EP0976834A3 - Liquid reagent for calcium assay - Google Patents

Liquid reagent for calcium assay Download PDF

Info

Publication number
EP0976834A3
EP0976834A3 EP99114742A EP99114742A EP0976834A3 EP 0976834 A3 EP0976834 A3 EP 0976834A3 EP 99114742 A EP99114742 A EP 99114742A EP 99114742 A EP99114742 A EP 99114742A EP 0976834 A3 EP0976834 A3 EP 0976834A3
Authority
EP
European Patent Office
Prior art keywords
reagent
calcium
calcium ion
activity
assay
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP99114742A
Other languages
German (de)
French (fr)
Other versions
EP0976834A2 (en
EP0976834B1 (en
Inventor
Hirokazu Matsukawa
Osamu Oka
Tuyosi Fujita
Hitoshi Sakakibara
Tatsuo Sugiyama
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oriental Yeast Co Ltd
Original Assignee
Oriental Yeast Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oriental Yeast Co Ltd filed Critical Oriental Yeast Co Ltd
Publication of EP0976834A2 publication Critical patent/EP0976834A2/en
Publication of EP0976834A3 publication Critical patent/EP0976834A3/en
Application granted granted Critical
Publication of EP0976834B1 publication Critical patent/EP0976834B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01002—Glutamate dehydrogenase (1.4.1.2)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/04—Endocrine or metabolic disorders

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Inorganic Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to an enzyme immunoassay method for assaying calcium ion, including putting calcium-dependent glutamate dehydrogenase, namely glutamate dehydrogenase (GLDH) never expressing its activity in the absence of calcium ion, in contact to the calcium ion in a sample to assay the enzyme activity of the GLDH, the activity changing depending on the calcium ion concentration in the sample; and the invention also relates to an assay reagent for use in the same. Because the reagent is very stable in a solution state under storage, the reagent can be incorporated in a liquid reagent. Thus, the calcium in a biological material or other samples can be assayed in a simple and accurate manner.
EP99114742A 1998-07-30 1999-07-28 Liquid reagent for calcium assay Expired - Lifetime EP0976834B1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP22856598 1998-07-30
JP22856598 1998-07-30
JP14517399 1999-05-25
JP14517399A JP4416866B2 (en) 1998-07-30 1999-05-25 Liquid reagent for calcium measurement

Publications (3)

Publication Number Publication Date
EP0976834A2 EP0976834A2 (en) 2000-02-02
EP0976834A3 true EP0976834A3 (en) 2000-06-14
EP0976834B1 EP0976834B1 (en) 2004-03-10

Family

ID=26476373

Family Applications (1)

Application Number Title Priority Date Filing Date
EP99114742A Expired - Lifetime EP0976834B1 (en) 1998-07-30 1999-07-28 Liquid reagent for calcium assay

Country Status (4)

Country Link
US (2) US20020120952A1 (en)
EP (1) EP0976834B1 (en)
JP (1) JP4416866B2 (en)
DE (1) DE69915416T2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104951898B (en) * 2015-07-02 2017-02-22 北京理工大学 Task-oriented cooperative multi-agent coalition formation method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0035831A2 (en) * 1980-03-07 1981-09-16 Imperial Chemical Industries Plc Method for making genetically modified microorganisms
WO1987006264A1 (en) * 1986-04-16 1987-10-22 Public Health Research Institute Of The City Of Ne Expression of the cloned lysostaphin gene
EP0555046A1 (en) * 1992-02-07 1993-08-11 Oriental Yeast Co., Ltd. Method for determination of calcium

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR920010313B1 (en) * 1987-04-10 1992-11-26 더 플린더스 유니버시티 오브 사우쓰 오스트레일리아 Determination of sodium ions in fluids

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0035831A2 (en) * 1980-03-07 1981-09-16 Imperial Chemical Industries Plc Method for making genetically modified microorganisms
WO1987006264A1 (en) * 1986-04-16 1987-10-22 Public Health Research Institute Of The City Of Ne Expression of the cloned lysostaphin gene
EP0555046A1 (en) * 1992-02-07 1993-08-11 Oriental Yeast Co., Ltd. Method for determination of calcium

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, vol. 123, no. 21, 20 November 1995, Columbus, Ohio, US; abstract no. 277526, H. SAKAKIBARA ET AL.: "Isolation and characterization of a cDNA that encodes maize glutamate dehydrogenase" page 310; column 1; XP002135439 *
M. P. PURNELL ET AL.: "Cloning and characterisation of a glutamate dehydrogenase cDNA from tomato (Lycopersicon esculentum L.)", GENE: AN INTERNATIONAL JOURNAL ON GENES AND GENOMES., vol. 186, 1997, ELSEVIER SCIENCE PUBLISHERS, BARKING., GB, pages 249 - 254, XP004056137, ISSN: 0378-1119 *
PLANT CELL PHYSIOLOGY, vol. 36, no. 5, 1995, pages 789 - 797 *

Also Published As

Publication number Publication date
DE69915416D1 (en) 2004-04-15
US20040014163A1 (en) 2004-01-22
JP4416866B2 (en) 2010-02-17
US20020120952A1 (en) 2002-08-29
EP0976834A2 (en) 2000-02-02
DE69915416T2 (en) 2005-01-13
JP2000102392A (en) 2000-04-11
EP0976834B1 (en) 2004-03-10

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