EP0491793B1 - 2'-desoxy-4'-thioribonucleosides utiles comme agents antiviraux et anticancereux - Google Patents

2'-desoxy-4'-thioribonucleosides utiles comme agents antiviraux et anticancereux Download PDF

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EP0491793B1
EP0491793B1 EP90913760A EP90913760A EP0491793B1 EP 0491793 B1 EP0491793 B1 EP 0491793B1 EP 90913760 A EP90913760 A EP 90913760A EP 90913760 A EP90913760 A EP 90913760A EP 0491793 B1 EP0491793 B1 EP 0491793B1
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Prior art keywords
deoxy
toluoyl
thio
uracil
compound
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EP0491793A1 (fr
EP0491793A4 (en
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John A. Montgomery
John A. Secrist, Iii
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Southern Research Institute
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/20—Antivirals for DNA viruses
    • A61P31/22—Antivirals for DNA viruses for herpes viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals

Definitions

  • This invention relates to ⁇ -2'-deoxy-4'-thioribonucleosides and intermediates useful in their production, and to the use of ⁇ -2'-de oxy-4'-thioribonucleosides as antiviral and anticancer agents.
  • a nucleoside is a molecule comprised of a pentose sugar in a furanose ring joined to a nitrogenous heterocyclic base that is a derivative of either purine or pyrimidine.
  • a 4'-thionucleoside is a nucleoside wherein the furanose ring oxygen has been replaced by sulfur.
  • a 2'-deoxy-4'-thioribonucleoside is a 4'-thionucleoside wherein the pentose sugar is 2'-deoxy-D-ribose.
  • nucleoside shall also include compounds wherein the nitrogenous heterocyclic base is a base related to the pyrimidine base, but with a ring alteration, such nitrogenous heterocyclic bases including 5-azapyrimidines, 6-azapyrimidines, and 3-deazapyrimidines and shall also include compounds having acyl protecting groups at the 3' position, or the 5' position, or both, of the 2'-deoxy-D-ribose.
  • Chem., 56, 794 (1977) disclose the synthesis of 9-(3-deoxy-4-thio- ⁇ -D-erythro-pentofuranosyl)adenine (4'-thiocordycepin).
  • Reist, et al, J. Org. Chem., 33, 189 (1968) describe the synthesis of adenine nucleosides of 4-thio-D-xylose and 4-thio-D-arabinose.
  • Ototani et al. J. Med.
  • Chem., 17, 535 (1974) disclose the preparation and antitumor activity of 4' -thio-1- ⁇ -D-arabinofuranosylcytosine and 2,2'-anhydro-4'-thio-1- ⁇ -D-arabinofuranosylcytosine hydrochloride.
  • EP 0 409 575 discloses anit-viral pyrimidine nucleosides wherein the sugar moiety is the 2-deoxy-4-thio-D-ribofuranose moiety.
  • the general formulae disclosed in EP 0 409 575 comprises a multiplicity of compounds, inter alia a 5-methyl-uracil.
  • a therapeutically effective amount of a 2'-deoxy-4'-thioribonucleoside as previously defined is used to be administered to a host animal, including man, afflicted with a viral infection, e.g., an infection caused by herpes simplex virus types 1 or 2.
  • a therapeutically effective amount of a 2'-deoxy-4'-thioribonucleoside as previously defined is used to be administered to a host animal, including man, afflicted with cancer.
  • cancer is meant any new and abnormal cell growth, specifically a new growth of tissue which is uncontrolled and progressive.
  • Compounds of this invention may be used, for example, in the treatment of leukemias, epidermoid carcinoma, lymphomas, choriocarcinoma, Wilm's tumor, neuroblastoma, rhabdomyosarcoma, carcinoma of the testis, and breast and lung tumors.
  • novel intermediates useful in the preparation of certain 2'-deoxy-4,'-thioribonucleosides are provided.
  • the 2'-deoxy-4'-thioribonucleosides are prepared by coupling compound 3 with nitrogenous heterocyclic bases and then removing the toluoyl protecting groups.
  • Compound 3 is coupled with a pyrimidine using the catalysts hexamethyldisilazane, trimethylchlorosilane, and trimethylsilyl trifluoromethanesulfonate (see Vorbruggen, et al, Chem. Ber., 114, 1234 (1981)).
  • Compound 3 is coupled with a 5-azapyrimidine, 6-azapyrimidine and 3-deazapyrimidine in a similar manner.
  • the coupling reaction provides both ⁇ and ⁇ nucleosides in most cases. Anomers may be separated by conventional methods.
  • the compound of formula 3 can be combined with uracil to give the compound of formula 15
  • the compound of formula 3 can also be combined with thymine to give the compound of formula 17
  • acyl protecting groups besides the toluoyl protecting group may be used.
  • conventional acyl protecting groups may be substituted.or added, using conventional methods, to the 3' position or 5' position, or both, of the 2'-deoxy-4'-thioribonucleosides.
  • MeOH is methyl alcohol
  • EtOH is ethyl alcohol
  • Me 2 SO-d 6 is deuterated dimethyl sulfoxide (CD 3 ) 2 SO.
