EP0428486B1 - Polymyxinkonjugate - Google Patents
Polymyxinkonjugate Download PDFInfo
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- EP0428486B1 EP0428486B1 EP90810872A EP90810872A EP0428486B1 EP 0428486 B1 EP0428486 B1 EP 0428486B1 EP 90810872 A EP90810872 A EP 90810872A EP 90810872 A EP90810872 A EP 90810872A EP 0428486 B1 EP0428486 B1 EP 0428486B1
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- Prior art keywords
- polymyxin
- conjugate
- conjugate according
- carrier
- pmb
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- Expired - Lifetime
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
- A61K47/61—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule the organic macromolecular compound being a polysaccharide or a derivative thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Definitions
- the invention relates to novel polymyxin conjugates and to methods of preparing and using them.
- Endotoxins or lipopolysaccharides are structural molecules derived from the cell walls of the Gram-negative bacteria. When introduced into the bloodstream, they can interfere with the regulation of body temperature and cause fever. They also have a toxic effect, leading to cardiac, pulmonary and kidney failure. Endotoxin-related diseases are a leading cause of death among those patients in intensive care units.
- polymyxins especially of polymyxin B ( PMB ) to neutralize endotoxin, accomplished by binding to the lipid A region of the endotoxin molecule.
- Polymyxin B from Bacillus polymyxa (B. aerosporus) is a highly charged amphiphilic cyclic peptidolipid. It is also useful in combating various fungal infections, especially those arising in immunocompromised individuals.
- PMB has some properties which renders it less than an ideal antibiotic.
- the present invention relates to polymyxin-carrier conjugates which are soluble in water, e.g. polymyxin A-, B- or E-carrier conjugates, particularly PMB-carrier conjugates. They are indicated for use in neutralizing endotoxin. They are an improvement over the administration of native polymyxin because they are more effective and are less toxic.
- a conjugate is water-soluble when it has a solubility in water of at least 0.5 mg/ml at 25°C, preferably of at least 1 mg/ml at 25°C.
- Such conjugates are water-soluble and injectable, non-matrix, colloid, not cross-linked, non-macromolecular, and/or non-particulate.
- the carrier which can be conjugated to polymyxin is chosen from molecules which can form water-soluble conjugates and which are uniform, non-toxic, non-carcinogenic, non-irritating and non-immunogenic. It is normally a biopolymer.
- Such carriers include polysaccharides such as dextran or hydroxy ethyl starch ( HES ), proteins such as albumin, and polymers such as polyvinyl pyrrolidone, polyethylene glycol and polyvinyl alcohol. Dextran is the most preferred.
- the size of the biopolymer portion i.e. the carrier part of the conjugate may vary. Typically it will range from a molecular weight of 25,000 to 500,000, preferably from 50,000 to 300,000, more preferably from 79,000 to 200,000.
- the size of the biopolymer chosen can significantly contribute to the duration of the conjugate's effective time of circulation in the blood stream. Generally, the larger the biopolymer, the longer the conjugate will stay in the circulation. Thus the size of the biopolymer can be adjusted to result in a conjugate whose time of duration in the body corresponds to a predetermined time.
- the conjugates of the invention may be prepared in conventional manner.
- polymyxin B this has five ⁇ -amino groups which bind to the bacterial endotoxin. At least one ⁇ -amino group can be used to securely bind the PMB to the carrier, but all five sites cannot be used for this purpose, or the conjugate will lack endotoxin-neutralizing activity.
- Various chemical reactions can be used to conjugate polymyxin to these carriers.
- the number of PMB molecules bound per molecule of polymer can be influenced by varying the ratios of reactants during the coupling reaction.
- the conjugate will contain between 1 PMB : 15 biopolymer to 200 PMB : 1 biopolymer, more preferably the conjugate will have from 1-10 PMB : 1 biopolymer, and even more preferably from 1-3 PMB : 1 biopolymer on a molar basis.
- the conjugates with high PMB : biopolymer ratios seem to possess greater potency than conjugates with lower ratios.
- a polymyxin-carrier conjugate more specifically, a PMB-dextran conjugate
- carbamate linkage Method A
- PMB-dextran conjugates made in this fashion are found to retain the anti-endotoxin activity of native PMB, and in addition are found to be devoid of the acute neurotoxicity exhibited by native PMB.
- These conjugated forms also show a 2-5 fold improved therapeutic index by decreasing chronic toxicity over that of native PMB.
