EA201991665A3 - TOXIN GENES AXMI477, AXMI482, AXMI486 AND AXMI525 AND METHODS OF THEIR APPLICATION - Google Patents

TOXIN GENES AXMI477, AXMI482, AXMI486 AND AXMI525 AND METHODS OF THEIR APPLICATION

Info

Publication number
EA201991665A3
EA201991665A3 EA201991665A EA201991665A EA201991665A3 EA 201991665 A3 EA201991665 A3 EA 201991665A3 EA 201991665 A EA201991665 A EA 201991665A EA 201991665 A EA201991665 A EA 201991665A EA 201991665 A3 EA201991665 A3 EA 201991665A3
Authority
EA
Eurasian Patent Office
Prior art keywords
plants
compositions
amino acid
bacteria
methods
Prior art date
Application number
EA201991665A
Other languages
Russian (ru)
Other versions
EA201991665A2 (en
Inventor
Дуэйн Алан Лехтинен
Кимберли С. Сэмпсон
Кира Робертс
Итан Данн
Нана Шугюль
Original Assignee
Басф Агрикалчерал Солюшнс Сид Юс Ллк
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Басф Агрикалчерал Солюшнс Сид Юс Ллк filed Critical Басф Агрикалчерал Солюшнс Сид Юс Ллк
Publication of EA201991665A2 publication Critical patent/EA201991665A2/en
Publication of EA201991665A3 publication Critical patent/EA201991665A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

Обеспечиваются композиции и способы для придания пестицидной активности бактериям, растениям, растительным клеткам, тканям и семенам. Обеспечиваются композиции, содержащие последовательность, кодирующую полипептид токсина. Кодирующие последовательности можно применять в ДНК-конструктах или кассетах экспрессии для трансформации и экспрессии в растениях и бактериях. Композиции также содержат трансформированные бактерии, растения, растительные клетки, ткани и семена. В частности, обеспечиваются выделенные молекулы нуклеиновой кислоты токсина. Дополнительно охвачены аминокислотные последовательности, соответствующие данным полинуклеотидам, и антитела, специфически связывающиеся с данными аминокислотными последовательностями. В частности, в настоящем изобретении предложены выделенные молекулы нуклеиновой кислоты, которые содержат нуклеотидные последовательности, кодирующие аминокислотную последовательность, показанную в SEQ ID NO: 5-26, или нуклеотидные последовательности, изложенные в SEQ ID NO: 1-4, а также их варианты и фрагменты.Compositions and methods are provided for imparting pesticidal activity to bacteria, plants, plant cells, tissues, and seeds. Compositions are provided comprising a toxin polypeptide coding sequence. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. The compositions also contain transformed bacteria, plants, plant cells, tissues and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally encompassed are amino acid sequences corresponding to these polynucleotides and antibodies that specifically bind to these amino acid sequences. In particular, the present invention provides isolated nucleic acid molecules that contain nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 5-26 or nucleotide sequences set forth in SEQ ID NO: 1-4, as well as variants thereof, and fragments.

EA201991665A 2013-12-09 2014-12-08 TOXIN GENES AXMI477, AXMI482, AXMI486 AND AXMI525 AND METHODS OF THEIR APPLICATION EA201991665A3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201361913911P 2013-12-09 2013-12-09

Publications (2)

Publication Number Publication Date
EA201991665A2 EA201991665A2 (en) 2020-05-31
EA201991665A3 true EA201991665A3 (en) 2020-07-31

Family

ID=70847660

Family Applications (2)

Application Number Title Priority Date Filing Date
EA202192538A EA202192538A2 (en) 2013-12-09 2014-12-08 AXMI477, AXMI482, AXMI486 AND AXMI525 TOXIN GENES AND METHODS OF THEIR APPLICATION
EA201991665A EA201991665A3 (en) 2013-12-09 2014-12-08 TOXIN GENES AXMI477, AXMI482, AXMI486 AND AXMI525 AND METHODS OF THEIR APPLICATION

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EA202192538A EA202192538A2 (en) 2013-12-09 2014-12-08 AXMI477, AXMI482, AXMI486 AND AXMI525 TOXIN GENES AND METHODS OF THEIR APPLICATION

Country Status (1)

Country Link
EA (2) EA202192538A2 (en)

Also Published As

Publication number Publication date
EA201991665A2 (en) 2020-05-31
EA202192538A2 (en) 2021-12-31

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