nonionic surfactants, which also act as defoamers, can adducts of ethylene oxide and propylene oxide, for example Be fatty alcohols. Ethylene oxide / propylene oxide adducts are preferred of fatty alcohols. The commercially available ones may be mentioned as examples Block adducts of 2 to 5 mol on average Ethylene oxide and 2 to 4 moles of propylene oxide with 1 mole of fatty alcohols with 8 to 18, in particular 8 to 14 carbon atoms. she can in the concentrate in an amount of 1 to 15, in particular 2 to 12 wt .-% may be included.

    Komplexbildner oder andere Mittel zur Regulierung der Wasserhärte können ebenfalls zugesetzt werden. Beispielhaft genannt seien Chelatbildner wie NTA, EDTA oder auch Hydroxyethylaminodimethylenphosphonsäure (HEADMP). Polymere wie bspw. Polyacrylsäure oder Copolymere daraus können ebenfalls eingesetzt werden. Complexing agents or other means of regulating water hardness can also be added. Named as an example be chelating agents such as NTA, EDTA or hydroxyethylaminodimethylenephosphonic acid (HEADMP). Polymers such as polyacrylic acid or copolymers thereof can also be used become.

    Das erfindungsgemäße Verfahren zeichnet sich dadurch aus, daß die (insbesondere mit Milch und/oder Milch- bzw. Milchproduktrückständen) verunreinigten Oberflächen zunächst mit einem sauren Reiniger vorgespült werden, anschließend erfolgt ein Hauptreinigen mit einem in Anspruch 1 definierten enzymhaltigen Reiniger.The inventive method is characterized in that the (especially with milk and / or milk or milk product residues) contaminated surfaces with a acidic cleaners are rinsed, then done main cleaning with an enzyme-containing enzyme as defined in claim 1 Cleanser.

    Die Erfindung hat erkannt, daß das saure Vorspülen das Ergebnis der anschließenden enzymatischen Hauptreinigung überraschenderweise wesentlich verbessert und/oder beschleunigt.The invention has recognized that acidic pre-rinsing is the result the subsequent main enzymatic cleaning surprisingly significantly improved and / or accelerated.

    Das erfindungsgemäße Verfahren kann im Rahmen einer sogenannten CIP-Reinigung (cleaning in place) durchgeführt werden. Besonders geeignet ist das Verfahren zum Entfernen hartnäckiger Rückstände auf den Wärmetauscheroberflächen von Milcherhitzern. Das erfindungsgemäße Verfahren kann jedoch auch bei der Reinigung jeglicher Oberflächen (insbesondere harter Oberflächen aus Metall, Glas oder dergl.) in der Lebensmittel- oder Getränkeindustrie angewendet werden.The method according to the invention can be carried out in the context of a so-called CIP cleaning (cleaning in place). The method of removing stubborn is particularly suitable Residues on the heat exchanger surfaces of milk heaters. However, the method according to the invention can also be used cleaning any surface (especially hard Surfaces of metal, glass or the like.) In the food or beverage industry.

    Das Vorspülen geschieht vorzugsweise für einem Zeitraum von 2 bis 60 min, vorzugsweise 5 bis 30 min, besonders bevorzugt etwa 15 min bei einer Temperatur von Raumtemperatur bis 70°C, bevorzugt 40 bis 65°C, besonders bevorzugt etwa 60°C. Die Vorspüllösung enthält vorzugsweise etwa 0,1 bis 2 Gew.-% Mineralsäure gelöst in Wasser, weiter bevorzugt beträgt der Mineralsäuregehalt 0,5 bis 1 Gew.-%. Es kann bspw. Salpetersäure oder Phosphorsäure Verwendung finden.The pre-rinsing preferably takes place for a period of 2 to 60 min, preferably 5 to 30 min, particularly preferred about 15 min at a temperature from room temperature to 70 ° C, preferably 40 to 65 ° C, particularly preferably about 60 ° C. The Prewash solution preferably contains about 0.1 to 2% by weight mineral acid dissolved in water, more preferably the mineral acid content 0.5 to 1% by weight. It can, for example, nitric acid or use phosphoric acid.

