EA201590794A8 - Рекомбинантные нейротоксины clostridium botulinum - Google Patents

Рекомбинантные нейротоксины clostridium botulinum

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Publication number
EA201590794A8
EA201590794A8 EA201590794A EA201590794A EA201590794A8 EA 201590794 A8 EA201590794 A8 EA 201590794A8 EA 201590794 A EA201590794 A EA 201590794A EA 201590794 A EA201590794 A EA 201590794A EA 201590794 A8 EA201590794 A8 EA 201590794A8
Authority
EA
Eurasian Patent Office
Prior art keywords
contiguous nucleotides
bont
clostridium botulinum
sequence
stranded
Prior art date
Application number
EA201590794A
Other languages
English (en)
Other versions
EA201590794A1 (ru
Inventor
Эйми Коссинс
Мэттью Биэрд
Маркс Филип
Original Assignee
Синтаксин Лимитед
Ипсен Байофарм Лимитед
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Синтаксин Лимитед, Ипсен Байофарм Лимитед filed Critical Синтаксин Лимитед
Publication of EA201590794A1 publication Critical patent/EA201590794A1/ru
Publication of EA201590794A8 publication Critical patent/EA201590794A8/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/164—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/4886—Metalloendopeptidases (3.4.24), e.g. collagenase
    • A61K38/4893—Botulinum neurotoxin (3.4.24.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24069—Bontoxilysin (3.4.24.69), i.e. botulinum neurotoxin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biophysics (AREA)
  • Dermatology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Изобретение относится к последовательности нуклеиновой кислоты, содержащей последовательность смежных нуклеотидов, где указанная последовательность смежных нуклеотидов обладает по меньшей мере 90% идентичностью последовательности нуклеиновой кислоты SEQ ID NO: 1 и где указанная последовательность смежных нуклеотидов кодирует одноцепочечный белок BoNT/E1. Изобретение также относится к способам получения растворимого одноцепочечного белка BoNT/E в клетке-хозяине Е.coli вместе со способами получения растворимого двухцепочечного белка BoNT/E1.
EA201590794A 2012-10-31 2013-10-31 Рекомбинантные нейротоксины clostridium botulinum EA201590794A8 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB201219602A GB201219602D0 (en) 2012-10-31 2012-10-31 Recombinant clostridium botulinum neurotoxins
PCT/GB2013/052845 WO2014068317A1 (en) 2012-10-31 2013-10-31 Recombinant clostridium botulinum neurotoxins

Publications (2)

Publication Number Publication Date
EA201590794A1 EA201590794A1 (ru) 2015-08-31
EA201590794A8 true EA201590794A8 (ru) 2016-01-29

Family

ID=47358958

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201590794A EA201590794A8 (ru) 2012-10-31 2013-10-31 Рекомбинантные нейротоксины clostridium botulinum

Country Status (27)

