EP0028644A1 - Verfahren zum bestimmen einer bevorstehenden ovulation - Google Patents
Verfahren zum bestimmen einer bevorstehenden ovulationInfo
- Publication number
- EP0028644A1 EP0028644A1 EP19800901141 EP80901141A EP0028644A1 EP 0028644 A1 EP0028644 A1 EP 0028644A1 EP 19800901141 EP19800901141 EP 19800901141 EP 80901141 A EP80901141 A EP 80901141A EP 0028644 A1 EP0028644 A1 EP 0028644A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- peroxidase
- chromogenic substrate
- test
- vaginal fluid
- fluid sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
Definitions
- This invention relates to methods of detecting impending ovluation in human females, including methods which are sufficiently simple so that a woman can carry out the test on herself without the aid of a physician.
- thermometry the basal body temperature method
- a simple form of the invention contemplates the woman taking a sample of vaginal fluid with, for example, a moistened cotton swab and contacting the swab with a bibulous material comprising a substance which will cause a visible change in the presence of peroxidase and a peroxide. Such visible change could be a change in color or in luminescence.
- the finding that the peroxidase is present in the vaginal fluid makes it.possible for a woman to readily obtain a sample with a moistened cotton swab with no harm to herself.
- One of the preferred forms of the test involves the use of a paper comprising starch and an iodide salt which produces a blue color in the presence of a peroxidase when moistened with hydrogen peroxide.
- the aforesaid test sheet will t rn blue.
- Some of the means used in this invention detect both peroxidase and peroxidase-like substances, such as hemoglobin and its degradation products.
- a simple method for the detection of impending ovulation in women, as in the present invention, is of the highest importance to the human race and is of paramount interest to such prestigious organizations as the Population Council, the Ford Foundation, the National Institutes of Health and the World Health Organ ⁇ ization.
- the present invention a woman can, by herself, determine her impending fertile time.
- the fertile time commences within 12 to 72 hours of ovulation (C. Tietze Fertility and Sterility, Vol. 11, p. 485, I960). ' By abstaining from coitus or by otherwise protecting herself from insemination during the fertile time a woman can avoid pregnancy.
- this form of birth control could, if practiced widely, substantially reduce the rate of world-population growth.
- a woman practicing abstinence during the fertile period as determined by the present invention may avoid the need for contraceptive devices, such as the intrauterine device, for birth control which are considered undesirable to some.
- the present invention may also be an aid to couples who wish to have a child, but have failed because, for example, incorrect timing of coitus.
- a preferred embodiment of the present invention uses a chromogenic substrate which responds rapidly to the estrogen-induced peroxidase of the vagina in the presence of a peroxide, but responds much more slowly to the action of hemoglobin or its degradation products.
- Such chromogenic substrates are characterized by the fact that the rate constant (commonly designated k,; see B. Chance, Advances in Enzymology, 1951, Volume 12, pages 153-180) for the reaction of the substrate with the peroxidase-peroxidide complex exceeds about 10 moles " seconds .
- Substrates having such high values of k. include, for example, p,p biphenol, hydro- quinone and 0-phenylenediamine. It should be noted that values of k. for a given chromogenic substance may differ somewhat with the choice of the particular hydroperoxide employed.
- One of the preferred embodiments involves impreg ⁇ nating a bibulous strip of paper with an inorganic peroxide such as hydrogen peroxide, sodium peroxide, barium peroxide strontium peroxide, sodium perborate, and the like or an organic peroxide, such as methyl hydroperoxide, ethyl hydroperoxide, cumene hydroperoxide, dimethoxy dihydro- peroxy hexane, and the like.
- Hydrogen peroxide may be considered both an inorganic ⁇ peroxrde and as a hydro- peroxide.
- Chromogenic peroxidase substrates which may be employed in the present invention include the following substances:
- -Mo ⁇ oamines such an aniline and its derivatives, orthotoluidine, para-toluidine, etc.
- Diamines such as ortho-phenylenediamine
- Nadi reagent producing a bluish color
- bilirubin producing a greenish color
- iodides which produce a brown color and, if starch is present, produce a deep blue color which is much stronger than iodide alone
- Nadi reagent is such a mixture, namely naphthol and p-phenylenediamine, which gives a better final color than the individual components.
- Another example is a mixture of 4-amino antipyrine and 1,7 dihydroxynaphthaline.
- chromogens notably benzidine and its derivatives give a more intense color if halogen ions, such as iodide and bromide ions, or if halogenoid ions, . such as thiocyanate and selenocynate ions, are present.
