EP0030683A1 - Test composition for peroxidatively active substances, test device containing the composition, process for preparing same and method for the determination of peroxidatively active substances - Google Patents

Test composition for peroxidatively active substances, test device containing the composition, process for preparing same and method for the determination of peroxidatively active substances Download PDF

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Publication number
EP0030683A1
EP0030683A1 EP80107598A EP80107598A EP0030683A1 EP 0030683 A1 EP0030683 A1 EP 0030683A1 EP 80107598 A EP80107598 A EP 80107598A EP 80107598 A EP80107598 A EP 80107598A EP 0030683 A1 EP0030683 A1 EP 0030683A1
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Prior art keywords
active substances
peroxidatively active
composition
test
determination
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EP80107598A
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German (de)
French (fr)
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EP0030683B1 (en
Inventor
William I. White
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Bayer Corp
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Miles Laboratories Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • G01N33/725—Haemoglobin using peroxidative activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/904—Oxidation - reduction indicators

Definitions

  • the present invention relates generally to the field of diagnostic tests and, more particularly, to those tests useful in qualitative and quantitative determination of peroxidatively active substances.
  • Patent No. 3,853,472 reports the use of fused polycyclic derivatives of quinoline, such as benzoquinolines and pyridoquinolines, as potentiating or activating agents with similar sensitivities.
  • U.S. Patent No. 3,975,161 Svoboda et al. disclose the use of isoquinolines as potentiators.
  • test compositions include an indicator capable of being oxidized in the presence of peroxidatively active substances to provide a color change, an oxidizing agent effective to oxidize said indicator, and a sensitizing agent.
  • the sensitizing agent comprises 10H-pyrido[3,2-6]-[1,4]benzothiazine.
  • test compositions which are improved by incorporation of the sensitizer described above, further contain at least an indicator and an oxidizing agent and may be prepared in a tablet form or incorporated with a carrier such as an absorbent matrix.
  • Suitable indicators are capable of being oxidized in the presence of a peroxidatively active substance to provide a color change and include well known materials such as 3,3',5,5'-tetramethylbenzidine, o-tolidine, o-toluidine, p-toluidine, o-phenylenediamine, N,N'-dimethyl-p-phenylenediamine, N,N'-diethyl- p-phenylenediamine, benzidine, p-anisidine, dianisidine, o-cresol, m-cresol, p-cresol, alpha-naphthol, beta-naphthol, catechol, guaiacol, pyrogallol or those of the heterocyclic azin
  • oxidizing agents there can be used, for example, cumene hydroperoxide, diisopropylbenzene hydroperoxide, paramenthane hydroperoxide, 2,5-dimethylhexane-2,5-dihydroperoxide and other well known oxidizing agents effective to oxidize the indicators.
  • the improved test compositions of this invention are incorporated on or with a carrier and utilized as a dip and read test device.
  • the test device may be prepared by various well known methods which include impregnating an absorbent carrier material with a solution or solutions of the test composition and thereafter drying the impregnated matrix, thus adhesively incorporating within the matrix a finely divided, intimate mixture of the ingredients.
