EP0748338A4 - In vitro reifung von antikörpern mittels alanin 'scanning' mutagenese - Google Patents
In vitro reifung von antikörpern mittels alanin 'scanning' mutageneseInfo
- Publication number
- EP0748338A4 EP0748338A4 EP95911994A EP95911994A EP0748338A4 EP 0748338 A4 EP0748338 A4 EP 0748338A4 EP 95911994 A EP95911994 A EP 95911994A EP 95911994 A EP95911994 A EP 95911994A EP 0748338 A4 EP0748338 A4 EP 0748338A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- affinity maturation
- alanine scanning
- scanning mutagenesis
- antibody affinity
- vitro antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 230000009824 affinity maturation Effects 0.000 title 1
- 238000012867 alanine scanning Methods 0.000 title 1
- 238000000338 in vitro Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
- C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
- C07K16/1036—Retroviridae, e.g. leukemia viruses
- C07K16/1045—Lentiviridae, e.g. HIV, FIV, SIV
- C07K16/1063—Lentiviridae, e.g. HIV, FIV, SIV env, e.g. gp41, gp110/120, gp160, V3, PND, CD4 binding site
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Virology (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Oncology (AREA)
- Hematology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- AIDS & HIV (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US20607994A | 1994-03-04 | 1994-03-04 | |
US206079 | 1994-03-04 | ||
PCT/US1995/002492 WO1995023813A1 (en) | 1994-03-04 | 1995-02-27 | In vitro antibody affinity maturation using alanine scanning mutagenesis |
Publications (2)
Publication Number | Publication Date |
---|---|
EP0748338A1 EP0748338A1 (de) | 1996-12-18 |
EP0748338A4 true EP0748338A4 (de) | 2001-03-28 |
Family
ID=22764890
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP95911994A Withdrawn EP0748338A4 (de) | 1994-03-04 | 1995-02-27 | In vitro reifung von antikörpern mittels alanin 'scanning' mutagenese |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP0748338A4 (de) |
JP (1) | JPH09509835A (de) |
CA (1) | CA2183550A1 (de) |
WO (1) | WO1995023813A1 (de) |
Families Citing this family (42)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6090382A (en) | 1996-02-09 | 2000-07-18 | Basf Aktiengesellschaft | Human antibodies that bind human TNFα |
DK0929578T3 (da) | 1996-02-09 | 2003-08-25 | Abbott Lab Bermuda Ltd | Humane antistoffer, der binder human TNFalfa |
US6037454A (en) | 1996-11-27 | 2000-03-14 | Genentech, Inc. | Humanized anti-CD11a antibodies |
US6025158A (en) * | 1997-02-21 | 2000-02-15 | Genentech, Inc. | Nucleic acids encoding humanized anti-IL-8 monoclonal antibodies |
US6133426A (en) * | 1997-02-21 | 2000-10-17 | Genentech, Inc. | Humanized anti-IL-8 monoclonal antibodies |
WO1998037200A2 (en) | 1997-02-21 | 1998-08-27 | Genentech, Inc. | Antibody fragment-polymer conjugates and humanized anti-il-8 monoclonal antibodies |
US7122636B1 (en) | 1997-02-21 | 2006-10-17 | Genentech, Inc. | Antibody fragment-polymer conjugates and uses of same |
DE19728697C1 (de) * | 1997-07-04 | 1999-03-25 | Deutsches Krebsforsch | Humaner Antikörper gegen ein Fusions(poly)peptid bzw. -protein, das einen Anteil von mindestens sechs Histidinen aufweist |
US7005504B2 (en) | 1998-01-22 | 2006-02-28 | Genentech, Inc. | Antibody fragment-peg conjugates |
US6468532B1 (en) | 1998-01-22 | 2002-10-22 | Genentech, Inc. | Methods of treating inflammatory diseases with anti-IL-8 antibody fragment-polymer conjugates |
US6458355B1 (en) | 1998-01-22 | 2002-10-01 | Genentech, Inc. | Methods of treating inflammatory disease with anti-IL-8 antibody fragment-polymer conjugates |
CA2868614A1 (en) | 2001-06-08 | 2002-12-08 | Abbott Laboratories (Bermuda) Ltd. | Methods of administering anti-tnf.alpha. antibodies |
US7647184B2 (en) | 2001-08-27 | 2010-01-12 | Hanall Pharmaceuticals, Co. Ltd | High throughput directed evolution by rational mutagenesis |
DE60332358D1 (de) | 2002-09-09 | 2010-06-10 | Hanall Pharmaceutical Co Ltd | Protease-resistente modifizierte interferon alpha polypeptide |
MY150740A (en) | 2002-10-24 | 2014-02-28 | Abbvie Biotechnology Ltd | Low dose methods for treating disorders in which tnf? activity is detrimental |
AU2004254352A1 (en) * | 2003-06-27 | 2005-01-13 | Roberto Crea | Look-through mutagenesis |
TW201705980A (zh) | 2004-04-09 | 2017-02-16 | 艾伯維生物技術有限責任公司 | 用於治療TNFα相關失調症之多重可變劑量療法 |
CA2903138A1 (en) | 2005-05-16 | 2006-11-23 | Abbvie Biotechnology Ltd. | Use of tnfa inhibitor for treatment of erosive polyarthritis |
SG170837A1 (en) | 2006-04-05 | 2011-05-30 | Abbott Biotech Ltd | Antibody purification |
