EP0271154B1 - Enzymatic detergent composition - Google Patents
Enzymatic detergent composition Download PDFInfo
- Publication number
- EP0271154B1 EP0271154B1 EP87202386A EP87202386A EP0271154B1 EP 0271154 B1 EP0271154 B1 EP 0271154B1 EP 87202386 A EP87202386 A EP 87202386A EP 87202386 A EP87202386 A EP 87202386A EP 0271154 B1 EP0271154 B1 EP 0271154B1
- Authority
- EP
- European Patent Office
- Prior art keywords
- lipase
- protease
- pseudomonas
- lipases
- detergent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D7/00—Compositions of detergents based essentially on non-surface-active compounds
- C11D7/22—Organic compounds
- C11D7/40—Products in which the composition is not well defined
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
Definitions
- the present invention relates to an enzymatic detergent composition which comprises a special class of lipases and a special class of proteases.
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Detergent Compositions (AREA)
- Enzymes And Modification Thereof (AREA)
Description
- The present invention relates to an enzymatic detergent composition which comprises a special class of lipases and a special class of proteases.
- EP-A 0 130 064 (Novo) describes enzymatic detergent additives based on lipase from Fusarium ox- ysporum together with (in certain embodiments) a protease, for example Alcalase (Trade Mark). Although the fact is not mentioned in EP 0 130 064, Alcalase has a pl of less than 10.0.
- EP-A 0 214 761 (Novo) (published 18 March 1987) is concerned with enzymatic detergent additives based on lipase from Pseudomonas cepacia. In certain embodiments, Alcalase (Trade Mark) is also proposed as a constituent of the detergent additives of EP 0 214 761.
- In EP-A 0 206 390 we have described detergent compositions with a special class of lipases. In that patent application we have also described how these lipases rapidly lose activity in the presence of proteases in clean model systems, but that under practical wash conditions in washing machines a substantial benefit is still delivered by these lipases in the presence of proteases.
- We have now found that with the use of a particular class of proteases an improved overall performance is obtained with these lipase-containing detergent compositions, the lipolytic activity being substantially less affected by these proteases than by other proteases. This particular class of proteases consists of proteases having an isoelectric point of lower than 10.0, preferably lower than about 9. Such proteases are known in the art and typical examples thereof are Alcalase (ex Novo Industri), Maxatase (ex Gist Brocades), Optimase (ex Miles-Kali Chemie) and Kazusase (ex Showa Denka) (=API-21 = AP-1), Subtilising BPN' ex B. amvloliauefaciens (ATCC 23844).
- Kazusase is the preferred protease of the present invention; it has been described in the published Dutch patent application 8302790 of Showa Denka. Its isoelectric point is 7.4 according to this patent application. The isoelectric points of the other above-mentioned commercially available proteases all lie in the range of 8.7-9.4.
- Mixtures of proteases according to the present invention may also be used: In general, the amount of protease in the detergent composition will be from 0.1-50 GU/mg, usually 0.2-40 and preferably 0.5-30 GU/mg, based on the final detergent composition. A GU (glycine unit) is the amount of enzyme which under standard incubation conditions produces an amount of terminal NH2-groups equivalent to 1 micro- gramme/ml of glycine.
- The class of lipases used in the present invention embraces those lipases which show a positive immunological cross-reaction with the antibody of the lipase, produced by the microorganism Chromobacter viscosum var. lipolyticum NRRL B-3673. This lipase has been described in Dutch patent specification 154, 269 of Toyo Jozo KK, and the microorganism is available to the public at the United States Department of Agriculture, Agricultural Research Service, Northern utilization and Development Division, Peoria, Illinois under N° NRRL B-3673. This lipase will be referred to as the "Toyo Jozo " lipase.
- The lipases of the present invention should show a positive immunological cross-reaction with the Toyo Jozo lipase antibody, using the standard and well-known immunodiffusion procedure according to Oucherlony (Acta Med. Scan., 133, pages 76-79 (1950)).
- The preparation of the antiserum is carried out as follows:
- Equal volumes of 0.1 mg/ml antigen and of Freund's adjuvant (complete or incomplete) are mixed until an emulsion is obtained. Two female rabbits are injected with 2 ml samples of the emulsion according to the following scheme:
- day 0 : antigen in complete Freund's adjuvant
- day 4 : antigen in complete Freund's adjuvant
- day 32 : antigen in incomplete Freund's adjuvant
- day 60 : booster of antigen in incomplete Freund's adjuvant
- The serum containing the required antibody is prepared by centrifugation of clotted blood, taken on day 67.
- The titre of the anti-Toyo Jozo-lipase antiserum is determined by the inspection of precipitation of serial dilutions of antigen and antiserum according to the Ouchterlony procedure. A 25 dilution of antiserum was the dilution that still gave a visible precipitation with an antigen concentration of 0.1 mg/ml.
