EA201692327A1 - Пролин-специфическая эндопротеаза - Google Patents

Пролин-специфическая эндопротеаза

Info

Publication number
EA201692327A1
EA201692327A1 EA201692327A EA201692327A EA201692327A1 EA 201692327 A1 EA201692327 A1 EA 201692327A1 EA 201692327 A EA201692327 A EA 201692327A EA 201692327 A EA201692327 A EA 201692327A EA 201692327 A1 EA201692327 A1 EA 201692327A1
Authority
EA
Eurasian Patent Office
Prior art keywords
polypeptide
sequence
seq
presented
mature polypeptide
Prior art date
Application number
EA201692327A
Other languages
English (en)
Other versions
EA034253B1 (ru
Inventor
Ван Дер Ян Метске Лан
Ван Де Петер Йозеф Ида Вондерворт
Шанталь Кристис
Мартине Спанс
Де Ангела Брёйне-Паулус
Йоханна Хенрика Гердина Мария Мутсаэрс
Original Assignee
ДСМ АйПи АССЕТС Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ДСМ АйПи АССЕТС Б.В. filed Critical ДСМ АйПи АССЕТС Б.В.
Publication of EA201692327A1 publication Critical patent/EA201692327A1/ru
Publication of EA034253B1 publication Critical patent/EA034253B1/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
    • A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
    • A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
    • A23J3/347—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of proteins from microorganisms or unicellular algae
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A23L2/66—Proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/195—Proteins from microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
    • A23L7/10—Cereal-derived products
    • A23L7/101—Addition of antibiotics, vitamins, amino-acids, or minerals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21026—Prolyl oligopeptidase (3.4.21.26), i.e. proline-specific endopeptidase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Fodder In General (AREA)
  • Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Non-Alcoholic Beverages (AREA)

Abstract

Настоящее изобретение относится к полипептиду, обладающему активностью пролин-специфической эндопротеазы, выбранному из группы, состоящей из: i) полипептида, содержащего последовательность зрелого полипептида, представленную в SEQ ID NO: 2; ii) полипептида, который имеет последовательность, по меньшей мере на 70, 75, 80, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98 или 99% идентичную последовательности зрелого полипептида, представленной в SEQ ID NO: 2; iii) полипептида, кодируемого нуклеиновой кислотой, которая гибридизуется в условиях средней жесткости, предпочтительно в условиях высокой жесткости с цепью, последовательность которой комплементарна последовательности, кодирующей зрелый полипептид, представленной в SEQ ID NO: 1; iv) полипептида, кодируемого нуклеиновой кислотой, которая имеет последовательность, по меньшей мере на 70, 75, 80, 85, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99 или 100% идентичную последовательности, кодирующей зрелый полипептид, представленной в SEQ ID NO: 1. Кроме того, изобретение относится к способу получения полипептида и процессу производства пищевого или кормового продукта, в котором применяют полипептид.
EA201692327A 2014-05-19 2015-05-19 Пролин-специфическая эндопротеаза EA034253B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14168866 2014-05-19
PCT/EP2015/061026 WO2015177171A1 (en) 2014-05-19 2015-05-19 Proline-specific endoprotease

Publications (2)

Publication Number Publication Date
EA201692327A1 true EA201692327A1 (ru) 2017-02-28
EA034253B1 EA034253B1 (ru) 2020-01-22

Family

ID=50732004

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201692327A EA034253B1 (ru) 2014-05-19 2015-05-19 Пролин-специфическая эндопротеаза

Country Status (11)

Country Link
US (1) US10450554B2 (ru)
EP (1) EP3146043B1 (ru)
JP (1) JP6601630B2 (ru)
CN (1) CN106459947B (ru)
BR (1) BR112016026789B1 (ru)
DK (1) DK3146043T3 (ru)
EA (1) EA034253B1 (ru)
ES (1) ES2744898T3 (ru)
MX (1) MX2016015036A (ru)
PL (1) PL3146043T3 (ru)
WO (1) WO2015177171A1 (ru)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015185590A1 (en) * 2014-06-03 2015-12-10 Dsm Ip Assets B.V. Proline-specific endoprotease and use thereof
US20200063166A1 (en) 2017-03-13 2020-02-27 Dsm Ip Assets B.V. Zinc binuclear cluster transcriptional regulator-deficient strain
WO2023222614A1 (en) 2022-05-16 2023-11-23 Dsm Ip Assets B.V. Lipolytic enzyme variants