- a second method of making conjugated polymyxin dextran is through an amine bond (method B).
- PMB-dextran conjugates made in this manner retain the same general anti-endotoxin activity or are more active than native PMB.
- These conjugated forms are completely devoid of any acute neurotoxicity seen by native PMB and exhibit a 33-fold improvement of the therapeutic index by reducing chronic toxicity over that seen with native PMB.
- one PMB-dextran conjugate is devoid of any measurable toxicity at the doses tested and retains good anti-endotoxin activity, resulting in an 80-fold improvement in the therapeutic index.
- conjugates produced by this method and "rapidly purified” exhibit a 60- to 120-fold improvement in TI.
- Rapidly purified as used throughout the specification and claims means that the material is purified from unbound PMB using molecular sieving chromatography, de-salting gels, or Amicon R ultrafiltration, occurs for approximately 12-24 hours, preferably approximately 18 hours (as opposed to an extended dialysis time of 7-10 days).
- conjugates produced by these methods and rapidly purified comprise another and preferred aspect of this invention.
- the polymyxin-carrier conjugate obtained from either of the two methods outlined above is, however, difficult to purify.
- a 10-day extended dialysis was tried in order to separate native PMB from the conjugate.
- the protein level inside the dialysis bag reached an asymptote, and gel permeation column chromatography showed a single large molecular weight species, the material still contained unbound PMB.
- pressure dialysis Amicon R
- Conjugates purified in this manner were equipotent to PMB, but still retained 1/3 to 1/5 the toxicity of PMB. While not intending to be bound by theory, it appears that there is some chemical association between the "free" PMB and the conjugate which renders its separation difficult. Upon injection in the animal the "free" PMB dissociates itself and causes toxicity problems.
- Conjugate purified in this manner is referred to herein as "ultra-pure" polymyxin-conjugate and generally can be described as having less than 20 ⁇ g unconjugated polymyxin per 1 mg total protein, and preferably less than 10 ⁇ g unconjugated polymyxin per 1 mg total protein.
- a further aspect of the invention is thus a process for the preparation of a water-soluble polymyxin-dextran conjugate comprising
- Substantially free from unbound polymyxin is herewith defined as a ratio of conjugate to unbound polymyxin of at least 95 : 1, preferably of at least 99 : 1 on a molar basis.
- the lower alcohol is e.g. of 1 to 10 carbon atoms, it preferably is of 1 to 4 carbon atoms, e.g. n-butanol or isopropanol, more preferably methanol or ethanol.
- the polymyxin preferably is PMB.
- Ultra-pure conjugate has virtually no toxicity and as a result an LD100 cannot be established for it. At a dose of 100 mg/kg no toxicity is seen. At higher concentrations the solution is too viscous to inject. This is in contrast to native PMB which has an LD0 i.v. of 5 mg/kg and an LD100 i.v. of 9.5 mg/kg. Thus the Therapeutic Index of ultra-pure PMB conjugate is over 1000-fold higher than native PMB, due to decreased toxicity.
- a second advantage in using the conjugate rather than native polymyxin is that the conjugate has a longer duration of activity and thus can be used prophylactically.
- one aspect of the invention is the prevention of diseases caused by the presence of bacterial endotoxin by administering to a subject in need of such prophylactic treatment a prophylactically-effective amount of a water-soluble conjugate of polymyxin, preferably PMB and a carrier, particularly dextran, and a pharmaceutically acceptable further carrier or diluent as appropriate, e.g. an inert solution.
- a water-soluble conjugate of polymyxin preferably PMB and a carrier, particularly dextran, and a pharmaceutically acceptable further carrier or diluent as appropriate, e.g. an inert solution.
- the invention also concerns a pharmaceutical composition
- a pharmaceutical composition comprising a water-soluble polymyxin-carrier conjugate together with further pharmaceutically acceptable carriers or diluents as appropriate, e.g an inert solution.
- the invention also concerns a process for the preparation of a pharmaceutical composition
- a process for the preparation of a pharmaceutical composition comprising mixing a water-soluble polymyxin-carrier conjugate with further pharmaceutically acceptable carriers or diluents as appropriate, e.g. an inert solution.
- the invention is thus indicated for use in the treatment of diseases caused by the presence of bacterial endotoxin comprising administering to a subject in need of such treatment an endotoxin-neutralizing amount of a water-soluble conjugate of polymyxin, particularly PMB and a carrier, particularly dextran, and an inert solution.