- All lipases showing a positive immunological cross-reaction with the Toyo Jozo-lipase antibody as hereabove described are lipases according to the present invention. Typical examples thereof are the lipase ex Pseudomonas fluorescens IAM 1057 (available under the trade name Amano-P lipase), the lipase ex Pseudomonas fragi FERM P 1339 (available under the trade name Amano-B), lipase ex Pseudomonas nitroreducens var. lipolyticum FERM P-1338, the lipase ex Pseudomonas sp., available under the trade name Amano-CES, lipases ex Pseudomonas cepacia, lipases ex Chromobacter viscosum, e.g. Chromobacter viscosum var. lipolyticum NRRL B-3673, commercially available from Toyo Jozo Co., Tagata, Japan; and further Chromobacter viscosum lipases from US Biochemical Corp., USA and Dio- synth Co., The Netherlands, and lipases ex Pseudomonas aladioli.
- The lipases of the present invention are included in the detergent and bleaching composition in such an amount that the final composition has a lipolytic enzyme activity of from 100 to 0.005 LU/mg, preferably 25 to 0.05 LU/mg of the composition.
- A Lipase Unit (LU) is that amount of lipase which produces 1/gmol of titratable fatty acid per minute in a pH stat. under the following conditions: temperature 30°C; pH = 9.0; substrate is an emulsion of 3.3 wt.% of olive oil and 3.3% gum arabic, in the presence of 13 mmol/l Ca2+ and 20 mmol/I NaCi in 5 mmol/I Tris- buffer.
- Naturally, mixtures of the above lipases can be used. The lipases can be used in their impurified form or in a purified form, e.g. purified with the aid of well-known adsorption methods, such as a phenyl sepha- rose-packed column technique.
- The detergent compositions of the present invention furthermore comprise one or more detergent surfactants, such as fatty acid soaps, synthetic anionic, nonionic, cationic, amphoteric and zwitterionic detergent surfactants. These detergent surfactants are well known in the art, and suitable examples are fully described in Schwartz, Perry and Berch, "Surface Active Agents and Detergents ", Vol.1 (1949) and Vol. II (1958) and in Schick, "Nonionic Surfactants ", Vol.1 (1967).
- In general, the composition contains from 1-50%, usually from 2-30% and preferably from 5-25% by weight of one or more detergent surfactants.
- The detergent compositions may furthermore include usual detergent ingredients in the usual amounts. They may be unbuilt or built, and may be of the zero-P type (i.e. not containing phosphorus-containing builders). Thus, the compositions may contain from 1-60%, preferably from 5-30% by weight of one or more organic and/or inorganic builders. Typical examples of such builders are the alkali metal ortho-, py- ro- and tri- polyphosphates, alkali metal carbonates, either alone or in admixture with calcite, alkali metal citrates, alkali metal nitrilotriacetates, carboxymethyloxy succinates, zeolites, polyacetal carboxylates and so on. Furthermore, they may contain from 1-35% of a bleaching agent or a bleaching system comprising a bleaching agent and an activator therefor, such as sodium perborate and tetraacetyl ethylene diamine.
- The compositions may furthermore comprise lather booster, foam depressors, anti-corrosion agents, soil-suspending agents, sequestering agents, anti-soil redeposition agents, perfumes, dyes, stabilising agents for the enzymes and bleaching agents and so on. They may also comprise enzymes other than the lipases and the proteases, such as amylases, oxidases and cellulases.
- The compositions of the present invention can be formulated in any desired form, such as powders, bars, pastes, liquids, etc.
- The invention will further be illustrated by way of Example.
- Washing experiments were carried out in a Tergotometer under the following conditions:
- washing time and temperature: 14 minutes at 40°C;
- three rinses with cold water
- detergent composition concentration: 1.2 g/l
- water hardness: 16°FH
- agitation: 100 rpm
- test cloth: cotton, soiled with AS 8 / groundnut oil / milk powder
- lipase: lipase ex Pseudomonas gladioli or lipase Amano-P or Cepacia lipase at 1 LU/ml
- protease: Alcalase at 20 GU/ml
-
- The reflectance of the test cloths was determined in a Reflectometer at 460 nm with a UV filter in the light pathway, and the residual percentage of fatty material on the test cloths was determined by extracting the dried cloths with petroleum ether, and determining the amount of fatty matter from the weight loss of the test cloth.
-
-
-
- 4° wash result of multi-cycle washing (MCSW).
- Soiling : Cotton soiled with mixture of inorganic pigments, palm oil (A) and protein (Cocktail I (B))
- Conditions: 5 g/I detergent components
- 30 min. at 30°C
- 40°FH
- protease : 20 GU/ml
- Cepacia lipase : 1 LU/ml
- 3.5 kg soiled load present; AS10 as single wash monitor for protease effects.
