EP0902836A4 - Asymmetric hammerhead ribozymes - Google Patents

Asymmetric hammerhead ribozymes

Info

Publication number
EP0902836A4
EP0902836A4 EP97913997A EP97913997A EP0902836A4 EP 0902836 A4 EP0902836 A4 EP 0902836A4 EP 97913997 A EP97913997 A EP 97913997A EP 97913997 A EP97913997 A EP 97913997A EP 0902836 A4 EP0902836 A4 EP 0902836A4
Authority
EP
European Patent Office
Prior art keywords
compound
hammerhead ribozymes
man
asymmetric
asymmetric hammerhead
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP97913997A
Other languages
German (de)
French (fr)
Other versions
EP0902836A1 (en
Inventor
Philip Hendry
Maxine J Mccall
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gene Shears Pty Ltd
Original Assignee
Gene Shears Pty Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gene Shears Pty Ltd filed Critical Gene Shears Pty Ltd
Publication of EP0902836A1 publication Critical patent/EP0902836A1/en
Publication of EP0902836A4 publication Critical patent/EP0902836A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
    • C12N2310/121—Hammerhead
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar
    • C12N2310/321—2'-O-R Modification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

This invention is directed to a compound having the formula (I), wherein N may be a nucleotide, n is from 1-5 and n' is from 1-3. Such asymmetric ribozymes have higher than normal cleavage rates. The compound may be covalently linked to a delivery agent. The invention also includes a composition which comprises the compound in association with an acceptable carrier. The invention also includes a method of cleaving an RNA target sequence with the compound as described above. Further, a method of treating a disease in man or animals associated with a particular RNA which comprises administrating to the man or animal the compound. Further, the invention also includes a diagnostic reagent which comprises the compound.
EP97913997A 1996-04-02 1997-04-02 Asymmetric hammerhead ribozymes Withdrawn EP0902836A4 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US62703396A 1996-04-02 1996-04-02
US1465996P 1996-04-02 1996-04-02
US627033 1996-04-02
US14659P 1996-04-02
PCT/AU1997/000210 WO1997037013A1 (en) 1996-04-02 1997-04-02 Asymmetric hammerhead ribozymes

Publications (2)

Publication Number Publication Date
EP0902836A1 EP0902836A1 (en) 1999-03-24
EP0902836A4 true EP0902836A4 (en) 2004-12-22

Family

ID=26686341

Family Applications (1)

Application Number Title Priority Date Filing Date
EP97913997A Withdrawn EP0902836A4 (en) 1996-04-02 1997-04-02 Asymmetric hammerhead ribozymes

Country Status (5)

Country Link
EP (1) EP0902836A4 (en)
JP (1) JP2000509969A (en)
AU (1) AU721758B2 (en)
CA (1) CA2250857A1 (en)
WO (1) WO1997037013A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004512810A (en) * 1999-08-31 2004-04-30 サーナ・セラピューティクス・インコーポレイテッド Modulators of gene expression based on nucleic acids
AU781151B2 (en) * 1999-10-26 2005-05-12 Immusol Incorporated Ribozyme therapy for the treatment of proliferative skin and eye diseases
GB0224663D0 (en) * 2002-10-23 2002-12-04 Isis Innovation DNAzyme

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991019789A1 (en) * 1990-06-19 1991-12-26 Commonwealth Scientific And Industrial Research Organisation Endonucleases

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08500481A (en) * 1992-05-11 1996-01-23 リボザイム・ファーマシューティカルズ・インコーポレーテッド Methods and agents for inhibiting viral replication
AU687001B2 (en) * 1992-05-14 1998-02-19 Ribozyme Pharmaceuticals, Inc. Method and reagent for inhibiting cancer development
JPH07509133A (en) * 1992-07-17 1995-10-12 リボザイム・ファーマシューティカルズ・インコーポレイテッド Methods and agents for the treatment of animal diseases
AU5334294A (en) * 1993-10-15 1995-05-04 Dkfz Deutsches Krebsforschungszentrum Stiftung Des Offentlichen Rechts Asymmetric hammerhead ribozymes and nucleotide sequences for their construction

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991019789A1 (en) * 1990-06-19 1991-12-26 Commonwealth Scientific And Industrial Research Organisation Endonucleases

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
AMIRI KHALED M A ET AL: "Global conformation of a self-cleaving hammerhead RNA", BIOCHEMISTRY, vol. 33, no. 45, 1994, pages 13172 - 13177, XP002303024, ISSN: 0006-2960 *
DENMAN ROBERT B ET AL: "Differential activity of trans-acting hammerhead ribozymes targeted to beta amyloid peptide precursor mRNA by altering the symmetry of helices I and III", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, vol. 323, no. 1, 1995, pages 71 - 78, XP002303023, ISSN: 0003-9861 *
HENDRY PHILIP ET AL: "A comparison of the in vitro activity of DNA-armed and all-RNA hammerhead ribozymes", NUCLEIC ACIDS RESEARCH, vol. 23, no. 19, 1995, pages 3928 - 3936, XP002303026, ISSN: 0305-1048 *
HENDRY PHILIP ET AL: "Unexpected anisotropy in substrate cleavage rates by asymmetric hammerhead ribozymes", NUCLEIC ACIDS RESEARCH, vol. 24, no. 14, 15 July 1996 (1996-07-15), pages 2679 - 2684, XP002303027, ISSN: 0305-1048 *
See also references of WO9737013A1 *
TABLER M ET AL: "A THREE-NUCLEOTIDE HELIX I IS SUFFICIENT FOR FULL ACTIVITY OF A HAMMERHEAD ROBOZYME: ADVANTAGES OF AN ASYMMETRIC DESIGN", NUCLEIC ACIDS RESEARCH, vol. 22, no. 19, 1994, pages 3958 - 3965, XP002051226, ISSN: 0305-1048 *
ZOUMADAKIS M ET AL: "Comparative analysis of cleavage rates after systematic permutation of the NUX consensus target motif for hammerhead ribozymes.", NUCLEIC ACIDS RESEARCH, vol. 23, no. 7, 11 April 1995 (1995-04-11), pages 1192 - 1196, XP002303025, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
JP2000509969A (en) 2000-08-08
EP0902836A1 (en) 1999-03-24
WO1997037013A1 (en) 1997-10-09
AU721758B2 (en) 2000-07-13
CA2250857A1 (en) 1997-10-09
AU2145097A (en) 1997-10-22

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Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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17P Request for examination filed

Effective date: 19981102

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): DE FR GB IT

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: GENE SHEARS PTY LIMITED

A4 Supplementary search report drawn up and despatched

Effective date: 20041108

17Q First examination report despatched

Effective date: 20050126

17Q First examination report despatched

Effective date: 20050126

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20071215