    Der Hauptreinigungsschritt erfolgt unter Verwendung des enzymhaltigen Reinigerkonzentrats. Die Dauer der Hauptreinigung liegt bevorzugt zwischen 5 und 120 min, weiter vorzugsweise zwischen 10 bis 60 min, besonders bevorzugt etwa 45 min. Die Temperatur im Hauptreinigungsschritt wird so gewählt, daß die Enzyme ihre Reinigungsaktivität entfalten können. Sie kann zwischen Raumtemperatur und etwa 65°C liegen, bevorzugt 30 bis 60°C, besonders bevorzugt etwa 45 bis 60°C.The main cleaning step takes place under Use of the enzyme-containing cleaner concentrate. The duration of the main cleaning is preferably between 5 and 120 min, more preferably between 10 to 60 min, particularly preferably about 45 min. The temperature in the Main purification step is chosen so that the enzymes are Can develop cleaning activity. It can be between room temperature and about 65 ° C, preferably 30 to 60 ° C, particularly preferably about 45 to 60 ° C.

    Zwischen Vorspülen und Hauptreinigen kann ein Zwischenspülen zur Entfernung von Säureresten erfolgen. Dies ist insbesondere dann von Vorteil, wenn die Pufferkapazität des verwendeten Hauptreinigers nicht ausreicht, um aus dem Vorspülschritt verbleibende Säurereste abzufangen und den pH-Wert im Hauptreinigungsschritt auf den für die gewünschte Enzymaktivität erforderlichen Wert einzustellen.There can be an intermediate rinse between pre-rinsing and main cleaning to remove acid residues. This is particularly so then advantageous if the buffer capacity of the used Main cleaner is insufficient to get out of the pre-rinse step intercept remaining acid residues and the pH in Main cleaning step on the for the desired enzyme activity set the required value.

    Nachfolgend werden Ausführungsbeispiele der Erfindung erläutert. In den Zeichnungen zeigen:

    Fig. 1
    ein für die Reinigungsversuche verwendetes Plattenelement mit einem Belag erhitzter Milchrückstände,
    Fig. 2
    ein solches Plattenelement nach der Reinigung mit einem alkalischen Reiniger (Vergleichsversuch),
    Fig. 3 bis 5
    ein solches Plattenelement nach der Reinigung mit dem erfindungsgemäßen Verfahren unter Verwendung drei verschiedener Reinigerkonzentrate.
    Exemplary embodiments of the invention are explained below. The drawings show:
    Fig. 1
    a plate element used for cleaning tests with a coating of heated milk residues,
    Fig. 2
    such a plate element after cleaning with an alkaline cleaner (comparative experiment),
    3 to 5
    Such a plate element after cleaning with the method according to the invention using three different cleaner concentrates.

    Für die Prüfung der Reinigungsleistung ist zunächst die Herstellung einer Testanschmutzung erforderlich, die den tatsächlichen Gegebenheiten (insbesondere in Milcherhitzern) entspricht. Bspw. läßt der Stand der Technik gemäß DE-A-197 17 329 lediglich Kondensmilch auf Edelstahlplättchen antrocknen, dadurch kommt es lediglich zu einer Verunreinigung mit Fetten, nicht jedoch mit durch Hitze denaturierten Proteinen.The first step is to check the cleaning performance a test stain is required that corresponds to the actual Conditions (especially in milk heaters) equivalent. For example. the prior art according to DE-A-197 17 329 just dry condensed milk on stainless steel plates, this only leads to contamination with fats, but not with heat-denatured proteins.