- N : N: Number of individual MCSW experiments
- Esperase HAP Y: pl >10
- Alcalase Kazusase: pl <10
-
- The performance of Cepacia lipase in the presence of alkaline and high alkaline proteases on test cloths in washing machines in the detergent composition of Example 3 was measured. (4° wash results of MCSW)
- - multi wash : cotton test cloths soiled with a mixture of inorganic pigments, groundnut oil, without (A) or with (B) protein (Cocktail I)
-
- - 30 min. at 30°C
- - 27°FH
- - protease : 20 GU/ml
- - Cepacia lipase : 1 LU/ml
- - 3.5 kg soiled load present
- Example 4 was repeated.
- Conditions - soiling: palm oil instead of groundnut
- - Amano-P lipase: 1 LU/ml
- - Gladioli lipase: 1 LU/ml
-
Claims (5)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB868629536A GB8629536D0 (en) | 1986-12-10 | 1986-12-10 | Enzymatic detergent composition |
GB8629536 | 1986-12-10 |
Publications (3)
Publication Number | Publication Date |
---|---|
EP0271154A2 EP0271154A2 (en) | 1988-06-15 |
EP0271154A3 EP0271154A3 (en) | 1988-08-03 |
EP0271154B1 true EP0271154B1 (en) | 1990-06-27 |
Family
ID=10608786
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP87202386A Expired - Lifetime EP0271154B1 (en) | 1986-12-10 | 1987-12-02 | Enzymatic detergent composition |
Country Status (11)
Country | Link |
---|---|
US (1) | US4824599A (en) |
EP (1) | EP0271154B1 (en) |
JP (1) | JPH0696718B2 (en) |
KR (1) | KR920004720B1 (en) |
AU (1) | AU607953B2 (en) |
BR (1) | BR8706683A (en) |
CA (1) | CA1288367C (en) |
DE (1) | DE3763423D1 (en) |
ES (1) | ES2016340B3 (en) |
GB (1) | GB8629536D0 (en) |
ZA (1) | ZA879298B (en) |
Families Citing this family (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0218272B1 (en) * | 1985-08-09 | 1992-03-18 | Gist-Brocades N.V. | Novel lipolytic enzymes and their use in detergent compositions |
GB8629535D0 (en) * | 1986-12-10 | 1987-01-21 | Unilever Plc | Enzymatic detergent composition |
BE1001436A3 (en) * | 1988-02-22 | 1989-10-31 | Synfina Sa | New lipase and detergent compositions containing. |
US4959179A (en) * | 1989-01-30 | 1990-09-25 | Lever Brothers Company | Stabilized enzymes liquid detergent composition containing lipase and protease |
US5089163A (en) * | 1989-01-30 | 1992-02-18 | Lever Brothers Company, Division Of Conopco, Inc. | Enzymatic liquid detergent composition |
US5665587A (en) * | 1989-06-26 | 1997-09-09 | Novo Nordisk A/S | Modified subtilisins and detergent compositions containing same |
DE69033633T2 (en) | 1989-07-07 | 2001-05-03 | Unilever Nv | Process for the production of a protein by means of a mushroom transformed by multi-copy integration of an expression vector |
US5658871A (en) * | 1989-07-07 | 1997-08-19 | Lever Brothers Company, Division Of Conopco, Inc. | Microbial lipase muteins and detergent compositions comprising same |
GB8921995D0 (en) * | 1989-09-29 | 1989-11-15 | Unilever Plc | Perfumed laundry detergents |
ES2174820T3 (en) * | 1991-01-16 | 2002-11-16 | Procter & Gamble | COMPOSITIONS OF COMPACT DETERGENTS WITH HIGH ACTIVITY CELL. |
US5883064A (en) * | 1993-12-21 | 1999-03-16 | The Procter & Gamble Company | Protease containing dye transfer inhibiting composition |
BE1008998A3 (en) * | 1994-10-14 | 1996-10-01 | Solvay | Lipase, microorganism producing the preparation process for the lipase and uses thereof. |
ES2144649T3 (en) * | 1994-11-18 | 2000-06-16 | Procter & Gamble | DETERGENT COMPOSITIONS CONTAINING LIPASE AND PROTEASE. |
EP2166076A1 (en) * | 2008-09-23 | 2010-03-24 | The Procter & Gamble Company | Cleaning composition |
WO2014200656A1 (en) | 2013-06-13 | 2014-12-18 | Danisco Us Inc. | Alpha-amylase from streptomyces umbrinus |
WO2014200657A1 (en) | 2013-06-13 | 2014-12-18 | Danisco Us Inc. | Alpha-amylase from streptomyces xiamenensis |