- polymyxin is also effective im combating bacterial or fungal infections
- this invention is also indicated for use in treating bacterial or fungal infections comprising administering to a subject in need of such treatment a bacteriocidally- or fungicidally-effective amount of a water-soluble conjugate of polymyxin and a carrier, particularly dextran, and an inert solution.
- the polymyxin-carrier conjugate of the invention can be used in a manner consistent with the use of polymyxin itself, i.e. it can be used alone as an antibiotic for bacterial or fungal infections or combined with other bacteriocidal agents and/or anti-inflammatory agents. It may be administered, e.g. intramuscularly, intravenously, intrathecally, subconjunctivally or topically.
- formulations for intramuscular injections typically comprise an effective amount of PMB-conjugate in sterile water, physiological saline or approximately 1 % procane ⁇ HCl.
- Intravenous formulations typically comprise an effective amount of PMB-conjugate in 5 % dextrose and sterile water.
- Intrathecal formulations typically comprise an effective amount of PMB-conjugate in physiologic saline.
- an effective amount can be mixed with water or physiological saline, and optionally glycerine, and cupric sulfate for eye drops, or it may be made into an ointment or suspension.
- Creams, for topical applications, especially for burned areas, typically comprise an effective amount of polymyxin-conjugate in a base of inactive ingredients such as liquid petrolatum, propylene glycol, polyoxyethylene, polyoxypropylene and emulsifying wax.
- the amount of polymyxin-conjugate to be used is based on the amount of native polymyxin which would typically be prescribed for a particular patient (taking into account such factors as the condition being treated and age and weight of the patient) and the activity of the particular polymyxin-conjugate used. Often, a dosage reduction of up to 50 % compared to native polymyxin can be realized due to the effective increased activity of the polymyxin conjugate, its reduced toxicity and its increased duration of activity.
- 600-1000 mg of PMB either powdered or presolubilized in water
- the entire reaction mixture is then transferred to a 50,000 molecular weight cut-off dialysis tubing and dialyzed against 0.05 M pyrogen-free phosphate buffer for 6-10 days.
- the final dialyzed reaction mixture is measured for protein content by spectrophotometry at 208 nm absorbance or at 595 nm using the Bradford reagent (Bio Rad, Richmond, CA, USA).
- Free amino groups are determined using the ninhydrin reaction, with native PMB as a control. Analysis for nitrogen and carbon content is done using a CHN Elemental Analyzer.
- molar ratio is determined by dividing the concentration of dextran used in conjugation (23.7 ⁇ mol) by the final PMB-protein concentration in ⁇ mol (based on 208 nm analysis) after dialysis. Dextran is abbreviated “dx”.
- C:N ratio assumes 450 molecules of water per 2 g dextran.
- the “bonds/PMB” is an estimate of the number of bonds by which PMB is attached to dextran, based on the ninhydrin reaction.
- ND is "not determined”.
- the oxidized dextran is then mixed with 2 g PMB dissolved in 80-200 ml Na-bicarbonate or Na-borate buffer (pH 8.5-9.0) and after 60 minutes, 40 ml 0.05 - 25 % Na-borohydride either in a single treatment or in multiple treatments, each treatment lasting 30 minutes to 24 hours is added.
- This reaction proceeds for 30 minutes and then is dialyzed for 7-10 days at 8° against 0.05 M pyrogen-free Na-phosphate buffer.
- the final dialyzed reaction mixture is analyzed for protein content and free amino groups as described in Example 1.
- the molar ratio is determined by dividing the amount of dextran used in conjugation (6.25 ⁇ mol) by the ⁇ mol final PMB protein (based on the 208 nm data) after dialysis. The percent nitrogen is determined using a CHN elemental analyzer.