    Herstellung der TestanschmutzungPreparation of the test soil

    Als Substrate werden kreisförmige Edelstahlplattenelemente mit einem Durchmesser von 50 mm und einer Wandstärke von 1 mm verwendet. Diese werden in einem Rechteckkanal mit einem Spaltquerschnitt von 2.55 mm eingesetzt. Frische Rohmilch wird auf eine Temperatur von 65°C vorgewärmt und mit einer Fließrate von etwa 3 mm/s in den Spalt gepumpt. Der Rechteckkanal selbst wird von außen mittels einer separaten Heizung mit Wärme beaufschlagt, so daß sich in ihm eine Temperatur von 95°C einstellt.Circular stainless steel plate elements are used as substrates with a diameter of 50 mm and a wall thickness of 1 mm used. These are in a rectangular channel with a Gap cross section of 2.55 mm used. Fresh raw milk is preheated to a temperature of 65 ° C and with a Flow rate of about 3 mm / s pumped into the gap. The rectangular channel itself is from the outside by means of a separate heater acted on with heat, so that there is a temperature in it of 95 ° C.

    Unter diesen Bedingungen verbleiben die Plattenelemente für 3,5 h in dem Rechteckkanal, anschließend werden sie herausgenommen. Die Plattenelemente haben auf der Produktseite einen halbtransparenten, graugrünen, gummielastischen Belag, der an der Luft schnell trocknet und einen festen Verbund mit der Metalloberfläche bildet. Im angetrockneten zustand ist der Belag spröde und so hart, daß er mit dem Fingernagel nicht angeritzt werden kann.Under these conditions, the plate elements remain for 3.5 hours in the rectangular channel, then they are removed. The plate elements have one on the product side semi-transparent, gray-green, rubber-elastic covering, the the air dries quickly and a firm bond with the Metal surface forms. The is in the dried state Surface brittle and so hard that it cannot be touched with a fingernail can be scratched.

    Fig. 1 zeigt ein solches Plattenelement nach Aufbringen der Testanschmutzung. Fig. 1 shows such a plate element after applying the Test soil.

    Weitere Einzelheiten zur Aufbringung der Testanschmutzung sind beschrieben in Kieler Milchwirtschaftliche Forschungsberichte 35 (4), 493-519(1983) sowie in Milchwissenschaft 43 (12)1988. Die Offenbarung dieser Veröffentlichungen wird durch Bezugnahme darauf zum Gegenstand auch der vorliegenden Anmeldung gemacht.More details on applying test soil are described in Kiel Dairy Research Reports 35 (4), 493-519 (1983) and in milk science 43 (12) 1988th The disclosure of these publications will by reference to the subject matter of the present Registration made.

    Herstellung der ReinigerkonzentrateProduction of cleaner concentrates

    Die in Tabelle 1 angegebenen Inhaltsstoffe werden zu einem Reinigerkonzentrat angesetzt. Bei dem Wasser handelt es sich um Hamburger Stadtwasser mit etwa 15°dH. Alle %-Angaben in Tabelle 1 sind Gew.-%. Die Rohstoffbezeichnungen sind teilweise die dem Fachmann geläufigen INCI-Namen. Rohstoff Beispiel 1 Beispiel 2 Beispiel 3 Wasser 46,22 44,3 44,3 PAS 4000-Na,45% 0,2 0,2 0,2 Zitronensäure 1,68 0,4 0,4 MEA 4,8 3 3 TEA 3 8 8 HEADMP 0,2 0,2 0,2 Na-Cumolsulfonat, 40% 18 18 18 1,2-Propylenglykol 10 10 10 Octylpyrrolidon 0,9 0,9 0,9 Laureth-10-n Butylether 5 Coceth-5 n-Butylether 5 PPG-4,5 Laureth-4 5 PPG-4 Laureth-2 5 PPG-4 Laureth-5 5 Octeth-4 5 Properase 1600 5 Savinase 16 5 Esperase 8.0 5 Gesamtwassergehalt 59,9 55,7 56,6 The ingredients listed in Table 1 are made up into a cleaner concentrate. The water is Hamburg city water with about 15 ° dH. All% data in Table 1 are% by weight. The raw material names are partly the INCI names familiar to the expert. raw material example 1 Example 2 Example 3 water 46.22 44.3 44.3 PAS 4000-Na, 45% 0.2 0.2 0.2 citric acid 1.68 0.4 0.4 MEA 4.8 3 3 TEA 3 8th 8th HEADMP 0.2 0.2 0.2 Na cumene sulfonate, 40% 18 18 18 1,2-propylene glycol 10 10 10 -octylpyrrolidone 0.9 0.9 0.9 Laureth-10-n butyl ether 5 Coceth-5 n-butyl ether 5 PPG-4.5 Laureth-4 5 PPG-4 Laureth-2 5 PPG-4 Laureth-5 5 Octeth-4 5 Properase 1600 5 Savinase 16 5 Esperase 8.0 5 Total water content 59.9 55.7 56.6