  • concentration range of sensitizing agent which can be used for impregnation solutions is generally from about 0.05 gram (g)/100 ml to about 1.0 g/100 ml and, preferably, from about 0.1 g/100 ml to about 0.5 g/100 ml.
  • carrier refers to matrices which are insoluble in and maintain their structural integrity when exposed to physiological or other liquids to be tested. Suitable matrices which may be used include paper, cellulose, wood, synthetic resin fleeces, glass fiber, nonwoven and woven fabrics, various organic polymers, such as polypropylene, and other organic materials well known as film formers to those skilled in the art.
  • the carrier may take the form of a pressed or molded tablet containing conventional carrier material.
  • the carrier can be suitably attached to an insoluble support or handle member which can be made from polystyrene.
  • the test device is advantageously used by momentarily dipping it in a test sample or by otherwise introducing a test sample into the carrier matrix, whereby a detectable color change results when the peroxidatively active analyte is present.
  • the volumetric capacity of the carrier serves to limit the amount of sample absorbed thereby and to which the test composition incorporated therewith is exposed. Any excess sample can be removed by washing or blotting the carrier to thereby limit the amount of sample tested to the volume thereof which has actually entered the carrier matrix.
  • the test device can be used in the same way when samples of plasma, serum or other body fluids are tested.
  • Test devices in the form of treated carrier matrices are often stored for considerable periods of time before use, and it is therefore desirable that the reagents chosen are not easily auto-oxidizable in air.
  • the test devices should be protected from exposure to light and in some cases it is desirable to keep them sealed in a moisture repellent package which is opened only for removal of one or more test devices shortly before use.
  • Reflectance readings of color produced by reaction with the peroxidatively active analyte present in the sample can be obtained from commercially available spectrophotometers such as Beckman DK-2 Spectrophotometer, Beckman Instruments, Inc., Fullerton, California or Spectrocolorimeter SCF-l, Israel Electrooptical Industry Ltd. (distributed in the U.S. by Broomer Research Corporation, Plainwell, Long Island, N.Y.).
  • This example reports tests performed to compare the sensitizing agent according to the invention with a known sensitizing agent, 6-methoxy quinoline.
  • a 10 inches x 4 inches piece of Eaton & Dikeman No. 237 paper was impregnated to saturation with the first solution and dried 11 minutes at 95-97°C. Identical pieces of paper were then each impregnated with one of the remaining solutions. They were then cut into pieces 0.2 inch x 0.2 inch and attached to plastic handles.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Urology & Nephrology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Improved test compositions, devices and methods for the detecting of peroxidatively active substances are provided. The test compositions include an indicator capable of being oxidized in the presence of peroxidatively active substances to provide a color change, an oxidizing agent effective to oxidize said indicator, and a sensitizing agent. The sensitizing agent comprises 1 OH-pyrido [3,2-6]-[1,4]benzothiazine.