- Bonds/PMB is estimated by the ninhydrin reaction: Summary of conjugation reactions Reaction NaIO4 (g) molar ratio % nitrogen bonds/PMB 1/50 0.014 1 dx : 3.64 PMB 0.21 3.6 1/10 0.071 1 dx : 12.9 PMB 1.05 2.9 1/5 0.142 1 dx : 26.8 PMB 1.71 2.6 1/4 0.178 1 dx : 29.8 PMB 2.76 3.0 1/21 0.355 1 dx : 39.6 PMB 3.74 2.2 stock* 0.710 1 dx :151 PMB 6.91 4.0 *Reaction mixture is turbid and remains so after dialysis 1Purified using rapid dialysis
- mice Male CB57BL/c mice (18-22 g, Jackson Labs) are used throughout this study. Animals are given endotoxin (0111B4 from List Biologicals, Palo Alto, CA, USA) and galactosamine to hypersensitize, as described in Galanos et al., Proc. Natl. Acad. Sci. USA 76 (1979) 5939. The endotoxin and galactosamine (320 mg/kg) are injected intraperitoneally in 0.5 ml pyrogen-free isotonic saline per animal between 13:00 and 15:00 to avoid diurnal variation. From a dose-response study, it is determined that 0.01 mg/kg endotoxin produces 85-95 % lethality. Animals are observed each day for 6 days after injection.
- PMB neuropeptide
- Male CB57BL/c mice (18-22 g, Jackson Labs) are used throughout this study. Animals are given either native PMB or conjugated PMB by intraperitoneal injection (0.5 ml/animal) and lethality is monitored for 7 days.
- the LD100 is determined by varying the dose of PMB (native and conjugated forms) that result in 100 % lethality. From 10 to 20 animals are used for each dose.
- PMB neuropeptide
- native and conjugated forms are evaluated for their ability to neutralize endotoxin by either pre-mixing with endotoxin for 60 minutes before i.p. injection or by coadministration of the substances as a single i.p. injection. While the concentration of the PMB (native and conjugated forms) are varied, the volume of each injection is kept constant at 0.5 ml per animal. The Protection by the conjugated PMB is compared to the native PMB (in terms of mg protein/kg). Controls (endotoxin, galactosamine and vehicle) are included as well in each study. Each group has between 10 and 17 animals.
- the Therapeutic Index (TI) for PMB is determined by dividing the LD100 by the PD100.
- the "treatment” refers to the conjugates appearing in Examples 1 and 2, supra .
- Anti-endotoxin data Treatment LD100 (mg/kg) PD100 (mg/kg) TI Native PMB1 25 2 12.5 Native PMB2 25 2 12.5 A >30 1 >30 B >28 >2 >14 C ND 5 ND D 140 2 70 1/5* 83 0.2 415 1/21* about 40-60 0.05 800-1200 stock2 >212 0.2 >1060 stock3 >212 20 >10.6 1Rapidly-purified material 2The PMB or conjugate is allowed to react with the endotoxin for 60 min before i.p.
- injection 3 The PMB or conjugate is injected simultaneously with the endotoxin *The fractions designate reaction conditions based on the amount of sodium periodate used in the conjugation reaction. If 710 mg is multiplied by the fraction the amount (in mg) of sodium periodate used in each sample is obtained.
- PMB-dextran conjugate is prepared essentially as described in Example 2, supra , to obtain conjugate designated "RXN 1/50".
- the conjugate a white flocculant
- 60 % methanol collected by centrifugation, and then subjected to ultrasonic resolubilization. This process is repeated seven times and the resulting material is evaluated for purity by gel permeation chromatography and reverse phase high pressure liquid chromatography (RP-HPLC). Although no free PMB is detected by gel permeation chromatography, RP-HPLC shows 48 ⁇ g unconjugated PMB per mg of total protein.
- the conjugate is then subjected to 3 additional precipitations to remove the unconjugated PMB.
- the resulting ultra-pure PMB-dextran conjugate is compared to native PMB in an endotoxin-induced lethality test as described in Example 3, C., supra .
- the ultra-pure PMB-conjugate gives > 70 % protection against a LD90 endotoxin challenge for at least 6 hours.
- this conjugate provides therapeutic protection 1.5 hours after administration of the endotoxin. Therefore the PMB-conjugate is suitable for prophylactic use.
- the ultra-pure PMB-conjugate has a surprisingly reduced toxicity as compared with native PMB.
- Native PMB has an LD0 i.v. of 5 mg/kg and an LD100 i.v. of 9.5 mg/kg, suggesting an early onset of toxicity and narrow therapeutic range.
- the ultra-pure PMB-conjugate has an LD0 of > 100 mg/kg and it has not been possible to establish an LD100 due to its inherent non-toxicity.