    Reinigungsversuchecleaning tests

    Sämtliche Reinigungsversuche erfolgen in einem Fließkanal mit einem rechteckigen Querschnitt von 365 mm. In diesem Kanal werden die verunreinigten Plattenelemente eingesetzt, er wird mit der jeweils verwendeten Reinigerlösung beaufschlagt. Die Strömungsgeschwindigkeit im Kanal während der Reinigungsversuche beträgt 1 m/s. Damit sollen übliche Bedingungen bei CIP-Reinigen von Molkereianlagen simuliert werden.All cleaning attempts are carried out in a flow channel a rectangular cross section of 365 mm. In this channel the contaminated plate elements are used, he will charged with the detergent solution used in each case. The Flow velocity in the channel during the cleaning attempts is 1 m / s. This is supposed to be the usual conditions CIP cleaning of dairy plants can be simulated.

    Vergleichsbeispiel:Comparative Example:

    Ein mit der Testanschmutzung versehenes Plattenelement wird auf die beschriebene Weise mit einer Reinigerlösung gereinigt, die 5 g/l NaOH gelöst in Wasser enthält. Mit dieser Lösung wird das Plattenelement während 45 min bei einer Temperatur von 65°C gereinigt. Das Reinigungsergebnis ist in Fig. 2 gezeigt. Deutliche Belagreste sind zu erkennen. Diese Reinigung, die der in der Praxis üblichen Reinigung von Molkereianlagen mit einem alkalischen Reiniger entspricht, dient als Referenz für die Beurteilung der nachfolgend beschriebenen erfindungsgemäßen Reinigungsversuche.A plate element provided with the test soiling is cleaned in the manner described with a cleaning solution, containing 5 g / l NaOH dissolved in water. With this solution the plate element is at a temperature for 45 min cleaned from 65 ° C. The cleaning result is in Fig. 2 shown. Clear residues of covering can be seen. This Cleaning, the usual cleaning of dairy plants in practice with an alkaline cleaner as a reference for the assessment of those described below cleaning experiments according to the invention.

    Beispiele 4 bis 6Examples 4 to 6

    Diese erfindungsgemäßen Reinigungsbeispiele werden nach dem erfindungsgemäßen Verfahren mit einer sauren Vorspülung, gefolgt von einem Zwischenspülen mit Wasser und einem Hauptreinigen unter Verwendung eines Reinigerkonzentrats durchgeführt.These cleaning examples according to the invention are after Process according to the invention followed by an acidic pre-rinse an intermediate rinse with water and a main wash using a detergent concentrate carried out.

    Bei sämtlichen Beispielen erfolgt das saure Vorspülen mit einer 0,5%igen wäßrigen Lösung von Salpetersäure bei 60°C für einen Zeitraum von 15 min. Die Zwischenspülung erfolgt mit Leitungswasser für einen Zeitraum von 2 min.In all examples, the acidic pre-rinse is carried out with a 0.5% aqueous solution of nitric acid at 60 ° C for a period of 15 min. The intermediate rinsing is carried out with Tap water for a period of 2 min.