  • reaction mixture was poured into an ice-water mixture, stirred for 1 hour, and then diluted with 500 mL of CHCl 3 to give a total volume of 1000 mL.
  • the aqueous layer was extracted with CHCl 3 (2 x 100 mL).
  • the combined organic extracts were washed with dilute sulfuric acid (200 mL), aqueous saturated sodium bicarbonate (2 x 200 mL) and water until neutral, dried over MgSO 4 , and evaporated to dryness.
  • 1-(2-Deoxy-4-thio-3,5-di-O-toluoyl-D-ribofuranosyl)uracil (formula 15).
  • HMDS hexamethyldisilazane
  • TMSCl trimethylchlorosilane
  • 1-(2-Deoxy-4-thio-D-ribofuranosyl)uracil (formula 16 ).
  • a solution of 1-(2-deoxy-4-thio-3,5-di-O-toluoyl- ⁇ -D-ribofuranosyl) uracil (formula 15 ) (175 mg, 0.36 mmol) in anhydrous MeOH (30 mL) was stirred at room temperature with a freshly prepared solution of sodium methoxide (39 mg, 0.72 mmol) in MeOH (7.5 mL).
  • a TLC aliquot at 2.5 hours showed complete reaction.
  • the solution was rendered neutral with Dowex 50W-X8 (H + ) ion-exchange resin, the suspension filtered, and the resin was washed with MeOH.
  • the results of these tests against herpes simplex virus, Type 1 and Type 2 are summarized in Table 1.
  • the Virus Rating (VR) is a standard weighted measurement of antiviral activity which takes into account the degree of inhibition of virus-induced cytopathogenic effects (CPE) and the degree of cytotoxicity produced by the test compound, determined by a modification of the method of Ehrlich et al, Ann. N.Y. Acad. Sci. 130, 5-16 (1965) .
  • the CPE-inhibition assays were designed to test seven 0.5 log 10 concentrations of each compound, beginning with 320 ⁇ g/mL, against HSV in triplicate 24-hour Vero cell monolayers in 96-well tissue culture plates. To each of the replicate cell cultures were dispensed 0.1 mL of the test compound solution (or suspension) and 0.1 mL of HSV suspension (diluted in medium to yield 32 CCID 50 units per 0.1 mL). Cell controls, untreated virus-infected controls and drug cytotoxicity controls were included in each assay. The plates were incubated at 37°C in a humidified atmosphere containing 2% CO 2 until 100% CPE were observed in the untreated virus control cultures. The cell monolayers were examined microscopically for drug cytotoxicity and for CPE. which was graded on a scale of 0-4 (0-100% CPE).
  • the VR was calculated as 0.1 of the sum of the numerical differences between the recorded CPE grade of each test well and that of the corresponding virus control in the culture plate. Numerical differences between the scores of test wells containing a drug concentration which is partially cytotoxic and their corresponding virus controls were halved.
  • a greater value of VR indicates greater antiviral activity.
  • a compound with a VR of 1.0 or greater is considered to have significant antiviral activity with a high degree of reproducibility in confirmatory in vitro tests.
  • a compound with a VR of 0.5-0.9 is considered to have possible or marginal activity; a compound with a VR of less than 0.5 is considered to be inactive.
  • the MIC 50 (minimum inhibitory concentration, 50%) is the concentration of a test compound required for 50% inhibition of virus-induced cytopathogenic effect calculated by using a regression analysis program for semilog curve fitting.
  • MTC minimum toxic concentration
  • TI is the therapeutic index, calculated by dividing the minimum cytotoxic drug concentration (MTC) by the minimum inhibitory concentration, 50% (MIC 50 ). The results were compared with two commercial antiviral agents, acyclovir and 9- ⁇ -D-arabinofuranosyladenine (Ara-A).
  • 2'-Deoxy-4'-thioribonucleosides were tested for antitumor activity against leukemia L1210 ("L1210”) cells and human epidermoid carcinoma No. 2 (“H.Ep.-2”) cells.
  • Table 2 sets forth the results of cytotoxicity tests.
  • the IC 50 is the concentration required to decrease cellular proliferation by 50% as compared to untreated controls.
  • the cells were grown in suspension cultures and the number of cells present was determined at 24 and 48 hours.
  • the values shown in Table 2 are 48 hour values.
  • the IC 50 is the concentration required to reduce colony formation by 50% as compared to controls.
  • One hundred cells in 10 mL of medium were placed in prescription bottles, and after 10 days incubation, the medium was decanted and the colonies were stained and counted.
  • IC 50 value of less than 40 ⁇ g/mL indicates a compound of interest
  • IC 50 value of less than 1 indicates a compound that is extremely effective.
  • Table 2 all the compounds tested show an IC 50 value of less than 40 ⁇ g/mL with respect to either H.Ep.-2 or L1210 cells, or both.
  • One compound, 1-(2-Deoxy-4-thio- ⁇ -D-ribofuranosyl) thymine shows an IC 50 value of much less than 1 ⁇ g/mL.
  • IC 50 ( ⁇ g/mL) Compound H. Ep. -2 L1210 1- (2-Deoxy-4-thio- ⁇ -D-ribofuranosyl)thymine 0.075 0.025 1- (2-Deoxy-4-thio- ⁇ -D-ribofuranosyl)thymine 2.7 20