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Claims (32)
- Wasserlösliches Konjugat eines Polymyxins und eines Trägers, wobei das Konjugat eine Größe aufweist, die für die Verwendung im systemischen Kreislauf geeignet ist, mit der Maßgabe, daß, wenn das Polymyxin Polymyxin E ist, der Träger dann kein Proteinträger ist.
- Konjugat nach Anspruch 1, worin der Träger ein Polysaccharid ist, wie Dextran oder Hydroxyethylstärke, oder ein Polymer, wie Polyvinylpyrrolidon, Polyethylenglycol oder Polyvinylalkohol, wobei, wenn das Polymyxin ein anderes als Polymyxin E ist, der Träger auch ein Proteinträger, wie Albumin, sein kann.
- Konjugat nach Anspruch 1, worin das Polymyxin Polymyxin B (PMB) ist.
- Konjugat nach Anspruch 1, worin das Molverhältnis von Polymyxin B (PMB) zum Träger 1:15 bis 200:1 ist.
- Konjugat nach Anspruch 1, worin das Molverhältnis von Polymyxin B (PMB) zum Träger 1:1 bis 10:1 ist.
- Konjugat nach Anspruch 1, worin das Molverhältnis von Polymyxin B (PMB) zum Träger 1:1 bis 3:1 ist.
- Konjugat nach Anspruch 1, das eine Löslichkeit in Wasser von mindestens 1 mg/ml bei 25°C aufweist.
- Konjugat nach Anspruch 1, das injizierbar ist.
- Konjugat nach Anspruch 1, das nicht matrixartig ist.
- Konjugat nach Anspruch 1, das kolloidal ist.
- Konjugat nach Anspruch 1, das nicht quervernetzt ist.
- Konjugat nach Anspruch 1, das nicht makromolekular ist.
- Konjugat nach Anspruch 1, das nicht partikulär ist.
- Konjugat nach Anspruch 1, worin der Träger einheitlich, nicht toxisch, nicht carcinogen, nicht reizend und nicht immunogen ist.
- Konjugat nach Anspruch 1, worin der Träger ein Molekulargewicht von 25 000 bis 500 000 aufweist.
- Konjugat nach Anspruch 1, worin der Träger ein Molekulargewicht von 50 000 bis 300 000 aufweist.
- Konjugat nach Anspruch 1, worin der Träger ein Molekulargewicht von 79 000 bis 200 000 aufweist.
- Konjugat nach Anspruch 1, das Endotoxin-neutralisierende Aktivität aufweist.
- Konjugat nach Anspruch 1, das ein Molverhälnis von Konjugat zu ungebundenem Polymyxin von mindestens 99:1 aufweist.
- Konjugat nach Anspruch 2, worin der Träger Dextran ist.
- Konjugat nach Anspruch 3, worin der Träger Dextran ist.
- Konjugat nach Anspruch 21, worin Polymyxin B (PMB) durch eine Carbamatbindung gebunden ist.
- Konjugat nach Anspruch 21, worin Polymyxin B (PMB) durch Aminbindungen gebunden ist.
- Wasserlösliches Konjugat von Polymyxin B (PMB) und einem Träger mit Anti-Endotoxin Aktivität, wobei der Träger eine für die Verwendung im systemischen Kreislauf geeignete Größe hat und das Molverhältnis von Polymyxin B zum Träger 1:15 bis 200:1 beträgt.
- Konjugat nach Anspruch 24, wobei der Träger für die Angabe und Zirkulation in den Blutstrom geeignet ist.
- Konjugat nach einem der Ansprüche 1 bis 25 zur Verwendung als Pharmazeutikum.
- Konjugat nach Anspruch 26 zur Verwendung bei der Neutralisierung der Endotoxinwirkung.
- Verfahren zur Herstellung eines Polymyxin-Dextran-Konjugats nach Anspruch 20, gekennzeichnet durcha) geeignete Konjugation von Polymyxin an Dextran unter Bildung eines wasserlöslichen Polymyxin-Dextran-Konjugats einer Größe, die zur Verwendung im systemischen Kreislauf geeignet ist,b) Fällung des entstehenden Konjugats in einem niederen Alkohol,c) Resuspendierung des Konjugats, undd) Wiederholung der Schritte b) und c), bis das Konjugat im wesentlichen frei von ungebundenem Polymyxin ist.
- Pharmazeutische Zusammensetzung, die ein Konjugat nach einem der Ansprüche 1 bis 25 zusammen mit weiteren pharmazeutisch annehmbaren Trägern oder Verdünnungsmitteln enthält.
