EP1040201A4 - Single chain monoclonal antibody fusion reagents that regulate transcription in vivo - Google Patents

Single chain monoclonal antibody fusion reagents that regulate transcription in vivo

Info

Publication number
EP1040201A4
EP1040201A4 EP97949553A EP97949553A EP1040201A4 EP 1040201 A4 EP1040201 A4 EP 1040201A4 EP 97949553 A EP97949553 A EP 97949553A EP 97949553 A EP97949553 A EP 97949553A EP 1040201 A4 EP1040201 A4 EP 1040201A4
Authority
EP
European Patent Office
Prior art keywords
vivo
monoclonal antibody
single chain
antibody fusion
regulate transcription
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP97949553A
Other languages
German (de)
French (fr)
Other versions
EP1040201A1 (en
Inventor
James P Hoeffler
Marijane Russell
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Publication of EP1040201A1 publication Critical patent/EP1040201A1/en
Publication of EP1040201A4 publication Critical patent/EP1040201A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
EP97949553A 1997-11-28 1997-11-28 Single chain monoclonal antibody fusion reagents that regulate transcription in vivo Withdrawn EP1040201A4 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1997/021407 WO1999028502A1 (en) 1997-11-28 1997-11-28 Single chain monoclonal antibody fusion reagents that regulate transcription in vivo

Publications (2)

Publication Number Publication Date
EP1040201A1 EP1040201A1 (en) 2000-10-04
EP1040201A4 true EP1040201A4 (en) 2001-11-21

Family

ID=22262117

Family Applications (1)

Application Number Title Priority Date Filing Date
EP97949553A Withdrawn EP1040201A4 (en) 1997-11-28 1997-11-28 Single chain monoclonal antibody fusion reagents that regulate transcription in vivo

Country Status (3)

Country Link
EP (1) EP1040201A4 (en)
AU (1) AU3587599A (en)
WO (1) WO1999028502A1 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1479694B1 (en) * 1999-12-28 2016-10-26 ESBATech, an Alcon Biomedical Research Unit LLC Intrabodies ScFv with defined framework that is stable in a reducing environment
US6696620B2 (en) 2000-05-02 2004-02-24 Epicyte Pharmaceutical, Inc. Immunoglobulin binding protein arrays in eukaryotic cells
US6410246B1 (en) * 2000-06-23 2002-06-25 Genetastix Corporation Highly diverse library of yeast expression vectors
US6410271B1 (en) * 2000-06-23 2002-06-25 Genetastix Corporation Generation of highly diverse library of expression vectors via homologous recombination in yeast
US6406863B1 (en) * 2000-06-23 2002-06-18 Genetastix Corporation High throughput generation and screening of fully human antibody repertoire in yeast
US6610472B1 (en) * 2000-10-31 2003-08-26 Genetastix Corporation Assembly and screening of highly complex and fully human antibody repertoire in yeast
US7005503B2 (en) 2002-02-08 2006-02-28 Genetastix Corporation Human monoclonal antibody against coreceptors for human immunodeficiency virus
US7138496B2 (en) 2002-02-08 2006-11-21 Genetastix Corporation Human monoclonal antibodies against human CXCR4
EP1363931A4 (en) * 2001-02-01 2004-04-07 Tanox Inc Methods to generate and identify monoclonal antibodies to a large number of human antigens
ATE434040T1 (en) 2001-10-01 2009-07-15 Dyax Corp MULTI-CHAIN EUKARYONTIC DISPLAY VECTORS AND USES THEREOF
WO2003066830A2 (en) * 2002-02-08 2003-08-14 Genetastix Corporation Human monoclonal antibodies against membrane proteins
HUE048922T2 (en) 2002-05-22 2020-09-28 Novartis Ag Immunoglobulin frameworks which demonstrate enhanced stability in the intracellular environment and methods of identifying same
US20040067532A1 (en) 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
BRPI0816785A2 (en) 2007-09-14 2017-05-02 Adimab Inc rationally designed synthetic antibody libraries, and uses thereof
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
KR101323827B1 (en) * 2010-01-08 2013-10-31 키스트 유럽 에프게엠베하 Primers for diagnosing ankylosing spondylitis, and method for diagnosing ankylosing spondylitis using the same
MX360336B (en) 2010-07-16 2018-10-30 Adimab Llc Star Antibody libraries.
CN108060232A (en) * 2017-12-13 2018-05-22 安徽师范大学 For expanding tortoise androgen receptor（AR）The RT-qPCR primers and method of gene

Citations (5)

