EP0814156A3 - Method for the purification of DNA - Google Patents
Method for the purification of DNA Download PDFInfo
- Publication number
- EP0814156A3 EP0814156A3 EP97109708A EP97109708A EP0814156A3 EP 0814156 A3 EP0814156 A3 EP 0814156A3 EP 97109708 A EP97109708 A EP 97109708A EP 97109708 A EP97109708 A EP 97109708A EP 0814156 A3 EP0814156 A3 EP 0814156A3
- Authority
- EP
- European Patent Office
- Prior art keywords
- dna
- carrier
- lysing
- cells
- column
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP157245/96 | 1996-06-18 | ||
JP15724596 | 1996-06-18 | ||
JP15724596 | 1996-06-18 | ||
JP26149796 | 1996-10-02 | ||
JP26149796 | 1996-10-02 | ||
JP261497/96 | 1996-10-02 |
Publications (3)
Publication Number | Publication Date |
---|---|
EP0814156A2 EP0814156A2 (en) | 1997-12-29 |
EP0814156A3 true EP0814156A3 (en) | 1998-04-22 |
EP0814156B1 EP0814156B1 (en) | 2003-03-05 |
Family
ID=26484773
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP97109708A Expired - Lifetime EP0814156B1 (en) | 1996-06-18 | 1997-06-14 | Method for the purification of DNA |
Country Status (4)
Country | Link |
---|---|
US (1) | US5916775A (en) |
EP (1) | EP0814156B1 (en) |
CA (1) | CA2207852A1 (en) |
DE (1) | DE69719409T2 (en) |
Families Citing this family (28)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7026468B2 (en) | 1996-07-19 | 2006-04-11 | Valentis, Inc. | Process and equipment for plasmid purification |
US7807822B2 (en) | 1996-08-01 | 2010-10-05 | Robert Bridenbaugh | Methods for purifying nucleic acids |
DE19746874A1 (en) * | 1997-10-23 | 1999-04-29 | Qiagen Gmbh | Isolation of nucleic acids |
US7569342B2 (en) | 1997-12-10 | 2009-08-04 | Sierra Molecular Corp. | Removal of molecular assay interferences |
US6844158B1 (en) | 1997-12-22 | 2005-01-18 | Hitachi Chemical Co., Ltd. | Direct RT-PCR on oligonucleotide-immobilized PCR microplates |
ATE401415T1 (en) * | 1997-12-22 | 2008-08-15 | Hitachi Chemical Co Ltd | DIRECT RT-PCR ON MICROTITER PLATES WITH IMMOBILIZED OLIGONUCLEOTIDE |
US6291179B1 (en) * | 1998-09-10 | 2001-09-18 | Whatman Plc | Product and method for separation of a sample containing multiple sources of genetic material using a solid medium |
AU6114199A (en) * | 1998-10-09 | 2000-05-01 | Whatman Bioscience Limited | Isolation method for nucleic acid and apparatus |
US6958392B2 (en) | 1998-10-09 | 2005-10-25 | Whatman, Inc. | Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products |
GB9822141D0 (en) * | 1998-10-09 | 1998-12-02 | Cambridge Molecular Tech | Isolation method and apparatus |
WO2000034457A1 (en) * | 1998-12-10 | 2000-06-15 | Takara Shuzo Co., Ltd. | Method for immobilizing oligonucleotide on a carrier |
GB2355717A (en) * | 1999-10-28 | 2001-05-02 | Amersham Pharm Biotech Uk Ltd | DNA isolation method |
GB2363381B (en) * | 2000-06-12 | 2004-11-24 | Cambridge Molecular Tech | Solution |
ATE309344T1 (en) * | 2001-01-19 | 2005-11-15 | Mueller Drm Ag | METHOD FOR PREPARING BIOMASS FOR PRODUCING CELL LYSATE CONTAINING PLASMID DNA |
DE10113815A1 (en) * | 2001-03-21 | 2002-10-02 | Eppendorf Ag | Process for the isolation of plasmids or proteins from suspended bacterial or yeast cells |
US20030211970A1 (en) * | 2001-06-01 | 2003-11-13 | Samuel Nochumson | Processing of plasmid-containing fluids |
US7208271B2 (en) | 2001-11-28 | 2007-04-24 | Applera Corporation | Compositions and methods of selective nucleic acid isolation |
JP2005538717A (en) | 2002-09-13 | 2005-12-22 | ヴァレンティス,インコーポレイテッド | Apparatus and method for preparative scale purification of nucleic acids |
US20040211724A1 (en) * | 2002-10-01 | 2004-10-28 | Gibson Gary L. | Method of separating components in a sample using silane-treated silica filter media |
US7264728B2 (en) | 2002-10-01 | 2007-09-04 | Dow Corning Corporation | Method of separating components in a sample using silane-treated silica filter media |
US8501402B2 (en) | 2003-03-24 | 2013-08-06 | Boehringer Ingelheim Rcv Gmbh & Co Kg | Methods and devices for producing biomolecules |
EP2527456B1 (en) | 2004-10-22 | 2018-05-16 | Revivicor Inc. | Transgenic porcines lacking endogenous immunoglobulin light chain |
KR100622606B1 (en) | 2005-06-16 | 2006-09-11 | (주)엘피스바이오텍 | Composition for the purification of plasmid dna by one step and rapid purification method thereof |
EP2088196A1 (en) | 2008-02-08 | 2009-08-12 | Boehringer Ingelheim RCV GmbH & Co KG | Methods and devices for producing biomolecules |