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biotechnology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Hematology (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Claims (12)

  1. β-2'-désoxy-thioribonucléoside représenté par la formule :
    Figure 00340001
       où B est une base pyrimidine, 5-azapyrimidine, 6-azapyrimidine ou 3-déazapyrimidine, et R1 et R2 peuvent être identiques ou différents et sont des hydrogènes ou des groupements acyle protecteurs,
       où le substitut sur C-5 de B est soit de l'hydrogène, soit un groupement méthyle,
       à condition que, lorsque B est une base pyrimidine, ce ne soit pas de l'uracile comportant un groupement méthyle fixé sur le carbone hétérocyclique C-5.
  2. β-2'-désoxy-thioribonucléoside représenté par la formule :
    Figure 00340002
       où B est l'une des bases hétérocycliques azotées suivantes :
    Figure 00340003
    Figure 00340004
    Figure 00350001
    Figure 00350002
       où X9, X10, X11, X12, X13, X14 sont chacun de l'hydrogène ou un groupement méthyle, et R1 et R2 peuvent être identiques ou différents et sont de l'hydrogène ou des groupements acyle protecteurs, à condition que ce ne soit pas de l'uracile comportant un groupement méthyle fixé sur le carbone hétérocyclique C5.
  3. β-2'-désoxy-thioribonucléoside selon la revendication 1, où B est une base pyrimidine.
  4. β-2'-désoxy-4'-thioribonucléoside représenté par la formule
    Figure 00350003
    où B est l'une des bases pyrimidine suivantes :
    Figure 00360001
       où X17 et X18 sont un hydrocarbure ou un groupement méthyle, et R1 et R2 peuvent être identiques ou différents et sont des hydrogènes ou des groupements acyle protecteurs, à condition que ce ne soit de l'uracile comportant un groupement méthyle fixé sur le carbone hétérocyclique C5.
  5. Ribonucléoside selon l'une quelconque des revendications précédentes, où chacun de R1 et de R2 est de l'hydrogène.
  6. 1-(2-désoxy-4-thio-β-D-ribofuranosyl) uracile.
  7. 1-(2-désoxy-4-thio-3,5-di-O-toluoyl-β-D-ribofuranosyl) uracile.
  8. 1-(2-désoxy-4-thio-3,5-di-O-toluoyl-β-D-ribofuranosyl) thymine.
  9. Préparation pharmaceutique comprenant une quantité thérapeutiquement efficace d'un composé selon l'une quelconque des revendications 1 à 6.
  10. Utilisation d'un composé selon l'une quelconque des revendications 1 à 6 pour la fabrication d'un médicament destiné au traitement d'un animal hôte présentant une infection virale.
  11. Utilisation selon la revendication 10, dans laquelle l'infection virale est une infection par le virus de l'herpès simplex.
  12. Utilisation d'un composé selon l'une quelconque des revendications 1 à 6 pour la fabrication d'un médicament destiné au traitement d'un animal atteint d'un cancer.
EP90913760A 1989-09-15 1990-09-14 2'-desoxy-4'-thioribonucleosides utiles comme agents antiviraux et anticancereux Expired - Lifetime EP0491793B1 (fr)