Description

    BACKGROUND OF THE INVENTION FIELD OF THE INVENTION
  • The present invention relates generally to the field of diagnostic tests and, more particularly, to those tests useful in qualitative and quantitative determination of peroxidatively active substances.
    • DESCRIPTION OF THE PRIOR ART
  • The detection of small amounts of peroxidatively active substances, such as occult blood, hemoglobin, myoglobin, leukocytes, bacteria, or other peroxidatively active analytes, in body fluids and in body excreta has long been recognized as an invaluable aid to the medical practitioner in the diagnosis of many abnormal conditions.
  • Various procedures, compositions and devices are described in the literature for the detection of occult peroxidatively active substances. For example, Kamlet in U.S. Patent No. 2,290,436; Nicholls and Fonner in U.S. Patent No. 2,799,660; Fonner in U.S. Patent No. 2,838,377; and Adams and Peterson in U.S. Patent Nos. 3,012,976, 3,092,463, and 3,092,464, all assigned to the instant assignee, illustrate several test compositions which have been supplied to meet the need for a simple, reliable test for occult blood. These test compositions are based on the peroxidative or catalytic activity of the prosthetic groups present in blood. See also Schwartz, Zeitschrift fur gerichtlich Medizin (Journal of Forensic Medicine) 12: 1928.
  • It has been determined by Adams et al., in U.S. Patent No. 3,290,117 that the sensitivity of these occult blood test compositions can be markedly improved and potentiated by the addition of quinoline or certain quinoline derivatives such as quinine. With the addition of these quinoline derivatives to the prior art occult blood compositions it is possible to detect 5 to 50 red blood cells (RBC)/microliter (µl) of sample which corresponds to a blood dilution as low as 1:1,000,000. Use of the acid addition salts or adducts of quinoline and its derivatives as potentiating agents is disclosed by Mast et at., in U.S. Patent No. 3,986,833. Another approach, disclosed in U.S. Patent No. 3,853,472, reports the use of fused polycyclic derivatives of quinoline, such as benzoquinolines and pyridoquinolines, as potentiating or activating agents with similar sensitivities. In U.S. Patent No. 3,975,161 Svoboda et al., disclose the use of isoquinolines as potentiators.
  • Compounds other than those in the quinoline family have been found effective as well. In U.S. Patent No. 3,917,452 Rittersdorf et aZ., disclose the use of vinyl pyridine compounds for use as sensitizers. In U.S. Patent No. 4,063,894 Ogawa et aZ., disclose the use of substituted thiazole compounds for this purpose.
    • SUMMARY OF THE INVENTION
  • In accordance with the present invention, improved test compositions, devices and methods for the detecting of peroxidatively active substances are provided. The test compositions include an indicator capable of being oxidized in the presence of peroxidatively active substances to provide a color change, an oxidizing agent effective to oxidize said indicator, and a sensitizing agent. The sensitizing agent comprises 10H-pyrido[3,2-6]-[1,4]benzothiazine.
    • DESCRIPTION OF THE PREFERRED EMBODIMENTS
  • Although specific terms are used for clarity, these terms refer only to the embodiments selected for illustration, and are not intended to limit the scope of the invention.
  • The test compositions, which are improved by incorporation of the sensitizer described above, further contain at least an indicator and an oxidizing agent and may be prepared in a tablet form or incorporated with a carrier such as an absorbent matrix. Suitable indicators are capable of being oxidized in the presence of a peroxidatively active substance to provide a color change and include well known materials such as 3,3',5,5'-tetramethylbenzidine, o-tolidine, o-toluidine, p-toluidine, o-phenylenediamine, N,N'-dimethyl-p-phenylenediamine, N,N'-diethyl- p-phenylenediamine, benzidine, p-anisidine, dianisidine, o-cresol, m-cresol, p-cresol, alpha-naphthol, beta-naphthol, catechol, guaiacol, pyrogallol or those of the heterocyclic azine series for example bis-(N-ethyl-quinol-2-one)-azine or (N-methylbenzothiazol-2-one)-(1-ethyl-3-phenyl-5-methtriazol-2-one)-azine. As oxidizing agents there can be used, for example, cumene hydroperoxide, diisopropylbenzene hydroperoxide, paramenthane hydroperoxide, 2,5-dimethylhexane-2,5-dihydroperoxide and other well known oxidizing agents effective to oxidize the indicators.