WO2010051288A1 (en) | 2008-10-27 | 2010-05-06 | Revivicor, Inc. | Immunocompromised ungulates |
KR101569832B1 (en) * | 2008-11-19 | 2015-11-18 | 삼성전자주식회사 | Method for separating genomic DNA and plasmid DNA from each other and kit for same |
JP6437250B2 (en) | 2014-09-03 | 2018-12-12 | 株式会社ミズホメディー | Pretreatment method and nucleic acid extraction kit used therefor |
CN116355893B (en) * | 2023-01-06 | 2024-02-20 | 广州国家实验室 | Magnetic bead method nucleic acid extraction kit and application thereof |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0389063A2 (en) * | 1989-03-23 | 1990-09-26 | Akzo Nobel N.V. | Process for isolating nucleic acid |
WO1992007863A1 (en) * | 1990-10-26 | 1992-05-14 | Diagen Institut Für Molekularbiologische Diagnostik Gmbh | Device and process for insulating nucleic acids from cell suspensions |
WO1993011218A1 (en) * | 1991-12-02 | 1993-06-10 | Qiagen Gmbh | Method and device for the isolation of cell components, such as nucleic acids, from natural sources |
WO1995002049A1 (en) * | 1993-07-09 | 1995-01-19 | Cambridge Molecular Technologies Limited | Purification method and apparatus |
EP0648776A1 (en) * | 1993-09-27 | 1995-04-19 | Becton, Dickinson and Company | Surface materials useful for DNA purification by solid phase extraction |
WO1995021849A1 (en) * | 1994-02-11 | 1995-08-17 | Qiagen Gmbh | Process for separating double-stranded/single-stranded nucleic acid structures |
JPH07250681A (en) * | 1994-03-15 | 1995-10-03 | Tomy Seiko:Kk | Method for extracting and purifying dna and device therefor |
DE4422044A1 (en) * | 1994-06-14 | 1995-12-21 | Invitek Gmbh | Simple isolation and purificn. of nucleic acid from various samples |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3639949A1 (en) * | 1986-11-22 | 1988-06-09 | Diagen Inst Molekularbio | METHOD FOR SEPARATING LONG CHAIN NUCLEIC ACIDS |
US5652141A (en) * | 1990-10-26 | 1997-07-29 | Oiagen Gmbh | Device and process for isolating nucleic acids from cell suspension |
JPH04360686A (en) * | 1991-06-04 | 1992-12-14 | Tosoh Corp | Purification of dna |
US5438128A (en) * | 1992-02-07 | 1995-08-01 | Millipore Corporation | Method for rapid purifiction of nucleic acids using layered ion-exchange membranes |
-
1997
- 1997-06-14 DE DE69719409T patent/DE69719409T2/en not_active Expired - Fee Related
- 1997-06-14 EP EP97109708A patent/EP0814156B1/en not_active Expired - Lifetime
- 1997-06-17 CA CA002207852A patent/CA2207852A1/en not_active Abandoned
- 1997-06-18 US US08/877,874 patent/US5916775A/en not_active Expired - Fee Related
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0389063A2 (en) * | 1989-03-23 | 1990-09-26 | Akzo Nobel N.V. | Process for isolating nucleic acid |
WO1992007863A1 (en) * | 1990-10-26 | 1992-05-14 | Diagen Institut Für Molekularbiologische Diagnostik Gmbh | Device and process for insulating nucleic acids from cell suspensions |
WO1993011218A1 (en) * | 1991-12-02 | 1993-06-10 | Qiagen Gmbh | Method and device for the isolation of cell components, such as nucleic acids, from natural sources |
WO1995002049A1 (en) * | 1993-07-09 | 1995-01-19 | Cambridge Molecular Technologies Limited | Purification method and apparatus |
EP0648776A1 (en) * | 1993-09-27 | 1995-04-19 | Becton, Dickinson and Company | Surface materials useful for DNA purification by solid phase extraction |
WO1995021849A1 (en) * | 1994-02-11 | 1995-08-17 | Qiagen Gmbh | Process for separating double-stranded/single-stranded nucleic acid structures |
JPH07250681A (en) * | 1994-03-15 | 1995-10-03 | Tomy Seiko:Kk | Method for extracting and purifying dna and device therefor |
DE4422044A1 (en) * | 1994-06-14 | 1995-12-21 | Invitek Gmbh | Simple isolation and purificn. of nucleic acid from various samples |
Non-Patent Citations (2)
Title |
---|
J.SAMBROOK, E.F. FRITSCH, T. MANIATIS: "Molecular Cloning. A laboratory manual. Vol. 1. Second edition", 1989, COLD SPRING HARBOR LABORATORY PRESS, COLD SPRING HARBOR, US, XP002055354 * |
PATENT ABSTRACTS OF JAPAN vol. 096, no. 002 29 February 1996 (1996-02-29) * |
Also Published As
Publication number | Publication date |
---|---|
US5916775A (en) | 1999-06-29 |
CA2207852A1 (en) | 1997-12-18 |
EP0814156B1 (en) | 2003-03-05 |
DE69719409T2 (en) | 2004-01-15 |
EP0814156A2 (en) | 1997-12-29 |
DE69719409D1 (en) | 2003-04-10 |
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