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1151762A (zh) 1994-06-30 1997-06-11 诺沃诺尔迪斯克生物技术有限公司 非毒性、非产毒性、非致病性镰孢属表达***及所用启动子和终止子
CN1169961C (zh) 1997-04-11 2004-10-06 Dsm公司 基因转变作为工具用于构建重组的工业化丝状真菌
AU736111C (en) 1997-12-22 2004-02-19 Dsm Ip Assets B.V. Expression cloning in filamentous fungi
EP2278016B1 (en) 1999-03-22 2012-09-26 Novozymes Inc. Promoter sequences derived from Fusarium Venenatum and uses thereof
AU2002234561B2 (en) * 2000-12-07 2007-01-18 Dsm Ip Assets B.V. Protein hydrolysates enriched in peptides having a carboxy terminal proline residue
CA2431291C (en) * 2000-12-07 2010-09-21 Dsm N.V. Method for the prevention or reduction of haze in beverages
AU2003215272B2 (en) 2002-02-14 2008-04-03 The Board Of Trustees Of The Leland Stanford Junior University Enzyme treatment of foodstuffs for celiac sprue
DK1511835T5 (en) * 2002-06-07 2015-05-18 Dsm Ip Assets Bv IMPROVED PROCEDURE FOR PREVENTION OR REDUCTION OF BEVERAGES
RU2370279C2 (ru) 2003-09-23 2009-10-20 ДСМ Ай Пи ЭССЕТС Б.В. Применение пролинспецифичных эндопротеаз для гидролиза пептидов и белков
CA2595056A1 (en) 2005-01-24 2006-07-27 Dsm Ip Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
DK2423315T3 (en) 2006-06-29 2015-04-13 Dsm Ip Assets Bv A method for obtaining enhanced polypeptide expression
CA2647327C (en) 2006-07-13 2016-02-23 Dsm Ip Assets B.V. Improved brewing process
CN101925674A (zh) * 2007-11-23 2010-12-22 帝斯曼知识产权资产管理有限公司 改进的生物活性肽生产
TW201000634A (en) 2008-05-30 2010-01-01 Dsm Ip Assets Bv Proline-specific protease
AU2010241099A1 (en) 2009-04-22 2011-10-27 Dsm Ip Assets B.V. Process for the production of a recombinant polypeptide of interest
JP2012533312A (ja) 2009-07-22 2012-12-27 ディーエスエム アイピー アセッツ ビー．ブイ． 目的の化合物を産生するための改善された宿主細胞
WO2012174127A1 (en) * 2011-06-17 2012-12-20 Alvine Pharmaceuticals, Inc. Proteases for degrading gluten
WO2013007820A1 (en) 2011-07-14 2013-01-17 Dsm Ip Assets B.V. Screening method
US9631197B2 (en) * 2012-03-12 2017-04-25 Dsm Ip Assets B.V. Rasamsonia transformants
US20150064765A1 (en) 2012-04-23 2015-03-05 Dsm Ip Assets B.V. Polypeptide expression method
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof

Also Published As

Publication number Publication date
MX2016015036A (es) 2017-05-02
BR112016026789A2 (pt) 2017-12-12
CN106459947A (zh) 2017-02-22
DK3146043T3 (da) 2019-09-23
PL3146043T3 (pl) 2019-12-31
BR112016026789B1 (pt) 2023-05-02
JP6601630B2 (ja) 2019-11-06
EP3146043B1 (en) 2019-06-26
US10450554B2 (en) 2019-10-22
US20170081651A1 (en) 2017-03-23
CN106459947B (zh) 2024-05-14
JP2017515467A (ja) 2017-06-15
EP3146043A1 (en) 2017-03-29
WO2015177171A1 (en) 2015-11-26
ES2744898T3 (es) 2020-02-26
EA034253B1 (ru) 2020-01-22

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