US9399061B2 (en) | 2006-04-10 | 2016-07-26 | Abbvie Biotechnology Ltd | Methods for determining efficacy of TNF-α inhibitors for treatment of rheumatoid arthritis |
US9605064B2 (en) | 2006-04-10 | 2017-03-28 | Abbvie Biotechnology Ltd | Methods and compositions for treatment of skin disorders |
EP2007426A4 (de) | 2006-04-10 | 2010-06-16 | Abbott Biotech Ltd | Anwendungen und zusammensetzungen zur behandlung von psoriasis-arthritis |
WO2008154543A2 (en) | 2007-06-11 | 2008-12-18 | Abbott Biotechnology Ltd. | Methods for treating juvenile idiopathic arthritis |
NZ586828A (en) | 2008-01-15 | 2012-12-21 | Abbott Gmbh & Co Kg | Powdered antibody compositions and methods of making same |
EP2588498B1 (de) | 2010-06-29 | 2018-07-11 | Centre National de la Recherche Scientifique (CNRS) | Llt-1-antikörper mit neuen funktionellen eigenschaften |
EA201370076A1 (ru) * | 2010-09-22 | 2013-08-30 | Амген Инк. | Иммуноглобулины-переносчики и их применение |
EP2702077A2 (de) | 2011-04-27 | 2014-03-05 | AbbVie Inc. | Verfahren zur steuerung des galactosylierungsprofil von rekombinant exprimierten proteinen |
US9181572B2 (en) | 2012-04-20 | 2015-11-10 | Abbvie, Inc. | Methods to modulate lysine variant distribution |
WO2013158279A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Protein purification methods to reduce acidic species |
US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
WO2013176754A1 (en) | 2012-05-24 | 2013-11-28 | Abbvie Inc. | Novel purification of antibodies using hydrophobic interaction chromatography |
AU2013309506A1 (en) | 2012-09-02 | 2015-03-12 | Abbvie Inc. | Methods to control protein heterogeneity |
US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
AU2013381687A1 (en) | 2013-03-12 | 2015-09-24 | Abbvie Inc. | Human antibodies that bind human TNF-alpha and methods of preparing the same |
US8921526B2 (en) | 2013-03-14 | 2014-12-30 | Abbvie, Inc. | Mutated anti-TNFα antibodies and methods of their use |
US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
WO2014151878A2 (en) | 2013-03-14 | 2014-09-25 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides |
EP3052640A2 (de) | 2013-10-04 | 2016-08-10 | AbbVie Inc. | Verwendung von metallionen zur modulation von proteinglykosylierungsprofilen rekombinanter proteine |
US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
US8946395B1 (en) | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
WO2015073884A2 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
-
1995
- 1995-02-27 EP EP95911994A patent/EP0748338A4/de not_active Withdrawn
- 1995-02-27 CA CA 2183550 patent/CA2183550A1/en not_active Abandoned
- 1995-02-27 WO PCT/US1995/002492 patent/WO1995023813A1/en not_active Application Discontinuation
- 1995-02-27 JP JP7522980A patent/JPH09509835A/ja active Pending
Non-Patent Citations (5)
Title |
---|
CRAIG M. LEWIS ET AL: "Use of alanine scanning mutagenesis to improve the affinity of an anti-gp120 (HIV)antibody", JOURNAL OF CELLULAR BIOCHEMISTRY, SUPPLEMENT - KEYSTONE SYMPOSIUM ON ANTIBODY ENGINEERING, vol. 32, 7 March 1994 (1994-03-07) - 13 March 1994 (1994-03-13), XP000943396 * |
LISA K. DENZIN ET AL.: "Mutational analysis of active site contact residues in anti-fluorescein monoclonal antibody", MOLECULAR IMMUNOLOGY, vol. 30, no. 15, 1993, pages 1331 - 1345, XP000943436 * |
MIROSLAW K. GORNY ET AL.: "Human anti-V2 monoclonal antibody that neutralizes primary but not laboratory isolates of human immunodeficiency virus type I", JOURNAL OF VIROLOGY, vol. 68, no. 12, 15 December 1994 (1994-12-15), pages 8312-8320, XP000943394 * |
ROBERT F. KELLEY AND MARK P. O'CONNELL: "Thermodynamic analysis of an antibody functional epitope", BIOCHEMISTRY, vol. 32, 1993, pages 6828 - 6835, XP000943393 * |
See also references of WO9523813A1 * |
Also Published As
Publication number | Publication date |
---|---|
JPH09509835A (ja) | 1997-10-07 |
EP0748338A1 (de) | 1996-12-18 |
CA2183550A1 (en) | 1995-09-08 |
WO1995023813A1 (en) | 1995-09-08 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 19961004 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LI LU NL PT SE |
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A4 | Supplementary search report drawn up and despatched |
Effective date: 20010212 |
|
AK | Designated contracting states |
Kind code of ref document: A4 Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LI LU NL PT SE |
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STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 20020901 |