- halogen ions such as iodide and bromide ions
- halogenoid ions such as thiocyanate and selenocynate ions
- One of the preferred embodiments of the invention comprises 3,3', 5,5' tetramethyl benzidine and potassium thiocyanate; this mixture yields an intense blue color in a positive test.
- Substrates which change their fluorescence in the presence of a peroxidase and hydrogen peroxide include a loss of fluorescence of scopoletin or a production of fluorescence with, for example, dichlorofluorescin or homovanillic acid. Chemiluminescence is produced in the presence of peroxidases and hydrogen peroxide for the following typical substances, luminol, zinc tetra phenylporphyrine and the like.
- the color forming substance may undergo color change, not as a result of the direct action of a hydroperoxide, but by mediation through another compound which is acted upon by a hydroperoxide and does not itself become highly colored.
- Examples of such color-forming or color-changing substances are:
- Example 5 A woman who has regular menstrual cycles donated vaginal samples daily starting on day 5 of her cycle, where day 1 is taken as the day when menstruation began. The vaginal samples were obtained by the woman using a standard six inch cotton-tipped swab ("Puritan", Hardwood Products Co.). The cotton end was moistened with water and rolled gently on the wall of the anterior vagina. The vaginal sample on the swab was divided into six parts, each part of which was kept moistened.
- OMPI Example 2 A portion of daily vaginal sample as described in example 1 was dipped into a 0.01% aqueous solution of orthotolidine (made up by diluting a 1% ethanolic solution of ortho- tolidine with 0.01 molar phosphate buffer at pH 6.5) and then dipped into a 0.005% solution of hydrogen per ⁇ oxide. Vaginal samples of days 5 through 8 gave no color (i.e. a negative test) but samples of days 9 and 10 gave a strong color (i.e. a positive test). Here again the test presumably anticipated ovulation.
- orthotolidine made up by diluting a 1% ethanolic solution of ortho- tolidine with 0.01 molar phosphate buffer at pH 6.5
- chromogenic substrates such as guaiac, p,p' biphenol or 3,3' 5,5' tetramethylbenzidine.
- Said o-tolidine, p,p' biphenol or tetramethylbenzidine solutions may also comprise a bromide, iodide or thiocyanate.
- a bibulous mat for example paper, comprising them may be employed. After contact with the swab comprising the vaginal sample the bibulous mat is moistened with hydrogen peroxide to produce the color indication if peroxidase is present.
- dichlorfluorescein in place of ortho-tolidine but now a positive test is a strong yellow fluorescence (as obs-erved imder Wood's Lamp illumination) while no or only weak fluorescence is a negative test.
- Example 3 A portion of the daily vaginal sample as described in example 1 was subjected to the commercially-available "Hematest" test for occult blood (Ames Company) .
- the vaginal sample is applied to the bibulous white paper provided, to which a moistened pill containing, according to the manufacturer, ortho-tolidine, strontium peroxide, calcium acetate and tartaric acid is contacted.
- Vaginal samples of days 5 through 8 gave no color or only weak
- Example 4 A portion of the daily vaginal sample as described in example 1 was subjected to the occult blood test portion of the commercially-available "N-Multistix" (Ames Company) . According to the manufacturer this portion of the test stick contains ortho-tolidine and cumene hydroperoxide. The test stick gave no or only weak coloration for vaginal samples of days 5 through 8, but gave a strong blue coloration for samples of days 9 and 10. Here again the test presumably anticipated ovulation.
- a portion of the daily vaginal samples as described in example 1 was subjected to the commercially-available "He occult" occult blood test for feces (Smith Kline and French Laboratories) . According to the manufacturer this consists of a bibulous paper impregnated with an ethanolic solution of guaiac and allowed to dry. The vaginal samples were contacted with " this test paper and then a 0.1% ethanolic solution of hydrogen peroxide was added. The test paper gave no or only weak coloration for vaginal samples of days 5 through 8, but gave a • strong blue coloration for samples of days 9 and 10. Here again the test presumably anticipated ovulation.