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Publication number Priority date Publication date Assignee Title
EP0557897A1 (en) * 1992-02-28 1993-09-01 F. Hoffmann-La Roche Ag Antigen binding proteins and methods for their production
WO1996002561A1 (en) * 1994-07-20 1996-02-01 The General Hospital Corporation Interaction trap systems for detecting protein interactions
WO1996030512A1 (en) * 1995-03-31 1996-10-03 Rhone-Poulenc Rorer S.A. Conditional expression system
WO2000054057A1 (en) * 1999-03-10 2000-09-14 Medical Research Council Selection of intracellular immunoglobulins
WO2001048017A1 (en) * 1999-12-28 2001-07-05 Esbatech Ag Intrabodies with defined framework that is stable in a reducing environment and applications thereof

Family Cites Families (4)

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US5525491A (en) * 1991-02-27 1996-06-11 Creative Biomolecules, Inc. Serine-rich peptide linkers
WO1994021294A1 (en) * 1993-03-19 1994-09-29 Bigner Darell D Method of treating tumors with antibodies
US5610013A (en) * 1993-07-22 1997-03-11 Ludwig Institute For Cancer Research Method for diagnosing a disorder by determining expression of gage tumor rejection antigen precursors
US5658763A (en) * 1993-10-25 1997-08-19 Creative Biomolecules, Inc. Methods and compositions for high protein production from non-native DNA

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0557897A1 (en) * 1992-02-28 1993-09-01 F. Hoffmann-La Roche Ag Antigen binding proteins and methods for their production
WO1996002561A1 (en) * 1994-07-20 1996-02-01 The General Hospital Corporation Interaction trap systems for detecting protein interactions
WO1996030512A1 (en) * 1995-03-31 1996-10-03 Rhone-Poulenc Rorer S.A. Conditional expression system
WO2000054057A1 (en) * 1999-03-10 2000-09-14 Medical Research Council Selection of intracellular immunoglobulins
WO2001048017A1 (en) * 1999-12-28 2001-07-05 Esbatech Ag Intrabodies with defined framework that is stable in a reducing environment and applications thereof

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
DE JAEGER GEERT ET AL: "Analysis of the interaction between single-chain variable fragments and their antigen in a reducing intracellular environment using the two-hybrid system", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 467, no. 2-3, 11 February 2000 (2000-02-11), pages 316 - 320, XP002143443, ISSN: 0014-5793 *
GARGANO N ET AL: "FROM PHAGE LIBRARIES TO INTRACELLULAR IMMUNIZATION", INTRACELLULAR ANTIBODIES: DEVELOPMENT AND APPLICATIONS, SPRINGER VERLAG, BERLIN,, DE, 1997, pages 173 - 186, XP000916894 *
HAARD DE H J W ET AL: "SELECTION OF RECOMBINANT, LIBRARY-DERIVED ANTIBODY FRAGMENTS AGAINST P24 FOR HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 DIAGNOSTICS", CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 5, no. 5, September 1998 (1998-09-01), pages 636 - 644, XP000939091, ISSN: 1071-412X *
MARASCO W A: "Intracellular antibodies (intrabodies) as research reagents and therapeutic molecules for gene therapy", IMMUNOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS BV, NL, vol. 1, no. 1, 1 May 1995 (1995-05-01), pages 1 - 19, XP004052680, ISSN: 1380-2933 *
MARASCO W: "Intrabodies: turning the humoral immune system outside in for intracellular immunization", GENE THERAPY, MACMILLAN PRESS LTD., BASINGSTOKE, GB, vol. 4, no. 1, January 1997 (1997-01-01), pages 11 - 15, XP002097642, ISSN: 0969-7128 *
MHASHILKAR A M ET AL: "Inhibition of HIV-1 Tat-mediated LTR transactivation and HIV-1 infection by anti- Tat single chain intrabodies", EMBO JOURNAL, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 14, no. 7, 1995, pages 1542 - 1551, XP002089075, ISSN: 0261-4189 *
PORTNER-TALIANA A ET AL: "In vivo selection of single-chain antibodies using a yeast two-hybrid system", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 238, no. 1-2, April 2000 (2000-04-01), pages 161 - 172, XP004195472, ISSN: 0022-1759 *
See also references of WO9928502A1 *
VISINTIN MICHELA ET AL: "Selection of antibodies for intracellular function using a two-hybrid in vivo system", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 96, no. 21, 12 October 1999 (1999-10-12), pages 11723 - 11728, XP002143442, ISSN: 0027-8424 *

Also Published As

Publication number Publication date
AU3587599A (en) 1999-06-16
WO1999028502A1 (en) 1999-06-10
EP1040201A1 (en) 2000-10-04

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