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US40804089A 1989-09-15 1989-09-15
US408040 1989-09-15
PCT/US1990/005252 WO1991004033A1 (fr) 1989-09-15 1990-09-14 2'-desoxy-4'-thioribonucleosides utiles comme agents antiviraux et anticancereux

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EP0491793A1 EP0491793A1 (fr) 1992-07-01
EP0491793A4 EP0491793A4 (en) 1993-03-03
EP0491793B1 true EP0491793B1 (fr) 2005-11-30

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EP (1) EP0491793B1 (fr)
JP (1) JP3207852B2 (fr)
AT (1) ATE311399T1 (fr)
AU (1) AU6401490A (fr)
DE (1) DE69034209T2 (fr)
ES (1) ES2253732T3 (fr)
WO (1) WO1991004033A1 (fr)

Families Citing this family (8)

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Publication number Priority date Publication date Assignee Title
US5034506A (en) * 1985-03-15 1991-07-23 Anti-Gene Development Group Uncharged morpholino-based polymers having achiral intersubunit linkages
IE902574A1 (en) * 1989-07-17 1991-02-27 Univ Birmingham Antiviral pyrimidine nucleosides
MX9303985A (es) * 1992-07-02 1994-02-28 Wellcome Found Nucleosidos terapeuticos.
WO1994001117A1 (fr) * 1992-07-02 1994-01-20 The Wellcome Foundation Limited Nucleosides therapeutiques
GB9218810D0 (en) * 1992-09-04 1992-10-21 Univ Birmingham Antiviral pyrimidine nucleosides
AU747012B2 (en) * 1998-07-23 2002-05-09 Southern Research Institute Preparation of thioarabinofuranosyl compounds and use thereof
WO2013133399A1 (fr) * 2012-03-09 2013-09-12 大鵬薬品工業株式会社 Agent endommageant l'adn
WO2013137427A1 (fr) * 2012-03-16 2013-09-19 大鵬薬品工業株式会社 Nouveau composé nucléosidique de type pyrimidine

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988008001A1 (fr) * 1987-04-16 1988-10-20 Aktiebolaget Astra Nucleosides et analogues de nucleosides, compositions et procedes pharmaceutiques permettant la preparation des composes
EP0316017A2 (fr) * 1987-11-12 1989-05-17 Bristol-Myers Squibb Company 2',3'-Didéoxy-2'-fluoronucléosides

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988008001A1 (fr) * 1987-04-16 1988-10-20 Aktiebolaget Astra Nucleosides et analogues de nucleosides, compositions et procedes pharmaceutiques permettant la preparation des composes
EP0316017A2 (fr) * 1987-11-12 1989-05-17 Bristol-Myers Squibb Company 2',3'-Didéoxy-2'-fluoronucléosides

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FULMER SHEALY Y. ET AL: "Carbocyclic analogs of 5-fluorouracil nucleosides", J.MED.CHEM., vol. 24, no. 9, 1981, pages 1083 - 1086 *
J.CARBOHYDRATES. NUCLEOSIDES NUCLEOTIDES, vol. 5, no. 3, 1978, pages 187 - 224 *

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JPH05500666A (ja) 1993-02-12
ES2253732T3 (es) 2006-06-01
EP0491793A1 (fr) 1992-07-01
DE69034209D1 (de) 2006-01-05
EP0491793A4 (en) 1993-03-03
ATE311399T1 (de) 2005-12-15
JP3207852B2 (ja) 2001-09-10
DE69034209T2 (de) 2006-08-10
WO1991004033A1 (fr) 1991-04-04
AU6401490A (en) 1991-04-18

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