    Beispiel 4Example 4

    Das Reinigerkonzentrat gemäß Beispiel 1 wird in einer Verdünnung von 0,5 Gew.-% eingesetzt. In der mit dem Konzentrat angesetzten wäßrigen Reinigerlösung stellt sich ein pH-Wert von etwa 10 ein. Das Hauptreinigen erfolgt bei einer Temperatur von 50°C für einen Zeitraum von 45 min. Das Reinigungsergebnis ist in Fig. 3 gezeigt.The cleaner concentrate according to Example 1 is diluted of 0.5 wt .-% used. In the one prepared with the concentrate aqueous cleaning solution has a pH of about 10 a. The main cleaning takes place at one temperature of 50 ° C for a period of 45 min. The cleaning result is shown in Fig. 3.

    Beispiel 5Example 5

    Das Reinigerkonzentrat gemäß Beispiel 2 wird in einer Konzentration von 0,5 Gew.-% mit Leitungswasser zu einer Reinigerlösung angesetzt. In der Lösung stellt sich ein pH-Wert von 9,5 ein. Das Hauptreinigen erfolgt bei einer Temperatur von 55°C für einen Zeitraum von 45 min. Das Reinigungsergebnis ist aus Fig. 4 ersichtlich.The cleaner concentrate according to Example 2 is in one concentration of 0.5% by weight with tap water to a cleaning solution stated. The solution has a pH of 9.5 a. The main cleaning takes place at a temperature of 55 ° C for a period of 45 min. The cleaning result can be seen from Fig. 4.

    Beispiel 6Example 6

    Das Reinigerkonzentrat gemäß Beispiel 3 wird mit Leitungswasser in einer Konzentration von 0,5 Gew.-% zu einer Reinigerlösung angesetzt, in der sich ein pH-Wert von 9,2 einstellt.The cleaner concentrate according to Example 3 is with tap water in a concentration of 0.5% by weight to a cleaning solution in which a pH of 9.2 is established.

    Das Hauptreinigen erfolgt bei einer Temperatur von 60°C für einen Zeitraum von 45 min. Das Reinigungsergebnis ist in Fig. 5 gezeigt.The main cleaning takes place at a temperature of 60 ° C for a period of 45 min. The cleaning result is in Fig. 5 shown.

    Die Beispiele belegen, daß sich erfindungsgemäß eine praktisch vollständige Ablösung hartnäckiger, erhitzter Milchrückstände in einem CIP-Reinigungsverfahren erreichen läßt.The examples show that, according to the invention, a practical complete removal of stubborn, heated milk residues can be achieved in a CIP cleaning process.

    Claims (17)

    1. A method for cleaning surfaces contaminated with milk and/or milk residues, having the steps:
      a) providing a liquid enzymatic cleaning agent concentrate, which contains:
      buffer substances active over a pH range of greater than 9 to 12,
      at least one proteolytic enzyme active over a pH range of greater than 9 to 12,
      water in a proportion of 30 to 65 wt.%
      b) prerinsing the surfaces with an acidic cleaning agent,
      c) main cleaning with an enzyme-containing cleaning agent, which contains 0.1 to 10 wt.% of the cleaning agent concentrate from step a) in aqueous solution.
    2. A method according to claim 1, characterised in that prerinsing is performed using an aqueous solution which contains 0.1 to 2 wt.%, preferably 0.5 to 1 wt.%, of mineral acid.
    3. A method according to claim 1 or 2, characterised in that intermediate rinsing is performed between prerinsing and main cleaning.
    4. A method according to one of claims 1 to 3, characterised in that the surfaces are contaminated with milk residues.
    5. A method according to one of claims 1 to 4, characterised in that the enzyme-containing cleaning agent contains 0.1 to 10 wt.%, preferably 0.2 to 5 wt.%, of enzyme concentrate.
    6. A method according to one of claims 1 to 5, characterised in that the water content of the cleaning agent concentrate amounts to at most 62 wt.%, preferably at most 60 wt.%.
    7. A method according to one of claims 1 to 6, characterised in that the water content of the cleaning agent concentrate amounts to at least 35 wt.%, preferably over 40 wt.%.
    8. A method according to claim 7, characterised in that the water content of the cleaning agent concentrate is between 41 and 60 wt.%, preferably 50 to 60 wt.%.
    9. A method according to one of claims 1 to 8, characterised in that the proteolytic enzyme of the cleaning agent concentrate is selected from the group consisting of Properase, Savinase and Esperase.
    10. A method according to one of claims 1 to 9, characterised in that the cleaning agent concentrate contains 1 to 10 wt.%, preferably 3 to 6 wt.%, of enzyme preparation.
    11. A method according to one of claims 1 to 10, characterised in that the buffer substances of the cleaning agent concentrate are active in the pH range 9.2 to 11.5, preferably 9.5 to 11, more preferably 9.5 to 10.5.
    12. A method according to one of claims 1 to 11, characterised in that the buffer substances of the cleaning agent concentrate comprise a combination of at least one organic amine and at least one organic acid.
    13. A method according to claim 12, characterised in that the organic amine is an alkanolamine.
    14. A method according to claim 13, characterised in that the alkanolamine is selected from the group consisting of mono-, di- and triethanolamine.
    15. A method according to one of claims 12 to 14, characterised in that the organic acid is a mono- or polybasic acid with 2 to 7 C atoms.
    16. A method according to claim 15, characterised in that the organic acid is a fruit acid.
    17. A method according to claim 16, characterised in that the fruit acid is selected from the group consisting of citric, malic and tartaric acid.
    EP99116866A 1999-09-02 1999-09-02 Enzyme concentrate and method for cleaning of hard surfaces Expired - Lifetime EP1081215B1 (en)