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US3927379A | 1979-05-16 | 1979-05-16 | |
US39273 | 1979-05-16 |
Publications (1)
Publication Number | Publication Date |
---|---|
EP0028644A1 true EP0028644A1 (de) | 1981-05-20 |
Family
ID=21904589
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP19800901141 Withdrawn EP0028644A1 (de) | 1979-05-16 | 1980-12-01 | Verfahren zum bestimmen einer bevorstehenden ovulation |
Country Status (2)
Country | Link |
---|---|
EP (1) | EP0028644A1 (de) |
WO (1) | WO1980002596A1 (de) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4614715A (en) * | 1982-05-14 | 1986-09-30 | The Board Of Trustees Of The University Of Illinois | Predictive test for impending ovulation in mammals |
IL71699A0 (en) * | 1983-05-02 | 1984-07-31 | Enzo Biochem Inc | Methods and kits for the analysis and detection of biological or genetic material |
US4615972A (en) * | 1983-11-04 | 1986-10-07 | Immuno Concepts, Inc. | Stabilization of indicators for detecting enzyme activity |
IE59105B1 (en) * | 1985-05-06 | 1994-01-12 | Richardson Vicks Inc | Diagnostic method for detecting periodontal disease |
FR2652092A1 (fr) * | 1989-09-15 | 1991-03-22 | Fama | Procede de mesure de l'activite peroxydase de la glaire cervicale. |
FR2677126B1 (fr) * | 1991-05-27 | 1994-06-03 | Fama Sa | Reactif pour mesurer l'activite peroxydasique de la glaire cervicale, dispositif de mise en óoeuvre de ce reactif et procede de realisation. |
CA2367588A1 (en) * | 1999-04-26 | 2000-11-02 | The Procter & Gamble Company | Panty liner with sensor for predicting the start of menstruation |
CN1099465C (zh) * | 1999-08-25 | 2003-01-22 | 彭晓虹 | 自然避孕检测***试剂盒及制作方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA854156A (en) * | 1970-10-20 | O. Foster Raymond | Test tape and method for detecting peroxidase | |
US3472738A (en) * | 1967-06-12 | 1969-10-14 | Weston Lab Inc | Test implement for detecting peroxidase |
-
1980
- 1980-05-16 WO PCT/US1980/000618 patent/WO1980002596A1/en unknown
- 1980-12-01 EP EP19800901141 patent/EP0028644A1/de not_active Withdrawn
Non-Patent Citations (1)
Title |
---|
See references of WO8002596A1 * |
Also Published As
Publication number | Publication date |
---|---|
WO1980002596A1 (en) | 1980-11-27 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US4340669A (en) | System for the determination of glucose in fluids | |
US4391906A (en) | System for the determination of glucose in fluids | |
US4333734A (en) | Diagnostic device for fecal occult blood and method of use | |
DE69527361T2 (de) | Farbkuppler zur spektrophotometrischen bestimmung von analyten | |
US3968011A (en) | Test implement and test method for colorimetrically determining whether a female is fertile or pregnant | |
US4673654A (en) | Composition for determining peroxidase-like activity of hemoglobin | |
CA1076010A (en) | Determination of isoenzymes | |
DK156667B (da) | Middel og testmateriale til paavisning af tilstedevaerelsen af glucose i en forsoegsproeve | |
EP0028644A1 (de) | Verfahren zum bestimmen einer bevorstehenden ovulation | |
US4724204A (en) | Diagnostic device for the detection of increased dehydrogenase or oxidase and the use thereof | |
EP0074505B1 (de) | Anzeigesystem für Fruchtbarkeit | |
US3699005A (en) | Method and article for detecting the fertile period | |
KR100247941B1 (ko) | 헬리코박터 피롤리의 검출용 조성물, 검출용 키트 및 검출방법 | |
DE3851304T2 (de) | Verfahren und Testzusammensetzung zur Bestimmung von Enzym-Aktivität. | |
US3116223A (en) | Method for detecting the presence of glucose in cervical mucus | |
US5981291A (en) | Estrogen marker system | |
EP0030560A1 (de) | Spezifischer indikator für bevorstehende ovulation | |
CA1252703A (en) | Diagnostic saliva test for detecting periodontal disease | |
Toates | The forensic identification of semen by isoelectric focusing of seminal acid phosphatase | |
CA1276531C (en) | Diagnostic saliva test for detecting periodontal disease | |
FURIYA et al. | Estimation of serum β-acetylaminodeoxyglucosidase | |
Pulkkinen et al. | Pancreatic and catalytic phospholipase A2 in relation to pregnancy, labor and fetal outcome | |
GB2033577A (en) | Recovery and identification of microfilariae | |
MXPA97006716A (en) | Chemical time regulator for pru reagent visual strip |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
AK | Designated contracting states |
Designated state(s): AT CH DE FR GB LU NL SE |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 19810518 |