  • In a preferred embodiment, the improved test compositions of this invention are incorporated on or with a carrier and utilized as a dip and read test device. The test device may be prepared by various well known methods which include impregnating an absorbent carrier material with a solution or solutions of the test composition and thereafter drying the impregnated matrix, thus adhesively incorporating within the matrix a finely divided, intimate mixture of the ingredients. The concentration range of sensitizing agent which can be used for impregnation solutions is generally from about 0.05 gram (g)/100 ml to about 1.0 g/100 ml and, preferably, from about 0.1 g/100 ml to about 0.5 g/100 ml. The term carrier refers to matrices which are insoluble in and maintain their structural integrity when exposed to physiological or other liquids to be tested. Suitable matrices which may be used include paper, cellulose, wood, synthetic resin fleeces, glass fiber, nonwoven and woven fabrics, various organic polymers, such as polypropylene, and other organic materials well known as film formers to those skilled in the art. Alternatively, the carrier may take the form of a pressed or molded tablet containing conventional carrier material. For convenience, the carrier can be suitably attached to an insoluble support or handle member which can be made from polystyrene.
  • The test device is advantageously used by momentarily dipping it in a test sample or by otherwise introducing a test sample into the carrier matrix, whereby a detectable color change results when the peroxidatively active analyte is present. The volumetric capacity of the carrier serves to limit the amount of sample absorbed thereby and to which the test composition incorporated therewith is exposed. Any excess sample can be removed by washing or blotting the carrier to thereby limit the amount of sample tested to the volume thereof which has actually entered the carrier matrix. The test device can be used in the same way when samples of plasma, serum or other body fluids are tested.
  • Test devices in the form of treated carrier matrices are often stored for considerable periods of time before use, and it is therefore desirable that the reagents chosen are not easily auto-oxidizable in air. Advisably, the test devices should be protected from exposure to light and in some cases it is desirable to keep them sealed in a moisture repellent package which is opened only for removal of one or more test devices shortly before use.
  • Reflectance readings of color produced by reaction with the peroxidatively active analyte present in the sample can be obtained from commercially available spectrophotometers such as Beckman DK-2 Spectrophotometer, Beckman Instruments, Inc., Fullerton, California or Spectrocolorimeter SCF-l, Israel Electrooptical Industry Ltd. (distributed in the U.S. by Broomer Research Corporation, Plainwell, Long Island, N.Y.).
  • The following example illustrates preferred embodiments of the invention.
    • Example I
  • This example reports tests performed to compare the sensitizing agent according to the invention with a known sensitizing agent, 6-methoxy quinoline.
  • Three impregnation solutions were prepared as follows:
    • 1. The following compounds were added to 41.67 milliliters (ml) of distilled water in the following order. Each ingredient was dissolved before the next was added.
      Figure imgb0001
  • Then, 41.67 ml N, N-dimethylformamide was added to this solution and then the following compounds were added:
    Figure imgb0002
    • 2. Same, except no 6-methoxy quinoline
    • 3. Substitute 0.210 g. 10H-pyrido[3,2-6]-[1,4]benzothiazine
  • A 10 inches x 4 inches piece of Eaton & Dikeman No. 237 paper was impregnated to saturation with the first solution and dried 11 minutes at 95-97°C. Identical pieces of paper were then each impregnated with one of the remaining solutions. They were then cut into pieces 0.2 inch x 0.2 inch and attached to plastic handles.
  • Solutions of hemoglobin were prepared and the devices prepared above were tested both fresh and after stress storage of 1 week at 60° C. The lowest detectable levels of hemoglobin in urine are reported as milligrams (mg)/ deciliter (dl) in Table I.
    Figure imgb0003
  • These results show that the compounds used in accor- dance with the invention are effective as sensitizers in the detection of peroxidatively active substances, such as occult blood.
  • Although the invention has been described with a certain degree of particularily, numerous changes may be resorted to without departing from the scope of the invention.