Country Link
US (1) US10030238B2 (ru)
EP (3) EP3326644B1 (ru)
JP (2) JP2019068823A (ru)
KR (2) KR102188539B1 (ru)
CN (2) CN110499321A (ru)
AR (2) AR093309A1 (ru)
AU (3) AU2013340610B2 (ru)
BR (1) BR112015005384A2 (ru)
CA (1) CA2885519A1 (ru)
DK (2) DK2914282T3 (ru)
EA (1) EA201590794A8 (ru)
ES (2) ES2662402T3 (ru)
GB (1) GB201219602D0 (ru)
HK (1) HK1208359A1 (ru)
HU (2) HUE036764T2 (ru)
IL (1) IL237623B (ru)
IN (1) IN2015MN00436A (ru)
MX (2) MX367080B (ru)
NO (1) NO2914282T3 (ru)
NZ (1) NZ705575A (ru)
PL (2) PL3326644T3 (ru)
PT (2) PT2914282T (ru)
SG (2) SG11201502372SA (ru)
TW (3) TW202012622A (ru)
UA (1) UA118837C2 (ru)
WO (1) WO2014068317A1 (ru)
ZA (1) ZA201501449B (ru)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201219602D0 (en) * 2012-10-31 2012-12-12 Syntaxin Ltd Recombinant clostridium botulinum neurotoxins
CN115925835A (zh) * 2015-03-26 2023-04-07 哈佛大学校长及研究员协会 工程改造的肉毒杆菌神经毒素
WO2017063743A1 (en) 2015-10-14 2017-04-20 Merz Pharma Gmbh & Co. Kgaa Improvements to ultrasound-based therapy of photoaged tissue
SG11201805195YA (en) 2016-03-02 2018-07-30 Merz Pharma Gmbh & Co Kgaa Composition comprising botulinum toxin
EP3263710A1 (en) * 2016-07-01 2018-01-03 Ipsen Biopharm Limited Production of activated clostridial neurotoxins
CN110072880A (zh) 2016-08-24 2019-07-30 哈佛大学校长及研究员协会 工程化肉毒杆菌神经毒素
KR20240023684A (ko) 2016-09-13 2024-02-22 알레간 인코포레이티드 안정화된 비단백질 클로스트리듐 독소 조성물
EP3642222A1 (en) 2017-06-20 2020-04-29 Merz Pharma GmbH & Co. KGaA Novel recombinant botulinum toxin with increased duration of effect
WO2019007509A1 (en) 2017-07-06 2019-01-10 Merz Pharma Gmbh & Co. Kgaa NOVEL RECOMBINANT BOTULINUM NEUROTOXINS WITH INCREASED DURATION
BR112020006827A2 (pt) * 2018-01-29 2020-10-06 Ipsen Biopharm Limited neurotoxinas botulínicas clivando snare não neuronal
EP3752128A1 (de) * 2018-02-16 2020-12-23 Bontana Therapies Gmbh Nukleinsäure-basiertes botulinum neurotoxin zur therapeutischen anwendung
GB201815844D0 (en) * 2018-09-28 2018-11-14 Ipsen Biopharm Ltd Therapeutic & comestic uses of botulinum neurotoxin serotype e
GB202011055D0 (en) 2020-07-17 2020-09-02 Ipsen Bioinnovation Ltd Treatment of post-operative pain
GB202015618D0 (en) 2020-10-01 2020-11-18 Ipsen Biopharm Ltd Method for producing beta-trypsin
CN115819526A (zh) * 2022-12-02 2023-03-21 海雅美生物技术(珠海)有限公司 一种重组肉毒杆菌神经毒素及其制备方法和应用

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6967088B1 (en) 1995-03-16 2005-11-22 Allergan, Inc. Soluble recombinant botulinum toxin proteins
NZ308772A (en) * 1995-05-17 1999-04-29 Du Pont Recombinant baculovirus insecticides
AU759461B2 (en) 1998-07-10 2003-04-17 U.S. Medical Research Institute Of Infectious Diseases Botulinum neurotoxin vaccine
DE60032367T3 (de) 1999-08-25 2011-03-10 Allergan, Inc., Irvine Aktivierbare rekombinante neurotoxine
US7374896B2 (en) 2001-08-28 2008-05-20 Allergan, Inc. GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity
WO2006096164A1 (en) * 2005-03-03 2006-09-14 Allergan, Inc. Media for clostridium bacterium and processes for obtaining a clostridial toxin
US7514088B2 (en) * 2005-03-15 2009-04-07 Allergan, Inc. Multivalent Clostridial toxin derivatives and methods of their use
US20110111483A1 (en) * 2004-06-30 2011-05-12 Allergan, Inc. Optimizing Expression of Active Botulinum Toxin Type E
WO2006011966A1 (en) * 2004-06-30 2006-02-02 Allergan, Inc. Optimizing expression of active botulinum toxin type e
GB2416122A (en) * 2004-07-12 2006-01-18 Ipsen Ltd Botulinum neurotoxin composition
DE102004043009A1 (de) * 2004-09-06 2006-03-23 Toxogen Gmbh Transportprotein zum Einbringen chemischer Verbindungen in Nervenzellen
DE102005002978B4 (de) * 2005-01-21 2013-04-25 Merz Pharma Gmbh & Co. Kgaa Rekombinante Expression von Proteinen in einer disulfidverbrückten, zweikettigen Form
DK1926744T4 (en) * 2005-09-19 2019-01-28 Allergan Inc CLOSTRIDIUM TOXIN-ACTIVABLE CLOSTRIDIAL TOXINES
US20070218084A1 (en) * 2005-12-30 2007-09-20 Fondazione Pierfranco E Luisa Mariani- Onlus Method for the Mapping of the Epileptogenic Focus in the Pre-Surgical Evaluation of Patients with Intractable Epilepsy
EP2038299A2 (en) 2006-07-11 2009-03-25 Allergan, Inc. Modified clostridial toxins with enhanced translocation capabilities and altered targeting activity for non-clostridial toxin target cells
EP2178905B1 (en) * 2007-07-26 2013-11-06 Allergan, Inc. Methods of activiting clostridial toxins
CN105833254A (zh) 2008-12-10 2016-08-10 阿勒根公司 梭菌毒素药物组合物
GB0903006D0 (en) * 2009-02-23 2009-04-08 Syntaxin Ltd Modified non-cytotoxic proteases
EP2419128B1 (en) * 2009-04-14 2018-06-06 Medical College of Wisconsin, Inc. Engineered botulinum neurotoxin
KR20120107988A (ko) * 2009-12-16 2012-10-04 알러간, 인코포레이티드 통합된 프로테아제 절단 부위 결합 도메인을 포함하는 변형 클로스트리듐 독소
US20120207743A1 (en) 2011-02-14 2012-08-16 Allergan, Inc. Inhibiting Aberrant Blood Vessel Formation Using Retargeted Endopeptidases
GB201219602D0 (en) * 2012-10-31 2012-12-12 Syntaxin Ltd Recombinant clostridium botulinum neurotoxins
GB201407525D0 (en) * 2014-04-29 2014-06-11 Syntaxin Ltd Manufacture of recombinant clostridium botulinum neurotoxins
CN110072880A (zh) * 2016-08-24 2019-07-30 哈佛大学校长及研究员协会 工程化肉毒杆菌神经毒素