- Verwendnung eines Konjugats nach einem der Ansprüche 1 bis 25 zur Herstellung eines Arzneimittels zur prophylaktischen oder heilenden therapeutischen Anwendung als Endotoxin-neutralisierendes Mittel.
- Verwendung nach Anspruch 30 als antifungales Mittel.
- Verwendung nach Anspruch 30 als antibakterielles Mittel.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US43748789A | 1989-11-15 | 1989-11-15 | |
US437487 | 1989-11-15 |
Publications (2)
Publication Number | Publication Date |
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EP0428486A1 EP0428486A1 (de) | 1991-05-22 |
EP0428486B1 true EP0428486B1 (de) | 1994-09-21 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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EP90810872A Expired - Lifetime EP0428486B1 (de) | 1989-11-15 | 1990-11-13 | Polymyxinkonjugate |
Country Status (17)
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EP (1) | EP0428486B1 (de) |
JP (1) | JPH082917B2 (de) |
KR (1) | KR0184858B1 (de) |
AT (1) | ATE111746T1 (de) |
AU (1) | AU639952B2 (de) |
CA (1) | CA2029997A1 (de) |
CY (1) | CY2052B1 (de) |
DE (1) | DE69012747T2 (de) |
DK (1) | DK0428486T3 (de) |
ES (1) | ES2063326T3 (de) |
HK (1) | HK49696A (de) |
HU (1) | HU210901A9 (de) |
IE (1) | IE65268B1 (de) |
IL (1) | IL96326A (de) |
MY (1) | MY104520A (de) |
NZ (1) | NZ236050A (de) |
ZA (1) | ZA909187B (de) |
Families Citing this family (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6579696B1 (en) * | 1992-12-21 | 2003-06-17 | Promega Corporation | Polymyxin B conjugates |
WO1994014437A1 (en) * | 1992-12-21 | 1994-07-07 | Ophidian Pharmaceuticals, Inc. | Prevention and treatment of sepsis |
US6660267B1 (en) | 1992-12-21 | 2003-12-09 | Promega Corporation | Prevention and treatment of sepsis |
US5545721A (en) * | 1992-12-21 | 1996-08-13 | Ophidian Pharmaceuticals, Inc. | Conjugates for the prevention and treatment of sepsis |
US6180134B1 (en) | 1993-03-23 | 2001-01-30 | Sequus Pharmaceuticals, Inc. | Enhanced ciruclation effector composition and method |
DE122009000058I1 (de) * | 1993-09-22 | 2009-12-31 | Jackson H M Found Military Med | Verfahren zur aktivierung von löslichem kohlenhydraten durch verwendung von neuen cyanylierungsreagenzien, zur herstellung von immunogenischen konstrukten |
US5849301A (en) * | 1993-09-22 | 1998-12-15 | Henry M. Jackson Foundation For The Advancement Of Military Medicine | Producing immunogenic constructs using soluable carbohydrates activated via organic cyanylating reagents |
JP2581425B2 (ja) * | 1993-11-12 | 1997-02-12 | 株式会社島津製作所 | ペプチド合成用支持体及びそれを用いて製造されたペプチド |
AU712981B2 (en) * | 1995-03-22 | 1999-11-18 | Henry M. Jackson Foundation For The Advancement Of Military Medicine | Producing immunogenic constructs using soluble carbohydrates activated via organic cyanylating reagents |
KR100417183B1 (ko) * | 1995-03-22 | 2004-05-31 | 헨리 엠. 잭슨 파운데이션 포 더 어드벤스먼트 오브 밀리터리 메디신 | 유기시아닐화제로활성화된가용성탄수화물을이용한면역원성구조체제조 |
US5691304A (en) * | 1995-05-25 | 1997-11-25 | Novartis Ag | Improved process for preparing polymyxin B/dextran conjugates |
US6309646B1 (en) | 1996-05-09 | 2001-10-30 | The Henry M. Jackson Foundation For The Advancement Of Military Medicine | Protein-polysaccharide conjugate vaccines and other immunological reagents prepared using homobifunctional and heterobifunctional vinylsulfones, and processes for preparing the conjugates |