Claims (4)

1. A test composition for detection of peroxidatively active substances comprising an indicator capable of being oxidized in the presence of a peroxidatively active substance to provide a color change and an oxidizing agent effective to oxidize said indicator, characterized in that it contains lOH-pyrido [3,2-6]-[1,4]benzothiazine as a sensitizing agent.
2. A test device for detection of peroxidatively active substances which comprises a carrier incorporated with the composition of claim 1.
3. A process for preparing a device for the determination of peroxidatively active substances which comprises incorporating a carrier with the composition of claim 1.
4. A method for the determination of peroxidatively active substance characterized in that said sample is contacted with the composition of claim 1 and any resultant color change is observed.
EP80107598A 1979-12-17 1980-12-04 Test composition for peroxidatively active substances, test device containing the composition, process for preparing same and method for the determination of peroxidatively active substances Expired EP0030683B1 (en)

Applications Claiming Priority (2)

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US06/104,552 US4251222A (en) 1979-12-17 1979-12-17 Sensitizers for peroxidative activity tests
US104552 1979-12-17

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US (1) US4251222A (en)
EP (1) EP0030683B1 (en)
JP (1) JPS5694262A (en)
AU (1) AU520544B2 (en)
CA (1) CA1141635A (en)
DE (1) DE3062644D1 (en)
DK (1) DK533380A (en)
ES (1) ES8204536A1 (en)
FI (1) FI803905L (en)
NO (1) NO803567L (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2905531A1 (en) * 1979-02-14 1981-01-08 Boehringer Mannheim Gmbh DIAGNOSTIC AGENT FOR DETECTING LEUCOCYTES IN BODY LIQUIDS
CA1143634A (en) * 1980-06-02 1983-03-29 Alan E. Burkhardt Interference-resistant test device for determining a peroxidately active substance in a test sample and method for preparing it
US4956300A (en) * 1982-01-05 1990-09-11 Helena Laboratories Corporation Aid for determining the presence of occult blood, method of making the aid, and method of using the aid
US4521511A (en) * 1982-09-22 1985-06-04 Enzyme Technology Company Catalyzed colorimetric and fluorometric substrates for peroxidase enzyme determinations
CA1216215A (en) * 1983-03-01 1987-01-06 Henry J. Wells Peroxidase activity detection composition
US5702913A (en) * 1983-12-21 1997-12-30 Helena Laboratories Corporation Chromgen-reagent test system
US5273888A (en) * 1984-01-16 1993-12-28 Helena Laboratories Corporation Chemical test kit and method for determining the presence of blood in a specimen and for verifying the effectiveness of the chemicals
US4615982A (en) * 1984-12-11 1986-10-07 Lawrence Paul J Fecal occult blood test
US4818702A (en) * 1986-06-02 1989-04-04 Litmus Concepts, Inc. Fecal occult blood test reagent
US5081040A (en) * 1987-06-29 1992-01-14 Helena Laboratories Corporation Composition and kit for testing for occult blood in human and animal excretions, fluids, or tissue matrixes
US5217874A (en) * 1989-04-04 1993-06-08 Helena Laboratories Corporation Fecal occult blood test product with positive and negative controls
US5196167A (en) * 1989-04-04 1993-03-23 Helena Laboratories Corporation Fecal occult blood test product with positive and negative controls
US20070031914A1 (en) * 2005-08-05 2007-02-08 Wei Zhu Devices for analyte assays and methods of use
CN102884429B (en) * 2010-03-01 2014-11-19 ***生物股份有限公司 Test strip for the detection of equol

Citations (1)

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Publication number Priority date Publication date Assignee Title
US4148611A (en) * 1978-06-28 1979-04-10 Miles Laboratories, Inc. Test composition, device and method for the detection of peroxidatively active substances

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DE2235152C2 (en) * 1972-07-18 1975-07-10 Boehringer Mannheim Gmbh, 6800 Mannheim Diagnostic agent for the detection of blood and other peroxidatically active substances in body fluids
AR204182A1 (en) * 1973-12-20 1975-11-28 Boehringer Mannheim Gmbh TEST STRIP FOR VERIFICATION OF PEROXIDIC ACTIVITY SUBSTANCES IN BODY LIQUIDS
CS176664B1 (en) * 1975-02-14 1977-06-30
US3986833A (en) * 1975-09-08 1976-10-19 Miles Laboratories, Inc. Test composition, device, and method for the detection of peroxidatively active substances
JPS5263794A (en) * 1975-11-21 1977-05-26 Shionogi Seiyaku Kk Test piece for latent blood
JPS5424697A (en) * 1977-07-27 1979-02-24 Eiken Chemical Test paper for occult blood test

Patent Citations (1)

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US4148611A (en) * 1978-06-28 1979-04-10 Miles Laboratories, Inc. Test composition, device and method for the detection of peroxidatively active substances

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, Vol. 90, No. 25, June 18, 1979 page 259, Abstract 199872x Columbus, Ohio, USA & JP - A - 79 24697 (EIKEN CHEMICAL CO. LTD.) (24-02-1979) * Whole Abstract * *

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AU6543380A (en) 1981-07-02
EP0030683B1 (en) 1983-04-06
AU520544B2 (en) 1982-02-04
FI803905L (en) 1981-06-18
DK533380A (en) 1981-06-18
JPS6360861B2 (en) 1988-11-25
DE3062644D1 (en) 1983-05-11
US4251222A (en) 1981-02-17
ES497803A0 (en) 1982-05-01
ES8204536A1 (en) 1982-05-01
NO803567L (en) 1981-06-18
JPS5694262A (en) 1981-07-30

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