EA201991443A1 - ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9 - Google Patents

ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9

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Publication number
EA201991443A1
EA201991443A1 EA201991443A EA201991443A EA201991443A1 EA 201991443 A1 EA201991443 A1 EA 201991443A1 EA 201991443 A EA201991443 A EA 201991443A EA 201991443 A EA201991443 A EA 201991443A EA 201991443 A1 EA201991443 A1 EA 201991443A1
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Prior art keywords
cas protein
relates
polypeptide
cas9 nucleases
genetic engineering
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EA201991443A
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English (en)
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Джон Ван Дер Ост
Рихард Ван Краненбург
Эллеке Фенна Босма
Иоаннис Маугиакос
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Вагенинген Университейт
Стихтинг Вор Де Технише Ветенсхаппен
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Publication of EA201991443A1 publication Critical patent/EA201991443A1/ru

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Abstract

Термостабильные нуклеазы Cas9. Настоящее изобретение относится к области генной инженерии и, в частности, к редактированию нуклеиновых кислот и модификации генома. Настоящее изобретение относится к выделенному белку Cas или его полипептидному фрагменту, имеющему аминокислотную последовательность SEQ ID NO: 1 или последовательность, идентичную ей по меньшей мере на 77%. Белок или полипептид Cas способен к связыванию, расщеплению, маркированию или модификации двухцепочечного полинуклеотида-мишени при температуре в диапазоне от 30 до 100°C включительно. Изобретение также относится к выделенным молекулам нуклеиновой кислоты, кодирующим указанные нуклеазы Cas9, экспрессионным векторам и клеткам-хозяевам. По изобретению также предложены последовательности PAM, узнаваемые белком или полипептидом Cas. Нуклеазы Cas9, раскрытые в настоящем документе, являются новыми инструментами для генетической инженерии при повышенных температурах и имеют особую ценность при генетических манипуляциях с термофильными организмами, в частности микроорганизмами.
EA201991443A 2016-12-14 2016-12-14 ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9 EA201991443A1 (ru)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2016/081077 WO2018108272A1 (en) 2016-12-14 2016-12-14 Thermostable cas9 nucleases

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EA201991443A1 true EA201991443A1 (ru) 2020-01-14

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EA201991443A EA201991443A1 (ru) 2016-12-14 2016-12-14 ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9
EA201991442A EA201991442A1 (ru) 2016-12-14 2017-08-16 ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9
EA201991440A EA201991440A1 (ru) 2016-12-14 2017-08-16 ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9
EA201991441A EA201991441A1 (ru) 2016-12-14 2017-12-14 ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9

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EA201991442A EA201991442A1 (ru) 2016-12-14 2017-08-16 ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9
EA201991440A EA201991440A1 (ru) 2016-12-14 2017-08-16 ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9
EA201991441A EA201991441A1 (ru) 2016-12-14 2017-12-14 ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9

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US (5) US11242513B2 (ru)
EP (4) EP3555275A1 (ru)
JP (6) JP7182545B2 (ru)
KR (4) KR20190104342A (ru)
CN (4) CN110312792B (ru)
AU (4) AU2016432443B2 (ru)
BR (4) BR112019012155A2 (ru)
CA (4) CA3046824A1 (ru)
EA (4) EA201991443A1 (ru)
PH (4) PH12019501344A1 (ru)
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