Also Published As

Publication number Publication date
MX367080B (es) 2019-08-05
AU2020244481A1 (en) 2020-10-29
GB201219602D0 (en) 2012-12-12
PL3326644T3 (pl) 2020-08-24
PT3326644T (pt) 2020-05-29
PL2914282T3 (pl) 2018-07-31
EP3326644A1 (en) 2018-05-30
DK2914282T3 (en) 2018-02-26
EP3673914B1 (en) 2021-10-27
TWI673361B (zh) 2019-10-01
CN110499321A (zh) 2019-11-26
TW202012622A (zh) 2020-04-01
AU2018203556B2 (en) 2020-08-20
EP3673914A1 (en) 2020-07-01
KR20200110470A (ko) 2020-09-23
HK1208359A1 (en) 2016-03-04
HUE048802T2 (hu) 2020-08-28
KR102264478B1 (ko) 2021-06-15
TWI626307B (zh) 2018-06-11
AR093309A1 (es) 2015-05-27
SG11201502372SA (en) 2015-05-28
IL237623B (en) 2019-05-30
IN2015MN00436A (ru) 2015-09-04
ES2788199T3 (es) 2020-10-20
CN104755098A (zh) 2015-07-01
PT2914282T (pt) 2018-03-13
NO2914282T3 (ru) 2018-05-19
WO2014068317A1 (en) 2014-05-08
IL237623A0 (en) 2015-04-30
UA118837C2 (uk) 2019-03-25
JP2020078321A (ja) 2020-05-28
ES2662402T3 (es) 2018-04-06
HUE036764T2 (hu) 2018-07-30
MX2015005156A (es) 2015-09-23
AU2018203556A1 (en) 2018-06-07
AU2013340610B2 (en) 2018-02-22
DK3326644T3 (da) 2020-05-11
CA2885519A1 (en) 2014-05-08
EA201590794A1 (ru) 2015-08-31
ZA201501449B (en) 2016-08-31
EP2914282B1 (en) 2017-12-20
MX2019009222A (es) 2019-11-21
BR112015005384A2 (pt) 2017-08-08
EP2914282A1 (en) 2015-09-09
KR20150094590A (ko) 2015-08-19
EP3326644B1 (en) 2020-03-04
AR117738A2 (es) 2021-08-25
NZ705575A (en) 2019-08-30
SG10201701140WA (en) 2017-04-27
AU2013340610A1 (en) 2015-03-19
JP2019068823A (ja) 2019-05-09
US20150247139A1 (en) 2015-09-03
US10030238B2 (en) 2018-07-24
TW201825680A (zh) 2018-07-16
KR102188539B1 (ko) 2020-12-09
TW201435084A (zh) 2014-09-16

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