WO2014200658A1 (en) | 2013-06-13 | 2014-12-18 | Danisco Us Inc. | Alpha-amylase from promicromonospora vindobonensis |
WO2014204596A1 (en) | 2013-06-17 | 2014-12-24 | Danisco Us Inc. | Alpha-amylase from bacillaceae family member |
WO2015050724A1 (en) | 2013-10-03 | 2015-04-09 | Danisco Us Inc. | Alpha-amylases from a subset of exiguobacterium, and methods of use, thereof |
WO2015050723A1 (en) | 2013-10-03 | 2015-04-09 | Danisco Us Inc. | Alpha-amylases from exiguobacterium, and methods of use, thereof |
WO2015077126A1 (en) | 2013-11-20 | 2015-05-28 | Danisco Us Inc. | Variant alpha-amylases having reduced susceptibility to protease cleavage, and methods of use, thereof |
EP3034597A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Detergent composition |
EP3034588B1 (en) | 2014-12-17 | 2019-04-24 | The Procter and Gamble Company | Detergent composition |
EP3034596B2 (en) * | 2014-12-17 | 2021-11-10 | The Procter & Gamble Company | Detergent composition |
WO2017173324A2 (en) | 2016-04-01 | 2017-10-05 | Danisco Us Inc. | Alpha-amylases, compositions & methods |
WO2017173190A2 (en) | 2016-04-01 | 2017-10-05 | Danisco Us Inc. | Alpha-amylases, compositions & methods |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1619087A1 (en) * | 1967-08-14 | 1969-10-02 | Henkel & Cie Gmbh | Surfactant combinations which can be used as laundry detergents and detergents or auxiliary washing agents containing them |
GB1273545A (en) * | 1968-06-24 | 1972-05-10 | Albright & Wilson | Multi-enzyme cleaning compositions |
US4011169A (en) * | 1973-06-29 | 1977-03-08 | The Procter & Gamble Company | Stabilization and enhancement of enzymatic activity |
JPS6055118B2 (en) * | 1982-02-08 | 1985-12-03 | 昭和電工株式会社 | Novel bacterial alkaline protease and its production method |
DK289083A (en) * | 1983-06-23 | 1984-12-24 | Novo Industri As | LIPASE, PROCEDURE FOR PREPARING THEREOF AND ITS APPLICATION |
GB8514707D0 (en) * | 1985-06-11 | 1985-07-10 | Unilever Plc | Enzymatic detergent composition |
GB8514708D0 (en) * | 1985-06-11 | 1985-07-10 | Unilever Plc | Enzymatic detergent composition |
DK154572C (en) * | 1985-08-07 | 1989-04-24 | Novo Industri As | ENZYMATIC DETERGENT ADDITIVE, DETERGENT AND METHOD FOR WASHING TEXTILES |
GB8629535D0 (en) * | 1986-12-10 | 1987-01-21 | Unilever Plc | Enzymatic detergent composition |
-
1986
- 1986-12-10 GB GB868629536A patent/GB8629536D0/en active Pending
-
1987
- 1987-12-02 EP EP87202386A patent/EP0271154B1/en not_active Expired - Lifetime
- 1987-12-02 DE DE8787202386T patent/DE3763423D1/en not_active Expired - Fee Related
- 1987-12-02 ES ES87202386T patent/ES2016340B3/en not_active Expired - Lifetime
- 1987-12-03 US US07/128,302 patent/US4824599A/en not_active Expired - Fee Related
- 1987-12-08 CA CA000553753A patent/CA1288367C/en not_active Expired - Fee Related
- 1987-12-08 AU AU82224/87A patent/AU607953B2/en not_active Ceased
- 1987-12-09 BR BR8706683A patent/BR8706683A/en not_active IP Right Cessation
- 1987-12-09 KR KR1019870014056A patent/KR920004720B1/en not_active IP Right Cessation
- 1987-12-09 JP JP62311793A patent/JPH0696718B2/en not_active Expired - Lifetime
- 1987-12-10 ZA ZA879298A patent/ZA879298B/en unknown
Also Published As
Publication number | Publication date |
---|---|
AU8222487A (en) | 1988-06-16 |
EP0271154A3 (en) | 1988-08-03 |
EP0271154A2 (en) | 1988-06-15 |
KR880007711A (en) | 1988-08-29 |
GB8629536D0 (en) | 1987-01-21 |
ES2016340B3 (en) | 1990-11-01 |
DE3763423D1 (en) | 1990-08-02 |
KR920004720B1 (en) | 1992-06-15 |
BR8706683A (en) | 1988-07-19 |
US4824599A (en) | 1989-04-25 |
JPS63161085A (en) | 1988-07-04 |
JPH0696718B2 (en) | 1994-11-30 |
CA1288367C (en) | 1991-09-03 |
AU607953B2 (en) | 1991-03-21 |
ZA879298B (en) | 1989-08-30 |
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