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    EP99116866A EP1081215B1 (en) 1999-09-02 1999-09-02 Enzyme concentrate and method for cleaning of hard surfaces
    AT99116866T ATE262026T1 (en) 1999-09-02 1999-09-02 ENZYME CONCENTRATE AND METHOD FOR CLEANING SURFACES
    DE59908892T DE59908892D1 (en) 1999-09-02 1999-09-02 Enzyme concentrate and method for cleaning surfaces

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    US8328951B2 (en) 2009-04-01 2012-12-11 Henkel Ag & Co. Kgaa Cleaning agent

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    US6472199B1 (en) * 2001-04-04 2002-10-29 West Agro, Inc. Method of cleaning dairy pipelines using enzyme pretreatment
    AR061906A1 (en) * 2006-07-18 2008-10-01 Novapharm Res Australia LOW FOAM CLEANER
    DE102013218449A1 (en) 2013-09-13 2015-03-19 Schülke & Mayr GmbH Aqueous formulation for cleaning hard surfaces
    WO2017177016A1 (en) * 2016-04-06 2017-10-12 Cinder Biological, Inc. Enzymatic cleaning and sanitizing compositions and methods of using the same

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    US4212761A (en) * 1978-03-06 1980-07-15 Novo Laboratories, Inc. Method and composition for cleaning dairy equipment
    GB2200132B (en) * 1986-12-15 1991-09-18 Colgate Palmolive Co Stabilised enzyme systems and compositions containing them
    AU3667189A (en) * 1988-06-23 1990-01-04 Unilever Plc Enzyme-containing liquid detergents
    EP0450702A3 (en) * 1990-04-06 1993-06-02 Unilever N.V. Process for preparing liquid enzymatic detergent compositions
    US5073292A (en) * 1990-06-07 1991-12-17 Lever Brothers Company, Division Of Conopco, Inc. Heavy duty liquid detergent compositions containing enzymes stabilized by quaternary nitrogen substituted proteins
    US5510052A (en) * 1994-08-25 1996-04-23 Colgate-Palmolive Co. Enzymatic aqueous pretreatment composition for dishware
    DE19618725A1 (en) * 1996-05-09 1997-11-13 Weigert Chem Fab Method and kit for cleaning dishes

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    Publication number Priority date Publication date Assignee Title
    US8328951B2 (en) 2009-04-01 2012-12-11 Henkel Ag & Co. Kgaa Cleaning agent

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