EA201390638A1 - Обогащение и идентификация фетальных клеток в материнской крови и лиганды для такого применения - Google Patents

Обогащение и идентификация фетальных клеток в материнской крови и лиганды для такого применения

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Publication number
EA201390638A1
EA201390638A1 EA201390638A EA201390638A EA201390638A1 EA 201390638 A1 EA201390638 A1 EA 201390638A1 EA 201390638 A EA201390638 A EA 201390638A EA 201390638 A EA201390638 A EA 201390638A EA 201390638 A1 EA201390638 A1 EA 201390638A1
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Eurasian Patent Office
Prior art keywords
fetal cells
ligands
identification
maternal blood
entrance
Prior art date
Application number
EA201390638A
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English (en)
Other versions
EA027314B1 (ru
EA201390638A8 (ru
Inventor
Андреас Эккельт
Бритта Кристенсен
Стен Кельро
Мари Бринч
Сингх Рипудаман
Лотте Хатт
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Квантибакт А/С
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Publication date
Application filed by Квантибакт А/С filed Critical Квантибакт А/С
Publication of EA201390638A1 publication Critical patent/EA201390638A1/ru
Publication of EA201390638A8 publication Critical patent/EA201390638A8/ru
Publication of EA027314B1 publication Critical patent/EA027314B1/ru

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
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    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
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    • C12Q2600/00—Oligonucleotides characterized by their use
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    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
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    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4742—Keratin; Cytokeratin
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70596—Molecules with a "CD"-designation not provided for elsewhere in G01N2333/705

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Настоящее изобретение относится к обогащению и/или идентификации фетальных клеток в пробе материнской крови с использованием лигандов, специфичных для фетальных клеток, и/или гибридизационных зондов, специфичных для фетальных клеток, где лиганды или зонды в первом раунде обогащения направлены на эндотелиальный/мезинхимальный маркер, например на CD105, CD146 или CD141, а во втором раунде обогащения они направлены на эпителиальный маркер, например на цитокератин, такой как CK7, CK8, CK18 или CK19. Обогащенные или идентифицированные фетальные клетки могут быть подвергнуты стадиям обнаружения или диагностирования, поэтому настоящее изобретение обеспечивает 5 способов неинвазивной пренатальной диагностики.
EA201390638A 2010-11-09 2011-11-09 Способы выделения фетальной клетки с эндотелиальными и эпителиальными свойствами и набор для их осуществления EA027314B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA201001018 2010-11-09
PCT/DK2011/050423 WO2012062325A1 (en) 2010-11-09 2011-11-09 Enrichment and identification of fetal cells in maternal blood and ligands for such use

Publications (3)

Publication Number Publication Date
EA201390638A1 true EA201390638A1 (ru) 2013-09-30
EA201390638A8 EA201390638A8 (ru) 2014-01-30
EA027314B1 EA027314B1 (ru) 2017-07-31

Family

ID=45001595

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201390638A EA027314B1 (ru) 2010-11-09 2011-11-09 Способы выделения фетальной клетки с эндотелиальными и эпителиальными свойствами и набор для их осуществления

Country Status (21)

Country Link
US (1) US9429520B2 (ru)
EP (1) EP2638176B1 (ru)
JP (2) JP6068354B2 (ru)
KR (2) KR20130102612A (ru)
CN (1) CN103261439B (ru)
CA (1) CA2817390C (ru)
CY (1) CY1119526T1 (ru)
DK (1) DK2638176T3 (ru)
EA (1) EA027314B1 (ru)
ES (1) ES2642313T3 (ru)
HR (1) HRP20171468T1 (ru)
HU (1) HUE034937T2 (ru)
IL (1) IL226188A (ru)
LT (1) LT2638176T (ru)
PL (1) PL2638176T3 (ru)
PT (1) PT2638176T (ru)
RS (1) RS56402B1 (ru)
SG (2) SG190175A1 (ru)
SI (1) SI2638176T1 (ru)
WO (1) WO2012062325A1 (ru)
ZA (1) ZA201303148B (ru)