US5998381A (en) * | 1996-12-06 | 1999-12-07 | Ophidian Pharmaceuticals, Inc. | Compounds that bind bacterial pili |
IT1312110B1 (it) | 1999-05-18 | 2002-04-04 | Istituto Biochimico Pavese Pha | Addotti antibiotico-polimero polisaccaridico naturale |
DE10129369C1 (de) | 2001-06-21 | 2003-03-06 | Fresenius Kabi De Gmbh | Wasserlösliches, einen Aminozucker aufweisendes Antibiotikum in Form eines Pol ysaccharid-Konjugats |
AT507846B1 (de) * | 2009-01-22 | 2011-12-15 | Fresenius Medical Care De Gmbh | Sorptionsmittel für endotoxine |
RU2712276C2 (ru) * | 2014-10-03 | 2020-01-28 | Кселлия Фармасьютикалз Апс | Композиции |
CN109843315A (zh) * | 2016-10-21 | 2019-06-04 | 阿尔吉法玛公司 | 多粘菌素-藻酸盐低聚物缀合物 |
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SE7702261L (sv) * | 1976-03-02 | 1977-09-07 | Pharmaco Inc | Forfarande for att immobilisera antimikrobiella emnen |
JPS5813519A (ja) * | 1981-07-16 | 1983-01-26 | Seikagaku Kogyo Co Ltd | エンドトキシン吸着材及びそれを用いるエンドトキシンの除去方法 |
US4496689A (en) * | 1983-12-27 | 1985-01-29 | Miles Laboratories, Inc. | Covalently attached complex of alpha-1-proteinase inhibitor with a water soluble polymer |
-
1990
- 1990-11-13 DK DK90810872.3T patent/DK0428486T3/da active
- 1990-11-13 MY MYPI90002000A patent/MY104520A/en unknown
- 1990-11-13 IL IL9632690A patent/IL96326A/en unknown
- 1990-11-13 EP EP90810872A patent/EP0428486B1/de not_active Expired - Lifetime
- 1990-11-13 AT AT90810872T patent/ATE111746T1/de not_active IP Right Cessation
- 1990-11-13 ES ES90810872T patent/ES2063326T3/es not_active Expired - Lifetime
- 1990-11-13 JP JP2304230A patent/JPH082917B2/ja not_active Expired - Lifetime
- 1990-11-13 AU AU66555/90A patent/AU639952B2/en not_active Ceased
- 1990-11-13 DE DE69012747T patent/DE69012747T2/de not_active Expired - Fee Related
- 1990-11-13 NZ NZ236050A patent/NZ236050A/xx unknown
- 1990-11-14 IE IE410590A patent/IE65268B1/en not_active IP Right Cessation
- 1990-11-14 KR KR1019900018434A patent/KR0184858B1/ko not_active IP Right Cessation
- 1990-11-14 CA CA002029997A patent/CA2029997A1/en not_active Abandoned
- 1990-11-15 ZA ZA909187A patent/ZA909187B/xx unknown
-
1995
- 1995-06-02 HU HU95P/P00162P patent/HU210901A9/hu unknown
-
1996
- 1996-03-21 HK HK49696A patent/HK49696A/xx not_active IP Right Cessation
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1998
- 1998-04-30 CY CY9802052A patent/CY2052B1/xx unknown
Also Published As
Publication number | Publication date |
---|---|
ATE111746T1 (de) | 1994-10-15 |
DK0428486T3 (da) | 1995-01-09 |
KR910009274A (ko) | 1991-06-28 |
ZA909187B (en) | 1992-07-29 |
IE65268B1 (en) | 1995-10-18 |
DE69012747T2 (de) | 1995-03-16 |
MY104520A (en) | 1994-04-30 |
NZ236050A (en) | 1993-04-28 |
IL96326A (en) | 1995-07-31 |
AU639952B2 (en) | 1993-08-12 |
IL96326A0 (en) | 1991-08-16 |
CA2029997A1 (en) | 1991-05-16 |
JPH082917B2 (ja) | 1996-01-17 |
KR0184858B1 (ko) | 1999-05-01 |
AU6655590A (en) | 1991-05-23 |
EP0428486A1 (de) | 1991-05-22 |
HK49696A (en) | 1996-03-29 |
HU210901A9 (en) | 1995-09-28 |
IE904105A1 (en) | 1991-05-22 |
JPH03220198A (ja) | 1991-09-27 |
DE69012747D1 (de) | 1994-10-27 |
ES2063326T3 (es) | 1995-01-01 |
CY2052B1 (en) | 1998-04-30 |
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