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SG190175A1 (en) * 2010-11-09 2013-06-28 Quantibact As Enrichment and identification of fetal cells in maternal blood and ligands for such use
WO2014067528A1 (en) 2012-10-29 2014-05-08 Arcedi Biotech Aps Improved enrichment and detection of rare blood cells
CN106061998B (zh) * 2014-02-05 2020-12-11 弗吉瑞必欧诊断股份公司 用于检测恶性赘生性疾病的组合物和方法
WO2016021311A1 (ja) * 2014-08-05 2016-02-11 富士フイルム株式会社 有核赤血球の選別方法
CA2974373A1 (en) * 2015-01-23 2016-07-28 Unimed Biotech (Shanghai) Co., Ltd. Microfluidics based fetal cell detection and isolation for non-invasive prenatal testing
CN104977284B (zh) * 2015-07-03 2018-05-29 石莹 一种胎儿有核红细胞的捕获及鉴定方法
CN107475354A (zh) * 2016-06-08 2017-12-15 益善生物技术股份有限公司 ***癌组织溯源ctc分型鉴定试剂盒
CN107475358A (zh) * 2016-06-08 2017-12-15 益善生物技术股份有限公司 结直肠癌组织溯源ctc分型鉴定试剂盒
CN107475353A (zh) * 2016-06-08 2017-12-15 益善生物技术股份有限公司 肺癌组织溯源ctc分型鉴定试剂盒
US20180299442A1 (en) * 2017-04-14 2018-10-18 Jennifer C. Chow Enrichment and identification of fetal material
CN111122520A (zh) * 2018-10-31 2020-05-08 四川大学华西第二医院 一种胚胎植入检测试剂盒及其应用和使用方法
JP6876287B2 (ja) 2019-01-30 2021-05-26 大日本印刷株式会社 小腸上皮細胞を含む細胞構造物、その製造のための方法、及び、それを保持する基材
PL3980773T3 (pl) * 2019-06-07 2023-11-27 Arcedi Biotech Aps Izolacja komórek płodowych za pomocą facs
CN116137894A (zh) 2020-06-10 2023-05-19 德州大学***董事会 用于确定早产风险的方法
WO2023104773A1 (en) * 2021-12-06 2023-06-15 Arcedi Biotech Aps Isolation and diagnostics of fetal cells
CN115109780B (zh) * 2022-06-22 2024-07-05 湖南大学 一种特异性识别并结合循环胎儿有核红细胞的核酸适体、互补序列及它们的应用

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SG190175A1 (en) * 2010-11-09 2013-06-28 Quantibact As Enrichment and identification of fetal cells in maternal blood and ligands for such use

Also Published As

Publication number Publication date
SG10201509230VA (en) 2015-12-30
EP2638176A1 (en) 2013-09-18
US9429520B2 (en) 2016-08-30
KR20180037308A (ko) 2018-04-11
CY1119526T1 (el) 2018-03-07
EA027314B1 (ru) 2017-07-31
JP2014503193A (ja) 2014-02-13
IL226188A0 (en) 2013-07-31
DK2638176T3 (da) 2017-11-20
PL2638176T3 (pl) 2018-01-31
HRP20171468T1 (hr) 2017-12-15
RS56402B1 (sr) 2018-01-31
EP2638176B1 (en) 2017-08-23
CN103261439B (zh) 2015-04-15
CA2817390A1 (en) 2012-05-18
ES2642313T3 (es) 2017-11-16
CA2817390C (en) 2021-02-09
ZA201303148B (en) 2014-01-29
LT2638176T (lt) 2017-11-27
PT2638176T (pt) 2017-10-17
IL226188A (en) 2017-08-31
SG190175A1 (en) 2013-06-28
JP2017060525A (ja) 2017-03-30
KR102057931B1 (ko) 2019-12-20
US20130331284A1 (en) 2013-12-12
JP6310540B2 (ja) 2018-04-11
SI2638176T1 (en) 2018-01-31
HUE034937T2 (en) 2018-03-28
KR20130102612A (ko) 2013-09-17
EA201390638A8 (ru) 2014-01-30
JP6068354B2 (ja) 2017-01-25
CN103261439A (zh) 2013-08-21
WO2012062325A1 (en) 2012-05-18

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